FastQCFastQC Report
Thu 26 May 2016
SRR938682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875896
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122100.6508889618614251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92560.4934175455355734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64610.34442207883592696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29200.1556589491101852No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.13129725741725554No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.10501648279009071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.036.5181731
GTATCAA106250.032.9042781
TATCAAC129950.026.6890962
ATCAACG129900.026.6263223
TCAACGC131000.026.3665244
CAACGCA133000.025.9700345
GTATACG1109.4782765E-525.9096811
GTACATG87100.025.522971
AACGCAG136050.025.4227076
TACATGG90650.024.0759142
ACATGGG89900.023.2740353
ACGCAGA152050.022.6863167
GAGTACT84300.022.32811712-13
CGCAGAG152850.022.3192428
GTGTAGC10700.021.3088971
CATGGGG35450.021.1462634
GCAGAGT160200.020.9392579
GTACTTT91600.020.65206514-15
AGTACTT88950.020.1201812-13
CATGGGA54300.020.0965164
Produced by FastQC (version 0.11.5)