FastQCFastQC Report
Thu 26 May 2016
SRR938682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875896
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121700.6487566474900528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114100.6082426744339772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79030.421292011923902No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32490.1731972348147232No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29880.1592838835415183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29040.15480602336163626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23870.12724586011164798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101450.031.6345121
GTATCAA141500.026.7800461
TATCAAC172500.021.820442
ATCAACG172950.021.6813343
TCAACGC174800.021.479024
CAACGCA176950.021.2180445
GAGTACT109250.021.068912-13
AACGCAG181100.020.8082316
AGTACTT111350.020.71417212-13
GTACATG87800.020.4694771
GTACTTT113900.020.1254214-15
ATACCGT1200.00478753319.7752466
TACATGG91650.019.2141362
AGAGTAC165750.018.81193210-11
ACGCAGA201100.018.6433937
CGCAGAG201500.018.4415198
ACATGGG90550.018.1370683
TACTTTT127100.018.0352914-15
ACTTTTT131950.017.7318216-17
GCAGAGT207350.017.5779069
Produced by FastQC (version 0.11.5)