##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938682_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1875896 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.479102786081956 31.0 31.0 34.0 30.0 34.0 2 31.72203629625523 31.0 31.0 34.0 30.0 34.0 3 31.757857578458506 31.0 31.0 34.0 30.0 34.0 4 35.52407009770265 37.0 35.0 37.0 33.0 37.0 5 35.39782429303117 37.0 35.0 37.0 33.0 37.0 6 35.4577972339618 37.0 35.0 37.0 33.0 37.0 7 35.37618556679048 37.0 35.0 37.0 33.0 37.0 8 35.35153654573601 37.0 35.0 37.0 32.0 37.0 9 36.91524263605232 39.0 37.0 39.0 33.0 39.0 10-11 36.789955573230074 39.0 37.0 39.0 32.0 39.0 12-13 36.67768095885913 38.5 35.0 39.0 32.0 39.0 14-15 37.66279740454695 39.0 36.5 41.0 32.0 41.0 16-17 37.51736823363342 39.0 36.0 40.5 32.0 41.0 18-19 37.72370509879013 39.5 37.0 41.0 32.0 41.0 20-21 37.740693513926146 39.5 37.0 41.0 32.0 41.0 22-23 37.63438005091967 39.0 36.5 41.0 32.0 41.0 24-25 37.51638390401173 39.0 36.0 41.0 32.0 41.0 26-27 37.25494057239847 39.0 36.0 41.0 31.0 41.0 28-29 37.000085559114154 39.0 36.0 40.0 31.0 41.0 30-31 36.672745184167994 38.5 35.5 40.0 30.0 41.0 32-33 36.42564299939869 38.0 35.0 40.0 30.0 41.0 34-35 36.32808028803302 38.0 35.0 40.0 30.0 41.0 36-37 36.14432516514775 38.0 35.0 40.0 30.0 41.0 38-39 35.968608600903245 38.0 35.0 40.0 29.5 41.0 40-41 35.69478665128557 38.0 35.0 40.0 28.5 41.0 42-43 35.396755736991814 38.0 34.0 40.0 27.0 41.0 44-45 35.01469964219765 38.0 34.0 40.0 26.0 41.0 46-47 34.56205727822865 38.0 33.0 40.0 24.5 41.0 48-49 34.111596538400846 38.0 33.0 40.0 23.5 41.0 50-51 33.78426629194796 37.5 33.0 40.0 23.0 41.0 52-53 33.46935624362971 37.0 32.5 40.0 22.0 41.0 54-55 33.081119102551526 37.0 32.0 39.5 20.0 41.0 56-57 32.70509985628201 36.0 31.0 39.0 18.5 40.0 58-59 32.2889032760878 36.0 31.0 39.0 17.5 40.0 60-61 31.799621620814797 35.5 30.5 39.0 13.5 40.0 62-63 31.31817648739589 35.0 29.5 38.0 10.0 40.0 64-65 30.809881517951954 34.5 29.0 38.0 8.0 40.0 66-67 30.27536574522255 34.0 28.0 38.0 7.0 40.0 68-69 29.77045849023613 34.0 28.0 37.0 4.0 39.0 70-71 29.200115837978224 34.0 27.0 36.5 2.0 39.0 72-73 28.567253994890976 33.0 26.0 36.0 2.0 39.0 74-75 27.937995763091344 32.5 26.0 35.5 2.0 38.0 76-77 26.348768535142675 30.5 24.0 34.0 2.0 36.0 78-79 27.297755312661256 32.0 26.0 35.0 2.0 37.0 80-81 27.264034093574484 32.0 26.0 35.0 2.0 36.5 82-83 26.757575846422192 32.0 25.0 35.0 2.0 36.0 84-85 26.150104803251352 32.0 23.5 34.5 2.0 36.0 86-87 25.281138453304447 31.0 20.5 34.0 2.0 35.0 88-89 24.404783900600034 31.0 17.5 34.0 2.0 35.0 90-91 23.58578007522805 30.0 9.0 34.0 2.0 35.0 92-93 22.95681770204745 30.0 4.5 34.0 2.0 35.0 94-95 22.22330315753112 29.0 2.0 34.0 2.0 35.0 96-97 21.101028788376325 29.0 2.0 34.0 2.0 35.0 98-99 19.697342496598957 28.0 2.0 33.5 2.0 35.0 100-101 16.128217395847106 13.5 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0819693058794293 1101 2 0.06360241983328763 1101 3 -0.11758576308716684 1101 4 0.02742060021246573 1101 5 -0.06762684816244757 1101 6 0.09444262767282652 1101 7 0.10990440452381023 1101 8 0.19313735884575323 1101 9 0.2920711237711444 1101 10-11 0.24716005297991472 1101 12-13 0.15118551110099787 1101 14-15 0.2804815998629877 1101 16-17 0.2891851967033645 1101 18-19 0.35578164966752013 1101 20-21 0.2740111691925122 1101 22-23 0.09027238409450433 1101 24-25 0.251947799802565 1101 26-27 0.18180598640137902 1101 28-29 0.18579406578538737 1101 30-31 0.34593546849439605 1101 32-33 0.44827030427632764 1101 34-35 0.5545842250165336 1101 36-37 0.5642169189325301 1101 38-39 0.5986225230898796 1101 40-41 0.7438607373317865 1101 42-43 0.6800627843244911 1101 44-45 0.6544144962898386 1101 46-47 0.5946295640225827 1101 48-49 0.5389927410980704 1101 50-51 0.5721030704604715 1101 52-53 0.4919191023642 1101 54-55 0.47931359188379474 1101 56-57 0.5203169506356033 1101 58-59 0.6508146496084279 1101 60-61 0.6944231273020769 1101 62-63 0.5212300187500105 1101 64-65 0.6013732674988752 1101 66-67 0.8681993638964087 1101 68-69 0.8923810026830026 1101 70-71 0.8253317981214181 1101 72-73 0.7480299261926646 1101 74-75 0.6752136574197607 1101 76-77 0.7742843225316598 1101 78-79 0.6058719327397704 1101 80-81 0.7713400680212352 1101 82-83 0.7430098755535717 1101 84-85 0.7949473692550661 1101 86-87 0.9519121520578224 1101 88-89 1.071731555222211 1101 90-91 1.290956857976873 1101 92-93 1.2652250705041084 1101 94-95 1.3331093423437004 1101 96-97 1.265484780060504 1101 98-99 0.8056596605191295 1101 100-101 1.2515549272819406 1102 1 0.24391876238520993 1102 2 0.1263596176340478 1102 3 0.2177156045230113 1102 4 0.2574343921817288 1102 5 0.17241462118686712 1102 6 -0.019176194440674976 1102 7 0.15433538300609229 1102 8 0.1753026419548931 1102 9 0.3003649936332664 1102 10-11 0.29827817428196113 1102 12-13 0.29559588572676176 1102 14-15 0.4411858406132154 1102 16-17 0.4618249571940396 1102 18-19 0.32544212053805666 1102 20-21 0.3485156020585123 1102 22-23 0.3059788767855096 1102 24-25 0.34076046636208446 1102 26-27 0.3069314316502556 1102 28-29 0.3563963337981093 1102 30-31 0.4482917834596236 1102 32-33 0.46448918357603475 1102 34-35 0.5318816718202655 1102 36-37 0.5211681742259913 1102 38-39 0.6425068686441193 1102 40-41 0.7324751209727864 1102 42-43 0.5671384663541872 1102 44-45 0.6797217320278577 1102 46-47 0.5230325642431097 1102 48-49 0.47659435875776524 1102 50-51 0.5156155654613528 1102 52-53 0.32582305195062133 1102 54-55 0.5673833983103265 1102 56-57 0.4528837052618968 1102 58-59 0.49011227768872345 1102 60-61 0.39157376812406497 1102 62-63 0.39992773354404676 1102 64-65 0.6465389299806112 1102 66-67 0.8728758786769895 1102 68-69 1.0065990185830103 1102 70-71 0.8547072443047057 1102 72-73 0.9584973861155959 1102 74-75 0.9044752451034164 1102 76-77 0.743380640187997 1102 78-79 0.8976262787051503 1102 80-81 1.138269757010459 1102 82-83 0.957177853516324 1102 84-85 1.1853701267518808 1102 86-87 1.3180352070724588 1102 88-89 1.3877578048157382 1102 90-91 1.5156993798515366 1102 92-93 1.8522854748442832 1102 94-95 1.9608642321387642 1102 96-97 1.8016188518310727 1102 98-99 1.9859387329811646 1102 100-101 2.366368632911229 1103 1 0.13283821033395427 1103 2 0.13790739193776957 1103 3 0.15664250814599612 1103 4 0.00856398600132735 1103 5 0.11167170466911358 1103 6 -0.03529536215074103 1103 7 0.043192818680196865 1103 8 0.01425747477027528 1103 9 0.14640271697804508 1103 10-11 0.10901588366214554 1103 12-13 0.12054162498645127 1103 14-15 0.21486660047660422 1103 16-17 0.20313720319815332 1103 18-19 -0.06967879156309209 1103 20-21 0.10710051508748109 1103 22-23 0.19646957849463575 1103 24-25 0.259376338190755 1103 26-27 0.36507799837144006 1103 28-29 0.17740251845740573 1103 30-31 0.2642027840111041 1103 32-33 0.38129569344474845 1103 34-35 0.25821961031450513 1103 36-37 0.425231990063736 1103 38-39 0.3290590808491771 1103 40-41 0.536109111273305 1103 42-43 0.44944792653970467 1103 44-45 0.6576083205716827 1103 46-47 0.5301369933869822 1103 48-49 0.4454143740478145 1103 50-51 0.506619288564714 1103 52-53 0.6826550531245914 1103 54-55 0.4792084526894129 1103 56-57 0.3532377906163475 1103 58-59 0.6223061157514778 1103 60-61 0.6783642068727467 1103 62-63 0.7173193363246568 1103 64-65 0.7181973086199811 1103 66-67 0.689704282192281 1103 68-69 0.5585838847477405 1103 70-71 0.6863848656842642 1103 72-73 0.7224631657542666 1103 74-75 0.8597833301312434 1103 76-77 0.9341564326788294 1103 78-79 0.7868940131506044 1103 80-81 0.8993817885751803 1103 82-83 0.8578605802575758 1103 84-85 1.0063216364667404 1103 86-87 1.1305654735595567 1103 88-89 1.0522186175552406 1103 90-91 1.1163511004554678 1103 92-93 1.0770346446608734 1103 94-95 1.2812693474413557 1103 96-97 1.5403935650243028 1103 98-99 1.7141215366460436 1103 100-101 1.86178623350958 1104 1 0.04390585499573163 1104 2 -0.0319655290679961 1104 3 -0.011788775623511327 1104 4 0.029461688920221718 1104 5 -0.18412834966394342 1104 6 -0.08947629124609335 1104 7 -0.08718721090114201 1104 8 -0.07713314975808316 1104 9 -0.10304399134396647 1104 10-11 0.040425222911750325 1104 12-13 -0.12214484306269213 1104 14-15 -0.18188850834044956 1104 16-17 0.1660301368816377 1104 18-19 0.17454469843056586 1104 20-21 0.09372552640687104 1104 22-23 0.07175648724528116 1104 24-25 0.031033194721288737 1104 26-27 0.056450511879241105 1104 28-29 0.03693163192296112 1104 30-31 -0.2415951573695594 1104 32-33 -0.21062799878864524 1104 34-35 0.015286832385804416 1104 36-37 0.042890235105844 1104 38-39 0.09333509280245522 1104 40-41 0.2287524147234592 1104 42-43 0.22626219802390324 1104 44-45 0.14979583000449992 1104 46-47 0.1595915498179039 1104 48-49 0.4136011590398141 1104 50-51 0.36670803256543394 1104 52-53 0.3975356713141025 1104 54-55 0.2694991345693296 1104 56-57 0.2246459224645747 1104 58-59 0.6253807633412052 1104 60-61 0.908515433894383 1104 62-63 0.6759465209665123 1104 64-65 0.6246387949310694 1104 66-67 0.4907589472893221 1104 68-69 0.4193602438289048 1104 70-71 0.721127236416855 1104 72-73 0.7933641890269314 1104 74-75 0.6179921501982513 1104 76-77 0.4002670910144275 1104 78-79 0.39537238557355536 1104 80-81 0.6647233322378341 1104 82-83 0.5720336612440242 1104 84-85 0.67443959958063 1104 86-87 1.0766266284389658 1104 88-89 1.1133596118502673 1104 90-91 1.167825035678387 1104 92-93 1.5223641143098199 1104 94-95 1.3372867340210917 1104 96-97 1.2276403880494442 1104 98-99 1.5445039810301182 1104 100-101 2.257368955595968 1105 1 0.06207932605656197 1105 2 0.016512606388332074 1105 3 0.08765873550460057 1105 4 0.11342906669314345 1105 5 -0.08360476656289251 1105 6 0.03355371367274529 1105 7 0.143432987917123 1105 8 0.10533489296901877 1105 9 0.17912394146833321 1105 10-11 0.20643956648962813 1105 12-13 0.1593197564770037 1105 14-15 0.0733233357384151 1105 16-17 0.10491532145955063 1105 18-19 0.1312713020632188 1105 20-21 0.11612628427245397 1105 22-23 0.0017197477676518247 1105 24-25 0.014203234739994741 1105 26-27 0.1635126289233213 1105 28-29 0.2154388553293458 1105 30-31 0.22286264688493418 1105 32-33 0.22153805150497163 1105 34-35 0.3241992831215086 1105 36-37 0.3145358600716861 1105 38-39 0.4355988518886349 1105 40-41 0.3149884096953173 1105 42-43 0.32235955460330956 1105 44-45 0.33055855370541565 1105 46-47 0.4978631547561747 1105 48-49 0.5772623542570088 1105 50-51 0.34174364860449913 1105 52-53 0.4731026434346006 1105 54-55 0.6815710589285189 1105 56-57 0.5607443721193448 1105 58-59 0.593843595742392 1105 60-61 0.6758721954764333 1105 62-63 0.7208274668410652 1105 64-65 0.5221945569564177 1105 66-67 0.4512298198670983 1105 68-69 0.4333715267957565 1105 70-71 0.7307586878387653 1105 72-73 0.9894797635291255 1105 74-75 0.9439071453705026 1105 76-77 1.1479903972030563 1105 78-79 0.7783850227393323 1105 80-81 0.8001146835826205 1105 82-83 0.9504923035229673 1105 84-85 0.8542130900739089 1105 86-87 1.129564147036433 1105 88-89 1.0844794675643215 1105 90-91 0.9788896303599657 1105 92-93 1.3539531422621884 1105 94-95 1.4594597678517722 1105 96-97 1.4981431419921094 1105 98-99 1.4825989935701216 1105 100-101 1.598251067417575 1106 1 0.22499222476631076 1106 2 0.10889691907515342 1106 3 0.12822496956567164 1106 4 0.07870395412739839 1106 5 0.21162963504140464 1106 6 0.1709690528834642 1106 7 0.08040563160398051 1106 8 0.2601695442989964 1106 9 0.3220114892378305 1106 10-11 0.23362797694783666 1106 12-13 0.2301983368243512 1106 14-15 0.25732339249425706 1106 16-17 0.33816289041789815 1106 18-19 0.16655924333984728 1106 20-21 0.3470468639387292 1106 22-23 0.3050465857108122 1106 24-25 0.24501030273348334 1106 26-27 0.24184987210316677 1106 28-29 0.33125386913992827 1106 30-31 0.35934151479517595 1106 32-33 0.4303711515087443 1106 34-35 0.4602241043406181 1106 36-37 0.24436909250575667 1106 38-39 0.5703537612684855 1106 40-41 0.7434240296582288 1106 42-43 0.5761527363354944 1106 44-45 0.5117302779477271 1106 46-47 0.5955352195335024 1106 48-49 0.5115934867251326 1106 50-51 0.5588181690176768 1106 52-53 0.6407191820853342 1106 54-55 0.6689570742904323 1106 56-57 0.4284275988778319 1106 58-59 0.6049251727978984 1106 60-61 0.7225160336844603 1106 62-63 0.8733209399198785 1106 64-65 0.8521304228612969 1106 66-67 0.8320591172298464 1106 68-69 0.7709694306135333 1106 70-71 1.0341044443940604 1106 72-73 0.9404450857130868 1106 74-75 1.0906848327593544 1106 76-77 1.1836708996813918 1106 78-79 1.040199696014902 1106 80-81 1.19883389354138 1106 82-83 1.4611458469790186 1106 84-85 1.4939932558009552 1106 86-87 1.5551832586184062 1106 88-89 1.726622094981174 1106 90-91 1.5728843899044094 1106 92-93 1.7573145066724898 1106 94-95 1.9609259602918954 1106 96-97 2.0779584017446524 1106 98-99 2.4057634152281473 1106 100-101 2.3298858246917824 1107 1 -0.017787266697389725 1107 2 -0.014046657815789843 1107 3 -0.05556302606442998 1107 4 -0.14912737633550677 1107 5 -0.04359924913484292 1107 6 -0.12725406902386993 1107 7 0.0023718719912793063 1107 8 -0.083764543056148 1107 9 -0.11934028764026294 1107 10-11 -0.2658887100688574 1107 12-13 -0.09237411995862743 1107 14-15 -0.09344053655915019 1107 16-17 -0.13792048040232174 1107 18-19 -0.04968810580223959 1107 20-21 -0.056803574122227474 1107 22-23 0.06877588759800801 1107 24-25 -0.054345993991233854 1107 26-27 0.01863746073285455 1107 28-29 0.1012702562615786 1107 30-31 0.04859884615777332 1107 32-33 -0.04092782242179993 1107 34-35 0.17394644437874973 1107 36-37 0.023842616058232124 1107 38-39 0.14307054253790596 1107 40-41 -0.1462740403029983 1107 42-43 -0.24611875435704889 1107 44-45 0.05695632383166327 1107 46-47 -0.16377705688404376 1107 48-49 -0.03336533126000063 1107 50-51 -0.14641366055625582 1107 52-53 -0.19345198300688793 1107 54-55 -0.09737917230896898 1107 56-57 -0.11387259605394462 1107 58-59 -0.0986580373642667 1107 60-61 -0.09931525393630025 1107 62-63 -0.0852175001975084 1107 64-65 -0.10754815392254358 1107 66-67 -0.08255674935970703 1107 68-69 0.05828440608640051 1107 70-71 -0.10919022390060462 1107 72-73 0.07891974458248541 1107 74-75 0.2420662242723246 1107 76-77 0.14005545080278736 1107 78-79 0.47396145082928953 1107 80-81 0.5029949371761049 1107 82-83 0.4335504160941106 1107 84-85 0.46149427330196957 1107 86-87 0.4314238065694802 1107 88-89 0.3266400351306906 1107 90-91 0.6226045771245943 1107 92-93 0.7961560014350404 1107 94-95 0.9105671573015108 1107 96-97 1.0471289242046495 1107 98-99 1.4171318469913174 1107 100-101 1.8337710719980826 1108 1 0.18041343916147312 1108 2 0.1427015760689585 1108 3 0.09438324175087232 1108 4 -0.03895995506930916 1108 5 -0.04173594616166554 1108 6 0.10601760442538222 1108 7 -0.0023330707824769092 1108 8 0.08154266997581772 1108 9 0.09566772814466873 1108 10-11 0.16472483513958736 1108 12-13 0.15164751385212583 1108 14-15 0.17347453135481317 1108 16-17 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-2.8428106434087397 2316 92-93 -2.6921320010261915 2316 94-95 -2.372835077675525 2316 96-97 -2.0730565333823847 2316 98-99 -2.191037965115502 2316 100-101 -1.9336506284944832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 15.0 4 50.0 5 111.0 6 262.0 7 587.0 8 1446.0 9 2549.0 10 4054.0 11 5856.0 12 7949.0 13 10710.0 14 13456.0 15 17173.0 16 21685.0 17 27516.0 18 26508.0 19 21014.0 20 20656.0 21 21857.0 22 23698.0 23 26292.0 24 30092.0 25 35049.0 26 40992.0 27 48354.0 28 57499.0 29 67408.0 30 79276.0 31 93539.0 32 111292.0 33 135097.0 34 167684.0 35 210357.0 36 241788.0 37 220510.0 38 80777.0 39 2728.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.178393327241096 22.34420339587754 16.07639469345398 20.40100858342738 2 17.920396439887927 23.171753657985303 37.58721165778913 21.32063824433764 3 21.30405950010022 26.100860602080285 30.433030402538307 22.162049495281188 4 15.177920311147314 19.435032645733024 35.52377104061206 29.8632760025076 5 13.302718052565297 38.50487381193836 34.104313758108354 14.088094377387986 6 30.847768813392346 37.83869223986549 16.663556912237752 14.649982034504413 7 27.18242375909965 34.09144216950193 20.79736829760285 17.92876577379556 8 25.93171476457117 36.258246725831285 19.17707591465625 18.632962594941297 9 26.034865472286313 17.045081390439556 20.341106329988442 36.57894680728569 10-11 25.02756016324999 27.577115149240683 26.777923722850304 20.617400964659023 12-13 26.06594395424906 25.01380673555464 27.678453389740156 21.241795920456145 14-15 22.69590105208391 26.22445487383096 26.777417298187107 24.302226775898024 16-17 21.316877539510003 29.492833664027156 27.46205195073817 21.728236845724666 18-19 21.163993099830694 27.404717532315225 30.64495579712308 20.786333570731 20-21 22.11295295688034 26.28269371009907 31.02834592109584 20.576007411924753 22-23 21.814908715621762 26.459862380430472 30.86866222860969 20.85656667533808 24-25 21.580432557000233 26.7212416069906 31.01709240646381 20.681233429545365 26-27 21.523506072093213 26.75202052160136 30.937023056415747 20.787450349889678 28-29 21.186622286096885 26.98963588599794 31.059317787340024 20.76442404056515 30-31 21.445218711485072 26.738022790176004 30.933084776554775 20.88367372178415 32-33 21.338389761479316 26.921295210395456 30.84952470712662 20.890790320998605 34-35 21.735559748179558 26.828078544350326 30.71488622605731 20.72147548141281 36-37 21.73717396931504 26.754742924899876 30.666511339380616 20.841571766404467 38-39 21.447793075081485 27.02375757427124 30.72394452025417 20.804504830393103 40-41 21.69210158185874 26.83929995607133 30.60325157482162 20.865346887248318 42-43 21.539537612530786 26.987876143300504 30.27637531257255 21.196210931596156 44-45 21.764079337510676 27.08605878771789 30.010757881560952 21.139103993210487 46-47 21.91379660523624 27.328242769161715 29.514193003957057 21.24376762164499 48-49 22.03626376496841 27.49901778750839 29.22792500747654 21.236793440046657 50-51 21.792812607948413 27.68431725426143 29.137622767999932 21.38524736979022 52-53 22.04567843846354 27.806818714896774 28.83784602131462 21.30965682532507 54-55 21.87192147112633 27.63314170934849 28.974127563574953 21.520809255950223 56-57 21.928343155364 27.876628897826894 28.78412056176152 21.41090738504759 58-59 22.193142660969098 27.62126523545116 28.76634959016743 21.419242513412314 60-61 22.02210934942837 27.66944512857583 29.123126590605473 21.185318931390327 62-63 21.883279438685378 27.59480606989139 29.15815067259028 21.363763818832954 64-65 21.75742344000676 27.628420054636116 29.018764938104567 21.595391567252555 66-67 21.809597835161355 27.785934306359213 28.68048691810099 21.723980940378443 68-69 21.75725280852756 27.633357748358918 28.850324165437918 21.759065277675603 70-71 21.831167612703474 27.581699625139134 28.809459586245715 21.777673175911673 72-73 21.929627228801596 27.557071394149784 28.701058054391076 21.812243322657547 74-75 21.697695041368352 27.86897742698734 28.420635426214908 22.012692105429394 76-77 21.734677088386125 27.697414579082764 28.595550546948274 21.972357785582837 78-79 21.736408273023216 27.60979252323579 28.661267534591474 21.992531669149525 80-81 21.615154036792273 27.791578685877532 28.505828988268455 22.087438289061737 82-83 21.71523673409987 27.72987732164247 28.545867248508834 22.009018695748825 84-85 21.633262185110475 27.598838101899037 28.432439751457437 22.33545996153305 86-87 21.66600107934521 27.514792978333862 28.473452136001765 22.34575380631916 88-89 21.69213480201913 27.412993414763985 28.383305708799895 22.51156607441699 90-91 21.68697770377142 27.36443098161068 28.488710137260636 22.459881177357264 92-93 21.62199189611763 27.302625792223534 28.48513053263756 22.590251779021276 94-95 21.48106074336902 27.243172847903825 28.475413296714308 22.800353112012843 96-97 21.47842575326751 27.145377084206253 28.431963639736573 22.944233522789666 98-99 21.460091604225397 27.06805174700516 28.296744595649226 23.175112053120213 100-101 21.826996606652944 26.207668788430365 28.9164558541034 23.048878750813287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3099.0 1 1988.0 2 1084.5 3 1381.5 4 1155.5 5 1024.0 6 1924.5 7 4208.5 8 6111.0 9 6416.5 10 5144.0 11 3431.0 12 2980.0 13 3100.5 14 3270.0 15 3449.0 16 3631.5 17 3889.5 18 4219.5 19 4502.5 20 4887.5 21 5510.0 22 6361.0 23 7484.5 24 8745.5 25 10558.0 26 13259.5 27 16106.0 28 18714.5 29 21773.0 30 25164.5 31 28568.0 32 32518.0 33 36326.0 34 40017.5 35 43004.0 36 46520.5 37 50385.0 38 52244.0 39 52991.5 40 54287.0 41 56947.0 42 59522.0 43 61397.0 44 63277.0 45 64932.5 46 65045.0 47 66092.0 48 70926.5 49 71826.0 50 67762.0 51 64312.5 52 60885.5 53 58569.0 54 56660.0 55 52343.5 56 47222.0 57 43183.0 58 38773.0 59 33965.0 60 29022.5 61 23681.5 62 19105.5 63 15112.5 64 11653.5 65 8915.5 66 6580.0 67 5188.5 68 3787.0 69 2473.5 70 1760.0 71 1266.5 72 971.5 73 788.0 74 622.5 75 489.0 76 353.5 77 219.0 78 135.5 79 91.5 80 59.0 81 38.5 82 23.0 83 11.0 84 7.0 85 4.5 86 2.0 87 2.5 88 1.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1901491340671338 2 0.0 3 0.0 4 0.0 5 0.01929744506092022 6 0.0041580130241761805 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.00223893008994102 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.39885366779395E-4 26-27 0.0010128493264018902 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00613040381769565 36-37 0.015272701684954816 38-39 0.011754382972190357 40-41 0.00682340598839168 42-43 0.007009983495886765 44-45 0.0033050872756272202 46-47 3.7315501499017004E-4 48-49 0.0015459279192449903 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.8638645212741E-4 58-59 4.2646287427448E-4 60-61 0.0020256986528037805 62-63 7.463100299803401E-4 64-65 0.0016258897081714552 66-67 0.00522417020986238 68-69 1.0661571856862E-4 70-71 0.0 72-73 0.0 74-75 2.93193226063705E-4 76-77 0.048057035144805464 78-79 0.12713924439307936 80-81 6.9300217069603E-4 82-83 7.996178892646501E-4 84-85 0.0 86-87 0.08590561523666557 88-89 0.31342355866210064 90-91 0.34047729724888803 92-93 2.6653929642155E-4 94-95 2.1323143713724E-4 96-97 1.0661571856862E-4 98-99 0.0 100-101 0.0010395032560440451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1875896.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.03525896662217 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4193457942613 47.07925732751571 2 12.987069975069462 15.59364218341877 3 3.9263221597102738 7.0715330293378065 4 1.6682582034121123 4.006172530601521 5 0.8573765813371629 2.573641254624689 6 0.4797933940436503 1.7282712397131088 7 0.3193204157270985 1.3419338696054068 8 0.2185182126890071 1.0495037990166372 9 0.15309518448556073 0.8271998142420092 >10 0.8707948140060133 9.614337282146822 >50 0.06649947713460667 2.738319775150577 >100 0.031028281751337292 3.21695826287565 >500 0.0015998315414854163 0.58908397882909 >1k 7.110362406601843E-4 0.890242491194251 >5k 8.887953008252304E-5 0.4216966199897007 >10k+ 1.7775906016504609E-4 1.2582065417382187 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12170 0.6487566474900528 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11410 0.6082426744339772 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7903 0.421292011923902 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3249 0.1731972348147232 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2988 0.1592838835415183 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2904 0.15480602336163626 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2387 0.12724586011164798 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.330785928431E-5 0.0 5 0.0 0.0 0.0 5.330785928431E-5 0.0 6 0.0 0.0 0.0 5.330785928431E-5 0.0 7 5.330785928431E-5 0.0 0.0 5.330785928431E-5 0.0 8 5.330785928431E-5 0.0 0.0 5.330785928431E-5 0.0 9 5.330785928431E-5 0.0 0.0 1.0661571856862E-4 0.0 10-11 5.330785928431E-5 0.0 0.0 1.0661571856862E-4 0.0 12-13 5.330785928431E-5 0.0 0.0 1.0661571856862E-4 0.0 14-15 5.330785928431E-5 0.0 0.0 1.0661571856862E-4 2.6653929642155E-5 16-17 5.330785928431E-5 0.0 0.0 1.5992357785293002E-4 1.5992357785293002E-4 18-19 5.330785928431E-5 0.0 0.0 1.5992357785293002E-4 2.1323143713724E-4 20-21 5.330785928431E-5 0.0 0.0 2.6653929642155006E-4 2.1323143713724E-4 22-23 5.330785928431E-5 0.0 0.0 4.2646287427448E-4 2.1323143713724E-4 24-25 5.330785928431E-5 0.0 0.0 4.7977073355879E-4 2.39885366779395E-4 26-27 5.330785928431E-5 0.0 0.0 7.463100299803401E-4 2.6653929642155E-4 28-29 7.996178892646501E-5 0.0 0.0 0.0018657750749508503 2.6653929642155E-4 30-31 1.0661571856862E-4 0.0 0.0 0.004851015194872211 2.6653929642155E-4 32-33 1.0661571856862E-4 0.0 0.0 0.010501648279009072 2.6653929642155E-4 34-35 1.0661571856862E-4 0.0 0.0 0.02113656620622892 2.6653929642155E-4 36-37 1.0661571856862E-4 0.0 0.0 0.03902135299611492 2.6653929642155E-4 38-39 1.0661571856862E-4 0.0 0.0 0.06500893439721606 2.6653929642155E-4 40-41 1.3326964821077503E-4 0.0 0.0 0.09510122096320905 2.6653929642155E-4 42-43 2.3988536677939503E-4 0.0 0.0 0.12721920618200583 2.6653929642155E-4 44-45 3.1984715570586005E-4 0.0 0.0 0.16890595214233625 2.9319322606370505E-4 46-47 3.4650108534801504E-4 0.0 0.0 0.22077449922596987 3.4650108534801504E-4 48-49 3.7315501499017004E-4 0.0 0.0 0.27893337370515214 4.2646287427448E-4 50-51 3.7315501499017004E-4 0.0 0.0 0.3434358834391672 4.2646287427448E-4 52-53 3.7315501499017004E-4 0.0 0.0 0.4072986988617706 4.7977073355879E-4 54-55 3.7315501499017004E-4 0.0 0.0 0.48320909048262806 5.330785928431E-4 56-57 3.7315501499017004E-4 0.0 0.0 0.5783902732347636 5.330785928431E-4 58-59 3.7315501499017004E-4 0.0 0.0 0.686631881511555 5.330785928431E-4 60-61 3.7315501499017004E-4 0.0 0.0 0.8093199196543945 5.330785928431E-4 62-63 3.7315501499017004E-4 0.0 0.0 0.9338470789425426 5.330785928431E-4 64-65 3.7315501499017004E-4 0.0 0.0 1.069755466187891 5.330785928431E-4 66-67 4.2646287427448E-4 0.0 0.0 1.2266938039208997 5.330785928431E-4 68-69 4.2646287427448E-4 0.0 0.0 1.411432190270676 5.330785928431E-4 70-71 4.2646287427448E-4 0.0 0.0 1.6211186547655094 6.396943114117201E-4 72-73 4.2646287427448E-4 0.0 0.0 1.8325109707574407 7.996178892646501E-4 74-75 4.53116803916635E-4 0.0 0.0 2.0504334995117 7.996178892646501E-4 76-77 4.7977073355879E-4 0.0 0.0 2.2885063990754286 7.996178892646501E-4 78-79 4.7977073355879E-4 0.0 0.0 2.564347916942091 7.996178892646501E-4 80-81 4.7977073355879E-4 0.0 0.0 2.868122753073731 8.262718189068051E-4 82-83 4.7977073355879E-4 0.0 0.0 3.178987534490185 8.5292574854896E-4 84-85 4.7977073355879E-4 0.0 0.0 3.4867871139977913 8.5292574854896E-4 86-87 4.7977073355879E-4 0.0 0.0 3.8059679214625968 8.79579678191115E-4 88-89 4.7977073355879E-4 0.0 0.0 4.160998264296102 9.5954146711758E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10145 0.0 31.634512 1 GTATCAA 14150 0.0 26.780046 1 TATCAAC 17250 0.0 21.82044 2 ATCAACG 17295 0.0 21.681334 3 TCAACGC 17480 0.0 21.47902 4 CAACGCA 17695 0.0 21.218044 5 GAGTACT 10925 0.0 21.0689 12-13 AACGCAG 18110 0.0 20.808231 6 AGTACTT 11135 0.0 20.714172 12-13 GTACATG 8780 0.0 20.469477 1 GTACTTT 11390 0.0 20.12542 14-15 ATACCGT 120 0.004787533 19.775246 6 TACATGG 9165 0.0 19.214136 2 AGAGTAC 16575 0.0 18.811932 10-11 ACGCAGA 20110 0.0 18.643393 7 CGCAGAG 20150 0.0 18.441519 8 ACATGGG 9055 0.0 18.137068 3 TACTTTT 12710 0.0 18.03529 14-15 ACTTTTT 13195 0.0 17.73182 16-17 GCAGAGT 20735 0.0 17.577906 9 >>END_MODULE