FastQCFastQC Report
Thu 26 May 2016
SRR938684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458554
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140370.4058632596165912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128400.3712534197817932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85380.24686617586424847No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35980.10403191622857413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81500.048.3266071
GTATCAA135100.035.561461
ATCAACG171650.027.5654583
TCAACGC173400.027.2598884
TATCAAC174200.027.2164332
CAACGCA178450.026.4618595
AACGCAG185300.025.5794336
GTACATG137400.023.1953871
TGGTATC42500.023.1150742
GTGGTAT44750.022.853831
ACGCAGA210800.022.4623137
TACATGG140300.022.2577922
CGCAGAG213200.022.1204388
ACATGGG137300.021.3269373
GCAGAGT229450.020.2850239
GAGTACT138100.019.99742512-13
AGTACTT144550.019.03946312-13
TAGACCG3254.947651E-1018.9833935
GTACTTT146800.018.9739114-15
AGAGTAC214150.018.95610-11
Produced by FastQC (version 0.11.5)