FastQCFastQC Report
Thu 26 May 2016
SRR938683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920323
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120650.6282797216926528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93260.4856474665980671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64880.33785982878921933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29350.15283887137736724No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.12628083921298658No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.104878189762868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67650.036.3193471
GTATCAA106700.034.318551
TATCAAC129400.028.0292032
ATCAACG129350.027.966733
TCAACGC129250.027.9150034
CAACGCA131750.027.457285
GTATACG700.00845135927.1042881
AACGCAG135300.026.7537696
GTACATG88800.024.3038181
ACGCAGA151750.023.7611797
CGCAGAG152500.023.5200398
TACATGG90350.023.2990632
ACATGGG88200.022.7919183
GAGTACT87050.022.5070612-13
GCAGAGT161400.021.9001629
GTACTTT92750.021.04779614-15
GTACCTG17400.020.7176461
TACCTGG15800.020.7050172
AGTACTT91700.020.43568212-13
CATGGGG35750.020.158174
Produced by FastQC (version 0.11.5)