##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938683_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1920323 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.452860794772548 31.0 31.0 34.0 30.0 34.0 2 31.70996233446144 31.0 31.0 34.0 30.0 34.0 3 31.748239749250516 31.0 31.0 34.0 30.0 34.0 4 35.52176899407027 37.0 35.0 37.0 33.0 37.0 5 35.39056033802647 37.0 35.0 37.0 33.0 37.0 6 35.462298790359746 37.0 35.0 37.0 33.0 37.0 7 35.401581921374685 37.0 35.0 37.0 33.0 37.0 8 35.353021861426434 37.0 35.0 37.0 32.0 37.0 9 36.92908692964673 39.0 37.0 39.0 33.0 39.0 10-11 36.811523113559545 39.0 37.0 39.0 32.5 39.0 12-13 36.71262464699949 39.0 35.0 39.0 32.0 39.0 14-15 37.71910506722047 39.0 37.0 41.0 32.0 41.0 16-17 37.58107203840187 39.0 36.0 41.0 32.0 41.0 18-19 37.611513792211 39.0 36.5 40.5 32.0 41.0 20-21 37.73722103000381 39.0 37.0 41.0 32.0 41.0 22-23 37.670620515402874 39.0 37.0 41.0 32.0 41.0 24-25 37.566748666760745 39.0 36.0 41.0 32.0 41.0 26-27 37.332824477965424 39.0 36.0 41.0 31.5 41.0 28-29 37.10838697448294 39.0 36.0 40.5 31.0 41.0 30-31 36.787195435351244 39.0 35.5 40.0 30.5 41.0 32-33 36.46844879741585 38.0 35.0 40.0 30.0 41.0 34-35 36.365656715042206 38.0 35.0 40.0 30.0 41.0 36-37 36.227137570085866 38.0 35.0 40.0 30.0 41.0 38-39 36.09006531713675 38.0 35.0 40.0 30.0 41.0 40-41 35.859125522112684 38.0 35.0 40.0 29.0 41.0 42-43 35.59515248216056 38.0 35.0 40.0 28.0 41.0 44-45 35.22729275231302 38.0 34.0 40.0 26.5 41.0 46-47 34.80487917917975 38.0 34.0 40.0 25.5 41.0 48-49 34.41978719205051 38.0 33.0 40.0 24.0 41.0 50-51 34.097659872844304 38.0 33.0 40.0 23.5 41.0 52-53 33.812053493084235 37.5 33.0 40.0 23.0 41.0 54-55 33.50589432090331 37.0 32.5 40.0 22.0 41.0 56-57 33.169617819502236 37.0 32.0 39.5 20.5 41.0 58-59 32.791208041563834 36.0 31.0 39.0 19.5 40.0 60-61 32.34546974649577 36.0 31.0 39.0 18.0 40.0 62-63 31.88004153467932 35.0 30.5 39.0 15.5 40.0 64-65 31.439944998836133 35.0 30.0 38.0 13.0 40.0 66-67 30.962126944269272 35.0 29.0 38.0 9.5 40.0 68-69 30.412395987550013 34.0 29.0 37.5 8.0 39.5 70-71 29.83461063581491 34.0 28.0 37.0 7.0 39.0 72-73 29.35495591106288 34.0 28.0 36.0 3.5 39.0 74-75 28.840382841844836 33.0 26.5 36.0 2.0 38.5 76-77 27.06672236910145 30.5 25.5 34.0 2.0 36.0 78-79 28.129837792912962 32.5 26.0 35.0 2.0 37.0 80-81 28.15598209259588 33.0 26.0 35.0 2.0 37.0 82-83 27.728556341823744 33.0 26.0 35.0 2.0 36.0 84-85 27.180563894719796 32.5 25.5 35.0 2.0 36.0 86-87 26.433140414399034 32.0 24.5 34.5 2.0 35.5 88-89 25.724247952037235 31.5 22.5 34.0 2.0 35.0 90-91 25.073440249374713 31.0 20.0 34.0 2.0 35.0 92-93 24.52959710423715 31.0 18.5 34.0 2.0 35.0 94-95 23.82550669861268 31.0 10.0 34.0 2.0 35.0 96-97 22.882588762411324 30.0 2.0 34.0 2.0 35.0 98-99 21.65372335799759 29.5 2.0 34.0 2.0 35.0 100-101 18.322987330777167 24.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12310671849596844 1101 2 0.0977820317195146 1101 3 0.1686446456975368 1101 4 0.14485080596286082 1101 5 0.11890140409052208 1101 6 0.1910345394914259 1101 7 0.055192591885571574 1101 8 0.11223126443601927 1101 9 0.11381745845531555 1101 10-11 0.16717113414004814 1101 12-13 0.21053307023717593 1101 14-15 0.2879791832389529 1101 16-17 0.2650344895958483 1101 18-19 0.2575123830480024 1101 20-21 0.44473907937681645 1101 22-23 0.24581668934117573 1101 24-25 0.19968860356897267 1101 26-27 0.15338830756192579 1101 28-29 0.19575209055525988 1101 30-31 0.17643077124065343 1101 32-33 0.23950822012088224 1101 34-35 0.30108123107815743 1101 36-37 0.2127375611654756 1101 38-39 0.26386661956647117 1101 40-41 0.4447728010875025 1101 42-43 0.4386908163627936 1101 44-45 0.42855285833405077 1101 46-47 0.510397872135087 1101 48-49 0.47719436314698527 1101 50-51 0.7041416555717319 1101 52-53 0.7133049770301909 1101 54-55 0.7934459524486996 1101 56-57 0.6129942942121573 1101 58-59 0.6187709550238694 1101 60-61 0.611122733745205 1101 62-63 0.6291982718560725 1101 64-65 0.5366488061926695 1101 66-67 0.45060960194780364 1101 68-69 0.39569056065890607 1101 70-71 0.3589525977230288 1101 72-73 0.5036581763490524 1101 74-75 0.7499058868794926 1101 76-77 0.7902638773796795 1101 78-79 0.8510859455705706 1101 80-81 0.7711645305259189 1101 82-83 0.869986751223756 1101 84-85 0.8755429640476393 1101 86-87 1.0966985503272362 1101 88-89 1.2278491956688171 1101 90-91 1.2545042872245133 1101 92-93 1.429301520313917 1101 94-95 1.25344954820131 1101 96-97 1.293375857332144 1101 98-99 1.41821302082408 1101 100-101 2.089253865073026 1102 1 -0.038805310386926806 1102 2 0.036297277744900214 1102 3 0.0928158131258705 1102 4 0.10562694597702205 1102 5 0.040427703557455175 1102 6 0.06328577854184658 1102 7 0.04272168835888834 1102 8 0.04470076360803432 1102 9 -0.02511628526166021 1102 10-11 0.09910440388732411 1102 12-13 0.12702709880409913 1102 14-15 0.2483755623444992 1102 16-17 0.3722238492686287 1102 18-19 0.3444414689886557 1102 20-21 0.3083131620113875 1102 22-23 0.18422719391410425 1102 24-25 0.05897039318494279 1102 26-27 0.20023886667063096 1102 28-29 0.26464376214691754 1102 30-31 0.2666011077591435 1102 32-33 0.3043595633276084 1102 34-35 0.2504446488093066 1102 36-37 0.1561296628407618 1102 38-39 0.3747415910405181 1102 40-41 0.46592238241425576 1102 42-43 0.3110698516881669 1102 44-45 0.35981835743584156 1102 46-47 0.25073281072383224 1102 48-49 0.36711612888892375 1102 50-51 0.3909850758775306 1102 52-53 0.31489552572326573 1102 54-55 0.42240749068518113 1102 56-57 0.12445909464751281 1102 58-59 0.30820646296139387 1102 60-61 0.1976248958662481 1102 62-63 0.09073264636235123 1102 64-65 0.18307794187088788 1102 66-67 0.1567034479486118 1102 68-69 0.3645060460097298 1102 70-71 0.4780388309512311 1102 72-73 0.4246817168442014 1102 74-75 0.31685393160818975 1102 76-77 0.3016189040916757 1102 78-79 0.2890944536192812 1102 80-81 0.4189994252285736 1102 82-83 0.38449455416939315 1102 84-85 0.47301781732183557 1102 86-87 0.5159612641256075 1102 88-89 0.5700963971995634 1102 90-91 0.5305692854398103 1102 92-93 0.7437405098807339 1102 94-95 0.83555771861975 1102 96-97 0.9602921870512162 1102 98-99 1.4127582732275776 1102 100-101 1.556712977871964 1103 1 0.0836365664956027 1103 2 0.08491550063646613 1103 3 0.052366359059604406 1103 4 0.03603380779931342 1103 5 0.030222272458054533 1103 6 -0.00821883365694731 1103 7 0.10291135326852441 1103 8 0.11950615705683276 1103 9 0.188132726410025 1103 10-11 0.017501429489733766 1103 12-13 0.12181919688391218 1103 14-15 0.21536116098907598 1103 16-17 8.713690351953574E-4 1103 18-19 0.12512235660160798 1103 20-21 0.1218759679225343 1103 22-23 0.07849736051802125 1103 24-25 0.0765163975147587 1103 26-27 0.14533581697183706 1103 28-29 0.18556067197372528 1103 30-31 0.1626326166070342 1103 32-33 0.3689109328682747 1103 34-35 0.25807524933641446 1103 36-37 0.20001402842810734 1103 38-39 0.38588440448178574 1103 40-41 0.41783373877721175 1103 42-43 0.4957571163718981 1103 44-45 0.5382289137234935 1103 46-47 0.6424582672373873 1103 48-49 0.6923901546795861 1103 50-51 0.7171360077299411 1103 52-53 0.6991202762880206 1103 54-55 0.7512997393674112 1103 56-57 0.6342594287966037 1103 58-59 0.4711005000472426 1103 60-61 0.5195574306930553 1103 62-63 0.49542379376446277 1103 64-65 0.6480152300862478 1103 66-67 0.5233759583025268 1103 68-69 0.5799644420940666 1103 70-71 0.6049504226304485 1103 72-73 0.5458224124390831 1103 74-75 0.45542379700728475 1103 76-77 0.4049587478416754 1103 78-79 0.3555096456373512 1103 80-81 0.4769497029017664 1103 82-83 0.39331314886065627 1103 84-85 0.2768227636697276 1103 86-87 0.6815016679283161 1103 88-89 0.790767519187515 1103 90-91 1.1318790557711367 1103 92-93 1.2005396203852534 1103 94-95 1.095456414967643 1103 96-97 1.2113334615241058 1103 98-99 1.6426852075272755 1103 100-101 1.7016270874352166 1104 1 -0.0473345895985382 1104 2 0.04614134228235045 1104 3 0.020721350487285406 1104 4 0.0905189328772451 1104 5 0.04580221907588822 1104 6 0.11922184199766406 1104 7 -0.032981823944695066 1104 8 0.07584699441655829 1104 9 0.0434784928532892 1104 10-11 0.02723588241990882 1104 12-13 0.06423768679195518 1104 14-15 0.05825928676837577 1104 16-17 0.16087090632478152 1104 18-19 0.22832272969232292 1104 20-21 0.1982155145637421 1104 22-23 0.07237850238990262 1104 24-25 0.14580691633489806 1104 26-27 0.3035564762611145 1104 28-29 0.3475503510962312 1104 30-31 0.12145613885609663 1104 32-33 0.2018027024677167 1104 34-35 0.17539011646546498 1104 36-37 -0.10157306828323698 1104 38-39 0.18139964470154268 1104 40-41 0.33299566306804707 1104 42-43 0.3712945910445882 1104 44-45 0.28166073943265957 1104 46-47 0.2550509310855773 1104 48-49 0.46361163710633235 1104 50-51 0.6708871279762008 1104 52-53 0.5204364925832223 1104 54-55 0.33518097865201213 1104 56-57 0.5975444535394345 1104 58-59 0.40828938817847416 1104 60-61 0.4624766219726695 1104 62-63 0.20261078234769414 1104 64-65 0.3443674890871833 1104 66-67 0.3731839334817657 1104 68-69 0.5302050515080339 1104 70-71 0.29630881863262815 1104 72-73 0.2904197813215639 1104 74-75 0.27682208351757254 1104 76-77 0.48012264474005306 1104 78-79 0.46648952016976963 1104 80-81 0.40427238740145555 1104 82-83 0.5407286676807921 1104 84-85 0.47435663894248137 1104 86-87 0.7373036911904727 1104 88-89 1.1981173087760446 1104 90-91 1.156777259480613 1104 92-93 1.3480394012066377 1104 94-95 1.4415349442304226 1104 96-97 1.2890125548065257 1104 98-99 1.5312181476379365 1104 100-101 2.077446084863521 1105 1 0.04114610222359616 1105 2 0.09295190032940326 1105 3 0.050496196892254375 1105 4 -0.0019346823171133565 1105 5 0.023918596504884704 1105 6 0.006869406811325973 1105 7 0.06549511574293376 1105 8 -0.008667920956220598 1105 9 0.02317612696657534 1105 10-11 0.141327955206215 1105 12-13 0.0404608954029797 1105 14-15 0.11391760770095516 1105 16-17 0.13548031422774898 1105 18-19 0.012576527133205673 1105 20-21 0.11155384223484077 1105 22-23 -0.011945870914296108 1105 24-25 -0.12493460243725707 1105 26-27 -0.02531773023900996 1105 28-29 0.10176946043649338 1105 30-31 -0.06038672072983786 1105 32-33 0.12448818425964703 1105 34-35 0.24022674546614553 1105 36-37 0.25362950869294565 1105 38-39 0.16426693910626966 1105 40-41 0.21370943646464724 1105 42-43 0.248965179159768 1105 44-45 0.3298620120954254 1105 46-47 0.5443034905773345 1105 48-49 0.4736214130758256 1105 50-51 0.6618882587214969 1105 52-53 0.627454132720338 1105 54-55 0.5864988201792016 1105 56-57 0.5745349353696625 1105 58-59 0.49433833949517236 1105 60-61 0.29853769753351145 1105 62-63 0.3331452445545118 1105 64-65 0.39652764652368333 1105 66-67 0.44724481505470237 1105 68-69 0.4261993288647936 1105 70-71 0.4456685309179349 1105 72-73 0.18482488932943753 1105 74-75 0.49341902514508007 1105 76-77 0.7052627116658812 1105 78-79 0.55990107827553 1105 80-81 0.4862033072870027 1105 82-83 0.5309902195329954 1105 84-85 0.6003595691808599 1105 86-87 0.8402577951833052 1105 88-89 0.951281608875334 1105 90-91 1.1804682877951933 1105 92-93 1.2872405283784722 1105 94-95 1.4318554581962744 1105 96-97 1.2112879818989768 1105 98-99 1.3821796640107706 1105 100-101 1.559582223534246 1106 1 -0.1209104410925157 1106 2 -0.20497108552541476 1106 3 -0.10493057713491183 1106 4 -0.0715953636269262 1106 5 -0.012954283922496757 1106 6 -0.1454103113005516 1106 7 -0.05139011132024507 1106 8 -0.08665536587071188 1106 9 -0.07960934890002846 1106 10-11 -0.06956714916561424 1106 12-13 -0.05240478014774652 1106 14-15 0.0029126663741934067 1106 16-17 -0.14867897875363667 1106 18-19 -0.23068640543331753 1106 20-21 -0.1969037369159139 1106 22-23 -0.12928313984914297 1106 24-25 -0.10610117245096973 1106 26-27 -0.11629012134409322 1106 28-29 -0.09252569898743701 1106 30-31 -0.08932956008193571 1106 32-33 -0.06210997015881503 1106 34-35 0.07556591717257533 1106 36-37 0.0660809038360739 1106 38-39 -0.07926136595717992 1106 40-41 -0.02857886015392097 1106 42-43 0.14303625516106422 1106 44-45 0.08422742975646003 1106 46-47 0.26535610809084886 1106 48-49 0.15171709148604862 1106 50-51 0.0922351938428605 1106 52-53 0.168812289383645 1106 54-55 0.1467844764349735 1106 56-57 -0.09541570481142259 1106 58-59 -0.018935630215480614 1106 60-61 0.0665128759851541 1106 62-63 0.27454843169690335 1106 64-65 0.3201662428555032 1106 66-67 0.0457518609216514 1106 68-69 -0.03283643094771094 1106 70-71 0.032876667938868565 1106 72-73 0.2320578532435853 1106 74-75 0.4226188188333282 1106 76-77 0.3844909667548606 1106 78-79 0.21619879788930874 1106 80-81 0.28483729367363253 1106 82-83 0.6055307616625498 1106 84-85 0.5343717447810192 1106 86-87 0.636003417071592 1106 88-89 0.7291721781300744 1106 90-91 0.8555414456210073 1106 92-93 1.1247855254521575 1106 94-95 1.200775875843945 1106 96-97 1.088069091011704 1106 98-99 1.2186659771503194 1106 100-101 1.5157296188484004 1107 1 -0.07713047988323751 1107 2 -0.06280622632865729 1107 3 -0.1047941164414361 1107 4 -0.07450915281690129 1107 5 -0.03853794560870938 1107 6 -0.10624364576652567 1107 7 0.04390430534426315 1107 8 0.11351181207049876 1107 9 0.039626851784177575 1107 10-11 0.05000911591607604 1107 12-13 -0.06035660571726709 1107 14-15 -0.11482219484773282 1107 16-17 -0.02760844189498357 1107 18-19 -0.08606335091563722 1107 20-21 -0.010344163804106188 1107 22-23 -0.10071011910685002 1107 24-25 -0.12310357682314788 1107 26-27 -0.08407452432176399 1107 28-29 -0.10344527280601312 1107 30-31 -0.17730022375118182 1107 32-33 -0.06798729417005234 1107 34-35 -0.08993304102342847 1107 36-37 0.042893459307201454 1107 38-39 -0.13823671468628618 1107 40-41 0.035865246008143004 1107 42-43 0.00917048953366617 1107 44-45 -0.1396367456417451 1107 46-47 -0.2170198030629109 1107 48-49 -0.4235441070574666 1107 50-51 -0.39540328861635743 1107 52-53 -0.3085083239283435 1107 54-55 -0.30250460956381175 1107 56-57 -0.33416687199888173 1107 58-59 -0.16489828293525477 1107 60-61 -0.20244748109605126 1107 62-63 -0.2473753922470543 1107 64-65 -0.1801973351873727 1107 66-67 -0.3143556766288249 1107 68-69 -0.3555885332443012 1107 70-71 -0.5091611088391623 1107 72-73 -0.05747830272391852 1107 74-75 -0.031183799744084695 1107 76-77 0.07115560372728424 1107 78-79 -0.04938048726868871 1107 80-81 0.040831302451156404 1107 82-83 -0.012148509495993665 1107 84-85 0.025857766394544512 1107 86-87 0.3051551453245267 1107 88-89 0.5702006350776756 1107 90-91 0.5995975902258479 1107 92-93 0.7186818995936726 1107 94-95 1.0162789795092237 1107 96-97 1.1486761236304304 1107 98-99 1.139581024931946 1107 100-101 1.44450773838199 1108 1 -0.21093465678807632 1108 2 -0.1455168180163824 1108 3 -0.10148220797407603 1108 4 -0.007602344240844161 1108 5 -0.07946492394738414 1108 6 -0.03421968372171591 1108 7 -0.18499040353199092 1108 8 -0.24452952542373652 1108 9 -0.1476757459021485 1108 10-11 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 18.0 4 44.0 5 89.0 6 278.0 7 615.0 8 1334.0 9 2433.0 10 3856.0 11 5545.0 12 7492.0 13 10184.0 14 12858.0 15 16056.0 16 20477.0 17 26138.0 18 24196.0 19 18833.0 20 17724.0 21 18906.0 22 20791.0 23 23243.0 24 26609.0 25 31246.0 26 37571.0 27 44410.0 28 53297.0 29 62848.0 30 74602.0 31 88509.0 32 106395.0 33 130807.0 34 164882.0 35 215314.0 36 264481.0 37 269822.0 38 113867.0 39 4544.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.14255806264074 22.24262852707396 16.09007537970614 20.52473803057916 2 17.762688880985124 23.144648061810436 37.63231498034445 21.460348076859987 3 21.143838822948013 26.067073091349734 30.54522598542016 22.24386210028209 4 15.000289013879437 19.362680132456884 35.61354001384142 30.02349083982226 5 13.337964498680691 38.37182598969028 34.134830442587 14.155379069042032 6 30.858194168376883 37.77703021835389 16.741558581551125 14.623217031718102 7 27.194123072004032 34.00318592236827 20.81983083054257 17.98286017508513 8 25.855129579763403 36.304048850115315 19.168702348511165 18.672119221610114 9 26.002188173552053 17.05811991003597 20.353919627062737 36.58577228934924 10-11 25.01508860748947 27.56684682733061 26.77786497375702 20.6401995914229 12-13 26.036609466220007 24.964081563361997 27.736922382328387 21.26238658808961 14-15 22.629141719685105 26.207108441420644 26.789302317709655 24.374447521184592 16-17 21.368624969731055 29.469323563950145 27.44225466532312 21.719796800995685 18-19 21.174075154140976 27.453054699216793 30.570269538410265 20.802600608231963 20-21 22.16194879715548 26.28016224353924 30.91727277129941 20.640616188005872 22-23 21.801644827458713 26.476561495123473 30.810181412189515 20.911612265228296 24-25 21.6140982532626 26.70548131746586 30.90792538546901 20.77249504380253 26-27 21.494427233780186 26.775080832839926 30.901864844471316 20.828627088908572 28-29 21.153446581642775 26.957392063730946 31.038710675235365 20.850450679390914 30-31 21.416995994944603 26.753025402497393 30.920735730395354 20.90924287216265 32-33 21.3463307995582 26.917138418901406 30.79515789791613 20.941372883624265 34-35 21.741723037279336 26.86772951896672 30.66667222170433 20.72387522204961 36-37 21.760862749101303 26.697394065214514 30.60686915951129 20.93487402617289 38-39 21.38058882395356 27.048450991620037 30.7417965221363 20.829163662290107 40-41 21.68376028982994 26.815518806891404 30.636058808625634 20.864662094653024 42-43 21.534273398229146 27.014371770452662 30.244876012722248 21.206478818595944 44-45 21.779753870252797 27.051150660734223 29.95891762069023 21.21017784832275 46-47 21.864352233013957 27.366105347245767 29.46067717649926 21.30886524324102 48-49 21.989359076238255 27.488801357272376 29.252411835098492 21.26942773139088 50-51 21.8153271728857 27.682203180207416 29.090930394232196 21.41153925267469 52-53 22.00028328567642 27.84659143279542 28.767946850607945 21.385178430920217 54-55 21.778497679817406 27.64279759186345 28.950728601386327 21.627976126932815 56-57 21.938236432100226 27.83643689108551 28.759302471511305 21.46602420530296 58-59 22.167462673431878 27.50322928874411 28.88604965488726 21.443258382936754 60-61 21.96456087490177 27.653781400578136 29.1353934553173 21.24626426920279 62-63 21.868899138322043 27.595253506831924 29.132390748847982 21.403456605998052 64-65 21.70679099297358 27.64021990050632 29.03646938561898 21.61651972090112 66-67 21.79695079370287 27.784156979393355 28.67115241709251 21.747739809811264 68-69 21.74820165801312 27.626990530958405 28.790526323737748 21.834281487290728 70-71 21.798608543501242 27.60765301074317 28.783386884272687 21.810351561482904 72-73 21.877829927569476 27.50170153666857 28.787735188299052 21.832733347462902 74-75 21.64326001407055 27.874190956417227 28.47609490695055 22.00645412256167 76-77 21.736142940488104 27.708855505210494 28.60899535325115 21.946006201050256 78-79 21.71062947109717 27.617960390500347 28.636978555402926 22.034431582999556 80-81 21.588873743863335 27.75624927583778 28.564186484301462 22.090690495997418 82-83 21.683383472467913 27.71817553609471 28.536683672486348 22.06175731895103 84-85 21.57806264883564 27.609235529647876 28.48119821509194 22.331503606424544 86-87 21.614875192362188 27.508125290188936 28.510741911272643 22.366257606176237 88-89 21.62350821298932 27.45018671819915 28.4055571514376 22.52074791737393 90-91 21.60293607102118 27.383169275666475 28.55503958527475 22.458855068037597 92-93 21.557024521395622 27.398385584091844 28.48427061489135 22.56031927962119 94-95 21.42744810505738 27.304925944711357 28.472099991563915 22.795525958667348 96-97 21.45034975886869 27.173110981850453 28.49491986504354 22.881619394237322 98-99 21.385020245339508 27.13471749608203 28.32259336146582 23.15766889711264 100-101 21.566181457917548 26.52192422307365 28.8546235433648 23.057270775644003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3266.0 1 2092.5 2 1109.5 3 1326.0 4 1135.0 5 1041.5 6 1946.5 7 4318.5 8 6244.0 9 6486.5 10 5123.0 11 3442.0 12 3086.0 13 3196.0 14 3348.5 15 3510.5 16 3676.0 17 3910.0 18 4227.0 19 4529.5 20 4937.5 21 5622.0 22 6514.5 23 7662.5 24 9091.5 25 10950.0 26 13444.0 27 16362.0 28 19176.0 29 22240.0 30 25671.0 31 29337.0 32 33165.0 33 36785.0 34 40931.5 35 44115.5 36 47293.0 37 51311.0 38 53583.5 39 54473.5 40 55482.0 41 57922.5 42 60938.5 43 62970.0 44 64450.5 45 65946.5 46 66796.5 47 68387.5 48 73428.0 49 74270.0 50 69690.0 51 65969.5 52 62665.5 53 60711.5 54 58528.5 55 53990.5 56 48822.0 57 44334.0 58 39635.0 59 34419.5 60 29224.5 61 23996.5 62 19394.0 63 15302.0 64 11646.5 65 8827.5 66 6641.5 67 5206.5 68 3669.5 69 2343.5 70 1673.0 71 1248.5 72 996.5 73 805.0 74 602.5 75 476.5 76 356.0 77 209.5 78 115.0 79 74.5 80 47.5 81 26.5 82 14.5 83 6.0 84 3.5 85 3.0 86 2.0 87 2.0 88 1.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3063026376291905 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.6037286435667335E-5 16-17 0.0019788337691107175 18-19 1.56223718614004E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.0414914574266934E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007498738493472192 36-37 0.016715937891698427 38-39 0.010805473870801942 40-41 0.008462118091591883 42-43 0.0034108845230724203 44-45 0.0018226100504967133 46-47 0.0013539388946547013 48-49 5.207457287133467E-5 50-51 4.1659658297067735E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.4263386940634466E-4 60-61 8.331931659413547E-4 62-63 0.0 64-65 0.0 66-67 0.0010414914574266934 68-69 6.509321608916833E-4 70-71 0.0017184609047540438 72-73 0.0 74-75 0.0 76-77 0.07574246624135628 78-79 0.032911130054683504 80-81 2.6037286435667335E-5 82-83 0.0 84-85 0.0 86-87 0.12453634102179685 88-89 0.2954190518990816 90-91 0.2519367835515171 92-93 0.0 94-95 5.207457287133467E-5 96-97 0.0 98-99 2.34335577921006E-4 100-101 8.071558795056874E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1920323.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.9286615367071 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4504460750692 47.01430230236514 2 12.808136659683203 15.351489735880897 3 4.009306106614024 7.208170459809743 4 1.707444941935115 4.092995600711681 5 0.8640073375234153 2.58894016478361 6 0.47869975497408523 1.721270135612794 7 0.31451266832446884 1.3193826274316598 8 0.2242182730752368 1.0749680797976664 9 0.16432113660189582 0.8862791200867824 >10 0.8793091413349089 9.68680985037873 >50 0.06589422121295642 2.707692641305406 >100 0.03127229431904204 3.2667673477955192 >500 0.001476200666177043 0.5666554258118606 >1k 6.946826664362552E-4 0.874519646502738 >5k 8.68353333045319E-5 0.4160538276578036 >10k+ 1.736706666090638E-4 1.223703034067949 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12042 0.6270820065166121 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11473 0.5974515745528226 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7995 0.41633621010632066 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3161 0.16460772484628888 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3059 0.15929611841341274 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3045 0.15856707439321407 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2412 0.1256038697656592 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 5.207457287133467E-5 5 1.0414914574266934E-4 0.0 0.0 0.0 5.207457287133467E-5 6 1.0414914574266934E-4 0.0 0.0 0.0 5.207457287133467E-5 7 1.0414914574266934E-4 0.0 0.0 0.0 5.207457287133467E-5 8 1.0414914574266934E-4 0.0 0.0 0.0 5.207457287133467E-5 9 1.0414914574266934E-4 0.0 0.0 5.207457287133467E-5 5.207457287133467E-5 10-11 1.0414914574266934E-4 0.0 0.0 5.207457287133467E-5 5.207457287133467E-5 12-13 1.0414914574266934E-4 0.0 0.0 1.0414914574266934E-4 5.207457287133467E-5 14-15 1.0414914574266934E-4 0.0 0.0 1.8226100504967134E-4 5.207457287133467E-5 16-17 1.0414914574266934E-4 0.0 0.0 2.0829829148533868E-4 1.0414914574266934E-4 18-19 1.3018643217833667E-4 0.0 0.0 2.6037286435667335E-4 1.56223718614004E-4 20-21 1.56223718614004E-4 0.0 0.0 3.12447437228008E-4 1.56223718614004E-4 22-23 1.56223718614004E-4 0.0 0.0 4.1659658297067735E-4 1.56223718614004E-4 24-25 1.56223718614004E-4 0.0 0.0 8.331931659413547E-4 2.0829829148533868E-4 26-27 1.56223718614004E-4 0.0 0.0 0.0010675287438623605 2.0829829148533868E-4 28-29 1.56223718614004E-4 0.0 0.0 0.0017444981911897113 2.0829829148533868E-4 30-31 1.56223718614004E-4 0.0 0.0 0.005233494573569134 2.0829829148533868E-4 32-33 1.56223718614004E-4 0.0 0.0 0.01288845678565533 2.0829829148533868E-4 34-35 1.56223718614004E-4 0.0 0.0 0.02379807980219994 2.6037286435667335E-4 36-37 2.0829829148533868E-4 0.0 0.0 0.043820753071228125 2.6037286435667335E-4 38-39 2.0829829148533868E-4 0.0 0.0 0.0750915340804646 3.12447437228008E-4 40-41 2.0829829148533868E-4 0.0 0.0 0.11180410795475554 3.12447437228008E-4 42-43 2.0829829148533868E-4 0.0 0.0 0.14940194956785915 3.12447437228008E-4 44-45 2.6037286435667335E-4 0.0 0.0 0.19665962444859536 3.12447437228008E-4 46-47 3.12447437228008E-4 0.0 0.0 0.2575087628487499 4.1659658297067735E-4 48-49 3.12447437228008E-4 0.0 0.0 0.3230185755208889 4.6867115584201197E-4 50-51 3.12447437228008E-4 0.0 0.0 0.39456903864610277 4.6867115584201197E-4 52-53 3.12447437228008E-4 0.0 0.0 0.4698688710180527 4.6867115584201197E-4 54-55 3.12447437228008E-4 0.0 0.0 0.5580571601756579 4.6867115584201197E-4 56-57 3.12447437228008E-4 0.0 0.0 0.664497587124666 4.6867115584201197E-4 58-59 3.12447437228008E-4 0.0 0.0 0.7859615283470541 4.6867115584201197E-4 60-61 3.12447437228008E-4 0.0 0.0 0.9186735773096506 4.6867115584201197E-4 62-63 3.12447437228008E-4 0.0 0.0 1.0528176770262085 4.6867115584201197E-4 64-65 3.12447437228008E-4 0.0 0.0 1.196881982874756 4.6867115584201197E-4 66-67 3.645220100993427E-4 0.0 0.0 1.3697174902347156 4.947084422776793E-4 68-69 3.645220100993427E-4 0.0 0.0 1.5689287687540066 5.207457287133467E-4 70-71 3.9055929653501005E-4 0.0 0.0 1.7916777542111406 5.728203015846813E-4 72-73 4.1659658297067735E-4 0.0 0.0 2.0142965532360964 5.728203015846813E-4 74-75 4.1659658297067735E-4 0.0 0.0 2.2443099416087815 5.728203015846813E-4 76-77 4.1659658297067735E-4 0.0 0.0 2.499970057120599 5.988575880203487E-4 78-79 4.1659658297067735E-4 0.0 0.0 2.790650322888389 6.24894874456016E-4 80-81 4.1659658297067735E-4 0.0 0.0 3.109060298710165 6.769694473273506E-4 82-83 4.1659658297067735E-4 0.0 0.0 3.436583324784424 7.8111859307002E-4 84-85 4.6867115584201197E-4 0.0 0.0 3.7597581240239273 7.8111859307002E-4 86-87 4.6867115584201197E-4 0.0 0.0 4.099935271305921 7.8111859307002E-4 88-89 4.6867115584201197E-4 0.0 0.0 4.478725714372009 7.8111859307002E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9730 0.0 32.142834 1 GTATCAA 13650 0.0 27.829273 1 TATCAAC 16960 0.0 22.133863 2 ATCAACG 17360 0.0 21.596529 3 GAGTACT 10935 0.0 21.591402 12-13 TCAACGC 17450 0.0 21.512339 4 CAACGCA 17720 0.0 21.23812 5 AACGCAG 18125 0.0 20.842108 6 GTACATG 8880 0.0 20.424133 1 AGTACTT 11365 0.0 20.27339 12-13 GTACTTT 11605 0.0 20.181276 14-15 AGAGTAC 16645 0.0 19.316664 10-11 TACATGG 9125 0.0 19.191109 2 ACGCAGA 20045 0.0 18.751057 7 CGCAGAG 20120 0.0 18.539633 8 ACATGGG 9000 0.0 18.455769 3 GTGTAGC 1070 0.0 17.795403 1 TACTTTT 12925 0.0 17.716314 14-15 ACTTTTT 13325 0.0 17.629684 16-17 GCAGAGT 20855 0.0 17.613163 9 >>END_MODULE