##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938681_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3114722 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4877671265686 33.0 31.0 34.0 30.0 34.0 2 31.550057436907693 33.0 31.0 34.0 28.0 34.0 3 31.551874292472974 33.0 31.0 34.0 28.0 34.0 4 35.10795024403462 37.0 35.0 37.0 32.0 37.0 5 35.143163017437836 37.0 35.0 37.0 32.0 37.0 6 35.228168677654054 37.0 35.0 37.0 32.0 37.0 7 35.14028699832601 37.0 35.0 37.0 32.0 37.0 8 35.11392702141636 37.0 35.0 37.0 32.0 37.0 9 36.656071071511356 39.0 37.0 39.0 32.0 39.0 10-11 36.54292951345256 39.0 37.0 39.0 32.0 39.0 12-13 36.413504640221504 39.0 37.0 39.0 32.0 39.0 14-15 37.534867317211614 40.0 37.0 41.0 32.0 41.0 16-17 37.466405027479176 40.0 37.0 41.0 32.0 41.0 18-19 37.42356685444159 40.0 37.0 41.0 32.0 41.0 20-21 37.32931943846032 40.0 37.0 41.0 31.5 41.0 22-23 37.242060126072246 40.0 37.0 41.0 31.0 41.0 24-25 37.244171710990585 40.0 37.0 41.0 31.0 41.0 26-27 37.140455231638654 40.0 37.0 41.0 31.0 41.0 28-29 37.00694460051331 40.0 36.5 41.0 30.5 41.0 30-31 36.77679147609321 39.5 36.0 41.0 30.0 41.0 32-33 36.53757526353877 39.0 35.5 41.0 30.0 41.0 34-35 36.196272733168485 39.0 35.0 41.0 29.5 41.0 36-37 35.93130751315847 39.0 35.0 41.0 28.5 41.0 38-39 35.74712879672728 39.0 35.0 41.0 27.0 41.0 40-41 35.55178696525725 39.0 35.0 40.5 27.0 41.0 42-43 35.26638412673748 38.0 35.0 40.0 25.5 41.0 44-45 35.1646986472629 39.0 35.0 40.5 25.0 41.0 46-47 34.91870398064418 39.0 35.0 41.0 24.0 41.0 48-49 34.64683621844903 39.0 34.0 41.0 23.0 41.0 50-51 33.49264059521202 38.0 32.5 40.0 21.0 40.5 52-53 33.58598664664134 38.0 33.0 39.5 20.5 40.5 54-55 34.0921655287374 38.0 34.0 40.0 21.0 41.0 56-57 33.99295811953684 38.0 34.0 40.0 20.0 41.0 58-59 33.772072595884964 38.0 33.5 40.0 19.0 41.0 60-61 33.48140957684185 37.5 33.0 40.0 18.0 41.0 62-63 33.12505048604659 37.0 33.0 40.0 15.0 41.0 64-65 32.732876160376435 36.5 32.0 39.5 12.5 41.0 66-67 32.336959606667946 36.0 32.0 39.0 8.0 41.0 68-69 31.936523548490044 35.5 31.0 39.0 7.5 40.5 70-71 31.528676556045774 35.0 31.0 38.5 6.5 40.0 72-73 31.11568512374459 35.0 31.0 37.5 2.0 40.0 74-75 30.67000778881711 35.0 30.5 37.0 2.0 39.0 76-77 30.240155301179367 35.0 30.0 36.5 2.0 39.0 78-79 29.82619090885158 34.5 30.0 36.0 2.0 38.5 80-81 29.39683734214482 34.0 29.0 35.5 2.0 37.5 82-83 28.951302395526792 34.0 29.0 35.0 2.0 37.0 84-85 28.557799861432258 34.0 29.0 35.0 2.0 36.5 86-87 28.182102447666274 34.0 28.0 35.0 2.0 36.0 88-89 27.810759676144453 34.0 27.0 35.0 2.0 36.0 90-91 27.406571758250017 34.0 26.5 35.0 2.0 35.5 92-93 26.967621990020298 34.0 25.5 35.0 2.0 35.0 94-95 26.479116434789365 33.0 25.0 35.0 2.0 35.0 96-97 25.9137465558724 33.0 23.5 35.0 2.0 35.0 98-99 25.227263620958787 33.0 19.0 35.0 2.0 35.0 100-101 23.03341614436216 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22576899733424938 1101 2 0.15417650956581141 1101 3 0.32898731762443845 1101 4 0.21173524419966583 1101 5 0.07631898990250363 1101 6 -0.00406116846642135 1101 7 0.18980502708271274 1101 8 0.3347370907998197 1101 9 0.37511041774665443 1101 10-11 0.3501629864258007 1101 12-13 0.39169354726992367 1101 14-15 0.5809257244996431 1101 16-17 0.5399681691090024 1101 18-19 0.6298431485272289 1101 20-21 0.6112770322250114 1101 22-23 0.5646843517849192 1101 24-25 0.5603000987158566 1101 26-27 0.42326442408547393 1101 28-29 0.4691217620083563 1101 30-31 0.4432109703599352 1101 32-33 0.5836766549814385 1101 34-35 0.5401486932579687 1101 36-37 0.6490621563550718 1101 38-39 0.4527850804909903 1101 40-41 0.40804848047160647 1101 42-43 0.5253041024852934 1101 44-45 0.7593224043613702 1101 46-47 0.7612592321766982 1101 48-49 0.873427569916096 1101 50-51 0.8677336624475558 1101 52-53 0.7882562733306031 1101 54-55 0.7810719124918677 1101 56-57 0.7735281952202371 1101 58-59 0.7740844895065919 1101 60-61 0.5850927594571402 1101 62-63 0.5919978324635267 1101 64-65 0.6798104631997361 1101 66-67 0.690533678145151 1101 68-69 0.6358218563336457 1101 70-71 0.47766096816623005 1101 72-73 0.48695216034608535 1101 74-75 0.5562706070857715 1101 76-77 0.521511306673542 1101 78-79 0.3158285190869101 1101 80-81 0.23369872007390669 1101 82-83 0.12723058737017112 1101 84-85 0.14930498678972626 1101 86-87 0.17239788844134551 1101 88-89 0.18254788424825463 1101 90-91 0.07316681147601756 1101 92-93 -0.03714007609916692 1101 94-95 -0.14694892865414388 1101 96-97 -0.1529429128851838 1101 98-99 -0.2430222412049048 1101 100-101 -0.032183868509747526 1102 1 0.26090930841124305 1102 2 0.27587094358237607 1102 3 0.2792933169461236 1102 4 0.2798915314683086 1102 5 0.15232790388211015 1102 6 0.09523784378505695 1102 7 0.24924496386582717 1102 8 0.22549003332029116 1102 9 0.29860178005072413 1102 10-11 0.21914282000803098 1102 12-13 0.2906310438272044 1102 14-15 0.2652754630865033 1102 16-17 0.3636619154454621 1102 18-19 0.3627616439760075 1102 20-21 0.416795262926108 1102 22-23 0.3629641740551506 1102 24-25 0.363182256267919 1102 26-27 0.3645834910601664 1102 28-29 0.408617468691304 1102 30-31 0.3922667440270402 1102 32-33 0.32632275925936227 1102 34-35 0.45741407638406173 1102 36-37 0.5112334070297351 1102 38-39 0.5056294889460986 1102 40-41 0.5167351305887422 1102 42-43 0.5173126466612104 1102 44-45 0.6479338556301073 1102 46-47 0.47500123728450916 1102 48-49 0.441473740803481 1102 50-51 0.5930342730411056 1102 52-53 0.5271016060913141 1102 54-55 0.43602979183059176 1102 56-57 0.39672775675035155 1102 58-59 0.3967053163986449 1102 60-61 0.41840786733300916 1102 62-63 0.35537259460348736 1102 64-65 0.3990608837343501 1102 66-67 0.40687077457726417 1102 68-69 0.38278571402968353 1102 70-71 0.31640828054761627 1102 72-73 0.2684828890273643 1102 74-75 0.24051949474741008 1102 76-77 0.34255742349546736 1102 78-79 0.17437911463682454 1102 80-81 0.12519987362318474 1102 82-83 0.1234498231288832 1102 84-85 0.026905818114951074 1102 86-87 -0.10023937699880037 1102 88-89 -0.14189412402610202 1102 90-91 -0.055382430910039915 1102 92-93 -0.050132129086986055 1102 94-95 -0.018562340839562097 1102 96-97 -0.03180374441200584 1102 98-99 -0.03913415040099366 1102 100-101 0.05658642932986169 1103 1 0.10371059945136096 1103 2 0.2366891656268031 1103 3 0.20121387152656922 1103 4 0.20649743292128875 1103 5 0.18097121412611727 1103 6 0.1815621534349674 1103 7 0.06802006863094334 1103 8 0.22738339576745403 1103 9 0.23032136713943174 1103 10-11 0.20296600726484115 1103 12-13 0.28335686517536374 1103 14-15 0.3716870717744811 1103 16-17 0.35672010867253334 1103 18-19 0.4791289957084359 1103 20-21 0.3460442712683047 1103 22-23 0.41378604274011366 1103 24-25 0.2958136550961754 1103 26-27 0.37400537725061866 1103 28-29 0.374911159902112 1103 30-31 0.3738378174350814 1103 32-33 0.3550604697876665 1103 34-35 0.4534873681987932 1103 36-37 0.5469362211891351 1103 38-39 0.4226845376532111 1103 40-41 0.5752666927274817 1103 42-43 0.4921629049682821 1103 44-45 0.5748690462974579 1103 46-47 0.6738762567659364 1103 48-49 0.7357954323110434 1103 50-51 0.6234410695381527 1103 52-53 0.6025566427507627 1103 54-55 0.5922879343604635 1103 56-57 0.5695462258122177 1103 58-59 0.532319006178291 1103 60-61 0.5963045966562674 1103 62-63 0.6072298241795195 1103 64-65 0.4622248820937642 1103 66-67 0.4845630676531556 1103 68-69 0.37180859831615365 1103 70-71 0.3885456626761261 1103 72-73 0.47279972104276524 1103 74-75 0.4260334881240304 1103 76-77 0.2057076357748251 1103 78-79 0.29021336754751204 1103 80-81 0.17689737064108257 1103 82-83 0.24426379210697036 1103 84-85 0.1955854310293148 1103 86-87 0.19430027405165973 1103 88-89 -0.008224837532559093 1103 90-91 -0.056831586451995264 1103 92-93 -0.06737554375751742 1103 94-95 0.019901176280544774 1103 96-97 -0.019098567819799683 1103 98-99 -0.24770081448698278 1103 100-101 -0.16520112596353442 1104 1 0.2868631201916294 1104 2 0.29444283505883817 1104 3 0.36171163795924244 1104 4 0.32232385283194276 1104 5 0.318176064158024 1104 6 0.24410203856579216 1104 7 0.35104495695257754 1104 8 0.31864057509100263 1104 9 0.3952862682243108 1104 10-11 0.37766288027058437 1104 12-13 0.5008105984236124 1104 14-15 0.5465434853035234 1104 16-17 0.5751310850605336 1104 18-19 0.4682802401245212 1104 20-21 0.538609682882857 1104 22-23 0.629165749185546 1104 24-25 0.5539502217841203 1104 26-27 0.4874386893118796 1104 28-29 0.5342921426717382 1104 30-31 0.48280399484414716 1104 32-33 0.4986469407512928 1104 34-35 0.456837719187952 1104 36-37 0.6833433686045822 1104 38-39 0.5989768158918807 1104 40-41 0.4492296793260806 1104 42-43 0.4135094269848736 1104 44-45 0.5885895313006486 1104 46-47 0.6162821278571968 1104 48-49 0.6016218522216903 1104 50-51 0.573504885747468 1104 52-53 0.6268068022912843 1104 54-55 0.6256319037286815 1104 56-57 0.7099418863099913 1104 58-59 0.6516553176013957 1104 60-61 0.6479957262031704 1104 62-63 0.6865214642561028 1104 64-65 0.4899849331467294 1104 66-67 0.4721189068363074 1104 68-69 0.5825616295860989 1104 70-71 0.5386732950947568 1104 72-73 0.5477837669904382 1104 74-75 0.45857975487578173 1104 76-77 0.47325709333704324 1104 78-79 0.5384584217912938 1104 80-81 0.5143255774056037 1104 82-83 0.47862882082426594 1104 84-85 0.48722550760876615 1104 86-87 0.5783143136177102 1104 88-89 0.5032620801445304 1104 90-91 0.4635896005294988 1104 92-93 0.3973660005947508 1104 94-95 0.4721985598159648 1104 96-97 0.47531751386494747 1104 98-99 0.45587289323477975 1104 100-101 0.708761860635061 1105 1 0.09704176498216555 1105 2 0.16227508124326206 1105 3 0.2671775855974765 1105 4 0.2525624247778211 1105 5 0.18439707284385065 1105 6 0.16593185064085247 1105 7 0.1303232816877724 1105 8 0.1989265220472305 1105 9 0.20459413840298168 1105 10-11 0.24826556996953997 1105 12-13 0.2576024374183987 1105 14-15 0.29881653180491696 1105 16-17 0.17246511420164978 1105 18-19 0.2925594457832119 1105 20-21 0.3338624592127175 1105 22-23 0.2866502092078562 1105 24-25 0.30087957415513955 1105 26-27 0.21502832023335117 1105 28-29 0.33198755342566244 1105 30-31 0.28504620902303657 1105 32-33 0.24452902934377363 1105 34-35 0.272486513256446 1105 36-37 0.20728114536476028 1105 38-39 0.379649800850423 1105 40-41 0.33324128315894797 1105 42-43 0.27058111142427066 1105 44-45 0.3789286218953052 1105 46-47 0.373456072494605 1105 48-49 0.362707482857374 1105 50-51 0.4092865368822842 1105 52-53 0.33727404086810964 1105 54-55 0.25017916182383715 1105 56-57 0.2771137240381947 1105 58-59 0.3292915116221522 1105 60-61 0.3831560218037495 1105 62-63 0.32553200448548836 1105 64-65 0.44223390648519967 1105 66-67 0.2012940985691074 1105 68-69 0.16127711727671468 1105 70-71 0.05809045256166101 1105 72-73 0.15549716563389637 1105 74-75 0.09328544117608217 1105 76-77 0.11265735087523154 1105 78-79 0.053449243136757474 1105 80-81 0.21682725806149605 1105 82-83 0.16486345743722808 1105 84-85 0.008047880142786568 1105 86-87 0.14011678052827392 1105 88-89 0.05744283346808743 1105 90-91 0.0932437791921501 1105 92-93 0.19800052938592572 1105 94-95 0.37543976966087556 1105 96-97 0.3742925370450081 1105 98-99 0.520262311760316 1105 100-101 0.61309062534486 1106 1 0.14435298599782698 1106 2 0.16881396758002154 1106 3 0.13930928860757774 1106 4 0.1918276715906444 1106 5 0.18200485695308544 1106 6 0.09229892423672226 1106 7 0.11415281451741066 1106 8 0.144946907603682 1106 9 0.20499691360147665 1106 10-11 0.12008450113692959 1106 12-13 0.14698424061323578 1106 14-15 0.24223924132132169 1106 16-17 0.29855676123862196 1106 18-19 0.3546330497945078 1106 20-21 0.3138098337211659 1106 22-23 0.20290738096846894 1106 24-25 0.25854500928267043 1106 26-27 0.11722684564443142 1106 28-29 0.19467806731631043 1106 30-31 0.1658227139931725 1106 32-33 0.30640837271838706 1106 34-35 0.3723627484608727 1106 36-37 0.4449437622876715 1106 38-39 0.4192598297492296 1106 40-41 0.42095423680029853 1106 42-43 0.48116839757467034 1106 44-45 0.47468873077900753 1106 46-47 0.5259956739711455 1106 48-49 0.4880324723501914 1106 50-51 0.45068237897159236 1106 52-53 0.33832760956225627 1106 54-55 0.38040892695173767 1106 56-57 0.44611721991514486 1106 58-59 0.36836844372133726 1106 60-61 0.5043196796704521 1106 62-63 0.26989241864753666 1106 64-65 0.26547444269342435 1106 66-67 0.2648440019682212 1106 68-69 0.46444975593076165 1106 70-71 0.391250906145995 1106 72-73 0.2794896167551535 1106 74-75 0.2866800500972495 1106 76-77 0.20172239112831747 1106 78-79 0.4138965964679393 1106 80-81 0.3439781753626683 1106 82-83 0.2731161135543658 1106 84-85 0.4777935168126781 1106 86-87 0.580456773295424 1106 88-89 0.6692850351863768 1106 90-91 0.7213896204669545 1106 92-93 0.8952229280853174 1106 94-95 0.8605499158491305 1106 96-97 0.9206192190896267 1106 98-99 0.9365257959734805 1106 100-101 1.0704105046018952 1107 1 0.16453762803676142 1107 2 0.16928067952540715 1107 3 0.11956867251583247 1107 4 0.26585288323001777 1107 5 0.14216984312361802 1107 6 0.20323635365528503 1107 7 0.0806273064609826 1107 8 0.1980571279756731 1107 9 0.17995726807069445 1107 10-11 0.1839871104464592 1107 12-13 0.23852194354299172 1107 14-15 0.350355711835725 1107 16-17 0.25626611302669744 1107 18-19 0.43304245550930176 1107 20-21 0.27629173988868416 1107 22-23 0.348586567774376 1107 24-25 0.2386947108868469 1107 26-27 0.3848593628548045 1107 28-29 0.2956830770243286 1107 30-31 0.25255788104806953 1107 32-33 0.36686975595265636 1107 34-35 0.34302525556149277 1107 36-37 0.3957837538448814 1107 38-39 0.47125513824482823 1107 40-41 0.5064710558196026 1107 42-43 0.6038373363056877 1107 44-45 0.5388081063004364 1107 46-47 0.7352108463667477 1107 48-49 0.7735660410316072 1107 50-51 0.8207614661589844 1107 52-53 0.7467543785604249 1107 54-55 0.7836957183440916 1107 56-57 0.780984339928672 1107 58-59 0.8682861445164036 1107 60-61 0.669560942762871 1107 62-63 0.6614478006657478 1107 64-65 0.5898470399264113 1107 66-67 0.5629821411087406 1107 68-69 0.5175982526785639 1107 70-71 0.6291909495512051 1107 72-73 0.45154317529881993 1107 74-75 0.40089985363450253 1107 76-77 0.35981451666843256 1107 78-79 0.4611261963588902 1107 80-81 0.5683656833927095 1107 82-83 0.6113480567989953 1107 84-85 0.4414285112940881 1107 86-87 0.5842206502305807 1107 88-89 0.576545124259404 1107 90-91 0.5359642150271924 1107 92-93 0.5846108378673982 1107 94-95 0.7648940046775046 1107 96-97 0.7335299905614718 1107 98-99 0.7431936205921303 1107 100-101 0.8307557695043997 1108 1 0.1449383622350986 1108 2 0.14146778607145905 1108 3 -0.054441779744369256 1108 4 0.08800845447895256 1108 5 0.13797303377218384 1108 6 0.1776594385937571 1108 7 0.14267418316897817 1108 8 0.11574380946366603 1108 9 0.11938846096212075 1108 10-11 0.2014457508426304 1108 12-13 0.3205791360679484 1108 14-15 0.06351928015289587 1108 16-17 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82-83 0.801894289825178 1108 84-85 0.8771043693333489 1108 86-87 0.8422277944302508 1108 88-89 0.8504743003860007 1108 90-91 0.7769578305057543 1108 92-93 0.8514021548081452 1108 94-95 0.8735586126371366 1108 96-97 0.9183859603662263 1108 98-99 1.1321478912656389 1108 100-101 1.2825277523982663 1109 1 0.13995337771417837 1109 2 0.29877424607568415 1109 3 0.19514763404239943 1109 4 0.32661785284673783 1109 5 0.24592758898261735 1109 6 0.24762672047439338 1109 7 0.25219660679951517 1109 8 0.20569567236178443 1109 9 0.253746092070692 1109 10-11 0.2927967833821796 1109 12-13 0.3457080640022241 1109 14-15 0.31412045735272187 1109 16-17 0.296718081518236 1109 18-19 0.4777633853805625 1109 20-21 0.4721493639932106 1109 22-23 0.4551293326201318 1109 24-25 0.45939750293953807 1109 26-27 0.4317686169389887 1109 28-29 0.3684477567798652 1109 30-31 0.44753118381190404 1109 32-33 0.5246981572831189 1109 34-35 0.5642009874130807 1109 36-37 0.5256380507176388 1109 38-39 0.553572148477862 1109 40-41 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70-71 -2.225489289000283 2316 72-73 -2.3313931264487486 2316 74-75 -2.370420519114603 2316 76-77 -2.4281198184447526 2316 78-79 -2.3107735818174895 2316 80-81 -2.2616302728060944 2316 82-83 -2.16917142505865 2316 84-85 -2.268290774715066 2316 86-87 -2.332004191110876 2316 88-89 -2.4891767616975358 2316 90-91 -2.8380781220819493 2316 92-93 -3.0047403076748758 2316 94-95 -3.061171305568788 2316 96-97 -3.0985013549396108 2316 98-99 -3.206889601904333 2316 100-101 -3.456713158468638 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12948.0 3 4257.0 4 4196.0 5 4636.0 6 5221.0 7 6313.0 8 7697.0 9 9114.0 10 10945.0 11 13973.0 12 16921.0 13 21324.0 14 23037.0 15 23543.0 16 28125.0 17 35883.0 18 30734.0 19 22496.0 20 21974.0 21 23109.0 22 24940.0 23 27954.0 24 31775.0 25 36026.0 26 41292.0 27 48043.0 28 55929.0 29 65801.0 30 78373.0 31 94213.0 32 115569.0 33 145143.0 34 192149.0 35 272224.0 36 389143.0 37 565589.0 38 514988.0 39 89069.0 40 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.645239714628886 18.78792848678382 14.346635537611327 22.220196260975968 2 15.444423413055864 20.389163528829908 40.1795002235076 23.986912834606624 3 19.428316930183893 25.32573865586291 29.75291805537525 25.493026358577946 4 12.684726610080702 16.820558639108594 37.26034793619777 33.23436681461294 5 13.417637857441816 37.733421713997686 33.93447829583906 14.91446213272144 6 31.041016069763582 36.25632610638811 17.71235916514185 14.990298658706461 7 28.07875808365308 33.379456809347516 20.70025457846162 17.841530528537778 8 26.36002603037434 34.47151980254946 19.791532162480415 19.376922004595787 9 26.848768579711074 16.970200191117073 20.51771847973597 35.66331274943588 10-11 24.917242952080247 26.79595481365612 26.97304815101515 21.313754083248483 12-13 26.293339166575525 24.40037819710095 27.545880168309594 21.760402468013933 14-15 23.024473601161485 25.549485626366636 26.54034647045172 24.885694302020152 16-17 21.729087948473875 28.964879486987176 27.135338465410197 22.170694099128752 18-19 21.5843818358779 27.159406649813533 30.07670637967088 21.179505134637687 20-21 22.55266564121722 26.137573513787594 30.07617953320903 21.233581311786153 22-23 22.1226545116456 26.206918929616236 29.98365073419094 21.686775824547222 24-25 21.981809684332426 26.41710290038994 30.20610667768156 21.39498073759608 26-27 21.71122815947025 26.53950510651315 30.21528200857041 21.533984725446192 28-29 21.40081315192678 26.758173166436222 30.290334981388494 21.5506787002485 30-31 21.546050544485233 26.479306027285705 30.23355962994761 21.74108379828145 32-33 21.367142905784576 26.760199181405795 30.06581453721032 21.806843375599314 34-35 22.047278957894793 26.62825718253811 29.814347719823292 21.5101161397438 36-37 22.026515628034144 26.38913350197121 29.90056236278819 21.683788507206465 38-39 21.722086649507858 26.69672736748175 29.999835528278336 21.58135045473206 40-41 21.83951182701991 26.592582454473977 30.02054903500784 21.547356683498272 42-43 21.687188980517238 26.761108011442612 29.58264128306925 21.969061724970892 44-45 21.776177691308312 26.883248161197887 29.4110930631908 21.929481084302996 46-47 22.015404064390726 27.099932251016035 28.889885887875348 21.99477779671789 48-49 22.11368976725641 27.189285949316943 28.67325700379239 22.023767279634257 50-51 21.943557185907117 27.409109199596482 28.685616412396875 21.96171720209953 52-53 22.175964299464244 27.433012705283645 28.494203001635967 21.89681999361615 54-55 21.856071828130887 27.27701719468456 28.644195969440915 22.22271500774363 56-57 21.97288658337671 27.629512533803897 28.35913812885887 22.03846275396053 58-59 22.11043279065906 27.179130872142416 28.477277160447056 22.233159176751467 60-61 21.97203840336034 27.194419026211097 28.93505678087735 21.89848578955121 62-63 21.85714076085791 27.204908789835663 28.91892811468033 22.019022334626097 64-65 21.698987490383765 27.29614103608704 28.775279066439907 22.229592407089292 66-67 21.662780863101684 27.4742416633745 28.428547484852945 22.434429988670864 68-69 21.604450486211295 27.32148670917056 28.69479121325182 22.379271591366326 70-71 21.70639919865427 27.281892138722895 28.478232005979443 22.53347665664339 72-73 21.816515031240378 27.13724327488644 28.525134497431843 22.521107196441342 74-75 21.64898699720046 27.580816660720036 28.3232692236968 22.446927118382707 76-77 21.811734239112294 27.308529237545553 28.345367040291887 22.53436948305027 78-79 21.80015326367301 27.304263538707623 28.367983724965068 22.5275994726543 80-81 21.75383453487037 27.54580094084929 28.20408264614186 22.496281878138475 82-83 21.811124959423942 27.414805596940134 28.24906386820735 22.525005575428576 84-85 21.737481453961077 27.342178671180445 28.17725635209392 22.74308352276456 86-87 21.854057712680305 27.273711013428702 28.23010049798245 22.642130775908544 88-89 21.912391337552332 27.18276903940598 28.05011702326869 22.854722599773 90-91 21.881817815234488 27.19462881567805 28.18600750030243 22.73754586878503 92-93 22.000189689902175 27.089653641723523 28.18527412771679 22.724882540657514 94-95 21.784857683791262 26.889361735762048 28.235152546348164 23.09062803409853 96-97 21.867068607242995 26.72753739237825 28.251413314303097 23.153980686075656 98-99 21.84148065587203 26.754310904505633 27.98003446949321 23.42417397012913 100-101 22.12782565431643 26.232184054491896 28.41439193749592 23.22559835369576 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5037.0 1 3017.5 2 1415.5 3 1875.5 4 1531.0 5 1393.0 6 2478.5 7 4932.0 8 7619.5 9 8410.5 10 6896.5 11 5326.0 12 5126.0 13 5378.0 14 5738.5 15 6160.0 16 6514.0 17 6851.0 18 7070.0 19 7380.5 20 7920.0 21 8759.0 22 9975.0 23 11738.5 24 13595.5 25 15907.0 26 19617.0 27 24057.5 28 27736.0 29 31651.0 30 37676.0 31 44526.0 32 51760.5 33 57412.5 34 63422.5 35 69029.5 36 74927.0 37 81332.0 38 84288.0 39 85507.5 40 86353.0 41 89584.5 42 92759.5 43 97154.0 44 102575.0 45 106331.0 46 107863.5 47 111145.0 48 124686.0 49 125464.0 50 114517.5 51 108962.5 52 103068.5 53 101016.0 54 98845.5 55 92327.0 56 84217.0 57 75674.5 58 67992.0 59 60145.5 60 50572.5 61 40984.0 62 33963.0 63 27405.0 64 21025.5 65 16775.0 66 13135.5 67 10719.0 68 8083.0 69 5455.0 70 4072.5 71 3149.0 72 2510.0 73 2010.0 74 1576.0 75 1261.0 76 917.5 77 574.5 78 385.5 79 275.5 80 186.5 81 138.5 82 112.5 83 79.0 84 51.0 85 34.0 86 23.5 87 19.0 88 16.5 89 14.0 90 10.5 91 6.5 92 5.0 93 5.0 94 5.0 95 4.0 96 3.0 97 2.0 98 2.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1633211567517101 2 0.09538571981704948 3 0.08726300453138354 4 0.10376527985483135 5 0.12636761804103225 6 0.039650408607895024 7 0.04302149597941646 8 0.04610363300480749 9 0.04645679453896688 10-11 0.04469098686816994 12-13 0.049137611639176786 14-15 0.047179170404292904 16-17 0.04583073545568433 18-19 0.04982788191048832 20-21 0.05186658713040843 22-23 0.06562383416561735 24-25 0.08255953500826077 26-27 0.08405244512993455 28-29 0.07273522323982685 30-31 0.06162668771081336 32-33 0.05072683854289403 34-35 0.29662358309987213 36-37 0.46835640548337865 38-39 0.44578617289119216 40-41 0.4211130238910567 42-43 0.4505056952113222 44-45 0.4602015846036982 46-47 0.4593186807682997 48-49 0.47612595923488515 50-51 0.4656755883831687 52-53 0.47235355193818257 54-55 0.46336398561412545 56-57 0.4413877065112071 58-59 0.42080802074791906 60-61 0.39107824069050146 62-63 0.4494301578118368 64-65 0.4862873797404712 66-67 0.4877321314711232 68-69 0.4994025148953903 70-71 0.4955658964106588 72-73 0.4856773734541959 74-75 0.47927230744830523 76-77 0.4601855318066909 78-79 0.43440473981305555 80-81 0.4396540044344246 82-83 0.4515170214227786 84-85 0.4492375242477499 86-87 0.4594792087383721 88-89 0.47187196802796527 90-91 0.4766717543331315 92-93 0.4792080962602762 94-95 0.4834299818731816 96-97 0.48456973046069596 98-99 0.4777151861386024 100-101 0.46374925274229933 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3114722.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.008024261832716 #Duplication Level Percentage of deduplicated Percentage of total 1 73.94896584909839 36.980416783191515 2 13.99176372625719 13.99400919777001 3 5.045306347081769 7.569174066397312 4 2.291686370280416 4.584108304219777 5 1.2757419940420744 3.189866829494745 6 0.7533059045157025 2.2602803969761855 7 0.5048140455536752 1.7671327126433483 8 0.3593308561383753 1.4375540937434397 9 0.2602509252017379 1.171317112348763 >10 1.3712786518446873 12.766535464809095 >50 0.12481729889610334 4.307523372425669 >100 0.06759453845886891 6.093973173482429 >500 0.0037290321576332157 1.3076856573672604 >1k 0.0012215794997047866 1.2388482508243763 >5k 6.42936578791993E-5 0.29592689783778153 >10k+ 1.285873157583986E-4 1.035647686468142 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17755 0.570034821727268 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14456 0.46411846707346593 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9204 0.29549988730936505 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4415 0.1417461975739729 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4126 0.1324676809037853 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3649 0.11715331255887362 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.42111880289798E-5 0.0 5 0.0 0.0 0.0 6.42111880289798E-5 0.0 6 0.0 0.0 0.0 6.42111880289798E-5 0.0 7 3.21055940144899E-5 0.0 0.0 6.42111880289798E-5 0.0 8 3.21055940144899E-5 0.0 0.0 1.284223760579596E-4 0.0 9 3.21055940144899E-5 0.0 0.0 2.568447521159192E-4 0.0 10-11 3.21055940144899E-5 0.0 0.0 2.568447521159192E-4 0.0 12-13 3.21055940144899E-5 0.0 0.0 3.0500314313765405E-4 0.0 14-15 3.21055940144899E-5 0.0 0.0 3.8526712817387875E-4 0.0 16-17 3.21055940144899E-5 0.0 0.0 4.334255191956136E-4 0.0 18-19 3.21055940144899E-5 0.0 0.0 5.136895042318384E-4 0.0 20-21 3.21055940144899E-5 0.0 0.0 5.939534892680631E-4 0.0 22-23 3.21055940144899E-5 0.0 0.0 8.026398503622474E-4 0.0 24-25 3.21055940144899E-5 0.0 0.0 0.0013163293545940856 0.0 26-27 3.21055940144899E-5 0.0 0.0 0.0019584412348838836 0.0 28-29 3.21055940144899E-5 0.0 0.0 0.004173727221883686 0.0 30-31 3.21055940144899E-5 0.0 0.0 0.011525908251201873 0.0 32-33 3.21055940144899E-5 0.0 0.0 0.024721307391157223 0.0 34-35 3.21055940144899E-5 0.0 0.0 0.04677785047911178 0.0 36-37 3.21055940144899E-5 0.0 0.0 0.08679747341817343 0.0 38-39 3.21055940144899E-5 0.0 0.0 0.14384911398192196 0.0 40-41 3.21055940144899E-5 0.0 0.0 0.20550790728674984 0.0 42-43 3.21055940144899E-5 0.0 0.0 0.26785697086288923 0.0 44-45 3.21055940144899E-5 0.0 0.0 0.34840990624524437 0.0 46-47 3.21055940144899E-5 0.0 0.0 0.452127027709054 0.0 48-49 3.21055940144899E-5 0.0 0.0 0.564849768293928 0.0 50-51 3.21055940144899E-5 0.0 0.0 0.6852939042392868 0.0 52-53 3.21055940144899E-5 0.0 0.0 0.8008740426914505 0.0 54-55 3.21055940144899E-5 0.0 0.0 0.9322661861957504 0.0 56-57 3.21055940144899E-5 0.0 0.0 1.0949131254731563 0.0 58-59 3.21055940144899E-5 0.0 0.0 1.2771765826934152 0.0 60-61 3.21055940144899E-5 0.0 0.0 1.4778204924869698 0.0 62-63 3.21055940144899E-5 0.0 0.0 1.6732151376591555 0.0 64-65 3.21055940144899E-5 0.0 0.0 1.8806493805867748 0.0 66-67 3.21055940144899E-5 0.0 0.0 2.122837929035079 0.0 68-69 3.21055940144899E-5 0.0 0.0 2.396313378850504 0.0 70-71 3.21055940144899E-5 0.0 0.0 2.6950559311553324 0.0 72-73 3.21055940144899E-5 0.0 0.0 3.0029485777542906 0.0 74-75 3.21055940144899E-5 0.0 0.0 3.3134257246714154 0.0 76-77 3.21055940144899E-5 0.0 0.0 3.6512568376888854 0.0 78-79 3.21055940144899E-5 0.0 0.0 4.03946162771509 0.0 80-81 3.21055940144899E-5 0.0 0.0 4.4646039036549645 0.0 82-83 3.21055940144899E-5 0.0 0.0 4.895059655404238 0.0 84-85 3.21055940144899E-5 1.605279700724495E-5 0.0 5.329159392074156 0.0 86-87 3.21055940144899E-5 3.21055940144899E-5 0.0 5.775154251326443 0.0 88-89 3.21055940144899E-5 3.21055940144899E-5 0.0 6.263287702722748 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 22885 0.0 28.671482 1 GGTATCA 15965 0.0 27.528069 1 GTACATG 15645 0.0 24.394125 1 TATCAAC 27430 0.0 23.668526 2 ATCAACG 27425 0.0 23.655561 3 TCAACGC 27605 0.0 23.535646 4 TACATGG 15890 0.0 23.351477 2 CAACGCA 27830 0.0 23.311312 5 AACGCAG 28145 0.0 23.048353 6 TACCTGG 2245 0.0 23.00841 2 GAGTACT 14660 0.0 22.885551 12-13 ACATGGG 15780 0.0 22.73345 3 GTACTTT 15865 0.0 21.013674 14-15 ACGCAGA 30690 0.0 20.98377 7 CGCAGAG 30875 0.0 20.79669 8 GTACGCT 115 0.003775568 20.60386 3 AGTACTT 15470 0.0 20.46287 12-13 GCAGAGT 31815 0.0 19.691072 9 GTATAGG 995 0.0 19.535555 1 CATGGGG 6910 0.0 18.9967 4 >>END_MODULE