##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938684_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3458554 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9530147570343 31.0 31.0 34.0 30.0 34.0 2 32.17324957193093 33.0 31.0 34.0 30.0 34.0 3 32.24408379918313 34.0 31.0 34.0 30.0 34.0 4 35.893877903887 37.0 35.0 37.0 35.0 37.0 5 35.797413022899164 37.0 35.0 37.0 35.0 37.0 6 35.82219852574226 37.0 35.0 37.0 35.0 37.0 7 35.74174351477525 37.0 35.0 37.0 33.0 37.0 8 35.72246291369168 37.0 35.0 37.0 33.0 37.0 9 37.352902687076735 39.0 37.0 39.0 34.0 39.0 10-11 37.270274368999296 39.0 37.0 39.0 33.5 39.0 12-13 37.1799653843774 39.0 37.0 39.0 33.0 39.0 14-15 38.360254169806225 40.0 38.0 41.0 33.0 41.0 16-17 38.27085944010126 40.0 38.0 41.0 33.0 41.0 18-19 38.427055208621866 40.0 38.0 41.0 33.5 41.0 20-21 38.43485080759184 40.0 38.0 41.0 34.0 41.0 22-23 38.35347778291158 40.0 38.0 41.0 34.0 41.0 24-25 38.27380662554351 40.0 38.0 41.0 33.0 41.0 26-27 38.0922074658947 40.0 37.0 41.0 33.0 41.0 28-29 37.90660750128522 40.0 37.0 41.0 33.0 41.0 30-31 37.66833812628052 40.0 37.0 41.0 32.0 41.0 32-33 37.510897184198946 40.0 36.0 41.0 32.0 41.0 34-35 37.48289675396134 40.0 36.0 41.0 32.0 41.0 36-37 37.3660667145865 40.0 36.0 41.0 31.5 41.0 38-39 37.239231193151824 40.0 36.0 41.0 31.0 41.0 40-41 37.05304167579861 40.0 36.0 41.0 31.0 41.0 42-43 36.852505555790074 39.0 36.0 41.0 30.5 41.0 44-45 36.59591407275988 39.0 35.0 41.0 30.0 41.0 46-47 36.28326491360262 39.0 35.0 41.0 30.0 41.0 48-49 35.981882168096845 39.0 35.0 40.5 28.5 41.0 50-51 35.74307803203304 39.0 35.0 40.0 28.0 41.0 52-53 35.50794609539131 38.5 35.0 40.0 27.5 41.0 54-55 35.21800960748335 38.0 34.0 40.0 27.0 41.0 56-57 34.93788661388545 38.0 34.0 40.0 26.0 41.0 58-59 34.617247265764824 38.0 34.0 40.0 26.0 41.0 60-61 34.245061230791826 37.0 33.0 40.0 25.5 41.0 62-63 33.86257753963072 37.0 33.0 40.0 24.5 41.0 64-65 33.442555038897765 36.0 32.5 39.0 23.0 40.5 66-67 33.01653277641465 36.0 32.0 39.0 22.5 40.0 68-69 32.64320695874635 35.0 31.5 38.5 22.0 40.0 70-71 32.19011948346043 35.0 31.0 38.0 20.5 40.0 72-73 31.672703534482906 35.0 31.0 37.0 19.0 39.5 74-75 31.14410372080355 34.5 30.0 37.0 18.0 39.0 76-77 29.251034247260563 32.5 28.0 35.0 13.0 37.0 78-79 30.2138434154852 34.0 29.5 36.0 13.0 38.0 80-81 30.258018090797485 34.0 30.0 35.5 12.0 37.0 82-83 29.899692328065427 34.0 30.0 35.0 7.5 37.0 84-85 29.458544380107988 34.0 29.0 35.0 4.5 36.0 86-87 28.806243447406054 34.0 29.0 35.0 2.0 36.0 88-89 28.148507150676267 33.0 27.5 35.0 2.0 35.5 90-91 27.570289635495065 33.0 26.5 35.0 2.0 35.0 92-93 27.193812067124007 33.0 26.0 35.0 2.0 35.0 94-95 26.764309014692266 33.0 25.0 35.0 2.0 35.0 96-97 26.038746684307952 32.0 24.5 34.0 2.0 35.0 98-99 25.044940457775127 32.0 20.0 34.0 2.0 35.0 100-101 21.225950209249298 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.029737871616255518 1101 2 0.0595033693718392 1101 3 0.03359775056056691 1101 4 0.07575899031363775 1101 5 -0.014731356981918964 1101 6 0.030539389734826727 1101 7 0.08185401630782962 1101 8 0.13042777095319025 1101 9 0.21071527550027014 1101 10-11 0.18704739886467792 1101 12-13 0.10807721876669518 1101 14-15 0.2686825827767976 1101 16-17 0.2212756643362539 1101 18-19 0.21702156796454375 1101 20-21 0.16707220427760205 1101 22-23 0.20249173428972966 1101 24-25 0.1531817529144135 1101 26-27 0.07407280934560134 1101 28-29 0.18555249924867923 1101 30-31 0.1803655175687382 1101 32-33 0.20310886175540332 1101 34-35 0.37547399391078073 1101 36-37 0.30266429400224126 1101 38-39 0.30888687943193105 1101 40-41 0.3847834450888641 1101 42-43 0.42411404611976167 1101 44-45 0.47248548974314986 1101 46-47 0.4738113848317269 1101 48-49 0.3722954033178283 1101 50-51 0.45911964406408856 1101 52-53 0.348788453179786 1101 54-55 0.35562248648354 1101 56-57 0.19541211054774266 1101 58-59 0.1932311294535154 1101 60-61 0.2697859906660227 1101 62-63 0.17417324142984114 1101 64-65 0.20415586665353658 1101 66-67 0.19659279847238054 1101 68-69 0.23215739641002386 1101 70-71 0.239533654952929 1101 72-73 0.18460113263769884 1101 74-75 0.23957989401847257 1101 76-77 0.17875814209923746 1101 78-79 0.22242288295795376 1101 80-81 0.40285316117281056 1101 82-83 0.39206707736266466 1101 84-85 0.3687787346386777 1101 86-87 0.4385569015539197 1101 88-89 0.4935768160068612 1101 90-91 0.3053620871442888 1101 92-93 0.376114854490492 1101 94-95 0.5367080167662195 1101 96-97 0.3030832603545157 1101 98-99 0.24548148433062167 1101 100-101 0.9372139803733788 1102 1 0.269387021093781 1102 2 0.19100862612881286 1102 3 0.18858062809493248 1102 4 0.11850242536829114 1102 5 0.005648377699870366 1102 6 0.048060392759261106 1102 7 0.17642291422912848 1102 8 0.17115923628419694 1102 9 0.24397918550330644 1102 10-11 0.23912509805336413 1102 12-13 0.3161342589804832 1102 14-15 0.31100511722175384 1102 16-17 0.35010741610850715 1102 18-19 0.3592730121725154 1102 20-21 0.38273513974030493 1102 22-23 0.2649769321582127 1102 24-25 0.28928375160222686 1102 26-27 0.277662046195708 1102 28-29 0.3181779973203973 1102 30-31 0.35204795984042647 1102 32-33 0.40301520826929504 1102 34-35 0.3847527700545399 1102 36-37 0.33494542248473635 1102 38-39 0.38828331251828985 1102 40-41 0.4131900156350241 1102 42-43 0.4325985878937857 1102 44-45 0.4384569496333768 1102 46-47 0.46461426044580634 1102 48-49 0.4241406690360989 1102 50-51 0.4126271891505837 1102 52-53 0.3207459350571682 1102 54-55 0.31598061805448197 1102 56-57 0.3012778352120975 1102 58-59 0.34157589109919684 1102 60-61 0.28070624318238657 1102 62-63 0.28753672577158085 1102 64-65 0.42054350647367045 1102 66-67 0.4262980760323387 1102 68-69 0.3864403731586705 1102 70-71 0.35517106674021903 1102 72-73 0.4760118157760722 1102 74-75 0.52008312648395 1102 76-77 0.49749776060430406 1102 78-79 0.4989445020711081 1102 80-81 0.6597315116552807 1102 82-83 0.6038790582879194 1102 84-85 0.8282973053128551 1102 86-87 0.8328618014595044 1102 88-89 0.8163102896270615 1102 90-91 0.937669619428938 1102 92-93 1.0593728836466916 1102 94-95 1.0665090281118523 1102 96-97 1.0689951116611063 1102 98-99 1.336695299119956 1102 100-101 2.1272628674131155 1103 1 0.16957543615600557 1103 2 0.22419234650266162 1103 3 0.1490427234869216 1103 4 -0.04072955969656533 1103 5 -0.011944741463629782 1103 6 0.04941538101273579 1103 7 0.11763451250185852 1103 8 0.08620224151228228 1103 9 0.1916615312002321 1103 10-11 0.20159386876666474 1103 12-13 0.18097304330328257 1103 14-15 0.19677530889211425 1103 16-17 0.1841694001144063 1103 18-19 -0.004243885960200089 1103 20-21 0.2197981944646017 1103 22-23 0.23208352158673762 1103 24-25 0.2691505197191333 1103 26-27 0.22198241455421908 1103 28-29 0.173082515124527 1103 30-31 0.29196995035692197 1103 32-33 0.3871556724041483 1103 34-35 0.31618341456427146 1103 36-37 0.3147575957293327 1103 38-39 0.24594345943811646 1103 40-41 0.3931296553559278 1103 42-43 0.3826349452548712 1103 44-45 0.3959747655247199 1103 46-47 0.47695968283048984 1103 48-49 0.4633887737076421 1103 50-51 0.44984343896918944 1103 52-53 0.4681962917672067 1103 54-55 0.5110713545753924 1103 56-57 0.3167315962575543 1103 58-59 0.5622837026370675 1103 60-61 0.7357436398855128 1103 62-63 0.7308147713379114 1103 64-65 0.6519362640245916 1103 66-67 0.6356027365989263 1103 68-69 0.6018313030364268 1103 70-71 0.6320106433161996 1103 72-73 0.7581705095780613 1103 74-75 0.763050271509421 1103 76-77 0.6496367265178087 1103 78-79 0.5786406612120238 1103 80-81 0.8567578560344025 1103 82-83 0.8232683958152265 1103 84-85 0.8387874041091763 1103 86-87 0.8191478597646338 1103 88-89 0.7827905284011898 1103 90-91 0.7028909992829568 1103 92-93 0.8400918730140248 1103 94-95 0.9607274232893062 1103 96-97 1.1254276975833157 1103 98-99 1.3506369155517568 1103 100-101 1.8979782092782038 1104 1 0.27381720864323356 1104 2 0.2952211108917524 1104 3 0.24396096641105203 1104 4 0.19121943313116674 1104 5 -0.04404688116969879 1104 6 0.08249336040323385 1104 7 0.13904817887274135 1104 8 0.13177598956149694 1104 9 0.1810694958046284 1104 10-11 0.24791019674671588 1104 12-13 0.19435585389933152 1104 14-15 0.22175039640740124 1104 16-17 0.31699862887127495 1104 18-19 0.37662165741107145 1104 20-21 0.3474968000982983 1104 22-23 0.2207905375131034 1104 24-25 0.21429818670840461 1104 26-27 0.33815005971124634 1104 28-29 0.36457563637858925 1104 30-31 0.1890954994567764 1104 32-33 0.36461012711192353 1104 34-35 0.3705712940382071 1104 36-37 0.44422708883062967 1104 38-39 0.43722366879810437 1104 40-41 0.4565834417807437 1104 42-43 0.44304703852571947 1104 44-45 0.4175386630270168 1104 46-47 0.28566813012353265 1104 48-49 0.42575953946229816 1104 50-51 0.4374568548976825 1104 52-53 0.43720794399624907 1104 54-55 0.3951589931961621 1104 56-57 0.38527133406797986 1104 58-59 0.5803560711637772 1104 60-61 0.6476449314054733 1104 62-63 0.6880850459529384 1104 64-65 0.5911262570564233 1104 66-67 0.4437549311038822 1104 68-69 0.47014132052934343 1104 70-71 0.42780509937426103 1104 72-73 0.5203260947692563 1104 74-75 0.6010685630657164 1104 76-77 0.5541620748832976 1104 78-79 0.46659275595789396 1104 80-81 0.6538898915017342 1104 82-83 0.7787923407241344 1104 84-85 0.796574288268733 1104 86-87 0.8986384101718947 1104 88-89 0.8773547497540903 1104 90-91 0.9440129397172115 1104 92-93 1.0444386435748356 1104 94-95 1.0277198662034266 1104 96-97 1.1857513829127875 1104 98-99 1.7468751498502648 1104 100-101 2.396309294991653 1105 1 0.07906642755989068 1105 2 0.04254263228885691 1105 3 0.029692535578028867 1105 4 -0.00108070618160383 1105 5 -0.07225875671695547 1105 6 -0.08890840022061042 1105 7 -0.04587993527593426 1105 8 0.010693939807822517 1105 9 0.04615728441270761 1105 10-11 0.0811925873507704 1105 12-13 0.0866166021221062 1105 14-15 0.06573033660071559 1105 16-17 -0.020368921957860664 1105 18-19 0.14514694433453457 1105 20-21 0.11679670144729926 1105 22-23 -0.0055701108264969434 1105 24-25 -0.032666735583717355 1105 26-27 -0.005690529206724193 1105 28-29 0.0603868964545029 1105 30-31 0.037084474572232295 1105 32-33 0.11607857149886769 1105 34-35 0.08321818275818771 1105 36-37 0.07557769879569065 1105 38-39 0.00767195711469526 1105 40-41 0.0394052685261741 1105 42-43 0.11971653919455605 1105 44-45 0.17031910409941275 1105 46-47 0.11674315648053124 1105 48-49 0.15810324385738994 1105 50-51 0.16147421470120804 1105 52-53 0.22646218556567277 1105 54-55 0.25484565883088806 1105 56-57 0.2689668497986162 1105 58-59 0.2055173513010473 1105 60-61 0.20409136221847035 1105 62-63 0.15021653903311716 1105 64-65 0.13932990013541513 1105 66-67 0.27795044028859195 1105 68-69 0.11946770593669953 1105 70-71 0.05205172071229214 1105 72-73 0.19913503604610838 1105 74-75 0.2220305142762129 1105 76-77 0.2683206496266415 1105 78-79 -0.020042189907275798 1105 80-81 0.22470971904346015 1105 82-83 0.3194382082792018 1105 84-85 0.25757495287304977 1105 86-87 0.416183964061446 1105 88-89 0.3408594247025114 1105 90-91 0.3809973996819238 1105 92-93 0.5534468429284054 1105 94-95 0.8349947805865234 1105 96-97 0.7344198254514716 1105 98-99 0.7706425706849522 1105 100-101 1.5201689364133664 1106 1 0.08371388371940114 1106 2 -0.011152919103274428 1106 3 0.07410964955756327 1106 4 0.07402264512086987 1106 5 -0.06441516871222319 1106 6 0.004406619146976709 1106 7 0.015659970395645928 1106 8 0.03789554285452823 1106 9 0.03508384847518897 1106 10-11 0.05939417890336074 1106 12-13 0.07323878654144522 1106 14-15 0.07853976790993045 1106 16-17 0.12296429643426876 1106 18-19 -0.04930082063368246 1106 20-21 -0.00472987104420497 1106 22-23 0.07041464796937902 1106 24-25 0.11760107742993142 1106 26-27 0.13206938159890314 1106 28-29 0.17176396874869226 1106 30-31 0.15919494839820203 1106 32-33 0.20176130311497076 1106 34-35 0.2149120713341759 1106 36-37 0.12790966432865503 1106 38-39 0.16621036471920547 1106 40-41 0.3548654356506944 1106 42-43 0.3397697569736664 1106 44-45 0.29260352228617137 1106 46-47 0.347956782377544 1106 48-49 0.3744603355383376 1106 50-51 0.4092761556750659 1106 52-53 0.269099434945943 1106 54-55 0.3747406408809013 1106 56-57 0.355056090152182 1106 58-59 0.4261690296342451 1106 60-61 0.35776066870153755 1106 62-63 0.5042208991362571 1106 64-65 0.5499468279101833 1106 66-67 0.4091560203010758 1106 68-69 0.3367596527725638 1106 70-71 0.5666080668079303 1106 72-73 0.5652352341281208 1106 74-75 0.7027838006369045 1106 76-77 0.6398951142879632 1106 78-79 0.5414722371299625 1106 80-81 0.5517912085282575 1106 82-83 0.5648689121408417 1106 84-85 0.5652480447532184 1106 86-87 0.6780640957074446 1106 88-89 0.5770682039119457 1106 90-91 0.5855971552402899 1106 92-93 0.8301041993572582 1106 94-95 1.115308416828256 1106 96-97 1.105784905546333 1106 98-99 1.3596511261421718 1106 100-101 1.9261613962701496 1107 1 0.06504899691323729 1107 2 0.03353861582415618 1107 3 -0.02304451841210664 1107 4 -0.008219356786561605 1107 5 0.020927586679867716 1107 6 -0.03024838507707983 1107 7 0.05230876066615764 1107 8 -0.013885640099260854 1107 9 -0.03682103789534352 1107 10-11 -0.042695640263431756 1107 12-13 0.030710155524886318 1107 14-15 0.07633050049989265 1107 16-17 0.05305191255693842 1107 18-19 0.058428620343867976 1107 20-21 0.04914778221593963 1107 22-23 0.025392500841498133 1107 24-25 0.03618704515906046 1107 26-27 0.007351529940315515 1107 28-29 0.08671320227292512 1107 30-31 0.16842949169439692 1107 32-33 0.16379364243355354 1107 34-35 0.1385070472733858 1107 36-37 0.005570781377819856 1107 38-39 0.0766943109686622 1107 40-41 -0.017511448255419282 1107 42-43 0.09274304973116188 1107 44-45 0.16429316066643906 1107 46-47 0.06299108047617352 1107 48-49 -0.0032054100816409914 1107 50-51 -0.060343383583777666 1107 52-53 -0.0970033628889766 1107 54-55 -0.04426567108944113 1107 56-57 -0.10282621034864547 1107 58-59 -0.02594576047757613 1107 60-61 0.01746114182704872 1107 62-63 0.06640376424492445 1107 64-65 -0.009800047610035278 1107 66-67 0.08061403566325964 1107 68-69 -0.04656496795690401 1107 70-71 0.031633029973839655 1107 72-73 0.08119804686781862 1107 74-75 0.0977139208863953 1107 76-77 0.12357648502948493 1107 78-79 0.1200460383301234 1107 80-81 0.25890108224217556 1107 82-83 0.24004969867115733 1107 84-85 0.24748697391197183 1107 86-87 0.27039540958366715 1107 88-89 0.2780463881741859 1107 90-91 0.5200908596060856 1107 92-93 0.6138921439139828 1107 94-95 0.6043128384747192 1107 96-97 0.7842973684594234 1107 98-99 1.1271465014609205 1107 100-101 1.814162857187121 1108 1 0.11900660321792955 1108 2 0.061975246345888024 1108 3 0.0022543644743393543 1108 4 0.0806654421199724 1108 5 0.025894858823868105 1108 6 0.06480977378652142 1108 7 0.02931220653341171 1108 8 0.05502759218180131 1108 9 0.1854622484488928 1108 10-11 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2631.0 10 4247.0 11 6412.0 12 8753.0 13 11949.0 14 14933.0 15 19554.0 16 24559.0 17 31495.0 18 30657.0 19 23344.0 20 22140.0 21 23504.0 22 25401.0 23 28689.0 24 33028.0 25 39548.0 26 47429.0 27 57431.0 28 69179.0 29 83962.0 30 100426.0 31 121273.0 32 147779.0 33 184919.0 34 242126.0 35 334407.0 36 483629.0 37 698721.0 38 498948.0 39 34995.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.952065711427515 20.110996996572315 15.06672222988567 22.8702150621145 2 16.074261092930744 21.13417919743338 39.28138753941676 23.510172170219114 3 19.511680314952436 24.79082298555986 30.151849588007014 25.545647111480697 4 13.445619180732757 17.666949829321734 36.38965879960237 32.497772190343134 5 13.534258397255753 37.560961643077334 34.377291077584076 14.527488882082837 6 31.733224767756024 36.69206160479662 17.20484890262408 14.369864724823273 7 28.051781177914236 32.83004978381138 20.822864121826637 18.29530491644774 8 25.85623934164393 35.96734357769172 19.530156244488303 18.646260836176044 9 25.98426972659672 16.846173285135926 20.28078786683683 36.888769121430514 10-11 24.785849808908576 27.044120172765844 27.244695326428324 20.92533469189725 12-13 25.723915254756758 24.29101873210596 27.82508238992365 22.159983623213634 14-15 22.529444386295545 25.708142767179577 26.269649107690668 25.492763738834206 16-17 21.93785282228188 29.00127412513953 26.89387143350138 22.167001619077208 18-19 21.702292345298062 27.277107716114884 29.51477698483239 21.50582295375466 20-21 22.520105223165523 26.417933621970334 29.70881761568563 21.35314353917851 22-23 22.156499508175962 26.55193471028644 29.45531571865005 21.83625006288755 24-25 22.00948491893841 26.674064772362232 29.646776113525263 21.669674195174096 26-27 21.875 26.931900192915336 29.413950276882083 21.779149530202584 28-29 21.69662523702102 26.97863037558471 29.599566755354985 21.725177632039287 30-31 21.87009368655224 26.736014530928244 29.601720834776614 21.7921709477429 32-33 21.609869326892106 26.90203767239141 29.454563959388807 22.033529041327675 34-35 22.171573001673067 26.93038928172597 29.201623448161797 21.696414268439167 36-37 22.126952789215522 26.712850789212922 29.189303378961736 21.97089304260982 38-39 21.9434981097887 26.95153870111122 29.34279407728973 21.76216911181034 40-41 22.107451040719063 26.769086500175522 29.346594638609574 21.77686782049584 42-43 21.982483481304353 26.882421412850356 29.09172873150615 22.04336637433914 44-45 22.105605266673336 27.076117864022535 28.8828068028635 21.93547006644063 46-47 22.12070547058479 27.18791224916276 28.469232342814927 22.222149937437518 48-49 22.292024541296406 27.30340665323694 28.314785295462165 22.08978351000449 50-51 22.133021488171067 27.446499317344763 28.227042284145337 22.19343691033883 52-53 22.40950987030996 27.378436190384768 28.06146730685714 22.150586632448128 54-55 22.1801943818139 27.292750091512236 28.249942027795434 22.277113498878435 56-57 22.189586199747783 27.665763630157613 28.01079595832491 22.133854211769698 58-59 22.288470443393553 27.350748863357577 28.072498190349894 22.28828250289898 60-61 22.216498765207923 27.28280396255346 28.382435389912956 22.11826188232566 62-63 22.228830679846443 27.24591741406973 28.32409193382971 22.201159972254118 64-65 22.040462569809787 27.2294821028161 28.24992301586957 22.480132311504537 66-67 21.966248233435863 27.535962004865056 28.02803370116422 22.469756060534866 68-69 22.03423081432525 27.332534250981734 28.19069722995226 22.442537704740758 70-71 21.89727088099297 27.287575840987788 28.129214191372576 22.685939086646666 72-73 22.069049666421282 27.221824496595975 28.069751115639658 22.63937472134308 74-75 21.945091514864743 27.35524115474855 27.939332142367157 22.760335188019557 76-77 21.976192866365352 27.2263352746375 28.123630341973683 22.673841517023465 78-79 21.986541413774074 27.271603315395115 28.002079505319795 22.73977576551102 80-81 21.828547768615877 27.393404010841614 27.98303381267048 22.795014407872024 82-83 21.95378615949951 27.34520812448985 27.865038653694302 22.835967062316342 84-85 21.90739251143686 27.2327539197017 27.800895981384127 23.05895758747731 86-87 21.914913507738966 27.11881944366057 27.981788391423684 22.984478657176787 88-89 21.88757085448408 27.10684782938259 27.850438194173744 23.155143121959583 90-91 21.825146086976208 27.211781345390452 27.91891690290096 23.044155664732386 92-93 21.82752601604319 27.047461370249117 27.938025275990814 23.18698733771688 94-95 21.652404394684233 26.963948970404296 27.874086826182825 23.50955980872865 96-97 21.809197496060488 26.773199751340883 27.88307238582643 23.534530366772202 98-99 21.531411682454575 26.756398772434952 27.836821399926098 23.875368145184375 100-101 21.76159353619841 26.159556018437936 28.447036046033535 23.631814399330118 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2707.0 1 1793.0 2 1121.5 3 1439.5 4 1204.5 5 1060.5 6 1932.0 7 4169.0 8 6219.0 9 6736.0 10 5410.5 11 3870.5 12 3651.0 13 3743.5 14 3990.5 15 4222.5 16 4482.5 17 4842.0 18 5212.5 19 5631.5 20 6282.0 21 7441.0 22 9029.5 23 10788.0 24 12967.5 25 15996.0 26 20547.0 27 25753.0 28 30386.0 29 35491.0 30 41606.5 31 47842.0 32 54241.0 33 60118.5 34 66563.5 35 72964.0 36 80307.5 37 88504.0 38 93793.0 39 96337.5 40 99141.5 41 105224.5 42 111287.0 43 116110.0 44 120827.5 45 125597.5 46 127593.5 47 129242.0 48 136103.5 49 138395.5 50 135192.5 51 131338.5 52 124015.5 53 119668.5 54 116020.5 55 106503.0 56 96055.0 57 86047.5 58 76273.0 59 66495.5 60 55643.5 61 44675.0 62 35310.5 63 27134.5 64 20157.0 65 15351.5 66 11582.0 67 8882.0 68 6523.0 69 4412.5 70 3165.0 71 2375.5 72 1835.0 73 1463.0 74 1181.0 75 978.0 76 686.0 77 389.0 78 235.5 79 157.5 80 84.0 81 51.5 82 35.5 83 23.0 84 17.5 85 12.0 86 6.5 87 3.5 88 3.0 89 2.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2263951929043178 2 0.0 3 0.0 4 0.0 5 0.024490003625792744 6 0.0035274857642818356 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0029636663183515424 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.1805199514016555E-4 26-27 0.001214380345080632 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006823660986643551 36-37 0.016871212651298782 38-39 0.013734063426507147 40-41 0.007864558425284092 42-43 0.007965756787374146 44-45 0.0037009686707219257 46-47 3.3250890401017306E-4 48-49 0.0016480876111808578 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0010842681652505643 58-59 3.614227217501881E-4 60-61 0.0022263639659811586 62-63 6.650178080203461E-4 64-65 0.0014167770692607373 66-67 0.0062598415407132575 68-69 1.0119836209005266E-4 70-71 5.782763548003009E-5 72-73 0.0 74-75 5.059918104502632E-4 76-77 0.05617954786884924 78-79 0.14017418840359294 80-81 8.095868967204213E-4 82-83 6.650178080203461E-4 84-85 0.0 86-87 0.10134293117875273 88-89 0.34596828616815006 90-91 0.38093954872469826 92-93 4.3370726610022565E-4 94-95 3.4696581288018056E-4 96-97 1.1565527096006018E-4 98-99 0.0 100-101 9.252421676804814E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3458554.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.82862152759178 #Duplication Level Percentage of deduplicated Percentage of total 1 73.28219310267959 39.44679437236036 2 15.180919511648622 16.34335941666734 3 5.236724408495864 8.456569686876774 4 2.296524675480133 4.944750303407824 5 1.1621274547523146 3.1277859464342943 6 0.696332669872416 2.248957664631588 7 0.4228236765418965 1.5932010960235043 8 0.2984452880016398 1.2851918763626717 9 0.22009365433969447 1.066260421806842 >10 1.0638620328060628 10.39687425528691 >50 0.08540436600401188 3.2154500083967625 >100 0.05062566664252681 5.060572121725833 >500 0.0031710420757804975 1.227975745248366 >1k 5.912112323841606E-4 0.5616661811038484 >5k 5.374647567128733E-5 0.24701276677988343 >10k+ 1.0749295134257466E-4 0.7775781368872016 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14037 0.4058632596165912 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12840 0.3712534197817932 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8538 0.24686617586424847 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3598 0.10403191622857413 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.7827635480030096E-5 0.0 5 0.0 0.0 0.0 5.7827635480030096E-5 0.0 6 0.0 0.0 0.0 5.7827635480030096E-5 0.0 7 0.0 0.0 0.0 5.7827635480030096E-5 0.0 8 0.0 0.0 0.0 5.7827635480030096E-5 0.0 9 0.0 0.0 0.0 5.7827635480030096E-5 0.0 10-11 0.0 0.0 0.0 8.674145322004514E-5 0.0 12-13 0.0 0.0 0.0 1.7348290644009028E-4 0.0 14-15 0.0 0.0 0.0 1.879398153100978E-4 0.0 16-17 0.0 0.0 0.0 2.3131054192012036E-4 0.0 18-19 0.0 0.0 0.0 2.746812685301429E-4 0.0 20-21 0.0 0.0 0.0 3.6142272175018807E-4 0.0 22-23 0.0 0.0 0.0 6.07190172540316E-4 0.0 24-25 0.0 0.0 0.0 8.385007144604364E-4 0.0 26-27 0.0 0.0 0.0 0.001185466527340617 0.0 28-29 0.0 0.0 0.0 0.0026889850498213992 0.0 30-31 0.0 0.0 0.0 0.007098342255173694 0.0 32-33 0.0 0.0 0.0 0.017536230459319124 0.0 34-35 0.0 0.0 0.0 0.0332364334921473 0.0 36-37 0.0 0.0 0.0 0.061485233424142 0.0 38-39 0.0 0.0 0.0 0.10251394079722334 0.0 40-41 0.0 0.0 0.0 0.14746047047407673 0.0 42-43 0.0 0.0 0.0 0.1964115639079222 0.0 44-45 0.0 0.0 0.0 0.25752091770144403 0.0 46-47 0.0 0.0 0.0 0.3352557166954745 0.0 48-49 0.0 0.0 0.0 0.41871545160202794 0.0 50-51 0.0 0.0 0.0 0.5102276847491756 0.0 52-53 0.0 0.0 0.0 0.6055420849291351 0.0 54-55 0.0 0.0 0.0 0.7114533993108103 0.0 56-57 0.0 0.0 0.0 0.8446304438213195 0.0 58-59 0.0 0.0 0.0 0.9903849990487354 0.0 60-61 0.0 0.0 0.0 1.146938865202047 0.0 62-63 0.0 0.0 0.0 1.310518789066182 0.0 64-65 0.0 0.0 0.0 1.483495703695822 0.0 66-67 0.0 0.0 0.0 1.6830010461019258 0.0 68-69 0.0 0.0 0.0 1.910263653538444 0.0 70-71 0.0 0.0 0.0 2.1668737859810774 0.0 72-73 0.0 0.0 0.0 2.4255657133009922 1.4456908870007524E-5 74-75 0.0 0.0 0.0 2.694478675192002 2.8913817740015048E-5 76-77 0.0 0.0 0.0 2.9909320484803765 2.8913817740015048E-5 78-79 0.0 0.0 0.0 3.328385215324092 2.8913817740015048E-5 80-81 0.0 0.0 0.0 3.6935811902893523 2.8913817740015048E-5 82-83 0.0 0.0 0.0 4.067928388569327 2.8913817740015048E-5 84-85 0.0 0.0 0.0 4.446164495335334 2.8913817740015048E-5 86-87 0.0 0.0 0.0 4.84270304873077 4.337072661002257E-5 88-89 0.0 0.0 0.0 5.2778126349913865 5.7827635480030096E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8150 0.0 48.326607 1 GTATCAA 13510 0.0 35.56146 1 ATCAACG 17165 0.0 27.565458 3 TCAACGC 17340 0.0 27.259888 4 TATCAAC 17420 0.0 27.216433 2 CAACGCA 17845 0.0 26.461859 5 AACGCAG 18530 0.0 25.579433 6 GTACATG 13740 0.0 23.195387 1 TGGTATC 4250 0.0 23.115074 2 GTGGTAT 4475 0.0 22.85383 1 ACGCAGA 21080 0.0 22.462313 7 TACATGG 14030 0.0 22.257792 2 CGCAGAG 21320 0.0 22.120438 8 ACATGGG 13730 0.0 21.326937 3 GCAGAGT 22945 0.0 20.285023 9 GAGTACT 13810 0.0 19.997425 12-13 AGTACTT 14455 0.0 19.039463 12-13 TAGACCG 325 4.947651E-10 18.983393 5 GTACTTT 14680 0.0 18.97391 14-15 AGAGTAC 21415 0.0 18.956 10-11 >>END_MODULE