FastQCFastQC Report
Thu 26 May 2016
SRR938681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114722
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173390.5566788946172403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154230.4951645764854777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100220.32176226321321777No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48000.1541068512695515No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44610.14322305489863943No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37030.11888701463565608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171250.028.4556061
GTATCAA241750.027.112821
TACCTGG23100.023.0065982
GTACATG158100.022.717591
ATCAACG286400.022.6817593
TATCAAC288400.022.5573732
TCAACGC287850.022.551024
GAGTACT156950.022.4791512-13
CAACGCA292350.022.2039015
AACGCAG296900.021.9115736
TACATGG164850.021.3580322
GTACTTT165750.021.199814-15
ACATGGG161850.020.6398373
AGTACTT163250.020.39082112-13
ACGCAGA327500.019.7918197
CGCAGAG329400.019.6344418
TATAACG2251.6860413E-618.9804422
GCAGAGT339400.018.8462269
ACTTTTT188400.018.78932616-17
AGAGTAC274150.018.40812310-11
Produced by FastQC (version 0.11.5)