##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938680_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3122698 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05804179590854 33.0 31.0 34.0 30.0 34.0 2 32.269572017531 34.0 31.0 34.0 30.0 34.0 3 32.33574236125299 34.0 31.0 34.0 30.0 34.0 4 35.95813652168734 37.0 35.0 37.0 35.0 37.0 5 35.865842294067505 37.0 35.0 37.0 35.0 37.0 6 35.90001370609646 37.0 35.0 37.0 35.0 37.0 7 35.82189728241412 37.0 35.0 37.0 33.0 37.0 8 35.80801249432382 37.0 35.0 37.0 33.0 37.0 9 37.450066577043316 39.0 37.0 39.0 34.0 39.0 10-11 37.37603876519599 39.0 37.0 39.0 34.0 39.0 12-13 37.293689303288375 39.0 37.0 39.0 34.0 39.0 14-15 38.49715758616427 40.0 38.0 41.0 34.0 41.0 16-17 38.42569534421837 40.0 38.0 41.0 33.0 41.0 18-19 38.58785271582458 40.0 38.0 41.0 34.0 41.0 20-21 38.59936455590646 40.0 38.0 41.0 34.0 41.0 22-23 38.52554633845476 40.0 38.0 41.0 34.0 41.0 24-25 38.44532852680599 40.0 38.0 41.0 34.0 41.0 26-27 38.26512041830494 40.0 38.0 41.0 33.5 41.0 28-29 38.088676522673666 40.0 37.5 41.0 33.0 41.0 30-31 37.84522886939435 40.0 37.0 41.0 33.0 41.0 32-33 37.65541640594127 40.0 37.0 41.0 33.0 41.0 34-35 37.60077727657301 40.0 37.0 41.0 32.5 41.0 36-37 37.469174252521384 40.0 36.0 41.0 32.0 41.0 38-39 37.33943532163533 40.0 36.0 41.0 32.0 41.0 40-41 37.14930550440677 40.0 36.0 41.0 31.0 41.0 42-43 36.92783131766184 40.0 36.0 41.0 31.0 41.0 44-45 36.63965791760843 39.5 35.0 41.0 30.0 41.0 46-47 36.270200960835794 39.0 35.0 41.0 30.0 41.0 48-49 35.9206842928775 39.0 35.0 41.0 28.5 41.0 50-51 35.6605364976056 39.0 35.0 41.0 28.0 41.0 52-53 35.42815267438606 39.0 35.0 40.0 27.0 41.0 54-55 35.14827114245438 38.0 34.5 40.0 26.5 41.0 56-57 34.87526507526504 38.0 34.0 40.0 26.0 41.0 58-59 34.56423099512024 38.0 34.0 40.0 26.0 41.0 60-61 34.19729333416168 37.5 33.5 40.0 25.0 41.0 62-63 33.83814044777945 37.0 33.0 40.0 23.5 41.0 64-65 33.438859601536876 36.0 33.0 39.0 23.0 41.0 66-67 33.032030635047 36.0 32.0 39.0 22.0 40.0 68-69 32.69442145862328 35.5 32.0 39.0 21.5 40.0 70-71 32.26253275212653 35.0 32.0 38.0 20.0 40.0 72-73 31.775980578333225 35.0 31.0 37.0 18.5 39.5 74-75 31.280394709959147 35.0 31.0 37.0 17.5 39.0 76-77 29.373801116854718 32.5 28.5 35.0 12.5 37.5 78-79 30.314469090510833 34.0 30.0 36.0 11.0 38.0 80-81 30.363150551222052 34.0 30.0 35.5 9.5 37.0 82-83 30.039462669781066 34.0 30.0 35.0 7.0 37.0 84-85 29.634277794394464 34.0 30.0 35.0 2.0 36.5 86-87 29.043608123488085 34.0 29.0 35.0 2.0 36.0 88-89 28.432857099854036 34.0 28.5 35.0 2.0 35.5 90-91 27.907248955870855 33.0 27.0 35.0 2.0 35.0 92-93 27.60401277997424 33.0 27.0 35.0 2.0 35.0 94-95 27.2427998160565 33.0 26.5 35.0 2.0 35.0 96-97 26.612801974446455 33.0 25.0 35.0 2.0 35.0 98-99 25.76009399564095 33.0 24.0 34.5 2.0 35.0 100-101 22.09960937625092 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03207482889614255 1101 2 0.15760595633667407 1101 3 0.06703901103648491 1101 4 0.05948890492956593 1101 5 -0.011709211436667033 1101 6 0.1175412285309676 1101 7 0.14601281533932564 1101 8 0.1854671350717254 1101 9 0.3016581057785501 1101 10-11 0.2603536164234228 1101 12-13 0.13185531114947224 1101 14-15 0.28594590963668765 1101 16-17 0.31346293373957934 1101 18-19 0.34755553405232575 1101 20-21 0.22026599416931703 1101 22-23 0.1867257749118707 1101 24-25 0.16159368069553892 1101 26-27 0.0966355378824133 1101 28-29 0.16627351743226626 1101 30-31 0.25016527333136196 1101 32-33 0.2889380144573437 1101 34-35 0.3056741050141838 1101 36-37 0.3462378669495507 1101 38-39 0.33627443984209293 1101 40-41 0.3317107995432167 1101 42-43 0.3889074593152628 1101 44-45 0.34499220234970807 1101 46-47 0.41139738616187316 1101 48-49 0.3612376740562411 1101 50-51 0.30150415551973 1101 52-53 0.2164531841904065 1101 54-55 0.3961676103710303 1101 56-57 0.2142298627892245 1101 58-59 0.281676724968392 1101 60-61 0.2167947051797796 1101 62-63 0.24967377712297179 1101 64-65 0.18502905562858984 1101 66-67 0.16183680722293303 1101 68-69 0.29852855129941247 1101 70-71 0.3881093691784159 1101 72-73 0.2726596493955036 1101 74-75 0.18784463341873803 1101 76-77 0.23114081428322208 1101 78-79 0.06906387453247831 1101 80-81 0.19877510453868652 1101 82-83 0.33807225880009284 1101 84-85 0.41348743674604904 1101 86-87 0.5089694208819857 1101 88-89 0.3640301333415259 1101 90-91 0.34335184310841527 1101 92-93 0.5170072580049379 1101 94-95 0.5418376794787925 1101 96-97 0.38968141784814847 1101 98-99 0.2710224986223082 1101 100-101 1.0655694507012683 1102 1 0.2928786802172354 1102 2 0.1802716527092727 1102 3 0.26004729585510944 1102 4 0.17471505808675403 1102 5 -0.00955403902287344 1102 6 -5.39828343192994E-4 1102 7 0.13747049559010804 1102 8 0.1546453706292681 1102 9 0.23145546359315006 1102 10-11 0.24211581973735719 1102 12-13 0.2234249484087698 1102 14-15 0.38761406906346707 1102 16-17 0.3945953788806449 1102 18-19 0.3645506079439471 1102 20-21 0.3605425279803285 1102 22-23 0.26603891866466967 1102 24-25 0.29730796640982504 1102 26-27 0.2519954796647639 1102 28-29 0.3541989540646 1102 30-31 0.3296295590456495 1102 32-33 0.4214111447753055 1102 34-35 0.43809125682788874 1102 36-37 0.3305694836087554 1102 38-39 0.30324992125722616 1102 40-41 0.29636597195700887 1102 42-43 0.36359397969144425 1102 44-45 0.3093758790178711 1102 46-47 0.31018265261955946 1102 48-49 0.3135159991794012 1102 50-51 0.29458241346867453 1102 52-53 0.12885242288275123 1102 54-55 0.05853885981124307 1102 56-57 0.04663861782728418 1102 58-59 0.16889123459669975 1102 60-61 0.15903328556491658 1102 62-63 0.10382178428822186 1102 64-65 0.201083690648737 1102 66-67 0.29921085558835614 1102 68-69 0.31068148457303835 1102 70-71 0.46876319810810685 1102 72-73 0.36315170044789724 1102 74-75 0.33969192405912807 1102 76-77 0.27415469695227657 1102 78-79 0.23551595693284 1102 80-81 0.4470761569345676 1102 82-83 0.497121184012812 1102 84-85 0.6661184264460047 1102 86-87 0.6315987312268128 1102 88-89 0.6328752967987619 1102 90-91 0.6778989770090007 1102 92-93 0.7483244322100404 1102 94-95 0.7445918218881076 1102 96-97 0.7415063170868414 1102 98-99 1.0466802460472948 1102 100-101 1.5192332438047131 1103 1 0.17115780657488244 1103 2 0.13580551609003777 1103 3 0.09683923435560615 1103 4 0.041134125793732323 1103 5 -0.06747604535056695 1103 6 -0.04749974074764651 1103 7 -0.03933886386361962 1103 8 0.04487757645141954 1103 9 0.09972115614737476 1103 10-11 0.19112919038554566 1103 12-13 0.13332886875098637 1103 14-15 0.1980545806360965 1103 16-17 0.08045852643106599 1103 18-19 -0.08782045265312632 1103 20-21 0.03747144486043652 1103 22-23 0.1141736420980024 1103 24-25 0.09707885747693723 1103 26-27 0.13443462426895536 1103 28-29 0.03511464030329847 1103 30-31 0.20194427261763792 1103 32-33 0.26961040258714775 1103 34-35 0.1715422072761399 1103 36-37 0.3024586507357654 1103 38-39 0.17222358970898455 1103 40-41 0.19941450561418606 1103 42-43 0.2970080071540764 1103 44-45 0.34682954932623034 1103 46-47 0.3289080848524648 1103 48-49 0.2990579205594983 1103 50-51 0.3292243442079297 1103 52-53 0.28326802009539875 1103 54-55 0.19955594063929283 1103 56-57 0.1633696096231958 1103 58-59 0.2851128470792119 1103 60-61 0.32537157354061463 1103 62-63 0.28067746392225246 1103 64-65 0.34444835810979413 1103 66-67 0.34080984913739343 1103 68-69 0.3165647181515254 1103 70-71 0.3396917779066513 1103 72-73 0.41945987174620925 1103 74-75 0.4773016657922611 1103 76-77 0.5102563601416001 1103 78-79 0.43544403225040185 1103 80-81 0.471045328429188 1103 82-83 0.5002396164389928 1103 84-85 0.43934490568619466 1103 86-87 0.5723294924680609 1103 88-89 0.491209020096651 1103 90-91 0.4822529503103148 1103 92-93 0.39064480312149996 1103 94-95 0.5177260412209144 1103 96-97 0.829739697748554 1103 98-99 0.8156429384234727 1103 100-101 1.450895902396642 1104 1 0.08878290820840817 1104 2 0.08747306405442856 1104 3 0.07805214640967506 1104 4 0.056081977606837086 1104 5 -0.046477563456974735 1104 6 0.04182243706222977 1104 7 0.021323890978301563 1104 8 0.024690551807303507 1104 9 0.04211043956703975 1104 10-11 0.08303954061135954 1104 12-13 -0.012738126933491856 1104 14-15 0.019425244982954837 1104 16-17 0.17604635201371366 1104 18-19 0.20653057107369222 1104 20-21 0.18425622437489864 1104 22-23 0.14189097675043882 1104 24-25 0.16076863309663736 1104 26-27 0.09003323390333406 1104 28-29 0.045889168335200736 1104 30-31 -0.026008832867070453 1104 32-33 0.06812190968206977 1104 34-35 0.10166714307407432 1104 36-37 0.20838424077880546 1104 38-39 0.18207275512951782 1104 40-41 0.1753113918850815 1104 42-43 0.1135093594248957 1104 44-45 0.277868618296786 1104 46-47 0.1887975246314042 1104 48-49 0.37718330553498447 1104 50-51 0.28718890656305973 1104 52-53 0.2710548919812439 1104 54-55 0.32626982780664804 1104 56-57 0.22400061879437771 1104 58-59 0.4219723189449667 1104 60-61 0.49851336202537055 1104 62-63 0.5228602109556917 1104 64-65 0.34104863368116867 1104 66-67 0.3183862235354056 1104 68-69 0.3993170583286627 1104 70-71 0.28549912243341424 1104 72-73 0.3570380750739126 1104 74-75 0.39058950331507347 1104 76-77 0.4928905306130069 1104 78-79 0.4286976034014529 1104 80-81 0.4645870744600309 1104 82-83 0.336952963456131 1104 84-85 0.4091410321457829 1104 86-87 0.5623224989441802 1104 88-89 0.509712576559405 1104 90-91 0.5613951917631468 1104 92-93 0.6075047867085139 1104 94-95 0.5118777779460117 1104 96-97 0.5717255742417855 1104 98-99 0.7928574121750103 1104 100-101 1.5526965926517633 1105 1 0.17609060768814544 1105 2 0.15555071150813404 1105 3 0.11489086571152285 1105 4 0.06434926833725285 1105 5 0.0195348929549084 1105 6 0.07601137174065542 1105 7 0.12539366926163353 1105 8 0.16504522389412557 1105 9 0.2272224167427197 1105 10-11 0.18753820732775495 1105 12-13 0.16525756553485138 1105 14-15 0.1759774481641898 1105 16-17 0.20982504558224946 1105 18-19 0.20535345879815736 1105 20-21 0.16953136562838012 1105 22-23 0.14125836631166777 1105 24-25 0.18107277093765362 1105 26-27 0.21757626260156826 1105 28-29 0.2532674479331689 1105 30-31 0.23104778040609375 1105 32-33 0.2611436151662474 1105 34-35 0.2982434945388164 1105 36-37 0.18941944692496548 1105 38-39 0.31886345757417445 1105 40-41 0.24371163802975104 1105 42-43 0.23799655803752273 1105 44-45 0.2809570811979327 1105 46-47 0.16664030904970417 1105 48-49 0.24153848109953202 1105 50-51 0.09622073754372451 1105 52-53 0.18501660902676065 1105 54-55 0.2018702716129397 1105 56-57 0.17975425036056691 1105 58-59 0.2540447824428327 1105 60-61 0.18197178781834822 1105 62-63 0.17889122145413694 1105 64-65 0.19546059034478702 1105 66-67 0.1957278611433324 1105 68-69 0.21063038930000744 1105 70-71 0.1078838353578746 1105 72-73 0.3117059662481303 1105 74-75 0.26598109710178974 1105 76-77 0.23594067326503065 1105 78-79 0.0245592962054495 1105 80-81 0.0964073329119266 1105 82-83 0.08959249205180342 1105 84-85 0.2282424176418978 1105 86-87 0.2555525882648588 1105 88-89 0.15854805122701876 1105 90-91 0.179704998390406 1105 92-93 0.4328222573855953 1105 94-95 0.5451944840828453 1105 96-97 0.592813832022383 1105 98-99 0.7719481686969161 1105 100-101 1.4225970159459997 1106 1 0.04957426707056811 1106 2 0.015553407553078102 1106 3 0.03562991441391006 1106 4 0.03638149530730317 1106 5 -0.03386323163191207 1106 6 -0.05611791284720624 1106 7 0.004823954340686498 1106 8 -0.03117680463962813 1106 9 8.041309102466698E-4 1106 10-11 -0.013906392641963805 1106 12-13 -0.002558028684525482 1106 14-15 -0.014598489819391602 1106 16-17 0.017603873873731857 1106 18-19 -0.038152766111082315 1106 20-21 -0.06408554485185647 1106 22-23 -0.07908513935926464 1106 24-25 -0.03241059298778737 1106 26-27 0.021642489283543398 1106 28-29 -0.009256788772191271 1106 30-31 0.022603158277107127 1106 32-33 0.03350592298071575 1106 34-35 0.10782913459096477 1106 36-37 0.005380863936636615 1106 38-39 0.01938872468576136 1106 40-41 0.16189683046457048 1106 42-43 0.16163625563731188 1106 44-45 0.236335136869414 1106 46-47 0.10500133531952116 1106 48-49 0.14574661946203804 1106 50-51 0.10805637482596353 1106 52-53 0.047025827284521426 1106 54-55 0.19450812999301803 1106 56-57 0.13627018472479335 1106 58-59 0.15572676362961602 1106 60-61 0.08164927805903233 1106 62-63 0.10885600667538142 1106 64-65 0.1744173132837048 1106 66-67 0.17888164280801533 1106 68-69 0.20522094132444124 1106 70-71 0.21563948874251793 1106 72-73 0.12163341309357278 1106 74-75 0.4339291853104683 1106 76-77 0.3016321164615796 1106 78-79 0.1727368806230487 1106 80-81 0.18907104377772654 1106 82-83 0.30214018065492 1106 84-85 0.2966662839180678 1106 86-87 0.3925094552390469 1106 88-89 0.4912577241192082 1106 90-91 0.35825279040346913 1106 92-93 0.3118833135704371 1106 94-95 0.6209460527747446 1106 96-97 0.7390926436944092 1106 98-99 0.7705475179529166 1106 100-101 1.250500527082881 1107 1 0.028940511838001726 1107 2 0.007973727066250547 1107 3 -0.05484102334948204 1107 4 -0.008514035319549862 1107 5 -0.06637982782520169 1107 6 -0.05492374100755626 1107 7 0.047190448362115944 1107 8 0.039899311440670715 1107 9 0.06733414744422106 1107 10-11 -0.060251942640796585 1107 12-13 0.017753711550447804 1107 14-15 -0.03907241900228087 1107 16-17 -0.08449628611687388 1107 18-19 -0.06125807351259027 1107 20-21 -0.05214846978628174 1107 22-23 0.050467414417013856 1107 24-25 0.006272625234274187 1107 26-27 -0.04239535869231048 1107 28-29 0.01249489480672139 1107 30-31 0.01716735009793524 1107 32-33 -0.014034866823863013 1107 34-35 -0.07350254856447691 1107 36-37 -0.10120340713284293 1107 38-39 -0.06633287493801987 1107 40-41 -0.3032384409084017 1107 42-43 -0.33657021137108245 1107 44-45 -0.3044887497619939 1107 46-47 -0.2632902123695331 1107 48-49 -0.37500242967666964 1107 50-51 -0.3858635548331506 1107 52-53 -0.4175441649852587 1107 54-55 -0.3716565956994131 1107 56-57 -0.4018964165018559 1107 58-59 -0.4331158044221155 1107 60-61 -0.3265160843269399 1107 62-63 -0.3823954471515236 1107 64-65 -0.48442096061561557 1107 66-67 -0.6296012026845972 1107 68-69 -0.567671330055596 1107 70-71 -0.5711746494942744 1107 72-73 -0.5063683145969868 1107 74-75 -0.4247582824880318 1107 76-77 -0.47964605694862783 1107 78-79 -0.33354396734575076 1107 80-81 -0.3418271194320077 1107 82-83 -0.3218157663932786 1107 84-85 -0.2863977517011129 1107 86-87 -0.20162841379896435 1107 88-89 -0.2987776320595614 1107 90-91 -0.08471066881118716 1107 92-93 0.13742162420778925 1107 94-95 0.2654468352688717 1107 96-97 0.3351789936760028 1107 98-99 0.6600722748931176 1107 100-101 1.550660480545563 1108 1 0.11069936631565014 1108 2 0.013063557368781176 1108 3 0.005483835227600764 1108 4 -0.02311580518665579 1108 5 -0.05132986714097143 1108 6 0.022945074031909485 1108 7 0.009782719032045861 1108 8 0.06346810832976502 1108 9 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0.22530538993553506 2206 18-19 0.09077803500899506 2206 20-21 0.18557267078281114 2206 22-23 0.22711806895652842 2206 24-25 0.22780716766352072 2206 26-27 0.22430301369499972 2206 28-29 0.1630347433656496 2206 30-31 0.25681627890132575 2206 32-33 0.15858982401944388 2206 34-35 0.11896287734139577 2206 36-37 0.4129599369200534 2206 38-39 0.4447971429564035 2206 40-41 0.43088487157008615 2206 42-43 0.4576934311987344 2206 44-45 0.5385388126338526 2206 46-47 0.6068550238938499 2206 48-49 0.5641268275066409 2206 50-51 0.6142086806812088 2206 52-53 0.568681846509854 2206 54-55 0.6835077299648518 2206 56-57 0.7054754321354721 2206 58-59 0.6861493177270503 2206 60-61 0.790023313384367 2206 62-63 0.7222902110313427 2206 64-65 0.6126346858927079 2206 66-67 0.6313099411301408 2206 68-69 0.44262230142020087 2206 70-71 0.5783440537081432 2206 72-73 0.5117779251416117 2206 74-75 0.40243071275884645 2206 76-77 0.4827368159685115 2206 78-79 0.6716265028136768 2206 80-81 0.5419604981662793 2206 82-83 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-1.688790345730542 2316 98-99 -1.7572618283267083 2316 100-101 -1.9256717239636245 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 17.0 4 39.0 5 89.0 6 269.0 7 599.0 8 1376.0 9 2404.0 10 4070.0 11 6065.0 12 8395.0 13 11571.0 14 14303.0 15 19236.0 16 24812.0 17 33778.0 18 32137.0 19 21373.0 20 19450.0 21 20211.0 22 21029.0 23 23321.0 24 27382.0 25 32166.0 26 38546.0 27 46983.0 28 56464.0 29 69261.0 30 81931.0 31 99719.0 32 121930.0 33 154149.0 34 204059.0 35 287111.0 36 420081.0 37 640065.0 38 527045.0 39 51257.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.815288517306804 19.935151450763218 15.122608501117133 22.12695153081285 2 15.945121814533458 21.18434123312597 39.23242016999403 23.638116782346547 3 19.518858371831023 25.315000041630665 30.1436450146636 25.022496571874708 4 13.152056330775503 17.48267043434876 36.69762493843465 32.667648296441094 5 13.337621518145557 37.9342587953703 33.990997374554574 14.737122311929568 6 30.98412887510972 36.8243787446891 17.251133594590897 14.940358785610275 7 27.969147192587947 33.52258207485963 20.492535621440176 18.01573511111225 8 26.377126446425496 34.975812582580836 19.448438497734973 19.198622473258702 9 26.519087020262607 16.917261931829465 20.384295887722732 36.1793551601852 10-11 24.96587886500712 27.071125674016507 26.68639426547172 21.276601195504657 12-13 26.203862813502937 24.500431998227175 27.53184906129251 21.763856126977377 14-15 22.796712970642695 25.82537600498031 26.42448933582434 24.953421688552655 16-17 21.567602976434188 29.182950529455383 27.12786526464923 22.121581229461196 18-19 21.456685853066805 27.248536361825575 30.1013418524622 21.193435932645425 20-21 22.383720744048897 26.208169986338735 30.16759865987681 21.240510609735555 22-23 22.040203695650366 26.293672971257546 30.06912612106582 21.596997212026267 24-25 21.911243803017207 26.501070071339157 30.25296467243456 21.334721453209077 26-27 21.665751310187357 26.578589625141507 30.34043254996966 21.41522651470148 28-29 21.342457708046055 26.843005631668515 30.390803081181723 21.42373357910371 30-31 21.51818715738762 26.55815579988843 30.24722531605682 21.67643172666713 32-33 21.37547402918886 26.740113837457223 30.17398736605333 21.710424767300584 34-35 22.02080711227258 26.60873213364975 29.901937235987948 21.46852351808973 36-37 21.991724967594582 26.42305991070934 29.97370733046289 21.61150779123319 38-39 21.699489505541965 26.76452718489482 30.074590033459664 21.46139327610355 40-41 21.806621765740143 26.57150040193055 30.142453153473415 21.479424678855892 42-43 21.663532638119705 26.817601780399368 29.619171895874562 21.899693685606362 44-45 21.8139851036285 26.942016558462612 29.391551357094194 21.852446980814697 46-47 22.019632805971405 27.167612194839595 28.900257679177336 21.912497320011664 48-49 22.134111349228412 27.25800635615203 28.633891663603023 21.973990631016534 50-51 21.984432682528173 27.455906690885058 28.60387306353228 21.95578756305449 52-53 22.23407450864605 27.52502803665292 28.350179876504228 21.890717578196803 54-55 21.896289682831963 27.314953287189475 28.49515066778792 22.293606362190644 56-57 21.991581852450402 27.722387830753824 28.25073571125373 22.035294605542042 58-59 22.13885921360059 27.312815113133297 28.29701526377275 22.25131040949336 60-61 21.962108995522822 27.299383257203218 28.751964099314854 21.986543647959106 62-63 21.897345171285394 27.32544296648844 28.727513022916135 22.049698839310032 64-65 21.71365941373419 27.383900507422393 28.592315168711142 22.310124910132277 66-67 21.677837711812977 27.562900579566357 28.24518888188241 22.51407282673826 68-69 21.62810641725769 27.424232768617046 28.519746923244675 22.427913890880586 70-71 21.71083896735385 27.42820626791721 28.284421538311765 22.576533226417173 72-73 21.78889857424573 27.288117518889116 28.287637805513054 22.6353461013521 74-75 21.63428267660683 27.687374807257214 28.056560299870142 22.62178221626581 76-77 21.703363736524476 27.4652146447351 28.244303426955682 22.587118191784747 78-79 21.67550063378469 27.420676694442015 28.2408526147928 22.66297005698049 80-81 21.61889396538666 27.707723745296093 28.045944030571285 22.627438258745965 82-83 21.6303598222865 27.617518553454236 28.08191332631903 22.67020829794024 84-85 21.564877551399462 27.534010653607872 27.970380100797453 22.93073169419521 86-87 21.677245978702036 27.486998521856236 27.981828865509435 22.853926633932296 88-89 21.62229176395421 27.433945442700757 27.8135878059355 23.130174987409532 90-91 21.57633399610567 27.452534939418943 27.922407250854747 23.04872381362064 92-93 21.626093689855463 27.44947778931343 27.912204429135684 23.01222409169542 94-95 21.37482084759921 27.278207686608713 27.912916762003793 23.434054703788288 96-97 21.479539308193722 27.13881759185575 27.8851600940854 23.496483005865127 98-99 21.369821865579063 27.2620343049504 27.579356056845718 23.788787772624826 100-101 21.52370884663045 26.682606050892133 28.219739334705775 23.573945767771647 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3775.0 1 2418.0 2 1374.5 3 1668.5 4 1364.0 5 1223.0 6 2261.5 7 4822.0 8 7234.5 9 7848.5 10 6372.0 11 4594.0 12 4237.5 13 4415.5 14 4744.0 15 5161.0 16 5434.0 17 5807.5 18 6289.0 19 6765.5 20 7423.5 21 8410.0 22 9701.0 23 11479.0 24 13549.0 25 16088.0 26 19940.5 27 24544.0 28 28299.0 29 32368.0 30 38527.0 31 45310.0 32 52781.0 33 58784.0 34 64288.5 35 69490.5 36 75307.5 37 81329.0 38 84496.0 39 86215.0 40 87153.0 41 90372.5 42 93943.5 43 98909.0 44 103925.5 45 106792.0 46 108078.0 47 112308.5 48 126631.5 49 126881.5 50 115747.0 51 110091.5 52 103634.5 53 101619.5 54 99743.5 55 93220.0 56 84878.5 57 75634.0 58 67925.5 59 59904.5 60 50113.0 61 41032.5 62 33943.0 63 27134.5 64 20824.5 65 16519.0 66 12790.5 67 10432.0 68 7653.0 69 4984.5 70 3723.0 71 2859.0 72 2252.5 73 1855.0 74 1458.5 75 1169.5 76 837.5 77 502.5 78 328.5 79 216.0 80 130.5 81 87.5 82 68.0 83 46.5 84 27.5 85 21.5 86 15.0 87 6.5 88 6.5 89 5.0 90 5.0 91 3.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22836021927192446 2 0.0 3 0.0 4 0.0 5 0.03016622164551295 6 0.0034905712944383353 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0032824179603663244 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.882123087150919E-4 26-27 0.001697250262433319 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006660906690304346 36-37 0.01713262057361935 38-39 0.013802167228467178 40-41 0.008582322081738291 42-43 0.008085956438951188 44-45 0.00401896052708267 46-47 5.283892326443351E-4 48-49 0.0017292738522905512 50-51 6.404717971446486E-5 52-53 0.0 54-55 0.0 56-57 0.0012649317993606811 58-59 3.2023589857232434E-4 60-61 0.002673969753078908 62-63 9.446959007883566E-4 64-65 0.001697250262433319 66-67 0.006116505662731395 68-69 2.882123087150919E-4 70-71 6.404717971446486E-5 72-73 0.0 74-75 4.1630666814402163E-4 76-77 0.057914662256804854 78-79 0.14492275589890535 80-81 9.286841058597405E-4 82-83 0.0010087430805028215 84-85 0.0 86-87 0.10391654908671925 88-89 0.3592086074285762 90-91 0.3932016480620284 92-93 3.842830782867892E-4 94-95 2.4017692392924327E-4 96-97 9.60707695716973E-5 98-99 0.0 100-101 0.0011048138500745189 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3122698.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.4051129531023 #Duplication Level Percentage of deduplicated Percentage of total 1 71.35419986657345 33.82553904353151 2 15.238920418239221 14.448054874199343 3 5.544316956695649 7.884869147398728 4 2.6175776352999844 4.963462538596406 5 1.4106605942792043 3.343626240514805 6 0.8672561198823838 2.4667424593376177 7 0.545559517209804 1.8103617375179502 8 0.3931537827452058 1.4909999583180737 9 0.2846343735314766 1.2143812164835641 >10 1.5241219127600543 13.409360845895257 >50 0.1383146712263575 4.559837339924868 >100 0.07485627926796515 6.358594439389603 >500 0.004601003323147151 1.431033225481387 >1k 0.0016238835254235595 1.4327332086402051 >5k 6.766181355931497E-5 0.3193723595959193 >10k+ 1.3532362711862994E-4 1.0410313651747671 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17203 0.5509018163139695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15253 0.4884558160923663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9957 0.3188588842084633 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4673 0.14964623540284716 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4448 0.14244092768496985 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3846 0.12316272659091594 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.202358985723243E-5 0.0 5 0.0 0.0 0.0 3.202358985723243E-5 0.0 6 0.0 0.0 0.0 3.202358985723243E-5 0.0 7 0.0 0.0 0.0 3.202358985723243E-5 0.0 8 0.0 0.0 0.0 1.2809435942892972E-4 0.0 9 0.0 0.0 0.0 3.5225948842955673E-4 0.0 10-11 0.0 0.0 0.0 3.842830782867892E-4 0.0 12-13 0.0 0.0 0.0 4.1630666814402163E-4 0.0 14-15 0.0 0.0 0.0 4.963656427871026E-4 0.0 16-17 0.0 0.0 0.0 5.604128225015675E-4 0.0 18-19 0.0 0.0 0.0 5.764246174301838E-4 0.0 20-21 0.0 0.0 0.0 6.404717971446486E-4 0.0 22-23 0.0 0.0 0.0 8.966605160025082E-4 0.0 24-25 0.0 0.0 0.0 0.0013930261587896107 0.0 26-27 0.0 0.0 0.0 0.0022576630849348864 0.0 28-29 0.0 0.0 0.0 0.004451278990155308 0.0 30-31 0.0 0.0 0.0 0.01061582003767255 3.202358985723243E-5 32-33 0.0 0.0 0.0 0.02377751546899508 3.202358985723243E-5 34-35 0.0 0.0 0.0 0.04497713195448295 3.202358985723243E-5 36-37 0.0 0.0 0.0 0.08194836644465779 3.202358985723243E-5 38-39 0.0 0.0 0.0 0.13722108253824097 3.202358985723243E-5 40-41 0.0 0.0 0.0 0.19684900685240775 3.202358985723243E-5 42-43 0.0 0.0 0.0 0.256540978346289 3.202358985723243E-5 44-45 0.0 0.0 0.0 0.33312539348986037 3.202358985723243E-5 46-47 0.0 0.0 0.0 0.4309414487087768 3.202358985723243E-5 48-49 0.0 0.0 0.0 0.5378842270370046 3.202358985723243E-5 50-51 0.0 0.0 0.0 0.6511996997468215 3.202358985723243E-5 52-53 0.0 0.0 0.0 0.7650275498943542 3.202358985723243E-5 54-55 0.0 0.0 0.0 0.8943388057378587 3.202358985723243E-5 56-57 0.0 0.0 0.0 1.0510142191143683 3.202358985723243E-5 58-59 0.0 0.0 0.0 1.2284409187183647 3.202358985723243E-5 60-61 0.0 0.0 0.0 1.4259143855729886 3.202358985723243E-5 62-63 0.0 0.0 0.0 1.6197691867737452 3.202358985723243E-5 64-65 0.0 0.0 0.0 1.8248962916042473 3.202358985723243E-5 66-67 0.0 0.0 0.0 2.0652173216878484 3.202358985723243E-5 68-69 0.0 0.0 0.0 2.3362809980343924 3.202358985723243E-5 70-71 0.0 0.0 0.0 2.6342765134508683 3.202358985723243E-5 72-73 0.0 0.0 0.0 2.9418951176194432 3.202358985723243E-5 74-75 0.0 0.0 0.0 3.2494176510184465 3.202358985723243E-5 76-77 0.0 0.0 0.0 3.579692945011013 3.202358985723243E-5 78-79 0.0 0.0 0.0 3.963271504320943 3.202358985723243E-5 80-81 0.0 0.0 0.0 4.382924637605045 3.202358985723243E-5 82-83 0.0 0.0 0.0 4.815098994523326 3.202358985723243E-5 84-85 0.0 0.0 0.0 5.24144505808759 3.202358985723243E-5 86-87 0.0 0.0 0.0 5.6850678483798305 3.202358985723243E-5 88-89 0.0 0.0 0.0 6.170833682924189 3.202358985723243E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18590 0.0 26.411016 1 GTATCAA 25285 0.0 25.532991 1 GAGTACT 15595 0.0 23.45649 12-13 TACCTGG 2095 0.0 22.88186 2 GTACATG 16160 0.0 22.109728 1 GTACTTT 16730 0.0 21.680813 14-15 ATCAACG 29755 0.0 21.566057 3 TATCAAC 29915 0.0 21.466576 2 TCAACGC 29960 0.0 21.450174 4 TACATGG 16430 0.0 21.23263 2 CAACGCA 30565 0.0 21.010065 5 AACGCAG 30865 0.0 20.813892 6 AGTACTT 16350 0.0 20.748287 12-13 ACATGGG 16345 0.0 20.384787 3 GTACCTG 2605 0.0 19.176369 1 ACGCAGA 33840 0.0 18.940794 7 ACTTTTT 19315 0.0 18.913834 16-17 CGCAGAG 34080 0.0 18.779564 8 GCAGAGT 34900 0.0 18.066444 9 TACTTTT 18750 0.0 18.054552 14-15 >>END_MODULE