##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938679_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3001603 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.621821406761654 33.0 31.0 34.0 30.0 34.0 2 31.709915668394522 33.0 31.0 34.0 30.0 34.0 3 31.726932242538403 33.0 31.0 34.0 30.0 34.0 4 35.27240777677794 37.0 35.0 37.0 33.0 37.0 5 35.298806337813495 37.0 35.0 37.0 33.0 37.0 6 35.37323889934812 37.0 35.0 37.0 33.0 37.0 7 35.283801688631044 37.0 35.0 37.0 33.0 37.0 8 35.252469763656286 37.0 35.0 37.0 32.0 37.0 9 36.83884910829313 39.0 37.0 39.0 33.0 39.0 10-11 36.73240648413531 39.0 37.0 39.0 32.0 39.0 12-13 36.61549012311089 39.0 37.0 39.0 32.0 39.0 14-15 37.79524757271365 40.0 38.0 41.0 32.0 41.0 16-17 37.716356893300016 40.0 38.0 41.0 32.0 41.0 18-19 37.6359921681848 40.0 37.0 41.0 32.0 41.0 20-21 37.518643871291445 40.0 37.0 41.0 32.0 41.0 22-23 37.439390552314876 40.0 37.0 41.0 32.0 41.0 24-25 37.45192585428519 40.0 37.0 41.0 32.0 41.0 26-27 37.35924004606872 40.0 37.0 41.0 31.0 41.0 28-29 37.233407282708605 40.0 37.0 41.0 31.0 41.0 30-31 37.02873331349949 40.0 36.5 41.0 30.5 41.0 32-33 36.81727613545162 39.5 36.0 41.0 30.0 41.0 34-35 36.49723597690967 39.0 36.0 41.0 30.0 41.0 36-37 36.24735816162231 39.0 35.0 41.0 30.0 41.0 38-39 36.07284924088896 39.0 35.0 41.0 29.0 41.0 40-41 35.88525098089254 39.0 35.0 41.0 28.5 41.0 42-43 35.62559539019651 39.0 35.0 40.0 27.0 41.0 44-45 35.60299113507016 39.0 35.0 41.0 27.0 41.0 46-47 35.437056133006266 39.0 35.0 41.0 26.0 41.0 48-49 35.212770809464146 39.0 35.0 41.0 25.5 41.0 50-51 34.07775295400491 38.0 33.5 40.0 23.5 40.5 52-53 34.18112721769002 38.0 33.5 39.5 23.5 40.5 54-55 34.68919707236434 38.0 34.0 40.0 24.0 41.0 56-57 34.58211245790999 38.0 34.0 40.0 23.5 41.0 58-59 34.35453922454102 38.0 34.0 40.0 23.0 41.0 60-61 34.05935161978449 38.0 34.0 40.0 22.5 41.0 62-63 33.69533146122255 37.0 33.5 40.0 22.0 41.0 64-65 33.30809820619183 36.5 33.0 40.0 20.5 41.0 66-67 32.90695305141952 36.0 32.5 39.0 19.5 41.0 68-69 32.50675955481121 35.5 32.0 39.0 18.0 41.0 70-71 32.10173097508232 35.0 32.0 38.5 16.5 40.0 72-73 31.69103009292035 35.0 31.0 37.5 13.0 40.0 74-75 31.24683793959428 35.0 31.0 37.0 9.5 39.0 76-77 30.823399030451395 35.0 31.0 36.5 7.0 39.0 78-79 30.421050685250513 35.0 30.5 36.0 4.5 38.5 80-81 30.000199726612745 34.0 30.0 35.5 2.0 37.5 82-83 29.55489400163846 34.0 29.5 35.0 2.0 37.0 84-85 29.177114028737314 34.0 29.0 35.0 2.0 36.5 86-87 28.813569449390876 34.0 29.0 35.0 2.0 36.0 88-89 28.459824133971082 34.0 29.0 35.0 2.0 36.0 90-91 28.073566857442508 34.0 28.5 35.0 2.0 35.0 92-93 27.649004048836574 34.0 27.0 35.0 2.0 35.0 94-95 27.168171140553895 34.0 26.0 35.0 2.0 35.0 96-97 26.608629955393834 33.0 25.0 35.0 2.0 35.0 98-99 25.932358476454084 33.0 24.0 35.0 2.0 35.0 100-101 23.731569597978147 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20990037992589805 1101 2 0.060464047748030936 1101 3 0.16855747493226048 1101 4 0.14529559227329258 1101 5 -0.03378632661409853 1101 6 -0.1113316187793032 1101 7 0.060506187854052484 1101 8 0.1714351089254862 1101 9 0.20798930405425864 1101 10-11 0.2266684136477508 1101 12-13 0.18658716570147504 1101 14-15 0.29111898237944445 1101 16-17 0.17711362450543078 1101 18-19 0.2669987095598927 1101 20-21 0.3696822469288463 1101 22-23 0.2651983645931608 1101 24-25 0.189937704047054 1101 26-27 0.23240129494347173 1101 28-29 0.17862002330225124 1101 30-31 0.18795716369307058 1101 32-33 0.14379558646488988 1101 34-35 0.07621127143342932 1101 36-37 0.21400865166378225 1101 38-39 0.09847799766946963 1101 40-41 0.09834327231567386 1101 42-43 0.10367750136835951 1101 44-45 0.2239847110744151 1101 46-47 0.21641840560821635 1101 48-49 0.18717229032385774 1101 50-51 0.05369762546921919 1101 52-53 0.04575034397324629 1101 54-55 0.06187832294013873 1101 56-57 0.06407227622931799 1101 58-59 -0.1576905591067259 1101 60-61 -0.1989403460263972 1101 62-63 -0.18198151027934273 1101 64-65 -0.25377037429183247 1101 66-67 -0.33570086159627266 1101 68-69 -0.3542156843028508 1101 70-71 -0.4021150125921622 1101 72-73 -0.5006900373023342 1101 74-75 -0.45390029094187767 1101 76-77 -0.46116011415971414 1101 78-79 -0.46661878141037505 1101 80-81 -0.5452383523786999 1101 82-83 -0.6718967536617733 1101 84-85 -0.5856297705141458 1101 86-87 -0.7019610766908677 1101 88-89 -0.712635612519481 1101 90-91 -0.8112525792194454 1101 92-93 -0.9204489937461631 1101 94-95 -0.7983428919462732 1101 96-97 -0.9533415307631259 1101 98-99 -1.2406862530129992 1101 100-101 -1.0828146639843759 1102 1 0.14742326187715804 1102 2 0.05508189504583427 1102 3 0.12718770146499736 1102 4 0.14738830488960275 1102 5 0.06930609887840689 1102 6 0.0512779393791547 1102 7 0.11717443956516149 1102 8 0.1260166628667463 1102 9 0.22209990686670977 1102 10-11 0.09741361907745016 1102 12-13 0.10032859312668307 1102 14-15 0.004052037138379205 1102 16-17 0.09017106174957945 1102 18-19 0.2083243363239191 1102 20-21 0.1950373214732224 1102 22-23 0.20326842734048967 1102 24-25 0.2432405924217136 1102 26-27 0.2927088692239934 1102 28-29 0.11311272774744197 1102 30-31 0.2314529016636655 1102 32-33 0.19370740654607488 1102 34-35 0.15483881965717217 1102 36-37 0.1472528782623428 1102 38-39 0.08689794290032182 1102 40-41 0.10802484861399364 1102 42-43 0.10208731215036693 1102 44-45 0.25702295755375104 1102 46-47 -0.008960728321987688 1102 48-49 -0.12716835199364596 1102 50-51 -0.23637186303051294 1102 52-53 -0.19995397752370536 1102 54-55 -0.23618648532811903 1102 56-57 -0.18569002384796107 1102 58-59 -0.1343414358557098 1102 60-61 -0.19518452836476285 1102 62-63 -0.23938345977051512 1102 64-65 -0.23458579454234751 1102 66-67 -0.25672683551908193 1102 68-69 -0.2677293987202134 1102 70-71 -0.30921531185914475 1102 72-73 -0.2819939084802101 1102 74-75 -0.3742569713367665 1102 76-77 -0.36575086060215867 1102 78-79 -0.527778250878221 1102 80-81 -0.5344402276489397 1102 82-83 -0.6538865443110673 1102 84-85 -0.6191596455755679 1102 86-87 -0.6988210431212174 1102 88-89 -0.7292844237928868 1102 90-91 -0.6212098170657647 1102 92-93 -0.7012705423308034 1102 94-95 -0.6785055878685498 1102 96-97 -0.5255619624021257 1102 98-99 -0.5114356574864587 1102 100-101 -0.42522545983935345 1103 1 0.0668095714096637 1103 2 0.16826119723215882 1103 3 0.046959575689122346 1103 4 0.19656240779448098 1103 5 0.11753965761130303 1103 6 0.07122360980896758 1103 7 -0.0013545727174104627 1103 8 0.04432076241253924 1103 9 0.1649048899982546 1103 10-11 0.137288164505442 1103 12-13 0.2208467687046678 1103 14-15 0.2089314751486313 1103 16-17 0.25817503423428434 1103 18-19 0.3756097564287799 1103 20-21 0.2652173977098755 1103 22-23 0.29462462193382066 1103 24-25 0.29066943090808195 1103 26-27 0.20438096397943895 1103 28-29 0.33684467265843665 1103 30-31 0.3054944667057953 1103 32-33 0.3113170515052559 1103 34-35 0.29370344847813357 1103 36-37 0.2945174136130717 1103 38-39 0.11355302315327975 1103 40-41 0.25046252295788207 1103 42-43 0.3334027595660629 1103 44-45 0.298029369027212 1103 46-47 0.22483168014291266 1103 48-49 0.28830535220228626 1103 50-51 0.27762968507015273 1103 52-53 0.25690659118421877 1103 54-55 0.41067715037166863 1103 56-57 0.3507064067576877 1103 58-59 0.25278523090831584 1103 60-61 0.35196462967294906 1103 62-63 0.33014670628519127 1103 64-65 0.12386011686540144 1103 66-67 0.15983053913588918 1103 68-69 0.13981467103194944 1103 70-71 0.05134682393556744 1103 72-73 0.20087110006355147 1103 74-75 0.17830323663448766 1103 76-77 0.12183439446932809 1103 78-79 0.09385645578591095 1103 80-81 0.016112561122948676 1103 82-83 -0.03760738963244492 1103 84-85 -0.046184955560821805 1103 86-87 -0.04593807076795642 1103 88-89 -0.09002035579329615 1103 90-91 -0.18198372134640906 1103 92-93 -0.16387879984128162 1103 94-95 -0.21135891263377715 1103 96-97 -0.1696485099916174 1103 98-99 -0.23521579798389425 1103 100-101 0.012866579126047384 1104 1 0.001352468111235794 1104 2 0.044883912480116805 1104 3 0.04944909934189923 1104 4 0.029191996803298537 1104 5 0.12643673961302682 1104 6 0.1194932756405862 1104 7 0.10438683429458706 1104 8 0.1189005730895758 1104 9 0.020664958600960404 1104 10-11 0.06382833031928925 1104 12-13 0.10398592031727816 1104 14-15 0.16640277789404223 1104 16-17 0.15397230910242143 1104 18-19 0.04908526185436557 1104 20-21 0.05696843500202675 1104 22-23 0.07836972439045553 1104 24-25 0.08190013583505618 1104 26-27 0.1075733438448836 1104 28-29 0.17743669026418019 1104 30-31 0.09591957549387331 1104 32-33 0.1579038802894388 1104 34-35 0.18037172049128003 1104 36-37 0.1530275936232286 1104 38-39 0.21851941205452619 1104 40-41 0.04240529123174497 1104 42-43 0.07059601810014016 1104 44-45 0.24621439442721993 1104 46-47 0.20467586935075843 1104 48-49 0.24503148414018483 1104 50-51 0.3150483104169055 1104 52-53 0.34169075077634403 1104 54-55 0.2823472930406581 1104 56-57 0.19742459740180607 1104 58-59 0.1055785562643834 1104 60-61 0.07210838912459394 1104 62-63 0.1144802671550238 1104 64-65 0.04774061253524309 1104 66-67 0.14830824348143068 1104 68-69 0.08298158344702955 1104 70-71 0.09055780513502754 1104 72-73 0.10497649263371756 1104 74-75 -0.08269473577243858 1104 76-77 -0.10592739729519707 1104 78-79 0.0643263996077792 1104 80-81 -0.052898900691165096 1104 82-83 -0.17908152714094072 1104 84-85 -0.23327015673951124 1104 86-87 -0.18738387863271555 1104 88-89 -0.3738967639002517 1104 90-91 -0.4038879095919441 1104 92-93 -0.3507626019852488 1104 94-95 -0.3032123301106502 1104 96-97 -0.2037462715450502 1104 98-99 -0.24077318597275266 1104 100-101 -0.05524811456080414 1105 1 0.19348841336184464 1105 2 0.12787519625248223 1105 3 0.15676882580956075 1105 4 0.2844427929068871 1105 5 0.12695048463989878 1105 6 0.1111794592527886 1105 7 0.1988106216282901 1105 8 0.18698050805991784 1105 9 0.27346104609053157 1105 10-11 0.1853246697183053 1105 12-13 0.21493908501346226 1105 14-15 0.3528469513815864 1105 16-17 0.2587494671668864 1105 18-19 0.27365924247960294 1105 20-21 0.34603921377510005 1105 22-23 0.47076831004905273 1105 24-25 0.38708513769956454 1105 26-27 0.312371494362786 1105 28-29 0.3544620931449103 1105 30-31 0.31159557273601735 1105 32-33 0.4385176513388984 1105 34-35 0.31479719795222394 1105 36-37 0.31391630545116556 1105 38-39 0.45210505724048033 1105 40-41 0.3862955483188699 1105 42-43 0.3013832283975759 1105 44-45 0.35157605482968535 1105 46-47 0.34501645681752535 1105 48-49 0.325481540880169 1105 50-51 0.3126304895006413 1105 52-53 0.337163959576813 1105 54-55 0.3061455589164481 1105 56-57 0.21110590915516525 1105 58-59 0.20525045696001598 1105 60-61 0.3887024205609606 1105 62-63 0.3450555408617433 1105 64-65 0.20556697476438757 1105 66-67 0.18599539824611355 1105 68-69 0.170864846626678 1105 70-71 0.20129098501553955 1105 72-73 0.2968186776731443 1105 74-75 0.28109509288085377 1105 76-77 0.20214993541573634 1105 78-79 0.13758000185913488 1105 80-81 0.01743319589826342 1105 82-83 0.021318939119559843 1105 84-85 0.07470288438959827 1105 86-87 0.13172388173406802 1105 88-89 0.15321502326175462 1105 90-91 0.16145026340733537 1105 92-93 0.07523732289797991 1105 94-95 0.3412831534937766 1105 96-97 0.386204714764915 1105 98-99 0.3479568175416361 1105 100-101 0.5473915690903013 1106 1 0.2293136045046964 1106 2 0.1958527930206806 1106 3 0.2572842315513455 1106 4 0.34291012346406546 1106 5 0.21729980154067619 1106 6 0.24646486222110298 1106 7 0.22771151474942286 1106 8 0.25728750993027916 1106 9 0.2836777547392302 1106 10-11 0.373868479765072 1106 12-13 0.26400007081913657 1106 14-15 0.4243605037455964 1106 16-17 0.3301573660470538 1106 18-19 0.4235624837787455 1106 20-21 0.4393414645351257 1106 22-23 0.33580484011822165 1106 24-25 0.28964653349215297 1106 26-27 0.06793721485249904 1106 28-29 0.2759932898083335 1106 30-31 0.34244761919539 1106 32-33 0.40097054865800885 1106 34-35 0.35411822877264854 1106 36-37 0.39963742986795125 1106 38-39 0.23983955377900656 1106 40-41 0.20369710731955593 1106 42-43 0.42768182760335094 1106 44-45 0.32254721539548825 1106 46-47 0.5110871395010577 1106 48-49 0.5516762633238272 1106 50-51 0.5944483909442084 1106 52-53 0.5036148495356016 1106 54-55 0.34057479432492244 1106 56-57 0.25438131912532214 1106 58-59 0.2758929128319494 1106 60-61 0.2776611118925416 1106 62-63 0.14332728229263125 1106 64-65 0.24217755513109296 1106 66-67 0.19078818891275517 1106 68-69 0.41250218621048873 1106 70-71 0.27651939432663397 1106 72-73 0.19571896514448284 1106 74-75 0.1375677124816015 1106 76-77 0.03051085039837531 1106 78-79 0.20820513748781622 1106 80-81 0.08781562463440551 1106 82-83 0.16289455159129673 1106 84-85 0.3000504234512178 1106 86-87 0.45443660952041043 1106 88-89 0.509156246625178 1106 90-91 0.5602652347127197 1106 92-93 0.5439314860654001 1106 94-95 0.522377024072 1106 96-97 0.38679756992991443 1106 98-99 0.4383367521627477 1106 100-101 0.6942948325146645 1107 1 0.01144995942861371 1107 2 -0.0017905493632319747 1107 3 0.06115005446421051 1107 4 0.05416102028800651 1107 5 0.05773125503083065 1107 6 -0.011780730878278689 1107 7 -0.09376514337809283 1107 8 0.032010606249365026 1107 9 -0.024834953664040427 1107 10-11 -0.04815626018261554 1107 12-13 0.07166561215424139 1107 14-15 0.07197494924790959 1107 16-17 0.041808153541758486 1107 18-19 0.19623173129453164 1107 20-21 0.048398228373748964 1107 22-23 0.05377441612644418 1107 24-25 -0.05965866838394618 1107 26-27 0.0485260584915963 1107 28-29 -0.09223423839402045 1107 30-31 -0.02672930587358735 1107 32-33 0.06148036075593666 1107 34-35 0.02913418570580717 1107 36-37 0.1362377838084896 1107 38-39 0.12461075698504231 1107 40-41 0.10928219655108506 1107 42-43 0.17329550753177614 1107 44-45 0.18728244358215562 1107 46-47 0.3244155835994107 1107 48-49 0.4115396219546028 1107 50-51 0.48949209067818344 1107 52-53 0.3891410840122944 1107 54-55 0.35200535315012615 1107 56-57 0.261716180989481 1107 58-59 0.2789449461731124 1107 60-61 0.09440626315507927 1107 62-63 0.15198658923628727 1107 64-65 0.13327192091772844 1107 66-67 0.22478736367209962 1107 68-69 0.173432334938866 1107 70-71 0.15820917943320723 1107 72-73 0.05413966672914228 1107 74-75 0.13090825143620677 1107 76-77 0.03367380559435773 1107 78-79 0.06641565954169337 1107 80-81 0.14456050896668415 1107 82-83 0.16743791749423664 1107 84-85 0.09778043965657801 1107 86-87 0.18740418859877295 1107 88-89 0.17212159268622074 1107 90-91 0.2224048354187147 1107 92-93 0.37964197531377764 1107 94-95 0.26503007744560136 1107 96-97 0.3981049819435931 1107 98-99 0.41836257486027506 1107 100-101 0.5131664533095162 1108 1 0.02864417461981006 1108 2 0.041529223884467115 1108 3 1.4075485283271405E-4 1108 4 -0.0017850861316119904 1108 5 0.09311296833745075 1108 6 0.024470326701973022 1108 7 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2316 80-81 -1.381572182840845 2316 82-83 -1.2989002929157891 2316 84-85 -1.2909989848048546 2316 86-87 -1.398186492134105 2316 88-89 -1.527853438018976 2316 90-91 -1.71717151782995 2316 92-93 -1.9183676076895537 2316 94-95 -1.91328264686085 2316 96-97 -1.922618630108925 2316 98-99 -2.032545681926095 2316 100-101 -2.234779431228013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13122.0 3 3996.0 4 3856.0 5 4207.0 6 4633.0 7 5340.0 8 6407.0 9 7868.0 10 9120.0 11 11615.0 12 14070.0 13 17590.0 14 19313.0 15 18045.0 16 21281.0 17 25718.0 18 22698.0 19 18124.0 20 18564.0 21 19736.0 22 21353.0 23 23980.0 24 27421.0 25 31566.0 26 36214.0 27 42432.0 28 50377.0 29 60167.0 30 72401.0 31 88197.0 32 110537.0 33 141953.0 34 190123.0 35 269598.0 36 398354.0 37 576806.0 38 508582.0 39 86196.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.818231686930545 18.483108538724935 14.258591545160126 23.440068229184394 2 15.78523877549115 19.819306115208192 39.802139729312636 24.593315379988024 3 19.72776163755146 23.68289695814993 29.416454405926117 27.17288699837249 4 13.098935414904059 16.169201099300913 36.67231034941588 34.059553136379144 5 14.096142813304693 36.725393924030364 34.19510747021388 14.983355792451066 6 32.6557151950835 35.27816937787242 17.326354519731645 14.739760907312425 7 28.805601095582507 32.101614011137805 20.540938864083046 18.551846029196646 8 26.415266648401936 34.45458114773167 19.724909159629078 19.40524304423731 9 26.219461590072125 16.839033969840806 20.279713986599248 36.661790453487825 10-11 25.300896619682305 26.045732719910795 27.148380116884024 21.504990543522876 12-13 26.133195298597332 23.436118326344378 27.51363154124827 22.91705483381002 14-15 22.96891883874606 25.040512966482392 25.891835212383935 26.098732982387606 16-17 22.52520106608646 28.10549009863783 26.627230866579378 22.742077968696332 18-19 22.35053957227192 26.74546251158881 28.7957754823386 22.108222433800677 20-21 23.065527403594484 25.87557557989638 28.91611403719143 22.142782979317705 22-23 22.701163842137504 25.831828353077952 28.836966259828905 22.630041544955642 24-25 22.644239328960293 26.03277913476741 28.93320276892567 22.38977876734663 26-27 22.438971612637136 26.177852752339387 28.87685361572492 22.50632201929856 28-29 22.27600508082987 26.51754786615147 28.83917706558738 22.36726998743128 30-31 22.49180878714047 26.175179449981183 28.678628776466642 22.654382986411704 32-33 22.25475556385138 26.25576217347684 28.7840522370399 22.70543002563188 34-35 22.860283095415777 26.297829715412746 28.50607018463565 22.335817004535826 36-37 22.83687793720672 26.07636289822302 28.554447903451695 22.532311261118565 38-39 22.474108792177518 26.359751642496633 28.61453407704731 22.551605488278543 40-41 22.7385677654391 26.246660946158645 28.643353087485302 22.371418200916953 42-43 22.562558372142128 26.342663841045066 28.27649463637188 22.818283150440926 44-45 22.617309756926872 26.418757647071423 28.302363530731522 22.661569065270182 46-47 22.919051137785953 26.56576923540302 27.849468087242528 22.665711539568502 48-49 22.8125053868048 26.66017767627759 27.761621314860683 22.76569562205692 50-51 22.58399357148427 26.84141265361351 27.872508690778957 22.70208508412326 52-53 22.884161476546726 26.76510378454799 27.67686683660628 22.673867902299005 54-55 22.62691959890147 26.680508729267867 27.75298410907117 22.9395875627595 56-57 22.683270656250638 26.971601074617237 27.568368991624503 22.776759277507622 58-59 22.88670181616914 26.63952256625156 27.64489802813867 22.82887758944063 60-61 22.628673267671097 26.641430482942162 28.064719808464524 22.665176440922213 62-63 22.531469196025537 26.714919768524314 28.076415486411477 22.677195549038675 64-65 22.40802043034545 26.774625157457134 27.900232348649507 22.917122063547907 66-67 22.439104242182406 26.866173278575918 27.641471915844612 23.053250563397068 68-69 22.525926801333927 26.654378839806398 27.808157907335435 23.01153645152424 70-71 22.544963027749066 26.595180006946684 27.694864895402215 23.164992069902034 72-73 22.57039760013564 26.549060190929342 27.78372231709495 23.09681989184007 74-75 22.448734697730277 26.99898947196479 27.49821174361441 23.054064086690516 76-77 22.560942653557447 26.697551651556328 27.532436847730253 23.20906884715597 78-79 22.580263632249824 26.783658112852134 27.527496196244506 23.108582058653536 80-81 22.691253457214195 26.84218574232487 27.3830101469613 23.08355065349964 82-83 22.662712389047375 26.781474571875545 27.521303740389687 23.034509298687393 84-85 22.57669924939591 26.782354261972905 27.49785587103169 23.143090617599494 86-87 22.661675063071343 26.720830686013063 27.488412727283983 23.129081523631612 88-89 22.62009077095062 26.70041569882252 27.27283833265278 23.406655197574086 90-91 22.597722840745462 26.700907288904542 27.321091103106127 23.38027876724388 92-93 22.780549582103845 26.558878429281503 27.415858849023667 23.24471313959099 94-95 22.5859183585003 26.4963733690119 27.50041884043878 23.417289432049017 96-97 22.594731350562988 26.260348152504765 27.62403974868856 23.520880748243687 98-99 22.574446890880402 26.327485317479177 27.33753831714513 23.760529474495293 100-101 22.82811302224377 25.89473656027247 27.644793162426346 23.63235725505742 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2368.0 1 1418.5 2 688.0 3 924.5 4 769.0 5 699.5 6 1275.5 7 2573.0 8 4030.5 9 4644.0 10 3967.5 11 3128.0 12 3068.5 13 3317.0 14 3606.0 15 3839.0 16 4141.5 17 4515.5 18 4763.5 19 5027.0 20 5433.5 21 6215.5 22 7281.0 23 8572.5 24 10412.0 25 12886.5 26 16290.0 27 20510.5 28 24229.5 29 27968.5 30 32853.0 31 38796.0 32 45222.0 33 49145.0 34 52353.0 35 57011.5 36 62733.5 37 68881.0 38 73752.5 39 77207.5 40 80063.0 41 84619.5 42 88713.0 43 92979.0 44 97932.0 45 102072.5 46 105079.0 47 109207.0 48 118311.0 49 119817.0 50 113883.0 51 110766.0 52 108265.5 53 107394.0 54 106214.5 55 100572.5 56 92575.5 57 84058.0 58 77089.5 59 69590.0 60 58587.0 61 48268.5 62 39954.0 63 31431.0 64 23437.5 65 18006.0 66 14029.5 67 11002.5 68 8252.5 69 5900.0 70 4388.0 71 3220.0 72 2487.0 73 2039.5 74 1627.5 75 1293.5 76 936.0 77 619.5 78 419.0 79 272.0 80 195.0 81 157.5 82 120.0 83 88.5 84 71.5 85 56.0 86 45.5 87 29.5 88 22.0 89 23.5 90 21.0 91 16.5 92 13.0 93 9.0 94 8.0 95 6.5 96 4.0 97 5.0 98 3.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15475064490540555 2 0.09058493078531706 3 0.08462145060489346 4 0.09894712925060377 5 0.11870323956899032 6 0.03641387618549156 7 0.039645482763709924 8 0.042810458278459876 9 0.044109764016094066 10-11 0.042593907322187506 12-13 0.04637522017402035 14-15 0.044276341674765116 16-17 0.04276048498085856 18-19 0.046725033257229556 20-21 0.0491903826055611 22-23 0.06131723615681354 24-25 0.07719208702816462 26-27 0.07874125925380539 28-29 0.06972940791970157 30-31 0.05803565628099386 32-33 0.04774115697512296 34-35 0.2883292693937206 36-37 0.4581385346429891 38-39 0.43605033710320784 40-41 0.4117133411713675 42-43 0.4408977469705354 44-45 0.4507924598955958 46-47 0.45039267351478524 48-49 0.46570116034665476 50-51 0.4552900566797142 52-53 0.462253002812164 54-55 0.4533910713708642 56-57 0.4332351746716671 58-59 0.41216310084977925 60-61 0.3815294694201732 62-63 0.43726635401150654 64-65 0.4758124242279875 66-67 0.4786442444253954 68-69 0.4876061224618979 70-71 0.48525737747463604 72-73 0.47532934901784146 74-75 0.46828311405605605 76-77 0.45067585553452605 78-79 0.4257891533290712 80-81 0.4306032476646645 82-83 0.4423636303668406 84-85 0.44009817420891434 86-87 0.4503760157489181 88-89 0.46185321643135346 90-91 0.4671170704453587 92-93 0.4690327135200758 94-95 0.4726974220108389 96-97 0.47421327870474544 98-99 0.46765011895310604 100-101 0.4545071416839602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3001603.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.027385561999246 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8387227448346 45.16777576368078 2 12.902242115901519 14.973667557473647 3 3.9588664420537203 6.891680082645342 4 1.7298092834563734 4.015052409593946 5 0.9426315073861938 2.734922096099361 6 0.5606281066409712 1.9519069980569559 7 0.3732785890293366 1.5162266425351063 8 0.2663425808955253 1.2364130906562099 9 0.19880061076188463 1.0382291721576735 >10 1.1194592166238624 11.772741201822353 >50 0.07379267463498335 2.9072178699720093 >100 0.03220200857473909 3.350273640383988 >500 0.002245368733096766 0.9092104238438132 >1k 8.060298014999323E-4 0.7486161004979504 >5k 1.1514711449999034E-4 0.4493767925650629 >10k+ 5.757355724999517E-5 0.33669015801581326 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10078 0.3357539288173686 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8319 0.2771519084968932 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5132 0.1709753088599658 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3717 0.12383383145605864 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.663106346842005E-5 2 0.0 0.0 0.0 0.0 6.663106346842005E-5 3 0.0 0.0 0.0 0.0 6.663106346842005E-5 4 0.0 0.0 0.0 0.0 6.663106346842005E-5 5 0.0 0.0 0.0 0.0 6.663106346842005E-5 6 0.0 0.0 0.0 3.3315531734210023E-5 6.663106346842005E-5 7 0.0 0.0 0.0 3.3315531734210023E-5 6.663106346842005E-5 8 0.0 0.0 0.0 3.3315531734210023E-5 6.663106346842005E-5 9 0.0 0.0 0.0 3.3315531734210023E-5 6.663106346842005E-5 10-11 0.0 0.0 0.0 3.3315531734210023E-5 8.328882933552505E-5 12-13 0.0 0.0 0.0 6.663106346842005E-5 2.3320872213947015E-4 14-15 0.0 1.6657765867105012E-5 0.0 8.328882933552505E-5 3.1649755147499523E-4 16-17 0.0 3.3315531734210023E-5 0.0 1.665776586710501E-4 3.664708490763102E-4 18-19 0.0 3.3315531734210023E-5 0.0 2.3320872213947015E-4 3.997863808105202E-4 20-21 0.0 3.3315531734210023E-5 0.0 2.4986648800657516E-4 5.330485077473604E-4 22-23 0.0 3.3315531734210023E-5 0.0 4.997329760131503E-4 5.663640394815703E-4 24-25 0.0 3.3315531734210023E-5 0.0 7.329416981526204E-4 5.663640394815703E-4 26-27 0.0 3.3315531734210023E-5 0.0 0.0010827547813618255 5.996795712157804E-4 28-29 0.0 3.3315531734210023E-5 0.0 0.002398718284863121 5.996795712157804E-4 30-31 0.0 3.3315531734210023E-5 0.0 0.007279443683924889 5.996795712157804E-4 32-33 0.0 3.3315531734210023E-5 0.0 0.017207472140719476 5.996795712157804E-4 34-35 0.0 3.3315531734210023E-5 0.0 0.034131762261698166 6.329951029499903E-4 36-37 0.0 3.3315531734210023E-5 0.0 0.06208349338670037 6.329951029499903E-4 38-39 0.0 3.3315531734210023E-5 0.0 0.10569352442678129 6.663106346842004E-4 40-41 0.0 3.3315531734210023E-5 0.0 0.15210206013253585 6.663106346842004E-4 42-43 0.0 3.3315531734210023E-5 0.0 0.20029297678607064 6.663106346842004E-4 44-45 0.0 3.3315531734210023E-5 0.0 0.26349254048586707 6.829684005513054E-4 46-47 0.0 3.3315531734210023E-5 0.0 0.340884520704437 7.162839322855155E-4 48-49 0.0 4.9973297601315035E-5 0.0 0.428770893419283 7.329416981526204E-4 50-51 0.0 6.663106346842005E-5 0.0 0.5223708798265461 7.662572298868305E-4 52-53 0.0 6.663106346842005E-5 0.0 0.6127559174214579 8.495460592223556E-4 54-55 0.0 6.663106346842005E-5 0.0 0.7186160195069101 8.662038250894606E-4 56-57 0.0 6.663106346842005E-5 0.0 0.8511285469797305 8.995193568236705E-4 58-59 0.0 6.663106346842005E-5 0.0 1.000631995636998 9.328348885578806E-4 60-61 0.0 6.663106346842005E-5 0.0 1.1593805043505088 9.328348885578806E-4 62-63 0.0 6.663106346842005E-5 0.0 1.3239425733516392 9.994659520263006E-4 64-65 0.0 6.663106346842005E-5 0.0 1.4901537611736129 9.994659520263006E-4 66-67 0.0 9.994659520263006E-5 0.0 1.6929454028397493 9.994659520263006E-4 68-69 0.0 9.994659520263006E-5 0.0 1.9234555669087485 9.994659520263006E-4 70-71 0.0 9.994659520263006E-5 0.0 2.1759872974540606 0.0010827547813618257 72-73 0.0 9.994659520263006E-5 0.0 2.4223889701602777 0.0011327280789631407 74-75 0.0 9.994659520263006E-5 0.0 2.682533299706857 0.0011660436106973506 76-77 0.0 9.994659520263006E-5 0.0 2.968280615391176 0.0011660436106973506 78-79 1.6657765867105012E-5 9.994659520263006E-5 0.0 3.299970049336971 0.0011827013765644558 80-81 3.3315531734210023E-5 9.994659520263006E-5 0.0 3.6582785931384 0.0011993591424315608 82-83 3.3315531734210023E-5 9.994659520263006E-5 0.0 4.019768770220446 0.0013659368011026108 84-85 3.3315531734210023E-5 9.994659520263006E-5 0.0 4.393768929468687 0.0013659368011026108 86-87 3.3315531734210023E-5 9.994659520263006E-5 0.0 4.781311852366885 0.001382594566969716 88-89 3.3315531734210023E-5 9.994659520263006E-5 0.0 5.204602340815891 0.001399252332836821 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14115 0.0 28.64664 1 GGTATCA 9945 0.0 28.360752 1 ATCAACG 16665 0.0 24.137003 3 TCAACGC 16695 0.0 24.036873 4 TATCAAC 16885 0.0 23.822514 2 CAACGCA 17020 0.0 23.577883 5 GTACATG 10390 0.0 23.359306 1 AACGCAG 17320 0.0 23.180609 6 TACATGG 10575 0.0 22.490768 2 ACATGGG 10410 0.0 22.210077 3 GAGTACT 9835 0.0 21.496124 12-13 ACGCAGA 19220 0.0 20.7673 7 CGCAGAG 19330 0.0 20.60013 8 CATGGGG 6225 0.0 20.016924 4 GTACTTT 10635 0.0 19.745714 14-15 GTGTAGC 1850 0.0 19.729885 1 TACCTGG 2285 0.0 19.69783 2 GCAGAGT 20365 0.0 19.088182 9 AGAGTAC 15165 0.0 18.983093 10-11 AGTACTT 10520 0.0 18.58863 12-13 >>END_MODULE