##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938680_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3122698 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4539721740623 33.0 31.0 34.0 30.0 34.0 2 31.52087841987922 33.0 31.0 34.0 28.0 34.0 3 31.524287010783624 33.0 31.0 34.0 28.0 34.0 4 35.07987323782191 37.0 35.0 37.0 32.0 37.0 5 35.113286331243046 37.0 35.0 37.0 32.0 37.0 6 35.17003917765983 37.0 35.0 37.0 32.0 37.0 7 35.0799638645812 37.0 35.0 37.0 32.0 37.0 8 35.043627337642 37.0 35.0 37.0 32.0 37.0 9 36.59585621151965 39.0 37.0 39.0 32.0 39.0 10-11 36.46881510796113 39.0 37.0 39.0 32.0 39.0 12-13 36.34749774073573 39.0 37.0 39.0 32.0 39.0 14-15 37.471657842032755 40.0 37.0 41.0 32.0 41.0 16-17 37.35438601491403 40.0 37.0 41.0 32.0 41.0 18-19 37.317785613594395 40.0 37.0 41.0 31.5 41.0 20-21 37.22141013956521 40.0 37.0 41.0 31.0 41.0 22-23 37.23613122370463 40.0 37.0 41.0 31.0 41.0 24-25 37.16190678701558 40.0 37.0 41.0 31.0 41.0 26-27 37.01909054285749 40.0 37.0 41.0 30.5 41.0 28-29 36.83931123022463 40.0 36.0 41.0 30.0 41.0 30-31 36.59086053150193 39.0 36.0 41.0 30.0 41.0 32-33 36.37379311095725 39.0 35.0 41.0 30.0 41.0 34-35 36.066943873534996 39.0 35.0 41.0 29.0 41.0 36-37 35.842118898465365 39.0 35.0 41.0 27.5 41.0 38-39 35.65986416233655 39.0 35.0 40.5 27.0 41.0 40-41 35.52594551250233 39.0 35.0 40.5 26.5 41.0 42-43 35.47716173642152 39.0 35.0 41.0 26.0 41.0 44-45 35.25774506532492 39.0 35.0 41.0 25.0 41.0 46-47 34.90004973263505 39.0 34.5 41.0 24.0 41.0 48-49 34.56827173168843 39.0 34.0 41.0 23.0 41.0 50-51 33.42616817252261 38.0 32.5 39.5 20.5 40.5 52-53 33.493259034335054 37.5 33.0 39.5 20.0 40.5 54-55 33.973758109173545 38.0 34.0 40.0 20.0 41.0 56-57 33.861729504422144 38.0 34.0 40.0 19.0 41.0 58-59 33.6491857361807 38.0 33.0 40.0 18.0 41.0 60-61 33.33586773360729 37.5 33.0 40.0 16.0 41.0 62-63 32.94127514091981 37.0 32.5 40.0 13.0 41.0 64-65 32.54645982416487 36.0 32.0 39.5 9.0 41.0 66-67 32.15514660719673 36.0 31.5 39.0 7.5 41.0 68-69 31.738529630466985 35.0 31.0 39.0 6.5 40.5 70-71 31.31211695783582 35.0 31.0 38.5 2.0 40.0 72-73 30.884411813118014 35.0 30.5 37.5 2.0 40.0 74-75 30.41632924477487 35.0 30.0 37.0 2.0 39.0 76-77 29.95859750126333 35.0 29.5 36.5 2.0 39.0 78-79 29.520597733114123 34.0 29.0 36.0 2.0 38.5 80-81 29.04693121140757 34.0 29.0 35.5 2.0 37.0 82-83 28.628811047369936 34.0 29.0 35.0 2.0 37.0 84-85 28.27680694706949 34.0 28.0 35.0 2.0 36.5 86-87 27.848291925764194 34.0 27.0 35.0 2.0 36.0 88-89 27.388812334718246 34.0 26.0 35.0 2.0 36.0 90-91 26.935492641299284 33.5 25.5 35.0 2.0 35.0 92-93 26.462871849919523 33.0 25.0 35.0 2.0 35.0 94-95 25.94059944317382 33.0 23.5 35.0 2.0 35.0 96-97 25.327126094166005 33.0 19.0 35.0 2.0 35.0 98-99 24.552311975093332 32.0 6.0 35.0 2.0 35.0 100-101 22.069441553425918 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.028693754187226972 1101 2 0.16777995263247547 1101 3 0.2510755407947478 1101 4 0.13679139363063797 1101 5 0.04218568644353837 1101 6 0.23356757804449302 1101 7 0.2772566482528589 1101 8 0.26343033959656026 1101 9 0.3585219839237155 1101 10-11 0.2101957533358103 1101 12-13 0.21988092398518688 1101 14-15 0.33493951200479444 1101 16-17 0.5589527151658089 1101 18-19 0.4330282589271306 1101 20-21 0.5091471459455548 1101 22-23 0.6011336719076112 1101 24-25 0.5860927633359125 1101 26-27 0.523757119238276 1101 28-29 0.430717004507315 1101 30-31 0.5355516209741893 1101 32-33 0.3824864188012995 1101 34-35 0.39482621902526915 1101 36-37 0.564335395143921 1101 38-39 0.6102690784212044 1101 40-41 0.637310590247246 1101 42-43 0.6775700649019285 1101 44-45 0.7634270162194099 1101 46-47 0.6677236571346086 1101 48-49 0.655854596191368 1101 50-51 0.7527821309429044 1101 52-53 0.7952101248771655 1101 54-55 0.7952359281016612 1101 56-57 0.6383279719436032 1101 58-59 0.7675637246380234 1101 60-61 0.5992545720101177 1101 62-63 0.4940627450659534 1101 64-65 0.4461707190420654 1101 66-67 0.3353701058534071 1101 68-69 0.4822307668203045 1101 70-71 0.5515934147961197 1101 72-73 0.43610845682145793 1101 74-75 0.39003140869084163 1101 76-77 0.4185999685723729 1101 78-79 0.39837007859378204 1101 80-81 0.5162386901801312 1101 82-83 0.35578703288862457 1101 84-85 0.38552591237600353 1101 86-87 0.29608300271330634 1101 88-89 0.3573733757216715 1101 90-91 0.16379745407255797 1101 92-93 0.17950597302611015 1101 94-95 0.11793137504347584 1101 96-97 0.18698423521133023 1101 98-99 0.10138557777485246 1101 100-101 0.41982414042225 1102 1 0.19081310035829802 1102 2 0.22918837179951623 1102 3 0.22180438092014043 1102 4 0.3626646582970068 1102 5 0.30572128877224003 1102 6 0.43990994257651295 1102 7 0.3702425416697892 1102 8 0.41115761232383363 1102 9 0.5217832915815777 1102 10-11 0.5500580462153479 1102 12-13 0.5037507179842962 1102 14-15 0.6510333319779207 1102 16-17 0.7399843989992192 1102 18-19 0.6693667049638492 1102 20-21 0.6088140738452452 1102 22-23 0.6466246033899168 1102 24-25 0.6004512496771568 1102 26-27 0.6163427663497885 1102 28-29 0.7271287823846038 1102 30-31 0.7822320956719011 1102 32-33 0.6318286713763115 1102 34-35 0.6584429901851436 1102 36-37 0.7226565998013328 1102 38-39 0.6066752584480781 1102 40-41 0.7836328025177366 1102 42-43 0.705965161184956 1102 44-45 0.7256023408947385 1102 46-47 0.8690671412367124 1102 48-49 0.907858626643673 1102 50-51 0.9060792648434841 1102 52-53 0.778257034863735 1102 54-55 0.7476318080837459 1102 56-57 0.7994117157859719 1102 58-59 0.7797446471637741 1102 60-61 0.5913644018354702 1102 62-63 0.41342570968753733 1102 64-65 0.5652146061894001 1102 66-67 0.4588642840889534 1102 68-69 0.4914056436675942 1102 70-71 0.3638051254992085 1102 72-73 0.39232207079369275 1102 74-75 0.17874054438273745 1102 76-77 0.36256268688853766 1102 78-79 0.3757715564262938 1102 80-81 0.38036206680351015 1102 82-83 0.3940847265742491 1102 84-85 0.20980938752155254 1102 86-87 0.1828748746344857 1102 88-89 0.22479388624563512 1102 90-91 0.15623195922258049 1102 92-93 0.1786243115841053 1102 94-95 0.10993484571073608 1102 96-97 0.02899610265422936 1102 98-99 -0.16968193230128037 1102 100-101 0.15081943822789157 1103 1 0.16806551661673907 1103 2 0.227197269839575 1103 3 0.2719792182002969 1103 4 0.3246724088901374 1103 5 0.28990489702107425 1103 6 0.24218773506515134 1103 7 0.17258786460609343 1103 8 0.2963176157326899 1103 9 0.33196385848732035 1103 10-11 0.16079133556828396 1103 12-13 0.3402239793510944 1103 14-15 0.4444045242118122 1103 16-17 0.44750199305867255 1103 18-19 0.44481003759715065 1103 20-21 0.44551465601477247 1103 22-23 0.3562414432388934 1103 24-25 0.43862442361580634 1103 26-27 0.43059537169347806 1103 28-29 0.5388473314577453 1103 30-31 0.6176905456141526 1103 32-33 0.5448046088511092 1103 34-35 0.6207094965288249 1103 36-37 0.5710129372389972 1103 38-39 0.6330598799199478 1103 40-41 0.5958794206690143 1103 42-43 0.558745598964876 1103 44-45 0.6198917950356702 1103 46-47 0.5350725801960365 1103 48-49 0.5634394569752317 1103 50-51 0.6799668766777103 1103 52-53 0.5579694331495588 1103 54-55 0.578003313050182 1103 56-57 0.6872099720927451 1103 58-59 0.7288171782886508 1103 60-61 0.6788382551874861 1103 62-63 0.516549363834244 1103 64-65 0.532363040776552 1103 66-67 0.5191723028255169 1103 68-69 0.3919257802959599 1103 70-71 0.4062161013465122 1103 72-73 0.5916896433955792 1103 74-75 0.5007457877891 1103 76-77 0.5574475217946642 1103 78-79 0.5585822801852949 1103 80-81 0.5864512016680905 1103 82-83 0.5087669777464221 1103 84-85 0.4373955563727776 1103 86-87 0.5026445273263427 1103 88-89 0.5837400916812427 1103 90-91 0.6385190556948643 1103 92-93 0.495762559536125 1103 94-95 0.5310255651630058 1103 96-97 0.5691210777345432 1103 98-99 0.6482702953284516 1103 100-101 0.9943147723495258 1104 1 0.07024999781666708 1104 2 0.16804342937151873 1104 3 0.09168749917696317 1104 4 0.3060771216534093 1104 5 0.20564800157710295 1104 6 0.2283740091847548 1104 7 0.2789346296748647 1104 8 0.2833228333942799 1104 9 0.28752077231535367 1104 10-11 0.23717952163013223 1104 12-13 0.38579289967609753 1104 14-15 0.571802696419276 1104 16-17 0.6027614925952776 1104 18-19 0.5719372344449454 1104 20-21 0.5102698068683011 1104 22-23 0.4682683142908175 1104 24-25 0.4357413007515305 1104 26-27 0.4918355660717495 1104 28-29 0.4867524488598747 1104 30-31 0.48429433690010626 1104 32-33 0.5229849090988168 1104 34-35 0.6108612787402521 1104 36-37 0.6951314026058881 1104 38-39 0.7101363784577472 1104 40-41 0.7144357128504808 1104 42-43 0.5944090171491752 1104 44-45 0.6577055322876504 1104 46-47 0.6182496490187575 1104 48-49 0.6536789899063891 1104 50-51 0.6623846080088782 1104 52-53 0.7197475981929315 1104 54-55 0.5851540964575292 1104 56-57 0.6564631528194269 1104 58-59 0.6594637381003352 1104 60-61 0.638566455388009 1104 62-63 0.7116762612303447 1104 64-65 0.7865918010275621 1104 66-67 0.7231669979817923 1104 68-69 0.7850391019165563 1104 70-71 0.746476292654453 1104 72-73 0.5862921212636358 1104 74-75 0.5548288970074751 1104 76-77 0.6115836984029386 1104 78-79 0.38890318627306897 1104 80-81 0.4073328879223084 1104 82-83 0.3622465401678916 1104 84-85 0.5074430422104186 1104 86-87 0.47592751297350944 1104 88-89 0.5987089335423192 1104 90-91 0.5234807050293426 1104 92-93 0.46981454852085136 1104 94-95 0.5074432699604898 1104 96-97 0.5005489600993016 1104 98-99 0.5876511315568003 1104 100-101 0.8208770857844812 1105 1 0.11752282680206605 1105 2 0.24783159578023017 1105 3 0.235441578278909 1105 4 0.3063838701196673 1105 5 0.299540070870556 1105 6 0.24944641674064627 1105 7 0.19917163715243902 1105 8 0.31872757354383197 1105 9 0.3807695089592116 1105 10-11 0.3553182390672518 1105 12-13 0.33098515357804814 1105 14-15 0.5398565685359316 1105 16-17 0.5134757783095623 1105 18-19 0.4601204257478386 1105 20-21 0.5342274215027203 1105 22-23 0.4185713332924408 1105 24-25 0.3990559556828117 1105 26-27 0.486212931950881 1105 28-29 0.5262339600389367 1105 30-31 0.6535938502049703 1105 32-33 0.6310209730218403 1105 34-35 0.5181976018610683 1105 36-37 0.6686102472177495 1105 38-39 0.7223261336183953 1105 40-41 0.7605966951914596 1105 42-43 0.6256139139362489 1105 44-45 0.7545681201631567 1105 46-47 0.7030101241525486 1105 48-49 0.5940471813025141 1105 50-51 0.6668525066420301 1105 52-53 0.7037607818504199 1105 54-55 0.6924279510457012 1105 56-57 0.6769822010600492 1105 58-59 0.6856245339680882 1105 60-61 0.7150195480465484 1105 62-63 0.7572064978648783 1105 64-65 0.7830455463424286 1105 66-67 0.7194855502036219 1105 68-69 0.7446298864094381 1105 70-71 0.6850142492807798 1105 72-73 0.7402546774386991 1105 74-75 0.6332749223305392 1105 76-77 0.6538483225661409 1105 78-79 0.6574833790863934 1105 80-81 0.800266950987556 1105 82-83 0.7446094357242323 1105 84-85 0.7455996610777795 1105 86-87 0.9174434471111645 1105 88-89 0.9451953116727339 1105 90-91 0.8673438709718724 1105 92-93 1.0147886287416341 1105 94-95 0.94103549029726 1105 96-97 0.9521475018786312 1105 98-99 0.9416920064892054 1105 100-101 1.2220527995107773 1106 1 0.09437801964314474 1106 2 0.16381194050466164 1106 3 0.1976051728085224 1106 4 0.1433219017114098 1106 5 0.20662974575708404 1106 6 0.14771891671294668 1106 7 0.19838128973196945 1106 8 0.12134846161364266 1106 9 0.21764660254890345 1106 10-11 0.21886891178585444 1106 12-13 0.22742757455253582 1106 14-15 0.2199670509802516 1106 16-17 0.31275541726113687 1106 18-19 0.30562421683020347 1106 20-21 0.31671227009948666 1106 22-23 0.1667889890629013 1106 24-25 0.017016785473742857 1106 26-27 0.13899078154111777 1106 28-29 -0.01562084501485117 1106 30-31 -0.15352610902196773 1106 32-33 -0.05717875220316415 1106 34-35 0.02723803145551784 1106 36-37 0.29479576834845744 1106 38-39 0.25776028918388505 1106 40-41 0.40226305409044727 1106 42-43 0.4154727224321064 1106 44-45 0.39988549635745585 1106 46-47 0.3511636103602527 1106 48-49 0.43141251755102417 1106 50-51 0.4100899580301274 1106 52-53 0.10352552423039896 1106 54-55 0.050897225366426824 1106 56-57 0.2662368000325941 1106 58-59 0.4136780818524315 1106 60-61 0.4824301815432577 1106 62-63 0.38069634337780656 1106 64-65 0.3552699126795389 1106 66-67 0.398302854380276 1106 68-69 0.371741388180336 1106 70-71 0.41434057043513306 1106 72-73 0.46874633262563137 1106 74-75 0.400999386537773 1106 76-77 0.512815164906602 1106 78-79 0.4346921969999471 1106 80-81 0.3808352994199744 1106 82-83 0.37983630975245575 1106 84-85 0.5206278218211509 1106 86-87 0.4875901045694242 1106 88-89 0.7399410709417751 1106 90-91 0.7526313136809186 1106 92-93 0.8232761844629763 1106 94-95 0.7872487474519332 1106 96-97 0.8423198640941116 1106 98-99 0.822467082477047 1106 100-101 1.2199027960022235 1107 1 0.055563395156742246 1107 2 -0.003122027243875891 1107 3 0.18432441064847538 1107 4 0.17969478421321838 1107 5 0.1800590276418106 1107 6 0.11016969276212762 1107 7 0.1272417457516397 1107 8 0.21305173939664712 1107 9 0.23249857501728854 1107 10-11 0.21470871947023795 1107 12-13 0.15180744842481175 1107 14-15 0.3483936434477286 1107 16-17 0.3384004409291208 1107 18-19 0.33744064515779115 1107 20-21 0.3443218406359492 1107 22-23 0.4483759904109519 1107 24-25 0.27589576281168604 1107 26-27 0.3423809331477301 1107 28-29 0.28333184241505194 1107 30-31 0.2682742552380546 1107 32-33 0.412741610957319 1107 34-35 0.46967103654869646 1107 36-37 0.2852180161440927 1107 38-39 0.19468388930319946 1107 40-41 0.3011122231013772 1107 42-43 0.31888986107787787 1107 44-45 0.5519066640028711 1107 46-47 0.6338104519851484 1107 48-49 0.6150610835232797 1107 50-51 0.5398246696774223 1107 52-53 0.548000294784849 1107 54-55 0.4985979036270578 1107 56-57 0.6087008411494779 1107 58-59 0.7150049508024026 1107 60-61 0.7317575021964515 1107 62-63 0.5922656759231373 1107 64-65 0.5870825891957594 1107 66-67 0.5626275951988333 1107 68-69 0.7336133451734348 1107 70-71 0.7567197314632459 1107 72-73 0.5977606314845225 1107 74-75 0.6141477766206584 1107 76-77 0.5843499956460505 1107 78-79 0.5955975425139144 1107 80-81 0.453830225559436 1107 82-83 0.249169124394772 1107 84-85 0.4489073042792029 1107 86-87 0.36913511163286117 1107 88-89 0.49613420638996786 1107 90-91 0.7718136101089321 1107 92-93 0.7937889902123842 1107 94-95 0.7945287937960899 1107 96-97 0.8820617617603013 1107 98-99 1.1253943218533848 1107 100-101 1.6072528024843145 1108 1 0.16672324616916612 1108 2 0.17743897281060583 1108 3 0.19285207622247214 1108 4 0.17090635124864662 1108 5 0.25509833087615874 1108 6 0.20210870951354565 1108 7 0.20741633047279606 1108 8 0.16933552615654435 1108 9 0.2249027088877824 1108 10-11 0.2950881856887193 1108 12-13 0.33459635917865427 1108 14-15 0.5003979052236716 1108 16-17 0.5453853849484105 1108 18-19 0.4857471571084986 1108 20-21 0.43840373524198384 1108 22-23 0.4913145168313875 1108 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1.3369858926724234 1108 92-93 1.6250657661521934 1108 94-95 1.6123724872737029 1108 96-97 1.6832400210040248 1108 98-99 1.8938077454242084 1108 100-101 2.33058712945612 1109 1 0.016948289053800636 1109 2 -0.03766453424789162 1109 3 0.2130511052660502 1109 4 0.21699906915465306 1109 5 0.22258281105263222 1109 6 0.08040839165457925 1109 7 -0.019110542644504847 1109 8 0.07444636903112212 1109 9 0.07015192671084947 1109 10-11 0.06913446031744286 1109 12-13 0.23935362403170046 1109 14-15 0.17450922435225635 1109 16-17 0.2069231331260326 1109 18-19 0.20625439533802847 1109 20-21 0.25519291623948703 1109 22-23 0.16215480625763945 1109 24-25 0.17808239468539 1109 26-27 0.22709979897857124 1109 28-29 0.2671211020527906 1109 30-31 0.27648113154513965 1109 32-33 0.21389327678255654 1109 34-35 0.37929356836409767 1109 36-37 0.26412760617734676 1109 38-39 0.46672906533749625 1109 40-41 0.3914029362773306 1109 42-43 0.4105347729196751 1109 44-45 0.3810766942316022 1109 46-47 0.4217606053783314 1109 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#Quality Count 2 18158.0 3 4798.0 4 4523.0 5 4965.0 6 5665.0 7 6671.0 8 8124.0 9 9231.0 10 11322.0 11 13480.0 12 16459.0 13 20000.0 14 21670.0 15 24574.0 16 29390.0 17 35812.0 18 32369.0 19 23991.0 20 23446.0 21 24557.0 22 26851.0 23 29580.0 24 33308.0 25 37994.0 26 42916.0 27 49761.0 28 57839.0 29 68179.0 30 80664.0 31 97099.0 32 118722.0 33 149014.0 34 196491.0 35 276100.0 36 390867.0 37 558617.0 38 490629.0 39 78820.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.78733048239001 18.76773610759212 14.311587745503696 22.13334566451417 2 15.453325100438642 20.393543058306538 40.24172634178809 23.911405499466735 3 19.45430983063051 25.316194138821572 29.747452174222737 25.48204385632518 4 12.720214932083154 16.799523741706857 37.2533547854849 33.22690654072509 5 13.50684567429266 37.64636171686571 33.856637257904126 14.990155350937503 6 31.02863970850117 36.23957271138703 17.71835299055183 15.013434589559974 7 28.09246647457923 33.41945809771806 20.706216647920165 17.781858779782542 8 26.366542247071184 34.4620235589312 19.784994977523255 19.386439216474365 9 26.924079752387755 16.933800412494655 20.513767834973915 35.62835200014368 10-11 24.931032192250512 26.790700152006746 26.924688790489004 21.353578865253734 12-13 26.30545786074656 24.37465472089896 27.526279720878904 21.79360769747558 14-15 23.038629459373627 25.499404935665687 26.566350475249497 24.895615129711192 16-17 21.739360128820795 28.97001814191933 27.131386483900773 22.159235245359103 18-19 21.590304520421864 27.144293522090145 30.049165896986736 21.216236060501256 20-21 22.58105711154064 26.091842051898112 30.06576464880599 21.261336187755248 22-23 22.14317964732434 26.196720295808024 30.00861762203389 21.651482434833746 24-25 21.99868944606066 26.403553356926302 30.16979906604977 21.427958130963273 26-27 21.737982726565217 26.484642658147557 30.2576073355865 21.519767279700723 28-29 21.420127410141994 26.70879512897619 30.326469616012393 21.544607844869432 30-31 21.6030146751359 26.455518222360393 30.205636249069435 21.735830853434273 32-33 21.408940154590667 26.701862550922485 30.12053913904465 21.768658155442196 34-35 22.066709282343645 26.589097135782996 29.81329769814281 21.53089588373055 36-37 22.036336056680867 26.37369420863051 29.887971568258187 21.701998166430435 38-39 21.758380192205756 26.69808163906887 29.95817658795405 21.585361580771323 40-41 21.836106534897418 26.57323478916374 30.045625037956604 21.545033637982243 42-43 21.696486185252535 26.748562194287988 29.562265806621664 21.99268581383781 44-45 21.78095076751069 26.870187487282095 29.42195037821813 21.926911366989085 46-47 22.031169805631155 27.11484078747987 28.88353123418214 21.970458172706834 48-49 22.191254243337468 27.135961235007144 28.650950274475505 22.021834247179882 50-51 22.020193219070354 27.34009977326596 28.661391424231862 21.978315583431826 52-53 22.21078339330681 27.44921331436817 28.458339870827686 21.88166342149734 54-55 21.882539598338184 27.242949377944203 28.600276004857818 22.274235018859795 56-57 22.04285340737801 27.592058036167018 28.353944246952317 22.011144309502654 58-59 22.098163895706385 27.177749838321596 28.45128559435391 22.27280067161811 60-61 21.986033633062355 27.16946165866549 28.92258973198819 21.92191497628397 62-63 21.911113939952696 27.250198822558986 28.850696312926456 21.987990924561867 64-65 21.708869612753332 27.291861951427354 28.73834923748503 22.260919198334285 66-67 21.653357508122895 27.486919034446984 28.41921670658905 22.44050675084107 68-69 21.630746421777587 27.318178382889496 28.63438213051567 22.41669306481724 70-71 21.763451829215935 27.277792482071572 28.452213528643515 22.506542160068975 72-73 21.825572733445465 27.13004682584205 28.503806378366885 22.540574062345595 74-75 21.658169023776463 27.55737999062344 28.28309884426483 22.501352141335264 76-77 21.820390269183758 27.281628592905143 28.32928664986693 22.568694488044173 78-79 21.84236654605812 27.260574568963992 28.371668894662356 22.52538999031553 80-81 21.779712532109734 27.530822288807087 28.17721196714561 22.512253211937573 82-83 21.813101150972493 27.393492487727627 28.24581942030388 22.547586940996002 84-85 21.777609103523126 27.268502622702485 28.181272528300628 22.772615745473765 86-87 21.918278324083104 27.21022193639355 28.21144563817744 22.66005410134591 88-89 21.941513281979784 27.101848846699223 28.035217995379046 22.921419875941943 90-91 21.887063483679643 27.14333973101441 28.180330032875943 22.789266752430002 92-93 22.018395679140973 27.088028241480348 28.135877073155672 22.757699006223003 94-95 21.792934376766777 26.842061593617967 28.21547539490551 23.14952863470975 96-97 21.89487039096174 26.67263235671289 28.221605285183298 23.21089196714207 98-99 21.90913033320186 26.70779598857259 27.9273970133539 23.455676664871657 100-101 22.191836754377643 26.15839416410403 28.388955779070574 23.26081330244775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5868.0 1 3724.5 2 1907.5 3 2203.5 4 1831.0 5 1781.5 6 2850.5 7 5196.0 8 7899.0 9 8641.5 10 6947.0 11 5216.5 12 4851.5 13 5030.0 14 5255.5 15 5568.5 16 5902.0 17 6211.0 18 6546.5 19 6978.5 20 7583.0 21 8492.5 22 9704.0 23 11377.0 24 13466.0 25 16119.0 26 19776.5 27 23852.0 28 27586.5 29 31911.5 30 37965.0 31 44509.0 32 51666.0 33 57583.0 34 63408.0 35 68997.0 36 74992.0 37 80861.0 38 83955.0 39 85871.0 40 86539.0 41 89717.5 42 93401.5 43 98012.5 44 103216.0 45 106509.5 46 107645.0 47 111146.0 48 124801.0 49 125238.0 50 114422.5 51 109348.5 52 103356.0 53 100814.5 54 98712.5 55 92656.5 56 84599.0 57 75904.5 58 68478.0 59 60607.0 60 50866.0 61 41648.0 62 34619.0 63 27850.5 64 21527.5 65 17095.5 66 13395.5 67 10933.5 68 8317.0 69 5712.0 70 4289.0 71 3252.0 72 2507.5 73 2046.5 74 1651.5 75 1378.5 76 1018.5 77 629.5 78 420.0 79 310.0 80 228.5 81 181.0 82 138.0 83 97.0 84 69.5 85 51.0 86 38.0 87 34.5 88 28.0 89 21.5 90 16.5 91 14.0 92 11.5 93 7.5 94 3.5 95 1.0 96 3.0 97 5.0 98 3.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27671583995634547 2 0.16405685083860175 3 0.19281403453039647 4 0.16210341185731056 5 0.23377220595779674 6 0.19585627556683358 7 0.23857574443638163 8 0.24661366549054695 9 0.1472124425736975 10-11 0.20459871559785797 12-13 0.21005873766851615 14-15 0.17254310215076835 16-17 0.2854903035772271 18-19 0.23138644851343296 20-21 0.1963046058248348 22-23 0.2424025634243209 24-25 0.23125835415400403 26-27 0.34060290172152413 28-29 0.3125182134167313 30-31 0.28808421435566295 32-33 0.19057238324039022 34-35 0.3576074279357146 36-37 0.396179841918751 38-39 0.3766294403109106 40-41 0.33916184017794865 42-43 0.37213012593596945 44-45 0.3819453562272112 46-47 0.387213236758726 48-49 0.40591501323534973 50-51 0.3985655993631148 52-53 0.39818131628482806 54-55 0.3921448695967398 56-57 0.37158572490839653 58-59 0.3461910181516112 60-61 0.31125328161737065 62-63 0.3805683418633502 64-65 0.41384085172501467 66-67 0.4202295579015326 68-69 0.4247288722764737 70-71 0.42434458919818696 72-73 0.4157782789113773 74-75 0.4104143276102908 76-77 0.3949149101193904 78-79 0.36926401464374714 80-81 0.3804722710937785 82-83 0.3892307229197316 84-85 0.38959899420308974 86-87 0.38602836393400836 88-89 0.3938741434490303 90-91 0.4155861373722339 92-93 0.414577394291731 94-95 0.42335185791261276 96-97 0.4243285774032583 98-99 0.4213183599566785 100-101 0.39366599011495834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3122698.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.21359452865378 #Duplication Level Percentage of deduplicated Percentage of total 1 73.93715037827299 37.126500896987 2 14.099600404987317 14.159832355041516 3 5.019791002531247 7.561852500590654 4 2.3379192027763374 4.695813075558579 5 1.208447613164499 3.0340249228280807 6 0.7504008495712206 2.260819439659595 7 0.5108769102683008 1.7957076218384729 8 0.35585425459806624 1.4294977001346902 9 0.24922209296688205 1.1262903411439324 >10 1.336935137802511 12.649223017137121 >50 0.12228061709643341 4.271851146281192 >100 0.0661492816631717 5.943472527691633 >500 0.0038373245011270765 1.311227253879807 >1k 0.0013430635749317537 1.2993770733622239 >5k 6.395540833008351E-5 0.2930751877487866 >10k+ 1.2791081666016702E-4 1.0414349401167433 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18118 0.5802034010333372 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14311 0.4582895944468533 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9126 0.2922472810371032 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4234 0.13558787945552211 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4094 0.13110457687550955 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3661 0.11723836246732794 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.202358985723243E-5 0.0 9 0.0 0.0 0.0 1.2809435942892972E-4 0.0 10-11 0.0 0.0 0.0 1.2809435942892972E-4 0.0 12-13 0.0 0.0 0.0 1.921415391433946E-4 0.0 14-15 0.0 0.0 0.0 2.7220051378647566E-4 0.0 16-17 0.0 0.0 0.0 3.842830782867892E-4 0.0 18-19 0.0 0.0 0.0 4.48330258001254E-4 0.0 20-21 0.0 0.0 0.0 5.444010275729513E-4 0.0 22-23 0.0 0.0 0.0 8.005897464308108E-4 0.0 24-25 0.0 0.0 0.0 0.0012809435942892971 0.0 26-27 0.0 0.0 0.0 0.002081533340720108 0.0 28-29 0.0 0.0 0.0 0.0042431256560832974 0.0 30-31 0.0 0.0 0.0 0.010295584139100226 0.0 32-33 0.0 0.0 0.0 0.023153055466779048 0.0 34-35 0.0 0.0 0.0 0.043904341694265665 0.0 36-37 0.0 0.0 0.0 0.0803792105416534 0.0 38-39 0.0 0.0 0.0 0.13531567894173563 0.0 40-41 0.0 0.0 0.0 0.1946233673573301 0.0 42-43 0.0 0.0 0.0 0.2534026665402802 0.0 44-45 0.0 0.0 0.0 0.32913845655263496 0.0 46-47 0.0 0.0 0.0 0.4259777922809058 0.0 48-49 0.0 0.0 0.0 0.532023910093131 0.0 50-51 0.0 0.0 0.0 0.6444747458768026 0.0 52-53 0.0 0.0 0.0 0.7575980770474762 0.0 54-55 0.0 0.0 0.0 0.8856764246814774 0.0 56-57 0.0 0.0 0.0 1.0409588118991975 0.0 58-59 0.0 0.0 0.0 1.2161918955979734 0.0 60-61 0.0 0.0 0.0 1.4123203716785933 0.0 62-63 0.0 0.0 0.0 1.60420572210313 0.0 64-65 0.0 0.0 0.0 1.8080839069292005 0.0 66-67 0.0 0.0 0.0 2.045859061619151 0.0 68-69 0.0 0.0 0.0 2.3151934641134044 0.0 70-71 0.0 0.0 0.0 2.609890549774586 0.0 72-73 0.0 0.0 0.0 2.9149632785495108 0.0 74-75 0.0 0.0 0.0 3.2196197006562914 0.0 76-77 0.0 0.0 0.0 3.54640442335442 0.0 78-79 0.0 0.0 0.0 3.9280615672729158 0.0 80-81 0.0 0.0 0.0 4.345793285165584 0.0 82-83 0.0 0.0 0.0 4.77489337745757 0.0 84-85 0.0 0.0 0.0 5.199558202554329 0.0 86-87 0.0 0.0 0.0 5.63994661027099 0.0 88-89 0.0 0.0 0.0 6.121949673007125 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15930 0.0 29.23894 1 GTATCAA 22580 0.0 27.973856 1 GTACATG 16255 0.0 25.350317 1 TACATGG 16585 0.0 24.512806 2 TATCAAC 26675 0.0 23.474545 2 ATCAACG 26700 0.0 23.471087 3 TCAACGC 26815 0.0 23.370426 4 GAGTACT 14090 0.0 23.328167 12-13 ACATGGG 16660 0.0 23.121855 3 CAACGCA 27270 0.0 23.015284 5 AACGCAG 27740 0.0 22.692648 6 GTACTTT 15230 0.0 21.489367 14-15 GTGTAGC 1410 0.0 20.56184 1 ACGCAGA 30505 0.0 20.433935 7 CGCAGAG 30725 0.0 20.210424 8 AGTACTT 14875 0.0 19.911293 12-13 TACCTGG 2295 0.0 19.636703 2 CATGGGG 7105 0.0 19.630207 4 GCAGAGT 31470 0.0 19.128399 9 ACTTTTT 17855 0.0 18.742714 16-17 >>END_MODULE