FastQCFastQC Report
Thu 26 May 2016
SRR938684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458554
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146920.42480181023630104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118120.34153001514505776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78220.2261638823623977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72600.049.690731
GTATCAA127250.039.2941741
ATCAACG156800.031.5262853
TCAACGC158350.031.247654
TATCAAC160150.031.0445582
CAACGCA163500.030.2924125
AACGCAG168300.029.5399286
ACGCAGA191350.025.7839647
CGCAGAG193650.025.3552388
GTACATG137500.024.7157361
TACATGG139150.024.0698072
GTGGTAT40800.023.4156461
ACATGGG138950.023.2508953
TGGTATC39850.022.9763012
GCAGAGT211300.022.7417229
GAGTACT125250.021.43067412-13
GTACTTT133550.020.09892514-15
CAGAGTA207700.019.80045710-11
CATGGGG64900.018.8593244
AGAGTAC196800.018.85926610-11
Produced by FastQC (version 0.11.5)