FastQCFastQC Report
Thu 26 May 2016
SRR938679_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938679_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001603
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100780.3357539288173686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83190.2771519084968932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51320.1709753088599658No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37170.12383383145605864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141150.028.646641
GGTATCA99450.028.3607521
ATCAACG166650.024.1370033
TCAACGC166950.024.0368734
TATCAAC168850.023.8225142
CAACGCA170200.023.5778835
GTACATG103900.023.3593061
AACGCAG173200.023.1806096
TACATGG105750.022.4907682
ACATGGG104100.022.2100773
GAGTACT98350.021.49612412-13
ACGCAGA192200.020.76737
CGCAGAG193300.020.600138
CATGGGG62250.020.0169244
GTACTTT106350.019.74571414-15
GTGTAGC18500.019.7298851
TACCTGG22850.019.697832
GCAGAGT203650.019.0881829
AGAGTAC151650.018.98309310-11
AGTACTT105200.018.5886312-13
Produced by FastQC (version 0.11.5)