##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938685_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3464192 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.899794815067988 31.0 31.0 34.0 30.0 34.0 2 32.13534093953222 33.0 31.0 34.0 30.0 34.0 3 32.21306180488841 34.0 31.0 34.0 30.0 34.0 4 35.87506668221623 37.0 35.0 37.0 35.0 37.0 5 35.77951597371047 37.0 35.0 37.0 35.0 37.0 6 35.81007576947236 37.0 35.0 37.0 33.0 37.0 7 35.74336468648389 37.0 35.0 37.0 33.0 37.0 8 35.70502096881466 37.0 35.0 37.0 33.0 37.0 9 37.34373700995788 39.0 37.0 39.0 34.0 39.0 10-11 37.262375757463786 39.0 37.0 39.0 33.0 39.0 12-13 37.18150466255912 39.0 37.0 39.0 33.0 39.0 14-15 38.371716983354275 40.0 38.0 41.0 33.0 41.0 16-17 38.28724418277047 40.0 38.0 41.0 33.0 41.0 18-19 38.303591862113876 40.0 38.0 41.0 33.5 41.0 20-21 38.39482958796741 40.0 38.0 41.0 34.0 41.0 22-23 38.35007730518401 40.0 38.0 41.0 33.5 41.0 24-25 38.277512187546186 40.0 38.0 41.0 33.0 41.0 26-27 38.11165172138265 40.0 37.5 41.0 33.0 41.0 28-29 37.937063390250884 40.0 37.0 41.0 33.0 41.0 30-31 37.69725725363952 40.0 37.0 41.0 32.0 41.0 32-33 37.467123935393886 40.0 36.0 41.0 32.0 41.0 34-35 37.433410301738476 40.0 36.0 41.0 32.0 41.0 36-37 37.34825696728126 40.0 36.0 41.0 31.0 41.0 38-39 37.25112897899424 40.0 36.0 41.0 31.0 41.0 40-41 37.08693975391664 40.0 36.0 41.0 31.0 41.0 42-43 36.9059210632667 39.5 36.0 41.0 31.0 41.0 44-45 36.65079620298182 39.0 35.0 41.0 30.0 41.0 46-47 36.3585258842466 39.0 35.0 41.0 30.0 41.0 48-49 36.08168889022318 39.0 35.0 41.0 29.0 41.0 50-51 35.844140855934086 39.0 35.0 40.5 28.0 41.0 52-53 35.62788046967374 39.0 35.0 40.0 28.0 41.0 54-55 35.37876076152823 38.0 34.5 40.0 27.0 41.0 56-57 35.11766611088531 38.0 34.0 40.0 27.0 41.0 58-59 34.80520262733705 38.0 34.0 40.0 26.0 41.0 60-61 34.45934318883018 37.5 34.0 40.0 26.0 41.0 62-63 34.08050362104641 37.0 33.0 40.0 25.5 41.0 64-65 33.705247717216594 36.5 33.0 39.0 24.5 41.0 66-67 33.30308885881614 36.0 32.5 39.0 23.0 40.0 68-69 32.84316126242425 35.5 32.0 39.0 22.5 40.0 70-71 32.35504455295781 35.0 31.0 38.0 22.0 40.0 72-73 31.975941142985143 35.0 31.0 37.5 20.5 39.5 74-75 31.52383210861292 35.0 31.0 37.0 19.5 39.0 76-77 29.510478345311114 32.5 28.5 35.0 16.0 37.5 78-79 30.591300366723324 34.0 30.0 36.0 18.0 38.0 80-81 30.63958074494716 34.0 30.0 36.0 18.0 37.0 82-83 30.311518963152157 34.0 30.0 35.0 14.5 37.0 84-85 29.895684621406666 34.0 30.0 35.0 8.5 36.5 86-87 29.314081321127695 34.0 29.0 35.0 4.5 36.0 88-89 28.76159851994347 34.0 29.0 35.0 2.0 35.5 90-91 28.308333227488546 33.5 28.5 35.0 2.0 35.0 92-93 27.971734245676913 33.0 27.0 35.0 2.0 35.0 94-95 27.526760930110107 33.0 27.0 35.0 2.0 35.0 96-97 26.925276659030445 33.0 25.5 35.0 2.0 35.0 98-99 26.075746956288796 33.0 24.5 34.5 2.0 35.0 100-101 22.774257171657922 28.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02817843147787258 1101 2 0.1289836329795051 1101 3 0.09280315120392402 1101 4 0.1258948584712556 1101 5 0.04958348566854909 1101 6 0.15469910476985405 1101 7 0.15079140033024885 1101 8 0.1182383014199786 1101 9 0.15025475225268536 1101 10-11 0.2370509574040085 1101 12-13 0.2643735700888854 1101 14-15 0.3207070838696495 1101 16-17 0.2833265358894792 1101 18-19 0.2433879452536516 1101 20-21 0.2460693780025096 1101 22-23 0.26328604202969075 1101 24-25 0.3379788719059391 1101 26-27 0.37550409095862136 1101 28-29 0.39829476842078293 1101 30-31 0.3717667224291574 1101 32-33 0.3186058245632921 1101 34-35 0.32280598349866807 1101 36-37 0.34792559659278766 1101 38-39 0.46150893694121464 1101 40-41 0.49682943022755666 1101 42-43 0.4522351624207772 1101 44-45 0.5700208728804412 1101 46-47 0.5523667293501688 1101 48-49 0.5304910961630966 1101 50-51 0.6181052172177033 1101 52-53 0.59080365306707 1101 54-55 0.5364767584248966 1101 56-57 0.4770589569269248 1101 58-59 0.5941292402994804 1101 60-61 0.5870599185000955 1101 62-63 0.5778692761878688 1101 64-65 0.6087702513405233 1101 66-67 0.448299978281149 1101 68-69 0.4905705510740219 1101 70-71 0.4757012720932252 1101 72-73 0.49568354460765107 1101 74-75 0.39456349248897027 1101 76-77 0.45560817321459623 1101 78-79 0.4914869877019683 1101 80-81 0.5037422083313778 1101 82-83 0.5096314288145898 1101 84-85 0.5826717191805244 1101 86-87 0.6159160431737227 1101 88-89 0.6898350463434966 1101 90-91 0.7275107289765188 1101 92-93 0.7029968141567728 1101 94-95 0.8292348092709467 1101 96-97 0.7335159320027849 1101 98-99 0.8989058532899357 1101 100-101 1.38997154066762 1102 1 0.12077468272476821 1102 2 0.1280761137638109 1102 3 0.19483092898406085 1102 4 0.16055124201439952 1102 5 0.17600467358055738 1102 6 0.10286293843530814 1102 7 0.15708485803279615 1102 8 0.17138289498186055 1102 9 0.17741741622104712 1102 10-11 0.24406485240703546 1102 12-13 0.22607335553262686 1102 14-15 0.3038555752898233 1102 16-17 0.3826630000183613 1102 18-19 0.3146172088555872 1102 20-21 0.34522559484025805 1102 22-23 0.24142309840209464 1102 24-25 0.16139137339278165 1102 26-27 0.3415414854369061 1102 28-29 0.30072583996334146 1102 30-31 0.34341385749482356 1102 32-33 0.38545574971778507 1102 34-35 0.30229052209320173 1102 36-37 0.35575726922567696 1102 38-39 0.36227312432605885 1102 40-41 0.386530441318925 1102 42-43 0.42364215171961916 1102 44-45 0.26542256326552405 1102 46-47 0.24306955199897118 1102 48-49 0.43184013209479133 1102 50-51 0.40451196496989184 1102 52-53 0.3899866480468859 1102 54-55 0.34474436970170785 1102 56-57 0.3090339264109687 1102 58-59 0.36786306675588776 1102 60-61 0.33591694436206154 1102 62-63 0.2878023537562626 1102 64-65 0.3661950940359162 1102 66-67 0.39510108978630143 1102 68-69 0.3263294825904879 1102 70-71 0.4214028156909535 1102 72-73 0.4291084902044773 1102 74-75 0.4352578631274966 1102 76-77 0.2794079983670841 1102 78-79 0.28561779459598924 1102 80-81 0.3415611210182483 1102 82-83 0.5084220824109629 1102 84-85 0.5864429883396518 1102 86-87 0.5509675868521065 1102 88-89 0.7138989511824363 1102 90-91 0.6321739632751573 1102 92-93 0.6281033854737821 1102 94-95 0.7294010731759073 1102 96-97 0.7150687721796452 1102 98-99 0.7458880865784963 1102 100-101 1.1635246104199304 1103 1 0.019984129935888717 1103 2 0.06410021867441884 1103 3 -0.013143588007977769 1103 4 0.0516841627047242 1103 5 0.0026246960883540282 1103 6 0.0802197713047832 1103 7 0.048071124231341855 1103 8 0.09078155260048248 1103 9 -0.003723709034183287 1103 10-11 -0.019114635506817024 1103 12-13 0.0862623469271071 1103 14-15 0.06040511772107493 1103 16-17 0.011592093708763684 1103 18-19 -0.0024127807665621503 1103 20-21 0.07640963822505142 1103 22-23 0.036101039747038044 1103 24-25 -0.03909344563506778 1103 26-27 0.05666968386944404 1103 28-29 0.06358944370474973 1103 30-31 0.03938961949168629 1103 32-33 0.07397512900117675 1103 34-35 -0.11304215869363077 1103 36-37 -0.11617911759850585 1103 38-39 -0.004731847269631828 1103 40-41 0.07896813211296205 1103 42-43 0.1433902193508345 1103 44-45 0.20402824210481896 1103 46-47 0.14350134460374875 1103 48-49 0.22137236238867075 1103 50-51 0.14042407841190396 1103 52-53 0.09305253558875393 1103 54-55 0.12769808045626263 1103 56-57 0.13841094385787756 1103 58-59 0.033485855566681266 1103 60-61 0.11252083571812221 1103 62-63 0.06715885979092207 1103 64-65 0.16274496498670032 1103 66-67 0.060835069825785126 1103 68-69 0.01915844857945359 1103 70-71 -0.048823416341555514 1103 72-73 0.029062034172177675 1103 74-75 -0.001506126750733472 1103 76-77 -0.08757422956819383 1103 78-79 0.028981068133148824 1103 80-81 0.14382752677862953 1103 82-83 0.18373880894118955 1103 84-85 0.17723563859943425 1103 86-87 0.33935008299410896 1103 88-89 0.44478610556303266 1103 90-91 0.5166119084612504 1103 92-93 0.5319606681623164 1103 94-95 0.5463767307204002 1103 96-97 0.5466558416505727 1103 98-99 0.6764897237851031 1103 100-101 0.990735420565958 1104 1 -0.0292966009611213 1104 2 -0.03650111022923852 1104 3 -0.07012531558272883 1104 4 -0.0437959037404525 1104 5 -0.026880287300350858 1104 6 -0.024001158927774213 1104 7 -0.02191890899125326 1104 8 -0.02185964341280311 1104 9 -0.055773542921230046 1104 10-11 0.035429384426741706 1104 12-13 -0.06186057666094058 1104 14-15 -0.045728603209163055 1104 16-17 0.042525648526037685 1104 18-19 -0.023471750866228547 1104 20-21 -0.0027323883530172566 1104 22-23 -0.06109414297721827 1104 24-25 -0.0027587280270964243 1104 26-27 0.0418399496501749 1104 28-29 -0.013632317092593382 1104 30-31 0.05586387198337661 1104 32-33 0.011784929665630273 1104 34-35 0.04306913698742676 1104 36-37 -6.168252397031893E-4 1104 38-39 0.06607463113568457 1104 40-41 0.0898344111827285 1104 42-43 0.2072074950983449 1104 44-45 0.06816611585897903 1104 46-47 0.10789337118181663 1104 48-49 0.03750109993020345 1104 50-51 0.10921726778067153 1104 52-53 0.06971947911034704 1104 54-55 0.09553196749945414 1104 56-57 0.07402639236286035 1104 58-59 0.009437682809206649 1104 60-61 0.024297413791209976 1104 62-63 -0.07433615682037953 1104 64-65 0.10718350319268666 1104 66-67 -0.01838253814763391 1104 68-69 0.1366476512372614 1104 70-71 0.05976213183119938 1104 72-73 -0.21448032463180766 1104 74-75 -0.209782704823823 1104 76-77 -0.1251498109635456 1104 78-79 -0.03832541027216507 1104 80-81 -0.051387589500439645 1104 82-83 -0.07349707145747786 1104 84-85 0.10931288112435311 1104 86-87 0.1796233388080637 1104 88-89 0.19729275008130998 1104 90-91 0.39893084533832734 1104 92-93 0.2515661498898929 1104 94-95 0.14307855796292657 1104 96-97 0.24846315062067603 1104 98-99 0.4120254380708168 1104 100-101 0.8311050218948601 1105 1 -0.01233292477615322 1105 2 -0.01788695261941342 1105 3 0.021127876697946135 1105 4 -0.04953589299621086 1105 5 -0.02872978521348557 1105 6 -0.07026786416352593 1105 7 -0.042351240905794896 1105 8 -0.054046097398504855 1105 9 0.027228151363658526 1105 10-11 -0.0168806697077315 1105 12-13 -0.11598099486003122 1105 14-15 -0.005981712841787612 1105 16-17 0.004560691296397579 1105 18-19 -0.08696590351716793 1105 20-21 0.024712836656284765 1105 22-23 0.006059995623544978 1105 24-25 0.007895348587645401 1105 26-27 -0.025612011174651172 1105 28-29 0.01984633905022548 1105 30-31 -0.14974731029688115 1105 32-33 -0.1140193597678163 1105 34-35 -0.024728003390265485 1105 36-37 0.032533428903036565 1105 38-39 0.01636055204150466 1105 40-41 -0.10088413090578996 1105 42-43 -0.03503458102049706 1105 44-45 -0.0183842374614116 1105 46-47 0.06884039378606133 1105 48-49 0.10170772003846906 1105 50-51 0.2151405703964855 1105 52-53 0.110033459026873 1105 54-55 0.14310699126075832 1105 56-57 0.10328954373274968 1105 58-59 0.011420161424027242 1105 60-61 0.053470148201725465 1105 62-63 0.034997286710350295 1105 64-65 0.04030061547004493 1105 66-67 0.05262733829354005 1105 68-69 0.11363876657259908 1105 70-71 -0.06727708466534921 1105 72-73 -0.2162892836796999 1105 74-75 -0.024104990868700327 1105 76-77 -0.01910323879114273 1105 78-79 -0.19154364243051702 1105 80-81 -0.014140843767144418 1105 82-83 0.02469901102526606 1105 84-85 0.07988290467904235 1105 86-87 0.2009606254728027 1105 88-89 0.2770643108896067 1105 90-91 0.2993762356437131 1105 92-93 0.38469437832908326 1105 94-95 0.41587706203334207 1105 96-97 0.36037455191825174 1105 98-99 0.42875505499713285 1105 100-101 0.8127703799675103 1106 1 -0.10600312766125697 1106 2 -0.1754017367592553 1106 3 -0.13182549399856214 1106 4 -0.14750521040822662 1106 5 -0.08693771230345959 1106 6 -0.12096775782408997 1106 7 -0.15390339096295236 1106 8 -0.16115344864910242 1106 9 -0.11585229484023785 1106 10-11 -0.20333037657902508 1106 12-13 -0.21690974474524438 1106 14-15 -0.10901788581154648 1106 16-17 -0.17950190219799822 1106 18-19 -0.2357660540071791 1106 20-21 -0.23928883829285752 1106 22-23 -0.24830475664469986 1106 24-25 -0.3320694620215292 1106 26-27 -0.21098068019921357 1106 28-29 -0.31806574309151614 1106 30-31 -0.32316251696313714 1106 32-33 -0.25761310361099987 1106 34-35 -0.31708093977286467 1106 36-37 -0.1467561140658944 1106 38-39 -0.2768227175220517 1106 40-41 -0.1796939071443049 1106 42-43 -0.20913141200094287 1106 44-45 -0.09603736117517059 1106 46-47 -0.06928006560503519 1106 48-49 -0.2112452591420393 1106 50-51 -0.26983911992108034 1106 52-53 -0.2212833008572872 1106 54-55 -0.20780266591825125 1106 56-57 -0.34281685065609935 1106 58-59 -0.3240826690434062 1106 60-61 -0.3262793672552178 1106 62-63 -0.3592313893833392 1106 64-65 -0.3010070698125844 1106 66-67 -0.39304616206365495 1106 68-69 -0.4233958324356166 1106 70-71 -0.4124663234196184 1106 72-73 -0.31500416181698654 1106 74-75 -0.13894394294672097 1106 76-77 -0.23421254694960325 1106 78-79 -0.33000924081861527 1106 80-81 -0.3433893321667192 1106 82-83 -0.12155926804683403 1106 84-85 -0.1851987159048143 1106 86-87 -0.10055760804061364 1106 88-89 0.13669393675406027 1106 90-91 0.15685466592668362 1106 92-93 0.41093422590333617 1106 94-95 0.3824565505773414 1106 96-97 0.3879036717608777 1106 98-99 0.34902639238806543 1106 100-101 0.7814672475286564 1107 1 -0.05713081917736318 1107 2 0.0069829412997606255 1107 3 -0.012689912034964834 1107 4 -0.039363504678846084 1107 5 -0.03898334529104375 1107 6 -0.05224431651924988 1107 7 -0.023027111650016252 1107 8 0.012439241223155761 1107 9 -0.023585453856732386 1107 10-11 -0.00926144160116138 1107 12-13 -0.0416514973507347 1107 14-15 -0.13497809903169156 1107 16-17 -0.00905857997953774 1107 18-19 0.029951099691452043 1107 20-21 0.006982432406751116 1107 22-23 -0.02799997497294271 1107 24-25 -0.04770855722411227 1107 26-27 -0.026267635648423493 1107 28-29 0.06721922836569405 1107 30-31 -0.07467714131044545 1107 32-33 0.018188436271380226 1107 34-35 0.07555178916670968 1107 36-37 0.12322599486154218 1107 38-39 0.09441586667916368 1107 40-41 0.11471427289405511 1107 42-43 0.011757521850626063 1107 44-45 0.07765716413653934 1107 46-47 -0.10386235997840743 1107 48-49 -0.1802003446925866 1107 50-51 -0.24588360887076277 1107 52-53 -0.14573617385092064 1107 54-55 -0.05995208402954688 1107 56-57 -0.16595726817136125 1107 58-59 -0.010292242695996379 1107 60-61 -0.021442677578093594 1107 62-63 -0.019358999146589895 1107 64-65 -0.10486724381311774 1107 66-67 -0.0927331164083256 1107 68-69 0.047090257320313356 1107 70-71 -0.10718300542854564 1107 72-73 -0.07688411933897044 1107 74-75 -0.029898562748670088 1107 76-77 -0.05219994060918154 1107 78-79 -0.08372541850363291 1107 80-81 -0.19158176985192554 1107 82-83 0.057561948174573274 1107 84-85 0.017847761973840193 1107 86-87 0.05709063929783653 1107 88-89 0.1580194241562971 1107 90-91 0.282148279589471 1107 92-93 0.33172872140077914 1107 94-95 0.40826162583138625 1107 96-97 0.6011458686517095 1107 98-99 0.5287279116272217 1107 100-101 0.9669843485607075 1108 1 -0.11632926795283183 1108 2 -0.11005579899894258 1108 3 -0.15840875455558745 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8535.0 13 11799.0 14 14425.0 15 18958.0 16 23676.0 17 30348.0 18 28843.0 19 21751.0 20 20317.0 21 21462.0 22 23081.0 23 26054.0 24 30616.0 25 36489.0 26 44324.0 27 53861.0 28 65511.0 29 79592.0 30 96023.0 31 115843.0 32 141771.0 33 177783.0 34 232831.0 35 321476.0 36 472325.0 37 715908.0 38 567068.0 39 48139.0 40 5.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.04737855211664 20.064845213993262 15.02831726930436 22.85945896458574 2 16.1014458782885 21.113668064587642 39.296378491723324 23.488507565400532 3 19.456918092299734 24.879712209946792 30.145644352276086 25.517725345477388 4 13.438891377845108 17.656613721179426 36.409615864247705 32.49487903672776 5 13.55051913981673 37.56391100724209 34.397891340895654 14.487678512045521 6 31.745007205143366 36.63546939661543 17.22040810671002 14.399115291531187 7 28.08377826633166 32.83752748115578 20.773444428022465 18.305249824490097 8 25.858612917528824 35.96648222731304 19.53364594110257 18.641258914055573 9 26.02898453665386 16.83584512636713 20.26449457766775 36.87067575931126 10-11 24.835069765186223 27.05809897372894 27.238978093592962 20.86785316749187 12-13 25.718190562185928 24.31773412097251 27.83227661746231 22.13179869937925 14-15 22.5059512615029 25.712495144957042 26.289716632918914 25.491836960621146 16-17 21.95785444097025 28.943812031563056 26.895943218950535 22.20239030851616 18-19 21.722555882119035 27.270881110863016 29.55753430925185 21.4490286977661 20-21 22.500213974891402 26.420796887239057 29.740619131476997 21.338370006392545 22-23 22.130312061225244 26.569500189365947 29.470450829515222 21.829736919893588 24-25 22.05359864580254 26.66955238046852 29.61780120732338 21.659047766405557 26-27 21.889054520135744 26.914070483472607 29.43024454792869 21.76663044846296 28-29 21.708539826891812 26.94241543193911 29.613297992720955 21.735746748448122 30-31 21.84837041364913 26.735801595292642 29.63096445000739 21.784863541050843 32-33 21.620669408624003 26.894424442986992 29.469079658402304 22.015826489986697 34-35 22.188038091520866 26.927927474086083 29.224891275651387 21.65914315874166 36-37 22.13080439221338 26.694141097776175 29.2343566663895 21.940697843620942 38-39 21.898407323994245 26.93854684734336 29.429414517553443 21.73363131110895 40-41 22.09174580487276 26.771748674446172 29.384287411161225 21.752218109519838 42-43 21.9835035207837 26.86260174981904 29.095234005639313 22.058660723757946 44-45 22.054558796529907 27.109340449338305 28.91021836451204 21.925882389619748 46-47 22.09089379125674 27.137094411267938 28.531960829425906 22.240050968049417 48-49 22.268593485260123 27.29681003397475 28.374233460890792 22.06036301987434 50-51 22.124802857130074 27.440887449264622 28.236502452026464 22.19780724157884 52-53 22.417002290866094 27.37164972380284 28.094747635235002 22.116600350096068 54-55 22.117985954589123 27.306237645026606 28.288183795817325 22.287592604566953 56-57 22.17244309784215 27.68953048791753 28.04613601093704 22.091890403303278 58-59 22.28686632037034 27.31477839714807 28.127134793601314 22.271220488880275 60-61 22.157507327154075 27.30080431988162 28.45086368476827 22.09082466819604 62-63 22.21460300121933 27.24472257888708 28.36551784658587 22.175156573307714 64-65 21.995807391738104 27.211655704995568 28.304233714528525 22.488303188737806 66-67 21.93916433228103 27.53555559917694 28.07118452077161 22.45409554777042 68-69 22.01768825798443 27.328596738749987 28.19633894195206 22.45737606131352 70-71 21.89807940122305 27.27952027176825 28.167781329096385 22.65461899791232 72-73 22.025872122561335 27.222625073898904 28.13273629175288 22.618766511786877 74-75 21.945925629988174 27.37748080937777 27.943788912392847 22.732804648241206 76-77 21.95466658846294 27.29409364886997 28.1455560176831 22.605683744983995 78-79 21.96697083240265 27.32339975850206 27.982631669149843 22.726997739945443 80-81 21.799970296090816 27.437319050439058 28.004810936350065 22.75789971712006 82-83 21.945694695906003 27.33555184008277 27.890919440954775 22.82783402305646 84-85 21.8689812804833 27.241677135678394 27.85242561613213 23.03691596770618 86-87 21.890345768799257 27.15660673695421 27.9965362123647 22.956511281881838 88-89 21.873399121911582 27.097000072107384 27.921775359139666 23.107825446841364 90-91 21.789119688864357 27.234297401886533 27.940277865018054 23.03630504423106 92-93 21.80508470662134 27.06691776899202 27.98261470495862 23.145382819428022 94-95 21.607056772290203 26.955666403978995 27.96333341993366 23.473943403797133 96-97 21.745550152178367 26.791157474983702 27.955166450815433 23.5081259220225 98-99 21.5201255705703 26.75480181139156 27.897941436484025 23.82713118155412 100-101 21.61153535112394 26.38754437031571 28.38181803223486 23.61910224632549 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2716.0 1 1789.0 2 1120.0 3 1413.0 4 1185.0 5 1036.0 6 1886.5 7 4251.5 8 6459.5 9 6912.5 10 5554.0 11 3994.0 12 3674.0 13 3864.5 14 4152.0 15 4345.5 16 4580.0 17 4884.5 18 5248.5 19 5738.5 20 6408.5 21 7459.0 22 9063.0 23 10949.5 24 12988.0 25 16169.5 26 20705.5 27 25746.5 28 30529.0 29 35378.0 30 41492.0 31 47883.0 32 54492.0 33 60298.0 34 66574.5 35 73053.5 36 80197.5 37 88647.5 38 93779.0 39 96299.5 40 99637.0 41 105583.5 42 111543.0 43 116529.5 44 121247.0 45 125781.0 46 127517.5 47 129471.5 48 136715.0 49 138965.0 50 135469.0 51 131283.0 52 124344.5 53 120131.5 54 116409.5 55 107131.5 56 96356.0 57 85864.5 58 76016.0 59 66715.5 60 55922.5 61 44479.5 62 35024.5 63 26792.5 64 19984.0 65 15131.0 66 11244.0 67 8711.0 68 6310.0 69 4206.5 70 3038.5 71 2291.0 72 1772.5 73 1457.0 74 1159.0 75 910.0 76 627.5 77 362.5 78 207.0 79 118.5 80 61.5 81 37.5 82 23.5 83 16.0 84 10.0 85 6.5 86 5.5 87 5.0 88 4.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3633747783032811 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.0310098285545376E-4 16-17 0.002266040681347916 18-19 7.216690067986993E-5 20-21 1.4433380135973989E-5 22-23 0.0 24-25 0.0 26-27 1.1546704108779191E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007837325413833876 36-37 0.01755099024534437 38-39 0.011517837348507242 40-41 0.007577524571386344 42-43 0.003449577852497783 44-45 0.002193873780668046 46-47 0.0017608723765888264 48-49 4.3300140407921965E-5 50-51 4.763015444871416E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.0310098285545376E-4 60-61 6.639354862548034E-4 62-63 0.0 64-65 0.0 66-67 0.001154670410877919 68-69 8.515694280224653E-4 70-71 0.0018474726574046706 72-73 0.0 74-75 0.0 76-77 0.09082926119568431 78-79 0.0352896144324564 80-81 4.3300140407921965E-5 82-83 0.0 84-85 0.0 86-87 0.1435543988323973 88-89 0.31779416383387527 90-91 0.27403215526160213 92-93 0.0 94-95 5.7733520543895956E-5 96-97 1.4433380135973989E-5 98-99 1.2990042122376587E-4 100-101 6.783688663907774E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3464192.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.02477571045887 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52080622976396 39.719450666151054 2 15.044244193823245 16.255238366093476 3 5.26729242452444 8.53692875508996 4 2.260652853547402 4.885250534884288 5 1.125365482570534 3.0398808894082703 6 0.6777267283063623 2.1968420693840587 7 0.4167417832582101 1.5760066958791021 8 0.2958386372230973 1.278609281797251 9 0.20354184566571668 0.989667230380486 >10 1.048149945540589 10.356624840089241 >50 0.08602066479949733 3.266609661936013 >100 0.049549178629358356 5.001995824835779 >500 0.0032667364922345952 1.2578441119192065 >1k 6.426366843568078E-4 0.5948113216192003 >5k 5.355305702973398E-5 0.25674184873042377 >10k+ 1.0710611405946797E-4 0.787497901802277 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14355 0.41438234370381316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12864 0.37134200413833873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8874 0.25616363065326636 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3559 0.10273679980786284 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8866760271947975E-5 0.0 5 0.0 0.0 0.0 2.8866760271947975E-5 0.0 6 0.0 0.0 0.0 2.8866760271947975E-5 0.0 7 0.0 0.0 0.0 2.8866760271947975E-5 0.0 8 0.0 0.0 0.0 2.8866760271947975E-5 0.0 9 0.0 0.0 0.0 2.8866760271947975E-5 0.0 10-11 0.0 0.0 0.0 5.773352054389595E-5 0.0 12-13 0.0 0.0 0.0 1.0103366095181791E-4 0.0 14-15 0.0 0.0 0.0 1.4433380135973987E-4 0.0 16-17 0.0 0.0 0.0 1.5876718149571386E-4 0.0 18-19 0.0 0.0 0.0 2.1650070203960983E-4 0.0 20-21 0.0 0.0 0.0 3.1753436299142773E-4 0.0 22-23 0.0 0.0 0.0 5.196016848950636E-4 0.0 24-25 0.0 0.0 0.0 7.216690067986994E-4 0.0 26-27 0.0 0.0 0.0 0.0011258036506059709 0.0 28-29 0.0 0.0 0.0 0.0028866760271947975 0.0 30-31 0.0 0.0 0.0 0.00803939273573751 0.0 32-33 0.0 0.0 0.0 0.01864792713567839 0.0 34-35 0.0 0.0 0.0 0.03722368737067691 0.0 36-37 0.0 0.0 0.0 0.06809668748152527 0.0 38-39 0.0 0.0 0.0 0.1118009625332545 0.0 40-41 0.0 0.0 0.0 0.15909914923884128 0.0 42-43 0.0 0.0 0.0 0.20638290256429204 0.0 44-45 0.0 0.0 0.0 0.2739888551211942 0.0 46-47 0.0 0.0 0.0 0.35804886103310674 0.0 48-49 0.0 0.0 0.0 0.44848842096511976 0.0 50-51 0.0 0.0 0.0 0.5446868995713864 0.0 52-53 0.0 0.0 0.0 0.6431225520987289 2.8866760271947975E-5 54-55 0.0 0.0 0.0 0.756323552505173 2.8866760271947975E-5 56-57 0.0 0.0 0.0 0.892820028451079 2.8866760271947975E-5 58-59 0.0 0.0 0.0 1.043186982707656 2.8866760271947975E-5 60-61 0.0 0.0 0.0 1.2074965821755836 2.8866760271947975E-5 62-63 0.0 0.0 0.0 1.376497030187703 2.8866760271947975E-5 64-65 0.0 0.0 0.0 1.556336946681939 2.8866760271947975E-5 66-67 0.0 0.0 0.0 1.7630518169893585 2.8866760271947975E-5 68-69 0.0 0.0 0.0 2.0020830254212236 2.8866760271947975E-5 70-71 0.0 0.0 0.0 2.2660551147280517 2.8866760271947975E-5 72-73 0.0 0.0 0.0 2.529493168969849 2.8866760271947975E-5 74-75 0.0 0.0 0.0 2.798286007242093 2.8866760271947975E-5 76-77 0.0 0.0 0.0 3.0950796029781262 2.8866760271947975E-5 78-79 0.0 0.0 0.0 3.4356207739062965 2.8866760271947975E-5 80-81 0.0 0.0 0.0 3.804826637784511 2.8866760271947975E-5 82-83 0.0 0.0 0.0 4.189952519952705 2.8866760271947975E-5 84-85 0.0 0.0 0.0 4.570762821460242 2.8866760271947975E-5 86-87 0.0 0.0 0.0 4.970827252069169 2.8866760271947975E-5 88-89 0.0 0.0 0.0 5.409977276086314 2.8866760271947975E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7980 0.0 52.81718 1 GTATCAA 13410 0.0 37.148197 1 ATCAACG 17190 0.0 28.821373 3 TCAACGC 17370 0.0 28.522709 4 TATCAAC 17590 0.0 28.273886 2 CAACGCA 17890 0.0 27.720179 5 AACGCAG 18405 0.0 26.91874 6 ACGCAGA 20920 0.0 23.478415 7 CGCAGAG 21060 0.0 23.209667 8 GTACATG 13415 0.0 23.11134 1 TACATGG 13705 0.0 21.814817 2 GAGTACT 13985 0.0 21.548964 12-13 GCAGAGT 22720 0.0 21.263552 9 GTGGTAT 4000 0.0 20.954992 1 ACATGGG 13550 0.0 20.873583 3 TGGTATC 3925 0.0 20.675047 2 GTACTTT 14955 0.0 20.26234 14-15 AGTACTT 14605 0.0 19.756824 12-13 CAGAGTA 22590 0.0 19.748024 10-11 TACCTGG 2705 0.0 19.47358 2 >>END_MODULE