FastQCFastQC Report
Thu 26 May 2016
SRR938680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122698
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172030.5509018163139695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152530.4884558160923663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99570.3188588842084633No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46730.14964623540284716No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44480.14244092768496985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38460.12316272659091594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185900.026.4110161
GTATCAA252850.025.5329911
GAGTACT155950.023.4564912-13
TACCTGG20950.022.881862
GTACATG161600.022.1097281
GTACTTT167300.021.68081314-15
ATCAACG297550.021.5660573
TATCAAC299150.021.4665762
TCAACGC299600.021.4501744
TACATGG164300.021.232632
CAACGCA305650.021.0100655
AACGCAG308650.020.8138926
AGTACTT163500.020.74828712-13
ACATGGG163450.020.3847873
GTACCTG26050.019.1763691
ACGCAGA338400.018.9407947
ACTTTTT193150.018.91383416-17
CGCAGAG340800.018.7795648
GCAGAGT349000.018.0664449
TACTTTT187500.018.05455214-15
Produced by FastQC (version 0.11.5)