FastQCFastQC Report
Thu 26 May 2016
SRR938679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001603
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96150.3203288376244293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88410.2945426160621508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56220.18729991940972873No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41520.13832608776044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111200.029.5957491
GTATCAA153300.025.8796831
TCAACGC184000.021.4572664
ATCAACG184450.021.4306433
GTACATG106200.021.391931
TATCAAC185900.021.3911192
CAACGCA187600.021.07085
TACATGG106500.020.9865062
AACGCAG191550.020.7353886
GAGTACT108000.020.476712-13
ACATGGG103700.020.4091513
GTACTTT113900.019.35351414-15
AGAGTAC165050.019.01975310-11
AGTACTT113700.018.80321512-13
ACGCAGA211950.018.627687
CGCAGAG212450.018.4498218
CATGGGG66400.017.9380194
GTGTAGC19150.017.4094621
GCAGAGT228350.016.8950089
TACCTGG23650.016.6538942
Produced by FastQC (version 0.11.5)