FastQCFastQC Report
Thu 26 May 2016
SRR938683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920323
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120420.6270820065166121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114730.5974515745528226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79950.41633621010632066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31610.16460772484628888No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30590.15929611841341274No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30450.15856707439321407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24120.1256038697656592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97300.032.1428341
GTATCAA136500.027.8292731
TATCAAC169600.022.1338632
ATCAACG173600.021.5965293
GAGTACT109350.021.59140212-13
TCAACGC174500.021.5123394
CAACGCA177200.021.238125
AACGCAG181250.020.8421086
GTACATG88800.020.4241331
AGTACTT113650.020.2733912-13
GTACTTT116050.020.18127614-15
AGAGTAC166450.019.31666410-11
TACATGG91250.019.1911092
ACGCAGA200450.018.7510577
CGCAGAG201200.018.5396338
ACATGGG90000.018.4557693
GTGTAGC10700.017.7954031
TACTTTT129250.017.71631414-15
ACTTTTT133250.017.62968416-17
GCAGAGT208550.017.6131639
Produced by FastQC (version 0.11.5)