FastQCFastQC Report
Thu 26 May 2016
SRR938680_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938680_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122698
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181180.5802034010333372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143110.4582895944468533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91260.2922472810371032No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42340.13558787945552211No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40940.13110457687550955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36610.11723836246732794No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159300.029.238941
GTATCAA225800.027.9738561
GTACATG162550.025.3503171
TACATGG165850.024.5128062
TATCAAC266750.023.4745452
ATCAACG267000.023.4710873
TCAACGC268150.023.3704264
GAGTACT140900.023.32816712-13
ACATGGG166600.023.1218553
CAACGCA272700.023.0152845
AACGCAG277400.022.6926486
GTACTTT152300.021.48936714-15
GTGTAGC14100.020.561841
ACGCAGA305050.020.4339357
CGCAGAG307250.020.2104248
AGTACTT148750.019.91129312-13
TACCTGG22950.019.6367032
CATGGGG71050.019.6302074
GCAGAGT314700.019.1283999
ACTTTTT178550.018.74271416-17
Produced by FastQC (version 0.11.5)