##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938682_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1875896 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.312366996891086 33.0 31.0 34.0 31.0 34.0 2 32.43475011407882 34.0 31.0 34.0 31.0 34.0 3 32.457944363653425 34.0 31.0 34.0 31.0 34.0 4 35.964423400870835 37.0 35.0 37.0 35.0 37.0 5 35.9798741508058 37.0 37.0 37.0 35.0 37.0 6 36.05247998822963 37.0 37.0 37.0 35.0 37.0 7 35.99666132877302 37.0 37.0 37.0 35.0 37.0 8 35.98158266769586 37.0 37.0 37.0 35.0 37.0 9 37.70695656902088 39.0 38.0 39.0 35.0 39.0 10-11 37.61759740412049 39.0 38.0 39.0 35.0 39.0 12-13 37.539490462157815 39.0 38.0 39.0 35.0 39.0 14-15 38.875973934589126 40.0 38.5 41.0 36.0 41.0 16-17 38.77284241770333 40.0 38.0 41.0 36.0 41.0 18-19 38.729528449338346 40.0 38.0 41.0 35.5 41.0 20-21 38.64484225138281 40.0 38.0 41.0 35.0 41.0 22-23 38.67870820130753 40.0 38.0 41.0 35.0 41.0 24-25 38.63829684588058 40.0 38.0 41.0 35.0 41.0 26-27 38.522367178137806 40.0 38.0 41.0 35.0 41.0 28-29 38.36619700665709 40.0 38.0 41.0 34.5 41.0 30-31 38.1421459398602 40.0 38.0 41.0 34.0 41.0 32-33 37.96904972343883 40.0 38.0 41.0 34.0 41.0 34-35 37.71514572236414 40.0 37.5 41.0 33.5 41.0 36-37 37.532078270863636 40.0 37.0 41.0 33.0 41.0 38-39 37.38027614537266 40.0 37.0 41.0 33.0 41.0 40-41 37.285511030462246 40.0 37.0 41.0 33.0 41.0 42-43 37.2843896996422 40.0 37.0 41.0 33.0 41.0 44-45 37.1060431921599 40.0 37.0 41.0 32.5 41.0 46-47 36.79439345251549 40.0 37.0 41.0 31.0 41.0 48-49 36.50664615735627 40.0 36.5 41.0 30.5 41.0 50-51 35.412758489809676 38.5 35.0 40.0 28.5 40.5 52-53 35.50470201972817 38.5 35.0 40.0 29.5 40.5 54-55 35.98610903802769 39.0 36.0 41.0 30.0 41.0 56-57 35.889012237352176 39.0 35.0 41.0 29.0 41.0 58-59 35.68713004345656 39.0 35.0 41.0 29.0 41.0 60-61 35.39821850465058 39.0 35.0 40.5 28.0 41.0 62-63 35.029561073748226 38.0 35.0 40.0 27.5 41.0 64-65 34.662398128680906 38.0 34.5 40.0 26.5 41.0 66-67 34.286249877391924 37.0 34.0 40.0 26.0 41.0 68-69 33.881872182679636 36.5 34.0 39.0 26.0 41.0 70-71 33.464632101139934 36.0 34.0 39.0 25.0 40.5 72-73 33.04177950163549 35.5 33.5 38.5 24.0 40.0 74-75 32.58510013348288 35.0 33.0 37.5 22.5 40.0 76-77 32.14295568624273 35.0 33.0 37.0 21.5 39.0 78-79 31.71380103161369 35.0 32.0 36.5 20.0 39.0 80-81 31.265542972531527 35.0 32.0 36.0 18.0 38.5 82-83 30.873251502215474 35.0 31.0 36.0 15.5 37.0 84-85 30.54424445704879 35.0 31.0 35.0 9.0 37.0 86-87 30.15187542379748 34.0 31.0 35.0 4.5 36.5 88-89 29.7479441824067 34.0 30.5 35.0 2.0 36.0 90-91 29.345338440937027 34.0 30.0 35.0 2.0 36.0 92-93 28.93230781450571 34.0 29.5 35.0 2.0 35.0 94-95 28.490033562628206 34.0 29.0 35.0 2.0 35.0 96-97 27.9691707322794 34.0 29.0 35.0 2.0 35.0 98-99 27.30022799771416 34.0 28.0 35.0 2.0 35.0 100-101 24.773079637677142 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03065368955079606 1101 2 0.09232842696551558 1101 3 0.09193495461450851 1101 4 0.022799018971532803 1101 5 -0.045351735852904085 1101 6 0.011123878787032027 1101 7 0.0876266099228431 1101 8 0.061163869582649966 1101 9 0.10997291022854938 1101 10-11 0.008296723299814346 1101 12-13 0.024065981852494644 1101 14-15 0.044173572572155706 1101 16-17 0.16905228113384396 1101 18-19 0.31113475902441934 1101 20-21 0.32680909591422136 1101 22-23 0.26794846513808324 1101 24-25 0.22743768877135295 1101 26-27 0.2901358515031447 1101 28-29 0.24672191661579035 1101 30-31 0.1638367065704287 1101 32-33 0.12327335347453783 1101 34-35 0.1383538458219462 1101 36-37 0.1382561949276493 1101 38-39 0.11429365995607554 1101 40-41 0.16287189734926955 1101 42-43 0.2216000841161474 1101 44-45 0.016174645381305197 1101 46-47 -0.009773803600346298 1101 48-49 -0.0228525299468032 1101 50-51 -0.014527999862131935 1101 52-53 0.10994954067780327 1101 54-55 0.03822480618431001 1101 56-57 0.015634897016312266 1101 58-59 0.07690982610582608 1101 60-61 0.032434459111001956 1101 62-63 0.0561759276975593 1101 64-65 0.001117448371047658 1101 66-67 -0.08195493671701826 1101 68-69 0.03235522930901169 1101 70-71 -0.18000786438823724 1101 72-73 -0.02288274002334134 1101 74-75 -0.052514681357450854 1101 76-77 -0.1865078763715431 1101 78-79 -0.2272338495218662 1101 80-81 -0.16477076672732593 1101 82-83 -0.2352896845243002 1101 84-85 -0.1364941754907214 1101 86-87 -0.3538772907234673 1101 88-89 -0.2328107257360763 1101 90-91 -0.2385006023543106 1101 92-93 -0.278744110488109 1101 94-95 -0.3838708771563013 1101 96-97 -0.6378227808435071 1101 98-99 -0.474060379739182 1101 100-101 -0.2408357141350308 1102 1 0.09791430170492532 1102 2 0.027042234259184283 1102 3 0.04655974951035802 1102 4 0.06583958754453079 1102 5 0.04493847483905 1102 6 0.12358916526005714 1102 7 0.11979806017270533 1102 8 0.07478811130467022 1102 9 0.13361081310168998 1102 10-11 0.17486418621287925 1102 12-13 0.16506363833902782 1102 14-15 0.2084519516980734 1102 16-17 0.3350941354622421 1102 18-19 0.37821456182542335 1102 20-21 0.296166115428548 1102 22-23 0.20796157498420342 1102 24-25 0.07791652987216935 1102 26-27 0.16537389379104894 1102 28-29 0.16444968967996232 1102 30-31 0.360693240188823 1102 32-33 0.26557428564721164 1102 34-35 0.30973144489799864 1102 36-37 0.387270153222822 1102 38-39 0.22071514851696605 1102 40-41 0.30326802401112474 1102 42-43 0.320282745480327 1102 44-45 0.23785882610469145 1102 46-47 0.050956881101775764 1102 48-49 0.10576692756907136 1102 50-51 0.3214860929888488 1102 52-53 0.22100269208672074 1102 54-55 0.12504670102894977 1102 56-57 0.10407192864066417 1102 58-59 0.01922369992986006 1102 60-61 -0.03394780269720599 1102 62-63 -0.1283220296706773 1102 64-65 -0.17420314950521032 1102 66-67 -0.07200201255789551 1102 68-69 -0.039086190329406634 1102 70-71 -0.10731619712976936 1102 72-73 -0.16515657415342133 1102 74-75 -0.3092370983533428 1102 76-77 -0.31831882921347443 1102 78-79 -0.3233861143575787 1102 80-81 -0.19448680727347423 1102 82-83 -0.28330064147765555 1102 84-85 -0.24344851435454373 1102 86-87 -0.15255238332401788 1102 88-89 -0.2636945517554281 1102 90-91 -0.42282405767244313 1102 92-93 -0.4483925741225363 1102 94-95 -0.4050736938576627 1102 96-97 -0.6155017026778005 1102 98-99 -0.5475465335738328 1102 100-101 -0.2154988107124538 1103 1 0.07751036984268467 1103 2 0.11679506090647607 1103 3 0.15924386324370943 1103 4 0.2182259950219816 1103 5 0.22453975011437421 1103 6 0.16740249411209618 1103 7 0.15026620550865033 1103 8 0.18471622913745733 1103 9 0.2810054691584156 1103 10-11 0.1646916879466218 1103 12-13 0.3169047165005239 1103 14-15 0.302753568040913 1103 16-17 0.26780099491922726 1103 18-19 0.27000562779748805 1103 20-21 0.29043364868914523 1103 22-23 0.32029657381260535 1103 24-25 0.2269006646707652 1103 26-27 0.23308845744475803 1103 28-29 0.2488564428640032 1103 30-31 0.30157518727957466 1103 32-33 0.24636468179203774 1103 34-35 0.25047978157257944 1103 36-37 0.35284434664905007 1103 38-39 0.3946851412033041 1103 40-41 0.34869722232495093 1103 42-43 0.3501833128216276 1103 44-45 0.44441319217120423 1103 46-47 0.4440712700796894 1103 48-49 0.3704360258111947 1103 50-51 0.5976592965855616 1103 52-53 0.5209329874497612 1103 54-55 0.4616640024535812 1103 56-57 0.5493508300293115 1103 58-59 0.427981750282882 1103 60-61 0.37746825469491796 1103 62-63 0.35273949265965854 1103 64-65 0.28255725352723715 1103 66-67 0.2929997507682387 1103 68-69 0.36331323499195634 1103 70-71 0.23591606250757735 1103 72-73 0.2843447240806469 1103 74-75 0.1699800321824938 1103 76-77 0.2355232099015936 1103 78-79 0.14725142863089147 1103 80-81 0.19475489148415193 1103 82-83 0.3848287180207528 1103 84-85 0.3292249010779891 1103 86-87 0.26163256946854574 1103 88-89 0.2438998216957451 1103 90-91 0.25274381618228503 1103 92-93 0.29535282525308304 1103 94-95 0.27193714882287523 1103 96-97 0.4020398419958866 1103 98-99 0.3544478001137428 1103 100-101 0.7315638903244768 1104 1 -0.1331207992236898 1104 2 -0.08276785979744261 1104 3 -0.06288176686887681 1104 4 0.020027437866623643 1104 5 0.02941040724257249 1104 6 0.08458924891906605 1104 7 0.08646711709668153 1104 8 0.14371046379591235 1104 9 0.08275378634275654 1104 10-11 0.05048057381700488 1104 12-13 0.07436675881994148 1104 14-15 0.13493564416756954 1104 16-17 0.15888735430225154 1104 18-19 0.18184117973083858 1104 20-21 0.22018401845581081 1104 22-23 0.10602690071652177 1104 24-25 0.11897648947682171 1104 26-27 0.11070082123477931 1104 28-29 0.16579205818592868 1104 30-31 0.3440147417484667 1104 32-33 0.30987459757578506 1104 34-35 0.29597587261064007 1104 36-37 0.3086474194855384 1104 38-39 0.2895808519099248 1104 40-41 0.2759784562891596 1104 42-43 0.23199381117654383 1104 44-45 0.31934150271483475 1104 46-47 0.48430610964623355 1104 48-49 0.35955130245702804 1104 50-51 0.49079220129140566 1104 52-53 0.44447489936983686 1104 54-55 0.4789721010982717 1104 56-57 0.4041873780687979 1104 58-59 0.4773264331890985 1104 60-61 0.44647969482289795 1104 62-63 0.5033676432226102 1104 64-65 0.513793934380864 1104 66-67 0.44709435066560843 1104 68-69 0.4470791198662383 1104 70-71 0.5958774256637156 1104 72-73 0.4743951330878602 1104 74-75 0.5022375708948061 1104 76-77 0.455458613928279 1104 78-79 0.22823304594502858 1104 80-81 0.20992166379843624 1104 82-83 0.275248591847312 1104 84-85 0.15404243254588934 1104 86-87 0.21978947127662707 1104 88-89 0.4207792928539469 1104 90-91 0.3323079442875674 1104 92-93 0.44416081825015397 1104 94-95 0.4368422168901276 1104 96-97 0.24813782261709605 1104 98-99 0.5716472326350974 1104 100-101 0.7323547382220923 1105 1 0.02167324715914276 1105 2 0.09688903598996745 1105 3 0.03692087230086827 1105 4 0.1410930648971629 1105 5 0.054996143823551336 1105 6 0.1367000381802086 1105 7 0.14912705527719794 1105 8 0.15115892225601613 1105 9 0.15543941179441134 1105 10-11 0.2287022616468235 1105 12-13 0.2756437137548744 1105 14-15 0.2161334118647602 1105 16-17 0.2947414408859643 1105 18-19 0.32100424835775243 1105 20-21 0.3693547993367048 1105 22-23 0.2412323077960039 1105 24-25 0.28551951717413715 1105 26-27 0.27504567436796634 1105 28-29 0.3121012892074049 1105 30-31 0.3473519008455881 1105 32-33 0.35259416909024566 1105 34-35 0.24112304306359533 1105 36-37 0.3624364437943228 1105 38-39 0.36890958218143766 1105 40-41 0.30509008964738626 1105 42-43 0.21678427470122585 1105 44-45 0.21624688489648491 1105 46-47 0.20938623814331692 1105 48-49 0.21385710850902484 1105 50-51 0.24830193269850298 1105 52-53 0.28374824715605484 1105 54-55 0.1888304263853513 1105 56-57 0.09820789144986009 1105 58-59 0.08014790361837498 1105 60-61 0.061112573315426744 1105 62-63 0.09165400467833962 1105 64-65 0.13324715379145147 1105 66-67 0.11499922881807123 1105 68-69 -0.0064479307127882635 1105 70-71 0.043763114270838344 1105 72-73 0.023061520413044434 1105 74-75 -0.023628964602821156 1105 76-77 0.05939016311970846 1105 78-79 -0.005383758266955141 1105 80-81 0.13106951438718184 1105 82-83 0.07054448127666646 1105 84-85 0.02482416361291584 1105 86-87 0.09648777640254735 1105 88-89 9.62565157234252E-4 1105 90-91 0.17786453219207843 1105 92-93 0.24587014521935657 1105 94-95 0.3495365869447191 1105 96-97 0.21301330311480982 1105 98-99 0.2975355081872557 1105 100-101 0.8185589125554671 1106 1 0.21049518518176313 1106 2 0.18994968906045528 1106 3 0.23165014696127884 1106 4 0.21751006894574232 1106 5 0.24596061466996844 1106 6 0.19465247678930808 1106 7 0.16832471693673767 1106 8 0.21823013191207252 1106 9 0.19670090485127645 1106 10-11 0.28059128506806275 1106 12-13 0.3230632150602588 1106 14-15 0.4095005464517669 1106 16-17 0.4502666215587112 1106 18-19 0.4310183427237888 1106 20-21 0.43134636163569695 1106 22-23 0.3772973310658969 1106 24-25 0.35999263382630176 1106 26-27 0.2637536277525001 1106 28-29 0.230734703260147 1106 30-31 0.13007637017851437 1106 32-33 0.3079391499824382 1106 34-35 0.23479003765287842 1106 36-37 0.34569820171176247 1106 38-39 0.3867474685940877 1106 40-41 0.4599034575387222 1106 42-43 0.5556355365726517 1106 44-45 0.5867679034745734 1106 46-47 0.564119674898393 1106 48-49 0.7535365326264767 1106 50-51 0.5589952052400307 1106 52-53 0.34930833266817984 1106 54-55 0.2712635490914721 1106 56-57 0.43713009811678205 1106 58-59 0.36872531857920876 1106 60-61 0.43155791744498373 1106 62-63 0.4368619202256596 1106 64-65 0.4409465559633148 1106 66-67 0.40590126824445605 1106 68-69 0.3643048408638876 1106 70-71 0.37257095792742945 1106 72-73 0.4930566015738904 1106 74-75 0.24444067480843046 1106 76-77 0.3571951311472574 1106 78-79 0.34846996302405486 1106 80-81 0.28067789911607477 1106 82-83 0.2522861287094109 1106 84-85 0.3981806091840667 1106 86-87 0.3847341156510993 1106 88-89 0.45246470296040897 1106 90-91 0.48569163499582046 1106 92-93 0.5836548703319231 1106 94-95 0.5691249179447588 1106 96-97 0.6670079680661907 1106 98-99 0.7548574840471041 1106 100-101 1.2556403382620758 1107 1 0.017672851569962233 1107 2 -0.011586201949114638 1107 3 0.1029030655826162 1107 4 0.09985107102358626 1107 5 1.687846676148297E-4 1107 6 -0.008073890410742024 1107 7 -0.04034064304440932 1107 8 -0.03170399828521653 1107 9 0.03785078810642517 1107 10-11 0.00674159674469621 1107 12-13 0.07122742901395185 1107 14-15 0.08505910095768598 1107 16-17 0.07599493807650504 1107 18-19 0.13528033316999455 1107 20-21 0.07953146113658249 1107 22-23 0.07687346156308195 1107 24-25 -0.03479776096409637 1107 26-27 -0.050957027089729934 1107 28-29 0.06626824866211933 1107 30-31 0.009464477198200427 1107 32-33 0.11056242826361284 1107 34-35 0.09955611952422316 1107 36-37 0.1619102060816573 1107 38-39 -0.034140488478080044 1107 40-41 -0.019612372635002373 1107 42-43 -0.1142143016982331 1107 44-45 0.09641381312047059 1107 46-47 0.12598159465505177 1107 48-49 0.11907511198084109 1107 50-51 0.11557174273761461 1107 52-53 0.07249959072785828 1107 54-55 0.07615023160973777 1107 56-57 0.14688579076721453 1107 58-59 0.2901659393619411 1107 60-61 0.19720279887933856 1107 62-63 0.30507840160003497 1107 64-65 0.38994913720274127 1107 66-67 0.26790563814356716 1107 68-69 0.3447158041695886 1107 70-71 0.3953835985032228 1107 72-73 0.3156826110419999 1107 74-75 0.3905444492016841 1107 76-77 0.2951058802422182 1107 78-79 0.2674393951513814 1107 80-81 0.23173824228168272 1107 82-83 0.23378474705013375 1107 84-85 0.3835133320167863 1107 86-87 0.30922509737892057 1107 88-89 0.4184512505259015 1107 90-91 0.5561351047813936 1107 92-93 0.7318504126064127 1107 94-95 0.6098228165373918 1107 96-97 0.6246986691779455 1107 98-99 0.8757036700248726 1107 100-101 1.4221254613261465 1108 1 0.17894398476118312 1108 2 0.15911125942537296 1108 3 0.08220531058963587 1108 4 0.11629605572606039 1108 5 0.17234021089009843 1108 6 0.09541782073912941 1108 7 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pass #Quality Count 2 8053.0 3 1457.0 4 1258.0 5 1257.0 6 1537.0 7 1699.0 8 1991.0 9 2211.0 10 2633.0 11 3415.0 12 4800.0 13 6857.0 14 7465.0 15 8829.0 16 11669.0 17 16400.0 18 14825.0 19 9256.0 20 8515.0 21 8671.0 22 8950.0 23 9523.0 24 10632.0 25 12124.0 26 13988.0 27 16444.0 28 19606.0 29 24060.0 30 30295.0 31 38527.0 32 51163.0 33 70867.0 34 104954.0 35 169715.0 36 267276.0 37 426407.0 38 408650.0 39 69884.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27337368400806 19.04485593013862 14.47243843743722 23.209331948416107 2 14.982794902209065 20.173470945645132 41.25123713354423 23.592497018601577 3 19.050789611524053 24.975879432938417 29.93385064630305 26.03948030923448 4 12.421288298887735 16.63739555819381 37.35157145816029 33.58974468475817 5 13.364284707058708 37.33067903582127 34.316712459886425 14.988323797233605 6 31.073067431005043 36.156369705972494 17.877711555527554 14.892851307494908 7 27.669036864843154 33.1728571001252 21.028707316135154 18.129398718896493 8 25.763210553699572 34.97403348030423 19.899883869134296 19.362872096861903 9 26.224730795404504 16.9576878427605 20.720120625008338 36.097460736826655 10-11 24.502277750455097 26.781523388126434 27.366780567539266 21.349418293879204 12-13 25.60053060086703 24.360592846073416 28.004468667527505 22.034407885532048 14-15 22.576984829254034 25.431305895997575 26.994985792857324 24.996723481891063 16-17 21.44994982317881 28.894661806087697 27.38917014270737 22.266218228026133 18-19 21.16556585684238 27.159130846282935 30.269866652993336 21.405436643881345 20-21 22.085937070326665 26.192325990808364 30.39012937389103 21.33160756497395 22-23 21.616009243251515 26.327775933412696 30.274346264573115 21.781868558762675 24-25 21.45424051106197 26.471840314499673 30.41646636541358 21.657452809024775 26-27 21.30877766829536 26.61724631459188 30.422547485400315 21.651428531712448 28-29 21.006155785139207 26.885419656501096 30.469693301113942 21.638731257245755 30-31 21.276353571704345 26.648131072974145 30.384702631169024 21.69081272415248 32-33 21.055944761629153 26.783289666672182 30.339243772094733 21.82152179960393 34-35 21.568991797070193 26.801130085189516 30.06973599885997 21.56014211888032 36-37 21.46162100170849 26.664414710781465 30.14288419383724 21.731080093672805 38-39 21.23313954219082 26.928224542082518 30.198580433753087 21.640055481973572 40-41 21.397077692949253 26.888994033489443 30.144057013323067 21.569871260238234 42-43 21.211246093323837 26.9106347158849 29.881312985459996 21.99680620533126 44-45 21.305173923798446 27.08980658791124 29.705877240484835 21.89914224780548 46-47 21.443457829079517 27.21977091256558 29.318442017173936 22.018329241180968 48-49 21.572677445663004 27.30203845484112 29.179041060384904 21.946243039110975 50-51 21.390639363629287 27.55695837223814 29.08810704533802 21.964295218794557 52-53 21.562542961024956 27.66024027683349 28.887380999632505 21.889835762509048 54-55 21.368225194897377 27.405396977121537 29.051711157391818 22.174666670589264 56-57 21.464518911668893 27.77023574375077 28.78903871643821 21.97620662814213 58-59 21.639919330261527 27.463239343392814 28.899179936075864 21.9976613902698 60-61 21.630686432789577 27.373956491261076 29.242671286161855 21.752685789787492 62-63 21.458703538054035 27.47279371357286 29.250792555659405 21.8177101927137 64-65 21.296577418888006 27.510841102645333 29.100379074123957 22.0922024043427 66-67 21.28465312300449 27.76091381598536 28.729217623043684 22.225215437966465 68-69 21.274052545210466 27.52419634014471 28.994917551328776 22.206833563316053 70-71 21.36394365006518 27.473629607712574 28.90474288516396 22.257683857058282 72-73 21.391982781381486 27.446720803877934 28.962445018148724 22.198851396591852 74-75 21.178760946428373 27.7573176362081 28.787080365736028 22.2768410516275 76-77 21.293569275964515 27.640794781903555 28.702044510439723 22.36359143169221 78-79 21.333161134512146 27.564298478313244 28.76509112766965 22.33744925950496 80-81 21.295948159720346 27.767693207034334 28.592458963611577 22.34389966963374 82-83 21.328642808067784 27.728674472857474 28.605446825217047 22.3372358938577 84-85 21.36770949057297 27.566778688800795 28.497259531732144 22.568252288894083 86-87 21.252460366701612 27.58445476134443 28.703547206948933 22.459537665005026 88-89 21.391221879855074 27.450380462588804 28.501811045249788 22.656586612306334 90-91 21.319252715752125 27.465095606726404 28.64023403257592 22.575417644945553 92-93 21.407677663022675 27.418138570510276 28.63117690693013 22.543006859536916 94-95 21.296549945586282 27.328982019396324 28.58769383214893 22.786774202868465 96-97 21.288835835844942 27.229908790260403 28.64083202201832 22.840423351876336 98-99 21.32699139665283 27.176759191884603 28.426625433377563 23.069623978085005 100-101 21.509685467038818 26.715751039581665 28.80962416729161 22.96493932608791 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4176.0 1 2681.5 2 1409.0 3 1663.5 4 1363.5 5 1256.0 6 2178.0 7 4191.0 8 6378.5 9 6825.5 10 5135.5 11 3554.0 12 3275.0 13 3342.0 14 3398.0 15 3461.5 16 3485.0 17 3663.5 18 3806.0 19 3965.0 20 4382.0 21 4981.0 22 5781.5 23 6795.5 24 8096.5 25 9820.0 26 12304.0 27 15267.5 28 17878.0 29 20739.5 30 24266.5 31 27852.0 32 31485.0 33 34951.5 34 38948.0 35 42277.5 36 45391.5 37 49224.0 38 51306.0 39 52042.5 40 53656.0 41 56647.5 42 59054.5 43 61358.5 44 63769.5 45 64893.5 46 65066.5 47 66718.5 48 72432.5 49 72840.0 50 67739.5 51 64749.0 52 61922.5 53 60058.5 54 58292.5 55 54195.5 56 49006.0 57 43954.5 58 39382.5 59 34695.5 60 29418.5 61 24146.5 62 19360.0 63 15134.0 64 11615.5 65 8908.0 66 6614.5 67 5226.0 68 3866.0 69 2513.0 70 1838.0 71 1372.0 72 1073.5 73 883.5 74 711.0 75 561.0 76 364.5 77 219.0 78 163.5 79 123.0 80 89.0 81 71.5 82 51.5 83 41.5 84 44.0 85 34.0 86 22.0 87 17.5 88 13.5 89 12.5 90 10.5 91 7.0 92 4.5 93 4.0 94 3.5 95 3.5 96 4.0 97 4.5 98 3.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22943702635967025 2 0.13934674416918635 3 0.16061658002362603 4 0.13881366557634325 5 0.19787877366335876 6 0.1640815908771062 7 0.2004375509090056 8 0.20630141543027972 9 0.1237275413988835 10-11 0.17138476759905666 12-13 0.17602255135679162 14-15 0.14491741546439674 16-17 0.24063167680937533 18-19 0.19393399207631978 20-21 0.16562751879635118 22-23 0.20403583141069656 24-25 0.198092005100496 26-27 0.2920737610187345 28-29 0.26509998422087366 30-31 0.2420976429396939 32-33 0.15965703855650848 34-35 0.3076663098593952 36-37 0.3417033780124272 38-39 0.32515128770464885 40-41 0.29084768025519536 42-43 0.32091331289154623 44-45 0.3296291478845309 46-47 0.3337338530494228 48-49 0.3524982195174999 50-51 0.3455681978105396 52-53 0.3448485417102014 54-55 0.34045064331924585 56-57 0.3201403489319237 58-59 0.29607185046505774 60-61 0.2657130246026432 62-63 0.32802991210600163 64-65 0.3591083940687544 66-67 0.3653987214643029 68-69 0.3686238469510037 70-71 0.36763765155424394 72-73 0.36017455125444053 74-75 0.3560431921599065 76-77 0.34127691513815267 78-79 0.317954726701267 80-81 0.3280032581763595 82-83 0.3364525538729226 84-85 0.33717220997326075 86-87 0.3334939676826434 88-89 0.34093041405280466 90-91 0.3600679355358719 92-93 0.35964147266159746 94-95 0.3668646875946215 96-97 0.3681973840767292 98-99 0.3654786832532294 100-101 0.34085045226387817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1875896.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.39559196011981 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8749250170524 46.25427270237771 2 13.119696696477625 15.58504303249034 3 4.115369436899502 7.333044115176926 4 1.761128385714876 4.184130519492211 5 0.8682402462215429 2.5784821693964357 6 0.5120591930170423 1.8248435332721085 7 0.333369901951806 1.3860491867680231 8 0.23288211266534695 1.1065736750945276 9 0.1791763390751655 0.9578056252154941 >10 0.9008335243561405 9.896683868884475 >50 0.06763355265175806 2.765498418795664 >100 0.03261949323058706 3.4230445154604583 >500 9.881351107616093E-4 0.3817040753065918 >1k 8.084741815322252E-4 0.8327700876971319 >5k 1.7966092922938338E-4 0.83858581934494 >10k+ 8.983046461469169E-5 0.6514686552269336 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12210 0.6508889618614251 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9256 0.4934175455355734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6461 0.34442207883592696 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2920 0.1556589491101852 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2463 0.13129725741725554 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1970 0.10501648279009071 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 5.330785928431E-5 3 0.0 0.0 0.0 0.0 5.330785928431E-5 4 0.0 0.0 0.0 0.0 5.330785928431E-5 5 0.0 0.0 0.0 0.0 5.330785928431E-5 6 5.330785928431E-5 0.0 0.0 0.0 1.0661571856862E-4 7 5.330785928431E-5 0.0 0.0 0.0 1.0661571856862E-4 8 5.330785928431E-5 0.0 0.0 5.330785928431E-5 1.0661571856862E-4 9 5.330785928431E-5 0.0 0.0 1.5992357785293002E-4 1.5992357785293002E-4 10-11 5.330785928431E-5 0.0 0.0 1.5992357785293002E-4 2.1323143713724E-4 12-13 5.330785928431E-5 0.0 0.0 1.5992357785293002E-4 2.1323143713724E-4 14-15 5.330785928431E-5 0.0 0.0 1.5992357785293002E-4 2.9319322606370505E-4 16-17 7.996178892646501E-5 0.0 0.0 1.5992357785293002E-4 3.1984715570586005E-4 18-19 1.0661571856862E-4 0.0 0.0 1.5992357785293002E-4 3.7315501499017004E-4 20-21 1.0661571856862E-4 0.0 0.0 2.1323143713724E-4 3.7315501499017004E-4 22-23 1.0661571856862E-4 0.0 0.0 3.4650108534801504E-4 5.06424663200945E-4 24-25 1.0661571856862E-4 0.0 0.0 4.2646287427448E-4 6.396943114117201E-4 26-27 1.0661571856862E-4 0.0 0.0 6.66348241053875E-4 7.463100299803401E-4 28-29 1.5992357785293002E-4 0.0 0.0 0.00175915935638223 7.463100299803401E-4 30-31 1.5992357785293002E-4 0.0 0.0 0.0047977073355879 7.996178892646501E-4 32-33 1.5992357785293002E-4 0.0 0.0 0.010501648279009072 8.5292574854896E-4 34-35 1.5992357785293002E-4 5.330785928431E-5 0.0 0.02142975943229262 8.5292574854896E-4 36-37 1.5992357785293002E-4 5.330785928431E-5 0.0 0.039421161940747254 8.5292574854896E-4 38-39 1.8657750749508502E-4 5.330785928431E-5 0.0 0.06567528263826992 8.5292574854896E-4 40-41 2.1323143713724E-4 5.330785928431E-5 0.0 0.09646057137495895 9.5954146711758E-4 42-43 2.1323143713724E-4 5.330785928431E-5 0.0 0.12932486662373607 9.5954146711758E-4 44-45 2.1323143713724E-4 5.330785928431E-5 0.0 0.17205111584011054 9.5954146711758E-4 46-47 2.1323143713724E-4 5.330785928431E-5 0.0 0.2252257054762098 0.00101284932640189 48-49 2.1323143713724E-4 5.330785928431E-5 0.0 0.28511708538213204 0.00111946504497051 50-51 2.1323143713724E-4 5.330785928431E-5 0.0 0.3512721387539608 0.00111946504497051 52-53 2.1323143713724E-4 5.330785928431E-5 0.0 0.41718730675901006 0.00111946504497051 54-55 2.1323143713724E-4 5.330785928431E-5 0.0 0.4953899363290929 0.00111946504497051 56-57 2.1323143713724E-4 5.330785928431E-5 0.0 0.5933431277640125 0.00111946504497051 58-59 2.1323143713724E-4 5.330785928431E-5 0.0 0.7056094794167693 0.00111946504497051 60-61 2.1323143713724E-4 5.330785928431E-5 0.0 0.8326954159505644 0.0011727729042548202 62-63 2.1323143713724E-4 5.330785928431E-5 0.0 0.961247318614678 0.0011727729042548202 64-65 2.1323143713724E-4 5.330785928431E-5 0.0 1.1026197614366682 0.0011727729042548202 66-67 2.1323143713724E-4 5.330785928431E-5 0.0 1.2653420019020245 0.0011727729042548202 68-69 2.1323143713724E-4 5.330785928431E-5 0.0 1.4586896075262168 0.0011727729042548202 70-71 2.6653929642155006E-4 5.330785928431E-5 0.0 1.677971486692226 0.0011727729042548202 72-73 3.1984715570586005E-4 5.330785928431E-5 0.0 1.8997055273853136 0.0014393122006763702 74-75 3.1984715570586005E-4 5.330785928431E-5 0.0 2.1267970079364744 0.0014393122006763702 76-77 3.1984715570586005E-4 5.330785928431E-5 0.0 2.373932243578535 0.0014393122006763702 78-79 3.1984715570586005E-4 5.330785928431E-5 0.0 2.6613682208395346 0.0014393122006763702 80-81 3.7315501499017004E-4 5.330785928431E-5 0.0 2.9799359879225715 0.0014393122006763702 82-83 3.7315501499017004E-4 5.330785928431E-5 0.0 3.3081524775360682 0.0014926200599606801 84-85 3.7315501499017004E-4 5.330785928431E-5 0.0 3.6358625424863638 0.0014926200599606801 86-87 3.7315501499017004E-4 5.330785928431E-5 0.0 3.980711084196565 0.0014926200599606801 88-89 3.7315501499017004E-4 5.330785928431E-5 0.0 4.367059794359602 0.0014926200599606801 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6920 0.0 36.518173 1 GTATCAA 10625 0.0 32.904278 1 TATCAAC 12995 0.0 26.689096 2 ATCAACG 12990 0.0 26.626322 3 TCAACGC 13100 0.0 26.366524 4 CAACGCA 13300 0.0 25.970034 5 GTATACG 110 9.4782765E-5 25.909681 1 GTACATG 8710 0.0 25.52297 1 AACGCAG 13605 0.0 25.422707 6 TACATGG 9065 0.0 24.075914 2 ACATGGG 8990 0.0 23.274035 3 ACGCAGA 15205 0.0 22.686316 7 GAGTACT 8430 0.0 22.328117 12-13 CGCAGAG 15285 0.0 22.319242 8 GTGTAGC 1070 0.0 21.308897 1 CATGGGG 3545 0.0 21.146263 4 GCAGAGT 16020 0.0 20.939257 9 GTACTTT 9160 0.0 20.652065 14-15 AGTACTT 8895 0.0 20.12018 12-13 CATGGGA 5430 0.0 20.096516 4 >>END_MODULE