FastQCFastQC Report
Thu 26 May 2016
SRR938681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114722
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177550.570034821727268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144560.46411846707346593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92040.29549988730936505No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44150.1417461975739729No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41260.1324676809037853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36490.11715331255887362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA228850.028.6714821
GGTATCA159650.027.5280691
GTACATG156450.024.3941251
TATCAAC274300.023.6685262
ATCAACG274250.023.6555613
TCAACGC276050.023.5356464
TACATGG158900.023.3514772
CAACGCA278300.023.3113125
AACGCAG281450.023.0483536
TACCTGG22450.023.008412
GAGTACT146600.022.88555112-13
ACATGGG157800.022.733453
GTACTTT158650.021.01367414-15
ACGCAGA306900.020.983777
CGCAGAG308750.020.796698
GTACGCT1150.00377556820.603863
AGTACTT154700.020.4628712-13
GCAGAGT318150.019.6910729
GTATAGG9950.019.5355551
CATGGGG69100.018.99674
Produced by FastQC (version 0.11.5)