##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938684_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3458554 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.728306396256933 33.0 31.0 34.0 30.0 34.0 2 31.819562163840725 33.0 31.0 34.0 30.0 34.0 3 31.83960464402175 34.0 31.0 34.0 30.0 34.0 4 35.38501205995338 37.0 35.0 37.0 33.0 37.0 5 35.413707000093105 37.0 35.0 37.0 33.0 37.0 6 35.465752739439665 37.0 35.0 37.0 33.0 37.0 7 35.380352598224576 37.0 35.0 37.0 33.0 37.0 8 35.353414461650736 37.0 35.0 37.0 33.0 37.0 9 36.965526344246754 39.0 37.0 39.0 33.0 39.0 10-11 36.85456537616588 39.0 37.0 39.0 33.0 39.0 12-13 36.74386709011917 39.0 37.0 39.0 33.0 39.0 14-15 37.9524467450848 40.0 38.0 41.0 33.0 41.0 16-17 37.83362078487137 40.0 38.0 41.0 33.0 41.0 18-19 37.77219395735906 40.0 38.0 41.0 32.5 41.0 20-21 37.669991562947985 40.0 37.5 41.0 32.0 41.0 22-23 37.69155172942218 40.0 38.0 41.0 32.0 41.0 24-25 37.63321376505904 40.0 38.0 41.0 32.0 41.0 26-27 37.5104435263986 40.0 37.0 41.0 32.0 41.0 28-29 37.34232239832022 40.0 37.0 41.0 31.5 41.0 30-31 37.124072372442356 40.0 37.0 41.0 31.0 41.0 32-33 36.94682156184348 40.0 36.0 41.0 30.5 41.0 34-35 36.6730195625108 39.0 36.0 41.0 30.0 41.0 36-37 36.47313371426324 39.0 35.5 41.0 30.0 41.0 38-39 36.31510668909608 39.0 35.0 41.0 30.0 41.0 40-41 36.21083435447299 39.0 35.0 41.0 30.0 41.0 42-43 36.21325588670872 39.0 35.0 41.0 30.0 41.0 44-45 36.04889124183112 39.0 35.0 41.0 29.0 41.0 46-47 35.7613212053361 39.0 35.0 41.0 27.5 41.0 48-49 35.496596843652 39.0 35.0 41.0 26.0 41.0 50-51 34.385142027564115 38.0 34.0 40.0 24.5 40.5 52-53 34.4694203126509 38.0 34.0 39.5 24.0 40.5 54-55 34.964838629091815 39.0 35.0 40.0 25.5 41.0 56-57 34.86916989007545 39.0 35.0 40.0 25.0 41.0 58-59 34.66773830913151 38.0 34.0 40.0 24.5 41.0 60-61 34.371252407798174 38.0 34.0 40.0 23.5 41.0 62-63 33.99042649037719 37.5 34.0 40.0 22.5 41.0 64-65 33.6028238969234 37.0 33.0 40.0 22.0 41.0 66-67 33.227010334376736 36.5 33.0 39.5 20.5 41.0 68-69 32.807691451398476 36.0 32.5 39.0 19.5 41.0 70-71 32.3881296923512 35.5 32.0 39.0 18.0 40.0 72-73 31.961997846498853 35.0 32.0 38.0 16.0 40.0 74-75 31.488365658017774 35.0 31.0 37.0 12.0 39.5 76-77 31.031312508059727 35.0 31.0 37.0 7.5 39.0 78-79 30.595186022829193 35.0 31.0 36.0 7.0 39.0 80-81 30.13190136687182 34.0 30.0 36.0 2.0 37.5 82-83 29.721721852543 34.0 30.0 35.0 2.0 37.0 84-85 29.374040133535576 34.0 29.5 35.0 2.0 37.0 86-87 28.9524531061247 34.0 29.0 35.0 2.0 36.0 88-89 28.522579233980444 34.0 29.0 35.0 2.0 36.0 90-91 28.083488504155206 34.0 28.5 35.0 2.0 35.5 92-93 27.630068809103456 34.0 27.0 35.0 2.0 35.0 94-95 27.128744411681875 33.5 26.0 35.0 2.0 35.0 96-97 26.54351442828419 33.0 25.0 35.0 2.0 35.0 98-99 25.79371451190295 33.0 23.5 35.0 2.0 35.0 100-101 23.313989748316782 30.0 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10390176430959741 1101 2 0.22663087758594358 1101 3 0.3187362131792604 1101 4 0.115565756547376 1101 5 0.06126728315577168 1101 6 0.16398561282559143 1101 7 0.1807224004838659 1101 8 0.24052441464834828 1101 9 0.2978537601967659 1101 10-11 0.16283285784297874 1101 12-13 0.20745664442187461 1101 14-15 0.19996238067096073 1101 16-17 0.35865291480290296 1101 18-19 0.2504182126749299 1101 20-21 0.3714391396745995 1101 22-23 0.37144655199669785 1101 24-25 0.3723040272013307 1101 26-27 0.3433029255743989 1101 28-29 0.31533590640604103 1101 30-31 0.5084610696264207 1101 32-33 0.41130047251868973 1101 34-35 0.40926640998021213 1101 36-37 0.48876062023840916 1101 38-39 0.5418090136261284 1101 40-41 0.5132353941191496 1101 42-43 0.6262902957070224 1101 44-45 0.6816249759838016 1101 46-47 0.5196728033744975 1101 48-49 0.6516196233211033 1101 50-51 0.725406291247829 1101 52-53 0.7256041111181517 1101 54-55 0.7479427378393382 1101 56-57 0.7297690102833414 1101 58-59 0.7704235029211546 1101 60-61 0.7735241706547882 1101 62-63 0.562456276318116 1101 64-65 0.5158286987944152 1101 66-67 0.47836435716924086 1101 68-69 0.6433982824309936 1101 70-71 0.6223867401233818 1101 72-73 0.5795886538887949 1101 74-75 0.6113768416555949 1101 76-77 0.44850150824768775 1101 78-79 0.3440383252948358 1101 80-81 0.39369470208264445 1101 82-83 0.3883661090668582 1101 84-85 0.1939959889950309 1101 86-87 0.09840869105545025 1101 88-89 0.22227193499515963 1101 90-91 0.2009108846296499 1101 92-93 0.01019931182741729 1101 94-95 -0.08635512240030963 1101 96-97 -0.08235278587475392 1101 98-99 -0.025903872439027253 1101 100-101 0.3899927218715469 1102 1 0.09170760835113967 1102 2 0.13631451202930478 1102 3 0.17014497604111156 1102 4 0.1954338175481567 1102 5 0.12995597432730221 1102 6 0.1571121430459428 1102 7 0.03580449230508265 1102 8 0.05992720865068435 1102 9 0.18498310682268482 1102 10-11 0.26329356418469274 1102 12-13 0.22608168642792492 1102 14-15 0.341537447480583 1102 16-17 0.49201183848791885 1102 18-19 0.43709045347760167 1102 20-21 0.3821167972572894 1102 22-23 0.37516600759830965 1102 24-25 0.311180425402668 1102 26-27 0.3304655969990833 1102 28-29 0.46106017070009386 1102 30-31 0.41788170619808085 1102 32-33 0.378718380697471 1102 34-35 0.4306035225244145 1102 36-37 0.5516683989585474 1102 38-39 0.4701016477654463 1102 40-41 0.5411137686050793 1102 42-43 0.5452359838861156 1102 44-45 0.37247281807306365 1102 46-47 0.47072143437736 1102 48-49 0.5021338973765594 1102 50-51 0.45324894058934007 1102 52-53 0.34533263202516196 1102 54-55 0.37780599814882265 1102 56-57 0.5216051546881388 1102 58-59 0.4482654721647279 1102 60-61 0.4779872933444551 1102 62-63 0.3743239452624394 1102 64-65 0.19528053985951743 1102 66-67 0.21453948638784937 1102 68-69 0.2847857622280898 1102 70-71 0.20458661690563673 1102 72-73 0.22075898241610403 1102 74-75 0.19031424864219915 1102 76-77 0.24260209873271776 1102 78-79 0.14863150671295955 1102 80-81 0.21791506981560005 1102 82-83 0.31791868463774264 1102 84-85 0.1303834687921217 1102 86-87 0.07745743407444294 1102 88-89 0.10544647212850577 1102 90-91 0.08917971534127389 1102 92-93 0.01293038510196709 1102 94-95 -0.04240259323611184 1102 96-97 -0.292236249093456 1102 98-99 -0.4785152884829351 1102 100-101 -0.1622229571862377 1103 1 0.14708532093580473 1103 2 0.280662020942156 1103 3 0.24509785266532802 1103 4 0.28974944023170224 1103 5 0.3545766144835554 1103 6 0.27003967541261886 1103 7 0.20709770834311314 1103 8 0.2340162155495591 1103 9 0.3828055872026539 1103 10-11 0.3902187590122992 1103 12-13 0.4014367688104912 1103 14-15 0.4899503133583991 1103 16-17 0.47783254610335035 1103 18-19 0.5049324656437904 1103 20-21 0.6011546802940515 1103 22-23 0.5165681780582432 1103 24-25 0.5145078887413561 1103 26-27 0.5031013684065826 1103 28-29 0.43956649892039223 1103 30-31 0.6488055832184259 1103 32-33 0.5553892700997096 1103 34-35 0.6198953187820067 1103 36-37 0.6200344356139809 1103 38-39 0.5443301225996322 1103 40-41 0.5443096865401955 1103 42-43 0.6058055433227594 1103 44-45 0.6521719579684984 1103 46-47 0.5058411155935048 1103 48-49 0.520693212430551 1103 50-51 0.5008491978755174 1103 52-53 0.5713592470254198 1103 54-55 0.5529853728963374 1103 56-57 0.5345532777933002 1103 58-59 0.5522108929805896 1103 60-61 0.45743550077359885 1103 62-63 0.3294234279446471 1103 64-65 0.35718845807703303 1103 66-67 0.3772097325746415 1103 68-69 0.3596414113462174 1103 70-71 0.44210290319927026 1103 72-73 0.5046238836393862 1103 74-75 0.445297364236648 1103 76-77 0.42548409615201166 1103 78-79 0.5017707903121398 1103 80-81 0.39410876395096395 1103 82-83 0.45309676511027064 1103 84-85 0.4208252087683526 1103 86-87 0.39572067391928556 1103 88-89 0.48426256349647545 1103 90-91 0.4402112917289891 1103 92-93 0.3351173176310205 1103 94-95 0.49347804323342004 1103 96-97 0.5222716322568246 1103 98-99 0.5763718154395008 1103 100-101 0.7008009398365367 1104 1 0.15307071008121298 1104 2 0.2015432282671945 1104 3 0.20776264888813856 1104 4 0.2665965386191331 1104 5 0.264174660839565 1104 6 0.27306090376534087 1104 7 0.3554135682444368 1104 8 0.28114146839705256 1104 9 0.35025758769349125 1104 10-11 0.3779095903603462 1104 12-13 0.3982944278149816 1104 14-15 0.5038397934526628 1104 16-17 0.5053066782047679 1104 18-19 0.46163740499703465 1104 20-21 0.5485221350387732 1104 22-23 0.4381881841984665 1104 24-25 0.419621220082 1104 26-27 0.4959855816016372 1104 28-29 0.5002663467870079 1104 30-31 0.4395219619667827 1104 32-33 0.42393554885042306 1104 34-35 0.5782321018961127 1104 36-37 0.6514134856761089 1104 38-39 0.5274836541382726 1104 40-41 0.5399020750998034 1104 42-43 0.6717054776950562 1104 44-45 0.7078771079727701 1104 46-47 0.712181625562323 1104 48-49 0.7994799763740232 1104 50-51 0.8758438380466202 1104 52-53 0.9068116423115171 1104 54-55 0.7773620384388096 1104 56-57 0.7240020238740001 1104 58-59 0.7627335117703851 1104 60-61 0.7456124510613549 1104 62-63 0.6483651334320726 1104 64-65 0.8843269674380636 1104 66-67 0.7625021048091867 1104 68-69 0.7288263088374407 1104 70-71 0.872114257460801 1104 72-73 0.606133546689744 1104 74-75 0.6861924377313962 1104 76-77 0.7225867012861258 1104 78-79 0.5095964133659372 1104 80-81 0.5624846897980653 1104 82-83 0.5596945234778019 1104 84-85 0.6312102261326409 1104 86-87 0.5825886331505963 1104 88-89 0.6431667946194679 1104 90-91 0.6695667900402711 1104 92-93 0.7839985829591534 1104 94-95 0.6736599375053167 1104 96-97 0.5175505390419559 1104 98-99 0.8738924872275895 1104 100-101 1.139202589743899 1105 1 -0.03462948945410105 1105 2 0.06262991407878005 1105 3 0.16430106925537658 1105 4 0.10852914327506369 1105 5 0.16252285343157524 1105 6 0.10554288128277278 1105 7 0.09455776119686021 1105 8 0.13034737020657872 1105 9 0.11640127314389304 1105 10-11 0.23785393456223858 1105 12-13 0.16935445244308056 1105 14-15 0.21725582882223904 1105 16-17 0.2752749188978143 1105 18-19 0.31368751292784935 1105 20-21 0.3530888446005278 1105 22-23 0.26688946419710646 1105 24-25 0.2120637526017859 1105 26-27 0.20951543912371307 1105 28-29 0.3837479261579304 1105 30-31 0.3019965387339667 1105 32-33 0.3707684359054184 1105 34-35 0.20042472625144114 1105 36-37 0.3971973298614344 1105 38-39 0.28838555222662876 1105 40-41 0.2803602285163578 1105 42-43 0.28881287790622423 1105 44-45 0.4997042846674091 1105 46-47 0.4579240378680538 1105 48-49 0.4921034845760701 1105 50-51 0.49518528679161733 1105 52-53 0.49098108329688017 1105 54-55 0.4558103760424004 1105 56-57 0.42775919455409195 1105 58-59 0.444235679242972 1105 60-61 0.4156625543715222 1105 62-63 0.4559962618655007 1105 64-65 0.4018909654450127 1105 66-67 0.3076489530984219 1105 68-69 0.3120331132150511 1105 70-71 0.3430012770377502 1105 72-73 0.37772486971440244 1105 74-75 0.33882324656947915 1105 76-77 0.2684099750669091 1105 78-79 0.20543932493351846 1105 80-81 0.27252464306783253 1105 82-83 0.2196861138843964 1105 84-85 0.1438635067596934 1105 86-87 0.21730246439038936 1105 88-89 0.3028753313579209 1105 90-91 0.39155733771889345 1105 92-93 0.3662498357344397 1105 94-95 0.40144898454898126 1105 96-97 0.27580842091797564 1105 98-99 0.33850658161372493 1105 100-101 0.8196326110682257 1106 1 0.08611126831496563 1106 2 0.12224570393560086 1106 3 0.17256457580510798 1106 4 0.16592158704007431 1106 5 0.21509849432459305 1106 6 0.2234104919998927 1106 7 0.15419194164689287 1106 8 0.17503229719975621 1106 9 0.2147541656393841 1106 10-11 0.17354068912061393 1106 12-13 0.21842381179909864 1106 14-15 0.27509788427753534 1106 16-17 0.2907527103408185 1106 18-19 0.2928155306134741 1106 20-21 0.308225184029574 1106 22-23 0.24922146804380674 1106 24-25 0.14850547932523028 1106 26-27 0.11307612616563034 1106 28-29 0.053679114688328866 1106 30-31 -0.06568927330650354 1106 32-33 0.08663798529757116 1106 34-35 0.18080847662978528 1106 36-37 0.3640325769658759 1106 38-39 0.42542822989700113 1106 40-41 0.5481257331274136 1106 42-43 0.49832828577510213 1106 44-45 0.48233034643681805 1106 46-47 0.45662979987078955 1106 48-49 0.4974834993651669 1106 50-51 0.47678664769551204 1106 52-53 0.377177218973479 1106 54-55 0.29380151024392376 1106 56-57 0.46065785855412145 1106 58-59 0.5967586387491863 1106 60-61 0.6221707171129438 1106 62-63 0.5885581481273476 1106 64-65 0.6080807410414693 1106 66-67 0.6382897886443715 1106 68-69 0.7245764556287426 1106 70-71 0.7877486554203799 1106 72-73 0.7642355611623834 1106 74-75 0.6041826485686919 1106 76-77 0.65192165884066 1106 78-79 0.6842612158696362 1106 80-81 0.6998800757640105 1106 82-83 0.6579124944240817 1106 84-85 0.9003816310724382 1106 86-87 0.8998927028167678 1106 88-89 0.9644958874434799 1106 90-91 1.069289011301187 1106 92-93 1.1808958359719846 1106 94-95 1.3109241629952102 1106 96-97 1.2942735343457912 1106 98-99 1.2765762589737548 1106 100-101 1.6008866579565755 1107 1 0.10627021110271784 1107 2 0.14066150362038954 1107 3 0.17931124284516997 1107 4 0.1687890449978724 1107 5 0.09158410997118693 1107 6 -0.022012590488238004 1107 7 0.030205209697925284 1107 8 0.10239773025506338 1107 9 0.06069440340012022 1107 10-11 0.08496240715274439 1107 12-13 0.07102320160566222 1107 14-15 0.18062210298256076 1107 16-17 0.11299034816720877 1107 18-19 0.14647264608814936 1107 20-21 0.20023930467113615 1107 22-23 0.31950304851014977 1107 24-25 0.1091509588062749 1107 26-27 0.13926337402520517 1107 28-29 0.2670287297001508 1107 30-31 0.21962574955630743 1107 32-33 0.2628159760155384 1107 34-35 0.31393924192312994 1107 36-37 0.34247806173670625 1107 38-39 0.22521901071745276 1107 40-41 0.22504503418988264 1107 42-43 0.24019067486442935 1107 44-45 0.45297011605067183 1107 46-47 0.584535297679416 1107 48-49 0.45120175864914813 1107 50-51 0.5095890178349336 1107 52-53 0.4371088872659854 1107 54-55 0.4512687951943022 1107 56-57 0.47162037568761406 1107 58-59 0.49971897928752895 1107 60-61 0.4696300206958526 1107 62-63 0.48361685707504165 1107 64-65 0.3974867981681456 1107 66-67 0.25028688859194403 1107 68-69 0.5432150101455306 1107 70-71 0.5801702458751024 1107 72-73 0.5045667101214697 1107 74-75 0.5258699921113283 1107 76-77 0.48658681480404553 1107 78-79 0.4303650094307123 1107 80-81 0.4397881966887276 1107 82-83 0.40218489087245146 1107 84-85 0.6742401632787356 1107 86-87 0.556197827539247 1107 88-89 0.6016127467987182 1107 90-91 0.7505280913746866 1107 92-93 0.8356229681095115 1107 94-95 0.9400232486385498 1107 96-97 0.9972327858086487 1107 98-99 1.055318633500594 1107 100-101 1.5383557554397207 1108 1 0.21614861524187035 1108 2 0.18983074594773441 1108 3 0.14734768801530507 1108 4 0.192540817799582 1108 5 0.23802627088022632 1108 6 0.19536717259895653 1108 7 0.1283517622198147 1108 8 0.1735032730494126 1108 9 0.2833122000329453 1108 10-11 0.3531191012314352 1108 12-13 0.35222948135086085 1108 14-15 0.4065012892451634 1108 16-17 0.514286078568368 1108 18-19 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-0.9280074691838607 2316 82-83 -0.852915096965269 2316 84-85 -0.8293852240534925 2316 86-87 -0.9211385852908087 2316 88-89 -0.9405675138854797 2316 90-91 -1.2547578855194068 2316 92-93 -1.064810335715677 2316 94-95 -1.0476742446616107 2316 96-97 -1.1656305471031345 2316 98-99 -1.4457772572833676 2316 100-101 -1.4321315101717111 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17881.0 3 4153.0 4 3737.0 5 4057.0 6 4619.0 7 5404.0 8 6711.0 9 7564.0 10 9263.0 11 11151.0 12 13911.0 13 17254.0 14 18655.0 15 20944.0 16 24944.0 17 30363.0 18 27372.0 19 20887.0 20 20714.0 21 21961.0 22 24016.0 23 26690.0 24 30182.0 25 34563.0 26 40007.0 27 47086.0 28 55690.0 29 66962.0 30 80316.0 31 99739.0 32 124566.0 33 160012.0 34 215133.0 35 307431.0 36 452410.0 37 676927.0 38 623967.0 39 101266.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80394460208989 18.81812109566611 14.19867152629522 23.179262775948782 2 15.32366758586257 20.092365577011183 40.8208413625414 23.763125474584843 3 19.282161123689768 24.531148973249564 29.671882495533097 26.514807407527574 4 12.637638231905832 16.565182211559904 37.26268246785328 33.53449708868098 5 13.662759358586321 37.16184416285963 34.41324303865847 14.76215343989558 6 31.913635661818272 35.84202883310984 17.842670186870325 14.401665318201557 7 28.400283665498442 32.59568131740424 20.866281046200555 18.137753970896757 8 25.914081617333572 35.27358758793747 19.979880132891985 18.832450661836972 9 26.393981001842135 16.865338698945273 20.48266764110936 36.258012658103226 10-11 24.764975152167352 26.68454573016646 27.546408525978215 21.004070591687974 12-13 25.80211411234965 24.071079418789317 27.912532436800664 22.21427403206037 14-15 22.68994606999692 25.40874690722829 26.422863239756385 25.478443783018406 16-17 22.1270130016231 28.684276450193302 26.943995193905167 22.244715354278426 18-19 21.869352357540496 27.035672416923283 29.53333131449623 21.56164391103999 20-21 22.673479804794844 26.237587479337815 29.65069389604393 21.438238819823415 22-23 22.300972185028165 26.42225244118605 29.39223654724766 21.884538826538126 24-25 22.132551362479308 26.46677493242084 29.59638260236562 21.804291102734233 26-27 21.975063699894083 26.73528723190997 29.374042470850938 21.915606597345015 28-29 21.787281914956093 26.796221166761963 29.564481740187887 21.852015178094057 30-31 21.906537091690005 26.637150680419403 29.50924006749466 21.947072160395933 32-33 21.653305810679697 26.786921853729513 29.430449368827556 22.129322966763237 34-35 22.262200302222098 26.791426130837564 29.166478068006935 21.779895498933403 36-37 22.165108774292708 26.59159369450893 29.136097216547885 22.107200314650477 38-39 21.94202105291263 26.822046847388588 29.309611779786916 21.926320319911866 40-41 22.158756701799877 26.654309771728656 29.32945954637585 21.857473980095612 42-43 21.97976334903471 26.758329847282685 29.10551205186837 22.156394751814233 44-45 22.079261454661967 26.981275162642966 28.91453234052631 22.024931042168756 46-47 22.099356913836555 27.023869679635293 28.519494157020763 22.35727924950739 48-49 22.267027658301185 27.117626558866547 28.386172605693183 22.229173177139085 50-51 22.1531315824727 27.34507472659947 28.24567201399481 22.25612167693302 52-53 22.406176193591097 27.252484699354966 28.184884650647184 22.15645445640675 54-55 22.127804086561138 27.133427861122524 28.384358505106388 22.354409547209954 56-57 22.155147146310533 27.54990728084762 28.163928622213707 22.13101695062814 58-59 22.248222971829104 27.18483544600908 28.22301825037924 22.343923331782573 60-61 22.182670373383566 27.108688306584355 28.56767876280347 22.140962557228605 62-63 22.214183005938427 27.095365856171455 28.493449417294674 22.19700172059544 64-65 22.000882282367808 27.057457659373686 28.45029671426027 22.491363343998238 66-67 21.968927577232485 27.37386608970942 28.171551873310968 22.485654459747128 68-69 22.07778387837544 27.16406604092149 28.332090147025045 22.42605993367802 70-71 21.937940685056255 27.157965826307027 28.27339651213416 22.630696976502556 72-73 22.096477617174813 27.09922772092972 28.239813111977924 22.564481549917538 74-75 22.006469861263483 27.240333891987632 28.14020955197241 22.61298669477647 76-77 22.097242222279633 27.153351877389788 28.158147343684377 22.59125855664621 78-79 22.067894696214747 27.218355207295282 28.10729626686833 22.606453829621636 80-81 21.962612957287188 27.32422501647388 28.095352181205886 22.617809845033044 82-83 22.167058528839927 27.254421710262513 27.982079362285127 22.59644039861243 84-85 22.112244030164174 27.131924584337003 27.94172837671492 22.814103008783903 86-87 22.11009793400393 27.034545009951582 28.162574654996142 22.692782401048344 88-89 22.151910859085465 26.987909386871756 27.99583298501779 22.864346769024994 90-91 22.060398101755695 27.113329690754334 28.076198610749014 22.750073596740954 92-93 22.16835342344853 26.945692818654777 28.054002345782237 22.831951412114456 94-95 22.022119907989776 26.86621571301753 28.054218961974286 23.057445417018407 96-97 22.221433447178253 26.662778925130926 28.022051246762995 23.093736380927826 98-99 22.043118537144306 26.615391829192397 27.943228270774227 23.39826136288907 100-101 22.312354060656777 26.21257461782741 28.303259796376835 23.171811525138974 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4990.0 1 3277.5 2 1732.5 3 1952.0 4 1702.0 5 1559.5 6 2436.0 7 4617.5 8 6891.5 9 7395.5 10 5942.5 11 4504.0 12 4271.0 13 4410.5 14 4561.0 15 4703.0 16 4904.0 17 5215.0 18 5503.5 19 5817.5 20 6481.5 21 7643.0 22 9068.0 23 10728.0 24 12833.0 25 15990.0 26 20232.0 27 25354.0 28 30216.5 29 34887.5 30 40690.5 31 47174.0 32 53705.0 33 59460.0 34 66062.5 35 72360.5 36 79107.5 37 87339.5 38 92833.5 39 95437.5 40 98473.5 41 104320.0 42 110493.5 43 115732.0 44 120097.5 45 124617.0 46 126992.0 47 128435.5 48 134847.0 49 137086.0 50 133957.0 51 130268.0 52 123704.5 53 119404.0 54 115136.0 55 106219.5 56 96442.0 57 86378.0 58 76490.0 59 67188.0 60 56652.5 61 45495.0 62 36208.5 63 27836.0 64 20627.0 65 15679.0 66 11890.0 67 9323.0 68 6937.0 69 4865.0 70 3664.5 71 2743.0 72 2088.0 73 1674.5 74 1336.5 75 1110.5 76 788.0 77 505.0 78 336.5 79 233.0 80 173.5 81 122.5 82 105.5 83 87.0 84 70.5 85 58.0 86 42.0 87 30.5 88 21.0 89 14.0 90 12.5 91 10.5 92 9.5 93 6.5 94 5.5 95 6.5 96 5.0 97 3.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2654866744888182 2 0.16006689500872331 3 0.18788198767461778 4 0.15844772121528244 5 0.22917091940735926 6 0.18739045277303754 7 0.23162859391526056 8 0.23804746145354386 9 0.14329688071951457 10-11 0.19790062552153298 12-13 0.20339425089213586 14-15 0.16610988291638645 16-17 0.27757265030414446 18-19 0.2232869574972662 20-21 0.19071554181313927 22-23 0.2365728567488031 24-25 0.22512298492375718 26-27 0.3334052323601135 28-29 0.30528654460794885 30-31 0.2801315231741358 32-33 0.1841087344595458 34-35 0.3493367459348618 36-37 0.38922335750721254 38-39 0.3716871270478934 40-41 0.33613758813654493 42-43 0.36655492439904075 44-45 0.3750122160879952 46-47 0.3798408236505777 48-49 0.3985336068194974 50-51 0.3921436530989541 52-53 0.39090035893613345 54-55 0.386259691188861 56-57 0.3661645878595506 58-59 0.3428745076699684 60-61 0.3110837650648219 62-63 0.37427491373562477 64-65 0.40706318305280187 66-67 0.4128314896919349 68-69 0.4177179248899974 70-71 0.4167493119957069 72-73 0.4087980121172027 74-75 0.4038392923747902 76-77 0.388168003159702 78-79 0.3643141035241896 80-81 0.37439056900658485 82-83 0.38318036959954943 84-85 0.3838309304986997 86-87 0.3793926594756074 88-89 0.38750298535168165 90-91 0.4079161406761323 92-93 0.40693307087297176 94-95 0.4155349316506262 96-97 0.4162144063675166 98-99 0.4135977058620452 100-101 0.3878933218911718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3458554.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.52712298830114 #Duplication Level Percentage of deduplicated Percentage of total 1 74.73084875725313 41.49589029964125 2 14.620242918118098 16.236400532663644 3 4.828264010370051 8.042988285714177 4 2.1052719220326157 4.675987717540887 5 1.0781678706622777 2.993377997814952 6 0.6362495107997063 2.1197462902453035 7 0.4069066183367299 1.581604768879612 8 0.2692569359583699 1.1960850398730822 9 0.1975270133669252 0.9871296088263353 >10 0.9967876432101133 9.984632955428326 >50 0.08052661856857041 3.136733088719712 >100 0.045874970624497476 4.6901128355228066 >500 0.003291516336520174 1.2820190141034375 >1k 6.269554899294728E-4 0.5814647103648177 >5k 5.2246290827456065E-5 0.2269229638513997 >10k+ 1.0449258165491213E-4 0.7689038908102145 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14692 0.42480181023630104 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11812 0.34153001514505776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7822 0.2261638823623977 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.7827635480030096E-5 0.0 8 0.0 0.0 0.0 5.7827635480030096E-5 0.0 9 0.0 0.0 0.0 5.7827635480030096E-5 0.0 10-11 0.0 0.0 0.0 8.674145322004514E-5 0.0 12-13 0.0 0.0 0.0 1.1565527096006019E-4 0.0 14-15 0.0 0.0 0.0 1.1565527096006019E-4 0.0 16-17 0.0 0.0 0.0 1.4456908870007524E-4 0.0 18-19 0.0 0.0 0.0 1.7348290644009028E-4 0.0 20-21 0.0 0.0 0.0 2.746812685301429E-4 0.0 22-23 0.0 0.0 0.0 5.059918104502633E-4 0.0 24-25 0.0 0.0 0.0 7.228454435003761E-4 0.0 26-27 0.0 0.0 0.0 0.001069811256380557 0.0 28-29 0.0 0.0 0.0 0.002602243596601354 0.0 30-31 0.0 0.0 0.0 0.006997143893083641 0.0 32-33 0.0 0.0 0.0 0.01733383373513902 0.0 34-35 0.0 0.0 0.0 0.03281718313491708 0.0 36-37 0.0 0.0 0.0 0.060849129433861664 0.0 38-39 0.0 0.0 0.0 0.10135738808762274 0.0 40-41 0.0 0.0 0.0 0.1458557535895059 0.0 42-43 0.0 0.0 0.0 0.1944887950282112 0.0 44-45 0.0 0.0 0.0 0.25528009682659286 0.0 46-47 0.0 0.0 0.0 0.33217639510616287 0.0 48-49 0.0 0.0 0.0 0.41537590565305615 0.0 50-51 0.0 0.0 0.0 0.5064544315341035 0.0 52-53 0.0 0.0 0.0 0.6010604431794329 0.0 54-55 0.0 0.0 0.0 0.7063645673885677 0.0 56-57 0.0 0.0 0.0 0.8389922493620166 0.0 58-59 0.0 0.0 0.0 0.9843420111410723 0.0 60-61 0.0 0.0 0.0 1.140939248020994 0.0 62-63 0.0 0.0 0.0 1.3043312320698188 0.0 64-65 0.0 0.0 0.0 1.4767443272535283 0.0 66-67 0.0 0.0 0.0 1.6763364111128523 0.0 68-69 0.0 0.0 0.0 1.9048423127121912 0.0 70-71 0.0 0.0 0.0 2.161206677704035 0.0 72-73 0.0 0.0 0.0 2.419941975750559 0.0 74-75 0.0 0.0 0.0 2.6888260238238293 0.0 76-77 0.0 0.0 0.0 2.9855974491073436 0.0 78-79 0.0 0.0 0.0 3.3225879948672192 0.0 80-81 0.0 0.0 0.0 3.6886369274558097 0.0 82-83 0.0 0.0 0.0 4.064126221536515 2.8913817740015048E-5 84-85 0.0 0.0 0.0 4.443403225741163 2.8913817740015048E-5 86-87 0.0 0.0 0.0 4.84185009110744 2.8913817740015048E-5 88-89 0.0 0.0 0.0 5.279359524240478 2.8913817740015048E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7260 0.0 49.69073 1 GTATCAA 12725 0.0 39.294174 1 ATCAACG 15680 0.0 31.526285 3 TCAACGC 15835 0.0 31.24765 4 TATCAAC 16015 0.0 31.044558 2 CAACGCA 16350 0.0 30.292412 5 AACGCAG 16830 0.0 29.539928 6 ACGCAGA 19135 0.0 25.783964 7 CGCAGAG 19365 0.0 25.355238 8 GTACATG 13750 0.0 24.715736 1 TACATGG 13915 0.0 24.069807 2 GTGGTAT 4080 0.0 23.415646 1 ACATGGG 13895 0.0 23.250895 3 TGGTATC 3985 0.0 22.976301 2 GCAGAGT 21130 0.0 22.741722 9 GAGTACT 12525 0.0 21.430674 12-13 GTACTTT 13355 0.0 20.098925 14-15 CAGAGTA 20770 0.0 19.800457 10-11 CATGGGG 6490 0.0 18.859324 4 AGAGTAC 19680 0.0 18.859266 10-11 >>END_MODULE