##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938683_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1920323 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.327415752454144 33.0 31.0 34.0 31.0 34.0 2 32.448045459019134 34.0 31.0 34.0 31.0 34.0 3 32.47418949832919 34.0 31.0 34.0 31.0 34.0 4 35.98632261343534 37.0 35.0 37.0 35.0 37.0 5 36.00269277616318 37.0 37.0 37.0 35.0 37.0 6 36.101176208377446 37.0 37.0 37.0 35.0 37.0 7 36.05032851244296 37.0 37.0 37.0 35.0 37.0 8 36.03826075092576 37.0 37.0 37.0 35.0 37.0 9 37.75570932598318 39.0 38.0 39.0 35.0 39.0 10-11 37.67769458575458 39.0 38.0 39.0 35.0 39.0 12-13 37.593293940654775 39.0 38.0 39.0 35.0 39.0 14-15 38.928245144176266 40.0 38.0 41.0 36.0 41.0 16-17 38.865082072130576 40.0 38.0 41.0 36.0 41.0 18-19 38.818173817633806 40.0 38.5 41.0 35.5 41.0 20-21 38.73619724390116 40.0 38.0 41.0 35.0 41.0 22-23 38.670416903822954 40.0 38.0 41.0 35.0 41.0 24-25 38.70394824204053 40.0 38.0 41.0 35.0 41.0 26-27 38.63013591984265 40.0 38.0 41.0 35.0 41.0 28-29 38.52022602447609 40.0 38.0 41.0 35.0 41.0 30-31 38.3222098574042 40.0 38.0 41.0 34.5 41.0 32-33 38.11823375546717 40.0 38.0 41.0 34.0 41.0 34-35 37.840458089602635 40.0 38.0 41.0 33.5 41.0 36-37 37.61473590640742 40.0 37.0 41.0 33.0 41.0 38-39 37.46653687947288 40.0 37.0 41.0 33.0 41.0 40-41 37.296128047208725 40.0 37.0 41.0 33.0 41.0 42-43 37.0528746986835 40.0 37.0 41.0 32.0 41.0 44-45 37.00567326434147 40.0 37.0 41.0 32.0 41.0 46-47 36.806911649758916 40.0 37.0 41.0 31.0 41.0 48-49 36.56862283063839 40.0 37.0 41.0 31.0 41.0 50-51 35.46777260908712 38.5 35.0 40.0 29.0 40.5 52-53 35.57922859852223 38.5 35.5 40.0 29.5 40.5 54-55 36.094007883048846 39.0 36.0 41.0 30.0 41.0 56-57 36.00676084179589 39.0 35.5 41.0 30.0 41.0 58-59 35.80020861073892 39.0 35.0 41.0 29.0 41.0 60-61 35.540824121775344 39.0 35.0 41.0 28.5 41.0 62-63 35.20674256362081 38.0 35.0 40.0 28.0 41.0 64-65 34.834755403127495 38.0 35.0 40.0 27.5 41.0 66-67 34.456846842952984 37.0 34.0 40.0 26.5 41.0 68-69 34.06878165808564 36.5 34.0 39.0 26.0 41.0 70-71 33.67321148577609 36.0 34.0 39.0 26.0 41.0 72-73 33.26910082314278 35.5 34.0 39.0 25.5 40.0 74-75 32.83084460270486 35.0 33.0 37.5 24.5 40.0 76-77 32.40598534725669 35.0 33.0 37.0 23.5 39.0 78-79 32.003809775751265 35.0 33.0 36.5 22.0 39.0 80-81 31.589839573863358 35.0 32.0 36.0 20.0 38.5 82-83 31.16096276511816 35.0 32.0 36.0 18.5 37.0 84-85 30.79057741848637 35.0 31.5 35.0 17.5 37.0 86-87 30.454728709701442 34.0 31.0 35.0 11.5 36.5 88-89 30.142285438439263 34.0 31.0 35.0 7.0 36.0 90-91 29.801093878477737 34.0 31.0 35.0 2.0 36.0 92-93 29.42788661074205 34.0 30.5 35.0 2.0 35.5 94-95 29.008620164420257 34.0 30.0 35.0 2.0 35.0 96-97 28.536819587121542 34.0 29.0 35.0 2.0 35.0 98-99 27.969216376619976 34.0 29.0 35.0 2.0 35.0 100-101 25.770817721810342 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2245717888833454 1101 2 0.1163095650311945 1101 3 0.18214067852963467 1101 4 0.15784702911148685 1101 5 0.04799732813329172 1101 6 0.04176402577392224 1101 7 0.13134290408179794 1101 8 0.208083083405306 1101 9 0.23338925795918897 1101 10-11 0.2478790754013147 1101 12-13 0.27799781381450117 1101 14-15 0.3779517596966997 1101 16-17 0.26004801231754016 1101 18-19 0.3491199476510545 1101 20-21 0.3685498993503913 1101 22-23 0.3469872187893728 1101 24-25 0.4362300990226089 1101 26-27 0.2695361917442227 1101 28-29 0.28116754627523477 1101 30-31 0.29194643134225373 1101 32-33 0.2214576966172075 1101 34-35 0.23712123870521395 1101 36-37 0.33935398367231073 1101 38-39 0.32439366334258324 1101 40-41 0.40619730099530216 1101 42-43 0.3540140943346799 1101 44-45 0.5302329767342115 1101 46-47 0.5474802000949737 1101 48-49 0.5573168768986392 1101 50-51 0.5016671101506844 1101 52-53 0.4259792379061338 1101 54-55 0.481416822963908 1101 56-57 0.5040590585104923 1101 58-59 0.43391852211342297 1101 60-61 0.30740144218759013 1101 62-63 0.25108546986761837 1101 64-65 0.29826895542321097 1101 66-67 0.299712886798126 1101 68-69 0.1733676003202973 1101 70-71 0.08730566918618621 1101 72-73 0.07069757389082554 1101 74-75 0.08759768983530591 1101 76-77 0.10258102715553719 1101 78-79 -0.104233074288139 1101 80-81 -0.1910869080014237 1101 82-83 -0.20276529756523942 1101 84-85 -0.11414336818329573 1101 86-87 -0.04015748389542395 1101 88-89 -0.1444533565809749 1101 90-91 -0.294856659137011 1101 92-93 -0.41890438110528905 1101 94-95 -0.5220615748102944 1101 96-97 -0.6356652467096389 1101 98-99 -0.8009386328631649 1101 100-101 -0.43680522327035476 1102 1 0.07437956783792288 1102 2 0.08701753532909606 1102 3 0.06006644668237726 1102 4 0.08840590375528024 1102 5 0.006718897265578505 1102 6 0.04024100313455392 1102 7 0.13776174115909612 1102 8 0.09056668463879447 1102 9 0.11792134453765613 1102 10-11 0.11091786484757904 1102 12-13 0.11526962080935022 1102 14-15 0.06974997733656352 1102 16-17 0.14166559483800967 1102 18-19 0.11620234230981907 1102 20-21 0.07393173753361992 1102 22-23 0.07402759983759921 1102 24-25 0.12160947958516033 1102 26-27 0.016412263296096796 1102 28-29 0.004969017336755144 1102 30-31 0.033143877757353835 1102 32-33 -0.04782087468597496 1102 34-35 -0.06582579956931056 1102 36-37 -0.056337456200829195 1102 38-39 -0.07904688856880426 1102 40-41 -0.03174801109326353 1102 42-43 -0.05393221250511715 1102 44-45 0.11914056434546438 1102 46-47 0.03500543459294647 1102 48-49 -0.09708337588519811 1102 50-51 -0.01313724568938568 1102 52-53 -0.01847549213594135 1102 54-55 -0.06069684784365137 1102 56-57 -0.12023709648345005 1102 58-59 -0.18779853552948822 1102 60-61 -0.12306135119087713 1102 62-63 -0.17443867625549103 1102 64-65 -0.10644405884854535 1102 66-67 -0.12603934992925048 1102 68-69 -0.21257878844019018 1102 70-71 -0.341661486468972 1102 72-73 -0.44924145371059154 1102 74-75 -0.3351223111009247 1102 76-77 -0.3503323651216874 1102 78-79 -0.44970607849535327 1102 80-81 -0.402864294380354 1102 82-83 -0.3728346375770606 1102 84-85 -0.5102203034132877 1102 86-87 -0.3513655214853202 1102 88-89 -0.41711770996342423 1102 90-91 -0.3012372275495636 1102 92-93 -0.5131231210480607 1102 94-95 -0.38813938776195656 1102 96-97 -0.3819060818325468 1102 98-99 -0.6115945250087265 1102 100-101 -0.518552123449993 1103 1 0.06438921580478763 1103 2 0.1675971192899368 1103 3 0.16118561083900573 1103 4 0.21470108447817182 1103 5 0.18884144997642238 1103 6 0.20264546473094214 1103 7 0.07169599379464131 1103 8 0.05556574337373377 1103 9 0.16001142360391896 1103 10-11 0.10364200359005338 1103 12-13 0.16954661158495554 1103 14-15 0.30793545361668606 1103 16-17 0.2608438704404179 1103 18-19 0.35502034871042554 1103 20-21 0.2808625074884432 1103 22-23 0.29201905785718196 1103 24-25 0.21980601572974479 1103 26-27 0.21962633050242175 1103 28-29 0.21630949644066533 1103 30-31 0.15036526329951982 1103 32-33 0.06892423073571052 1103 34-35 0.16669479060388426 1103 36-37 0.18352076574947063 1103 38-39 0.0083240404598115 1103 40-41 0.17095565513414357 1103 42-43 0.20384851979910223 1103 44-45 0.3892931669433537 1103 46-47 0.4074422521757768 1103 48-49 0.4104872189943194 1103 50-51 0.4381725246419421 1103 52-53 0.3550923730749034 1103 54-55 0.2632083432331527 1103 56-57 0.28210653585841783 1103 58-59 0.2468821402988297 1103 60-61 0.17340831654255595 1103 62-63 0.27319816015264564 1103 64-65 0.07767491705507013 1103 66-67 0.11817281121532375 1103 68-69 0.011326167320355296 1103 70-71 0.0657350920325328 1103 72-73 0.14769672870657757 1103 74-75 0.11253311529214471 1103 76-77 0.028949684953616384 1103 78-79 0.12617223946398326 1103 80-81 0.07230478035609167 1103 82-83 -0.07552736159814444 1103 84-85 0.13413104730207337 1103 86-87 0.08735482866528343 1103 88-89 -0.10253551399203786 1103 90-91 -0.16384053138992272 1103 92-93 -0.25069018618360417 1103 94-95 -0.38986778572882486 1103 96-97 -0.2714395673370653 1103 98-99 -0.35206409861565646 1103 100-101 -0.22072938719246338 1104 1 0.18825752355104441 1104 2 0.12909547301499913 1104 3 0.16108137923052368 1104 4 0.19029335380485435 1104 5 0.11293648582429938 1104 6 0.11422832398904603 1104 7 0.1423394535047393 1104 8 0.08887212585503335 1104 9 0.2676217922136459 1104 10-11 0.17834988720133538 1104 12-13 0.2281753083976099 1104 14-15 0.2296976983953627 1104 16-17 0.27229083895662853 1104 18-19 0.1604796009704046 1104 20-21 0.26007000631948785 1104 22-23 0.2999212993238274 1104 24-25 0.25054557308635594 1104 26-27 0.23813145928203738 1104 28-29 0.1884320465108047 1104 30-31 0.32317542539428246 1104 32-33 0.20286866652124758 1104 34-35 0.18937234963598826 1104 36-37 0.27941713322920947 1104 38-39 0.21624259855675376 1104 40-41 0.005660275419366201 1104 42-43 0.22578415921618245 1104 44-45 0.13334721639004954 1104 46-47 0.16851503408818047 1104 48-49 0.09240254785965618 1104 50-51 0.182397411643187 1104 52-53 0.21681940315595227 1104 54-55 0.3021099904822151 1104 56-57 0.20266829065112546 1104 58-59 0.2444046913430924 1104 60-61 0.2049499458676749 1104 62-63 0.23088536304385343 1104 64-65 0.29527934348898555 1104 66-67 0.31756008521781354 1104 68-69 0.25015353737179424 1104 70-71 0.3214007374892063 1104 72-73 0.2553038096370628 1104 74-75 0.2012752492151506 1104 76-77 0.11055938141558386 1104 78-79 0.2640727275016985 1104 80-81 0.22174526605590472 1104 82-83 0.07526679558701588 1104 84-85 0.11959792210724984 1104 86-87 0.025081313235109093 1104 88-89 0.16415140640228998 1104 90-91 0.20645078134386807 1104 92-93 0.34569068780330525 1104 94-95 0.12491532485409351 1104 96-97 0.09938106175426853 1104 98-99 0.19323196298814693 1104 100-101 0.20174652751295952 1105 1 0.06272845982346809 1105 2 0.09922924809622913 1105 3 0.08624518415016524 1105 4 0.07223690842904062 1105 5 0.054795502560864406 1105 6 0.12783198117157468 1105 7 0.002680412774935803 1105 8 0.10585321458663088 1105 9 0.08771281338130166 1105 10-11 0.14804935909478445 1105 12-13 0.1532026836646665 1105 14-15 0.2270581866322985 1105 16-17 0.11945721166355128 1105 18-19 0.0948314133534609 1105 20-21 0.1872529445726272 1105 22-23 0.3059360730343812 1105 24-25 0.19419349014120968 1105 26-27 0.14026213566171464 1105 28-29 0.2641593097243131 1105 30-31 0.13224538100678274 1105 32-33 0.09529241778790976 1105 34-35 0.060467130595569074 1105 36-37 0.10589929017053379 1105 38-39 0.11018915488854475 1105 40-41 0.15683173265141903 1105 42-43 0.0638817249232062 1105 44-45 0.07188661507858995 1105 46-47 0.06838090166080946 1105 48-49 0.14321295786331234 1105 50-51 0.24520597365678043 1105 52-53 0.16027665051860396 1105 54-55 0.09800510428420495 1105 56-57 0.044969523240069975 1105 58-59 0.09608919553808448 1105 60-61 0.15371289009360822 1105 62-63 0.07948469378558087 1105 64-65 0.07548389863132599 1105 66-67 0.14430310484377884 1105 68-69 0.02095810221351968 1105 70-71 0.03687127302345061 1105 72-73 0.01863179979473273 1105 74-75 0.032378274660750606 1105 76-77 0.09691973437148249 1105 78-79 0.05401381643750369 1105 80-81 0.09665612095550458 1105 82-83 0.14832085440108855 1105 84-85 0.04592131526202792 1105 86-87 0.1882666201826808 1105 88-89 0.17952137452190797 1105 90-91 0.0967972029051154 1105 92-93 0.16145667202391678 1105 94-95 0.37299994952578075 1105 96-97 0.37729604910676784 1105 98-99 0.35547617334250603 1105 100-101 0.4161280506753684 1106 1 0.16861342231588594 1106 2 0.13355690429320077 1106 3 0.18618051129592317 1106 4 0.2016136171152496 1106 5 0.1424488667518773 1106 6 0.1730655650899493 1106 7 0.18728453206161078 1106 8 0.20631741602386455 1106 9 0.1834484136496144 1106 10-11 0.21563396161062087 1106 12-13 0.18397640617536126 1106 14-15 0.20101613858731326 1106 16-17 0.21659717494433295 1106 18-19 0.319478318276353 1106 20-21 0.2664580831248671 1106 22-23 0.21906078741820778 1106 24-25 0.254788881264993 1106 26-27 0.062272151936824116 1106 28-29 0.20884771751605768 1106 30-31 0.17948569410891935 1106 32-33 0.19653723661038214 1106 34-35 0.2682105022961139 1106 36-37 0.3380681558033203 1106 38-39 0.35350713955413227 1106 40-41 0.14830106248564334 1106 42-43 0.19364713884284157 1106 44-45 0.18253104532944775 1106 46-47 0.164956046439805 1106 48-49 0.2920034151431423 1106 50-51 0.41862342624115456 1106 52-53 0.3271239290474881 1106 54-55 0.3088177641290386 1106 56-57 0.3652761313626627 1106 58-59 0.24753003724726597 1106 60-61 0.32875531495150057 1106 62-63 0.1159227695276428 1106 64-65 0.06746767162426437 1106 66-67 0.09392856349992229 1106 68-69 0.3232625403213305 1106 70-71 0.2972073046222832 1106 72-73 0.27036407021277853 1106 74-75 0.13553294700722773 1106 76-77 -0.011174794892994555 1106 78-79 0.3047530111268415 1106 80-81 0.2673688051194745 1106 82-83 0.3191902767014625 1106 84-85 0.29851290618751847 1106 86-87 0.5558269035673717 1106 88-89 0.5628608989893564 1106 90-91 0.5574447064527668 1106 92-93 0.6918188400076488 1106 94-95 0.6805510397444152 1106 96-97 0.6288504691756636 1106 98-99 0.5395307069897619 1106 100-101 0.7635202915363237 1107 1 0.1072351964951821 1107 2 0.11096640733978091 1107 3 0.11127772594832663 1107 4 0.07405168312470778 1107 5 0.11756001289938212 1107 6 0.13891777163490104 1107 7 0.03197948668571371 1107 8 0.1363372735012689 1107 9 0.09206432980511892 1107 10-11 0.10797286255836269 1107 12-13 0.16832895862913944 1107 14-15 0.18194643625918872 1107 16-17 0.1948087243576495 1107 18-19 0.25066719808900473 1107 20-21 0.19181612254896407 1107 22-23 0.17679548553970648 1107 24-25 0.1542680472237521 1107 26-27 0.20199444627057517 1107 28-29 0.1444044268561342 1107 30-31 0.15715697334637468 1107 32-33 0.14301284018391414 1107 34-35 0.13593319894426514 1107 36-37 0.3283650393618487 1107 38-39 0.2796318869183594 1107 40-41 0.22265487896775937 1107 42-43 0.2761713354712825 1107 44-45 0.16139227812404755 1107 46-47 0.35068000167961344 1107 48-49 0.3895498410010845 1107 50-51 0.6152872069363156 1107 52-53 0.4071287676935853 1107 54-55 0.30762223797384536 1107 56-57 0.3839459658021269 1107 58-59 0.36194036802852736 1107 60-61 0.3391238811492059 1107 62-63 0.25085249362967943 1107 64-65 0.219845336107646 1107 66-67 0.1984359798124089 1107 68-69 0.13568372543624463 1107 70-71 0.29757197000136415 1107 72-73 0.1289205226696808 1107 74-75 0.347516751134485 1107 76-77 0.32957054689075704 1107 78-79 0.24861683314077965 1107 80-81 0.3328737436645923 1107 82-83 0.3033628750136437 1107 84-85 0.2482535085445896 1107 86-87 0.21636325933290124 1107 88-89 0.3087428169960553 1107 90-91 0.22375590479240515 1107 92-93 0.2541999975585867 1107 94-95 0.30544954370571276 1107 96-97 0.24415200789230695 1107 98-99 0.3898576553943016 1107 100-101 0.3930758564550487 1108 1 0.09230827749519932 1108 2 0.05713676873941864 1108 3 -0.04217214499001187 1108 4 -0.019949174589186214 1108 5 0.05279667989870518 1108 6 0.08550350391487882 1108 7 0.0321796199280584 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8 1764.0 9 2222.0 10 2529.0 11 3570.0 12 5198.0 13 7467.0 14 9373.0 15 8365.0 16 11802.0 17 16751.0 18 14159.0 19 8675.0 20 7907.0 21 8050.0 22 8376.0 23 9090.0 24 9941.0 25 11340.0 26 13300.0 27 15701.0 28 18610.0 29 23526.0 30 28772.0 31 37613.0 32 50535.0 33 70146.0 34 105350.0 35 170241.0 36 272027.0 37 440564.0 38 435529.0 39 79854.0 40 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19719679529462 19.01142191202964 14.590795205977667 23.200586086698074 2 14.96761182129438 20.257020912914104 41.22580006357776 23.549567202213755 3 19.113769434560844 24.906097121214962 29.966534723221006 26.013598721003188 4 12.422527929554358 16.64083395700131 37.47709453416693 33.45954357927741 5 13.313883512706411 37.37164708218809 34.32542361219344 14.989045792912057 6 31.081276060453746 36.20546771433706 17.857814424827392 14.855441800381808 7 27.72001671019582 33.16932199734762 21.019113588702073 18.091547703754483 8 25.77639816262766 35.021862813838425 19.90906903064961 19.2926699928843 9 26.28455666978693 16.925273570108804 20.768147672381595 36.022022087722675 10-11 24.50082916234418 26.7995896251957 27.35667941252539 21.34290179993473 12-13 25.64716521489086 24.393063252590757 27.959058385584324 22.000713146934054 14-15 22.590245103082378 25.478739544278344 26.93633518735325 24.994680165286027 16-17 21.50328823138731 28.92638268501999 27.34094727109352 22.229381812499184 18-19 21.155510107763707 27.212380506370128 30.257498321319943 21.374611064546226 20-21 22.11922403109377 26.219581759448392 30.362083399101795 21.299110810356044 22-23 21.67925508844457 26.354854129301913 30.237662630296352 21.72822815195716 24-25 21.48996783820363 26.476466136324856 30.397209257491063 21.63635676798045 26-27 21.336198474229104 26.683196459286112 30.33150944605481 21.64909562042997 28-29 21.00078335178174 26.92052092502722 30.43958617559949 21.639109547591552 30-31 21.280274653427178 26.653390709087216 30.3765023365338 21.689832300951807 32-33 21.06692011604217 26.82688535965206 30.321329911176377 21.784864613129393 34-35 21.644448040254513 26.882911144633344 29.979747469738648 21.4928933453735 36-37 21.532023459904718 26.68194349958824 30.056820305294472 21.72921273521257 38-39 21.279892594525258 26.98808176550101 30.156025892913835 21.5759997470599 40-41 21.50442799232041 26.85239877559135 30.08978770350752 21.553385528580726 42-43 21.287469197582322 26.91406158131708 29.823900324290992 21.974568896809615 44-45 21.374605973779843 27.087888165806966 29.68557347899738 21.85193238141581 46-47 21.403750117927824 27.236950387731014 29.319666859445448 22.03963263489571 48-49 21.580527410511305 27.32980067506287 29.12963726740153 21.960034647024294 50-51 21.43854173717535 27.565773328758052 29.014565013599082 21.981119920467513 52-53 21.601575904983612 27.629093606341048 28.82307110677948 21.946259381895857 54-55 21.38564418640145 27.403925561518793 29.002761859164 22.207668392915757 56-57 21.529636971078194 27.73528524895495 28.798785914475744 21.936291865491114 58-59 21.62788024452688 27.4778462824599 28.865536339411673 22.02873713360155 60-61 21.567007099704316 27.3526275278805 29.27597015319095 21.804395219224233 62-63 21.473777400567766 27.489307350438786 29.199086055447083 21.837829193546366 64-65 21.306287455593058 27.51093460877848 29.119086012300503 22.06369192332796 66-67 21.315121674776925 27.666765648754392 28.738699835424853 22.27941284104383 68-69 21.224911582583527 27.58788086019448 28.96248445555288 22.224723101669113 70-71 21.395990088281632 27.472969288469013 28.894766024001335 22.23627459924802 72-73 21.40203847608582 27.42851571515913 28.919249047429478 22.250196761325572 74-75 21.190668551015825 27.795727071871433 28.722724398775608 22.29087997833713 76-77 21.37505145074775 27.610424738172863 28.642497337627976 22.372026473451413 78-79 21.377582467426766 27.574563680548607 28.72255342475202 22.3253004272726 80-81 21.33802090069752 27.800984807212686 28.56445209157218 22.296542200517617 82-83 21.32005641280122 27.733576252269852 28.58123163768285 22.365135697246075 84-85 21.38586912496152 27.58544548406434 28.44621103755436 22.58247435341978 86-87 21.313692469800174 27.593641415471843 28.671986393875155 22.420679720852828 88-89 21.43512232477846 27.424273114685114 28.519073674526314 22.621530886010113 90-91 21.34383426016245 27.456593764473535 28.66264779343577 22.536924181928246 92-93 21.47967991803225 27.413742405371817 28.629065878702892 22.477511797893044 94-95 21.34413624698687 27.303582714850233 28.58934098811268 22.76294005005021 96-97 21.347404740277987 27.28120813701978 28.611465800599888 22.759921322102347 98-99 21.371239127838095 27.17869930560874 28.402502809112846 23.047558757440324 100-101 21.533460974958302 26.79982792947199 28.791235902317048 22.87547519325266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3743.0 1 2316.5 2 1210.0 3 1541.5 4 1216.5 5 1072.5 6 2028.0 7 4190.0 8 6352.5 9 6755.0 10 5154.5 11 3599.5 12 3375.0 13 3462.0 14 3613.5 15 3766.5 16 3838.5 17 3907.5 18 4003.5 19 4256.0 20 4621.0 21 5217.0 22 6072.0 23 7062.0 24 8304.0 25 9994.0 26 12548.5 27 15556.5 28 18231.5 29 21067.5 30 24409.5 31 28289.5 32 32133.0 33 35833.0 34 40116.5 35 43323.5 36 46745.0 37 50646.0 38 52558.5 39 53568.0 40 55031.5 41 57566.5 42 59985.5 43 62578.5 44 64992.0 45 66155.5 46 66862.5 47 68641.5 48 74359.5 49 74750.0 50 69165.0 51 66495.5 52 63950.0 53 62096.0 54 60372.5 55 55882.5 56 50307.0 57 44990.0 58 40322.5 59 35607.5 60 30258.0 61 24608.5 62 19602.5 63 15494.0 64 11802.0 65 8932.0 66 6667.0 67 5246.5 68 3863.5 69 2438.0 70 1751.5 71 1357.5 72 1048.5 73 870.0 74 692.0 75 548.5 76 386.5 77 237.0 78 147.5 79 96.0 80 75.0 81 53.5 82 43.5 83 37.0 84 28.5 85 21.0 86 15.5 87 9.0 88 6.5 89 8.0 90 10.5 91 7.5 92 4.0 93 3.5 94 2.5 95 3.0 96 3.0 97 2.0 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1311237744900207 2 0.07358137146719589 3 0.06868636161729043 4 0.08160085568938141 5 0.09800434614385184 6 0.026453883018638012 7 0.027964045631906718 8 0.03254660804458417 9 0.031973787742999485 10-11 0.03199982502943515 12-13 0.03449940452725921 14-15 0.03231227246666316 16-17 0.03114059457705813 18-19 0.03436921809508088 20-21 0.03697294673864761 22-23 0.04689315287063687 24-25 0.05834955890233049 26-27 0.060510653676490875 28-29 0.05241305759499834 30-31 0.04233662774439508 32-33 0.035202411261022235 34-35 0.23501254736833338 36-37 0.37423391793984656 38-39 0.35548707170616606 40-41 0.3342927205475329 42-43 0.3602518951238932 44-45 0.36775063361736543 46-47 0.3685057149239998 48-49 0.38097757512668445 50-51 0.37194263673350786 52-53 0.37824366005093935 54-55 0.3707188842710315 56-57 0.35327390235913436 58-59 0.33447498155258254 60-61 0.30971352215226294 62-63 0.35717949532448445 64-65 0.389231394926791 66-67 0.3915487134195653 68-69 0.3990214146266019 70-71 0.3977195503048185 72-73 0.3891272457810483 74-75 0.38391978849391484 76-77 0.3689743860798418 78-79 0.3475196620568519 80-81 0.35152940416794465 82-83 0.3607466035661709 84-85 0.35983529854092255 86-87 0.3680110064817221 88-89 0.37912892778975205 90-91 0.3826960360314385 92-93 0.3840760122125288 94-95 0.38764312045421523 96-97 0.38850235090659224 98-99 0.3828782970364881 100-101 0.3725414943215282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1920323.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.88841051588671 #Duplication Level Percentage of deduplicated Percentage of total 1 77.41677812852305 45.58951011249785 2 13.28685617895009 15.648836822631177 3 4.282290673913488 7.565318734613119 4 1.7363555648067555 4.090048772075381 5 0.9525708998227778 2.8047693097125665 6 0.531040256275685 1.876326996721453 7 0.345309397097852 1.4234305071904154 8 0.24339815745865354 1.1466664492188499 9 0.17608757338155995 0.9332565577235665 >10 0.9211775389416624 9.81270515502239 >50 0.07298462091216856 2.9485606662237744 >100 0.03276117879133362 3.3889046483614584 >500 0.0012391716943732027 0.4355957996190458 >1k 8.851226388379987E-4 0.8829193789334684 >5k 1.7702452776759974E-4 0.8242804804565718 >10k+ 8.851226388379987E-5 0.6288696089988958 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12065 0.6282797216926528 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9326 0.4856474665980671 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6488 0.33785982878921933 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2935 0.15283887137736724 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2425 0.12628083921298658 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2014 0.104878189762868 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 5.207457287133467E-5 3 0.0 0.0 0.0 0.0 5.207457287133467E-5 4 0.0 0.0 0.0 0.0 5.207457287133467E-5 5 0.0 0.0 0.0 0.0 5.207457287133467E-5 6 0.0 0.0 0.0 0.0 5.207457287133467E-5 7 0.0 0.0 0.0 5.207457287133467E-5 1.0414914574266934E-4 8 0.0 0.0 0.0 2.0829829148533868E-4 1.0414914574266934E-4 9 0.0 0.0 0.0 3.12447437228008E-4 1.0414914574266934E-4 10-11 0.0 0.0 0.0 3.645220100993427E-4 1.0414914574266934E-4 12-13 0.0 0.0 0.0 4.1659658297067735E-4 1.56223718614004E-4 14-15 0.0 0.0 0.0 4.6867115584201197E-4 2.864101507923407E-4 16-17 0.0 0.0 0.0 4.6867115584201197E-4 3.645220100993427E-4 18-19 0.0 0.0 0.0 5.46783015149014E-4 3.645220100993427E-4 20-21 0.0 0.0 0.0 5.728203015846813E-4 3.645220100993427E-4 22-23 0.0 0.0 0.0 6.509321608916833E-4 3.9055929653501005E-4 24-25 0.0 0.0 0.0 9.373423116840239E-4 5.207457287133466E-4 26-27 0.0 0.0 0.0 0.0011716778896050301 6.24894874456016E-4 28-29 0.0 0.0 0.0 0.0018486473369323806 6.24894874456016E-4 30-31 0.0 0.0 0.0 0.005519904724361474 6.24894874456016E-4 32-33 0.0 0.0 0.0 0.013357127941497342 6.24894874456016E-4 34-35 0.0 0.0 0.0 0.024449011963091627 6.769694473273506E-4 36-37 0.0 0.0 0.0 0.04499243096083315 6.769694473273506E-4 38-39 0.0 0.0 0.0 0.07701829327670398 6.769694473273506E-4 40-41 0.0 0.0 0.0 0.1142255755932726 6.769694473273506E-4 42-43 2.6037286435667335E-5 0.0 0.0 0.15244831208083223 6.769694473273506E-4 44-45 5.207457287133467E-5 0.0 0.0 0.20064332927325246 7.03006733763018E-4 46-47 5.207457287133467E-5 0.0 0.0 0.2633150777239037 8.59230452377022E-4 48-49 5.207457287133467E-5 0.0 0.0 0.3312463580345598 8.852677388126893E-4 50-51 5.207457287133467E-5 0.0 0.0 0.404723580356013 8.852677388126893E-4 52-53 5.207457287133467E-5 0.0 0.0 0.48153357534123165 9.113050252483566E-4 54-55 5.207457287133467E-5 0.0 0.0 0.5713101389714126 9.373423116840239E-4 56-57 1.56223718614004E-4 0.0 0.0 0.6804584437097301 9.373423116840239E-4 58-59 1.56223718614004E-4 0.0 0.0 0.8046302627214277 9.894168845553586E-4 60-61 1.56223718614004E-4 0.0 0.0 0.9407531962070964 0.0010414914574266934 62-63 1.56223718614004E-4 0.0 0.0 1.080104753210788 0.001093566030298028 64-65 1.56223718614004E-4 0.0 0.0 1.2290640689092407 0.0011456406031693626 66-67 1.56223718614004E-4 0.0 0.0 1.407497592852869 0.0011456406031693626 68-69 1.56223718614004E-4 0.0 0.0 1.6143377962978103 0.0011456406031693626 70-71 1.56223718614004E-4 0.0 0.0 1.8432836559266332 0.0011456406031693626 72-73 1.56223718614004E-4 0.0 0.0 2.0712400986709003 0.0011456406031693626 74-75 1.56223718614004E-4 0.0 0.0 2.307424323928839 0.0011456406031693626 76-77 1.56223718614004E-4 0.0 0.0 2.570765438939178 0.0011456406031693626 78-79 1.56223718614004E-4 0.0 0.0 2.8705066803865806 0.0011456406031693626 80-81 1.56223718614004E-4 0.0 0.0 3.2012322926924273 0.00117167788960503 82-83 1.56223718614004E-4 0.0 0.0 3.5446641007788795 0.0012758270353476994 84-85 1.8226100504967134E-4 0.0 0.0 3.8862993361012705 0.0013018643217833667 86-87 2.0829829148533868E-4 0.0 0.0 4.2453014414762515 0.0013018643217833667 88-89 2.0829829148533868E-4 0.0 0.0 4.647603554193747 0.0013018643217833667 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6765 0.0 36.319347 1 GTATCAA 10670 0.0 34.31855 1 TATCAAC 12940 0.0 28.029203 2 ATCAACG 12935 0.0 27.96673 3 TCAACGC 12925 0.0 27.915003 4 CAACGCA 13175 0.0 27.45728 5 GTATACG 70 0.008451359 27.104288 1 AACGCAG 13530 0.0 26.753769 6 GTACATG 8880 0.0 24.303818 1 ACGCAGA 15175 0.0 23.761179 7 CGCAGAG 15250 0.0 23.520039 8 TACATGG 9035 0.0 23.299063 2 ACATGGG 8820 0.0 22.791918 3 GAGTACT 8705 0.0 22.50706 12-13 GCAGAGT 16140 0.0 21.900162 9 GTACTTT 9275 0.0 21.047796 14-15 GTACCTG 1740 0.0 20.717646 1 TACCTGG 1580 0.0 20.705017 2 AGTACTT 9170 0.0 20.435682 12-13 CATGGGG 3575 0.0 20.15817 4 >>END_MODULE