##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938679_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3001603 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91214561019562 31.0 31.0 34.0 30.0 34.0 2 32.1495770759824 33.0 31.0 34.0 30.0 34.0 3 32.23363649356694 34.0 31.0 34.0 30.0 34.0 4 35.88307947453411 37.0 35.0 37.0 35.0 37.0 5 35.78733063632999 37.0 35.0 37.0 35.0 37.0 6 35.81514677324083 37.0 35.0 37.0 33.0 37.0 7 35.74910805992665 37.0 35.0 37.0 33.0 37.0 8 35.71150548556888 37.0 35.0 37.0 33.0 37.0 9 37.35256361350918 39.0 37.0 39.0 34.0 39.0 10-11 37.26980166930804 39.0 37.0 39.0 33.5 39.0 12-13 37.195176044267015 39.0 37.0 39.0 33.0 39.0 14-15 38.392701333254266 40.0 38.0 41.0 33.0 41.0 16-17 38.30786732955691 40.0 38.0 41.0 33.0 41.0 18-19 38.31426141298499 40.0 38.0 41.0 33.5 41.0 20-21 38.39635787943975 40.0 38.0 41.0 34.0 41.0 22-23 38.3521778196517 40.0 38.0 41.0 34.0 41.0 24-25 38.28041050065582 40.0 38.0 41.0 33.0 41.0 26-27 38.108720740217805 40.0 37.5 41.0 33.0 41.0 28-29 37.93455496946132 40.0 37.0 41.0 33.0 41.0 30-31 37.70184414794362 40.0 37.0 41.0 32.0 41.0 32-33 37.472749560818 40.0 36.5 41.0 32.0 41.0 34-35 37.44510100103179 40.0 36.0 41.0 32.0 41.0 36-37 37.35948891309077 40.0 36.0 41.0 31.0 41.0 38-39 37.25151044292 40.0 36.0 41.0 31.0 41.0 40-41 37.08581331375269 40.0 36.0 41.0 31.0 41.0 42-43 36.90084215001118 39.5 36.0 41.0 31.0 41.0 44-45 36.65173808794834 39.0 35.0 41.0 30.0 41.0 46-47 36.36836267154584 39.0 35.0 41.0 30.0 41.0 48-49 36.092440272747595 39.0 35.0 41.0 29.0 41.0 50-51 35.846638446190255 39.0 35.0 40.0 28.5 41.0 52-53 35.61699898354313 38.5 35.0 40.0 28.0 41.0 54-55 35.356873810427295 38.0 34.5 40.0 27.5 41.0 56-57 35.0803788842162 38.0 34.0 40.0 27.0 41.0 58-59 34.755694040817524 38.0 34.0 40.0 26.0 41.0 60-61 34.385368251564245 37.0 33.5 40.0 26.0 41.0 62-63 33.99803438362768 37.0 33.0 40.0 25.5 41.0 64-65 33.62452945975867 36.0 33.0 39.0 25.0 41.0 66-67 33.214838537941226 36.0 32.0 39.0 23.5 40.0 68-69 32.750543126456094 35.0 32.0 38.5 22.5 40.0 70-71 32.258807543835744 35.0 31.0 38.0 22.0 40.0 72-73 31.87987918455572 35.0 31.0 37.0 20.5 39.0 74-75 31.442330981145744 35.0 31.0 37.0 19.5 39.0 76-77 29.448479029371974 32.5 28.5 35.0 16.5 37.0 78-79 30.52749814016044 34.0 30.0 36.0 18.0 37.5 80-81 30.586383175922997 34.0 30.0 35.0 18.0 37.0 82-83 30.264646590505137 34.0 30.0 35.0 15.0 37.0 84-85 29.859303012423695 34.0 30.0 35.0 9.0 36.0 86-87 29.297213189085966 34.0 29.0 35.0 4.5 36.0 88-89 28.751245917598027 34.0 29.0 35.0 2.0 35.5 90-91 28.300900885293625 33.5 28.5 35.0 2.0 35.0 92-93 27.977017613588472 33.0 27.0 35.0 2.0 35.0 94-95 27.531254966096448 33.0 27.0 35.0 2.0 35.0 96-97 26.933290145299026 33.0 25.5 35.0 2.0 35.0 98-99 26.09294133834488 33.0 24.5 34.5 2.0 35.0 100-101 22.784276434958254 28.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.007204717063888921 1101 2 0.07218614981877636 1101 3 0.0672564423134503 1101 4 0.0559732181922854 1101 5 0.058893416480991334 1101 6 0.10825225518902215 1101 7 0.08821519466411587 1101 8 0.06393621088223966 1101 9 0.15570814221472773 1101 10-11 0.06283051042954213 1101 12-13 0.1570748331292151 1101 14-15 0.1843699003578152 1101 16-17 0.16729857930891967 1101 18-19 0.23416782231714706 1101 20-21 0.1857808232141238 1101 22-23 0.2218520232192276 1101 24-25 0.05026325781158647 1101 26-27 0.18480675635434807 1101 28-29 0.2233052815579697 1101 30-31 0.056787170692480515 1101 32-33 0.1725669389899096 1101 34-35 0.15386630993438644 1101 36-37 0.050453568151731076 1101 38-39 0.17714374080691897 1101 40-41 0.33835476528457065 1101 42-43 0.222129741008402 1101 44-45 0.2889650642587469 1101 46-47 0.30496355686069165 1101 48-49 0.02751703415787432 1101 50-51 0.12817807743923737 1101 52-53 0.2624470876570655 1101 54-55 0.1739459168752049 1101 56-57 0.0336041069887969 1101 58-59 0.08033281501214873 1101 60-61 0.08648903646791695 1101 62-63 0.0037481553878322416 1101 64-65 0.11763251972283228 1101 66-67 0.10691520538985344 1101 68-69 0.118072834516191 1101 70-71 0.00808900826173442 1101 72-73 -0.04038543389532734 1101 74-75 0.08064936582579563 1101 76-77 -0.04937516529770747 1101 78-79 -0.02091608348308327 1101 80-81 0.19804756842598792 1101 82-83 0.2395571181872107 1101 84-85 0.18875457584784527 1101 86-87 0.27422215413920625 1101 88-89 0.34546349901255624 1101 90-91 0.5309471971677873 1101 92-93 0.5326736541110257 1101 94-95 0.39210873474182506 1101 96-97 0.4462805684590414 1101 98-99 0.5318205348325655 1101 100-101 0.909518742413038 1102 1 0.06887198710666098 1102 2 0.08031220738199352 1102 3 0.062180933292879104 1102 4 0.017967786672443253 1102 5 0.07436972990215907 1102 6 0.048038875673775294 1102 7 0.06402881613507105 1102 8 0.012568741214657564 1102 9 0.10986808946565674 1102 10-11 0.10277635411389952 1102 12-13 0.09586247840761786 1102 14-15 0.09960130738928541 1102 16-17 0.11707994531460031 1102 18-19 0.02419702269438062 1102 20-21 0.2070165223492353 1102 22-23 0.19883303559732468 1102 24-25 0.12459626240897137 1102 26-27 0.17635260594006041 1102 28-29 0.16160453265284502 1102 30-31 0.12077617146760389 1102 32-33 0.1485830224623541 1102 34-35 0.15802205814156167 1102 36-37 0.24926166390808646 1102 38-39 0.18800547537514944 1102 40-41 0.16621967637265556 1102 42-43 0.12030349743889701 1102 44-45 0.10382570707560745 1102 46-47 0.03453657085484707 1102 48-49 0.052228009436852574 1102 50-51 0.009716073678255555 1102 52-53 -0.03385043388572484 1102 54-55 0.08704211015831476 1102 56-57 -0.12599025403591213 1102 58-59 -0.23633466498281308 1102 60-61 -0.18427018468891276 1102 62-63 -0.28190115863595366 1102 64-65 -0.1714352131226491 1102 66-67 -0.156277338924248 1102 68-69 -0.13891011850105173 1102 70-71 -0.03577193302768933 1102 72-73 -0.023090650809983515 1102 74-75 -0.05880575717781511 1102 76-77 -0.13549153782325263 1102 78-79 -0.06438946438288085 1102 80-81 0.04511896238367896 1102 82-83 0.04081552620488438 1102 84-85 0.028684871721551986 1102 86-87 0.02435328914073054 1102 88-89 0.1579828912179977 1102 90-91 0.26300666924584704 1102 92-93 0.37105467307140927 1102 94-95 0.3448605837396741 1102 96-97 0.509446499914759 1102 98-99 0.6899421663766923 1102 100-101 0.9773081615037071 1103 1 -0.010476934334533894 1103 2 -0.028844954044011217 1103 3 -0.08497479687024878 1103 4 0.014538597672725473 1103 5 0.045919729171920665 1103 6 0.08207372254325662 1103 7 0.03301276554431354 1103 8 0.06782132990183953 1103 9 0.05849889666870922 1103 10-11 -0.054791083195325996 1103 12-13 -0.0036007980159808994 1103 14-15 -0.03276568009446379 1103 16-17 -0.05856959974827447 1103 18-19 -0.033085622513084445 1103 20-21 0.030996017317910685 1103 22-23 -0.04345567057024624 1103 24-25 -0.004082302238842317 1103 26-27 -0.03546991746400607 1103 28-29 0.029579295691206653 1103 30-31 0.057410659060892044 1103 32-33 0.031088024580050444 1103 34-35 -0.1283612282132438 1103 36-37 -0.13265111668515317 1103 38-39 0.04028673313391096 1103 40-41 0.13595795973349567 1103 42-43 0.1387711698841656 1103 44-45 0.06251186617946303 1103 46-47 0.001491193979965999 1103 48-49 0.14539811064758368 1103 50-51 0.08095134116376101 1103 52-53 0.10055172177141714 1103 54-55 0.08612712425187397 1103 56-57 0.10712814645480506 1103 58-59 -0.12238861483019292 1103 60-61 -0.050898392251241376 1103 62-63 0.07572834874672907 1103 64-65 -0.03918506342424166 1103 66-67 -0.0976130869051417 1103 68-69 -0.0022595657122792545 1103 70-71 -0.2644760559410386 1103 72-73 -0.009690087067667719 1103 74-75 0.06419668181095162 1103 76-77 0.026035239936678778 1103 78-79 -0.12302888837259829 1103 80-81 -0.008889303889635158 1103 82-83 0.0028030829807477176 1103 84-85 -0.053929665819890005 1103 86-87 0.04490797652663048 1103 88-89 0.3160089168346829 1103 90-91 0.4334613142780519 1103 92-93 0.371716997267054 1103 94-95 0.2954009584968702 1103 96-97 0.2803064260148602 1103 98-99 0.397074382935525 1103 100-101 1.0184211874563012 1104 1 -0.10115281667927434 1104 2 -0.09858084531933997 1104 3 -0.08353436938941883 1104 4 -0.04300475455153219 1104 5 -0.03917139110360068 1104 6 -0.0604812695671626 1104 7 -0.07598430211272245 1104 8 -0.14423864440362166 1104 9 -0.07121141248864404 1104 10-11 -0.008416018498067501 1104 12-13 -0.07428488921225096 1104 14-15 5.354788577704994E-4 1104 16-17 0.010440119567910244 1104 18-19 -0.05991019771457218 1104 20-21 -0.10214386501964157 1104 22-23 -0.07054156425133584 1104 24-25 -0.15055954476621736 1104 26-27 -0.13034061062742808 1104 28-29 -0.05083698369124079 1104 30-31 -0.13301536032287942 1104 32-33 -0.15002333160497017 1104 34-35 -0.0457192168804923 1104 36-37 -0.1524325152612036 1104 38-39 -0.15256237014293106 1104 40-41 -0.01482960436231906 1104 42-43 0.035488764706407494 1104 44-45 -0.0049293801629772815 1104 46-47 -0.10023976973605642 1104 48-49 -0.03703327203238871 1104 50-51 -0.09346241508169584 1104 52-53 -0.08114910459980251 1104 54-55 -0.09321604621298718 1104 56-57 -0.13923587867493126 1104 58-59 -0.1531574746159876 1104 60-61 -0.16523003300077477 1104 62-63 -0.3192473102434974 1104 64-65 -0.07207836544207424 1104 66-67 -0.272208062935114 1104 68-69 -0.19372894788771333 1104 70-71 -0.2804481854174057 1104 72-73 -0.37176002171612765 1104 74-75 -0.38922084002872737 1104 76-77 -0.26782264597939687 1104 78-79 -0.1909248142550588 1104 80-81 -0.09652621363813196 1104 82-83 -0.1199641588676208 1104 84-85 0.009701580697921486 1104 86-87 0.120285289158641 1104 88-89 0.2514693230098253 1104 90-91 0.19779420288094585 1104 92-93 0.2616798269757652 1104 94-95 0.3017655727033315 1104 96-97 0.2696205293658984 1104 98-99 0.201675386884137 1104 100-101 0.6458229116973477 1105 1 -0.02963561896389777 1105 2 -0.019532521093914568 1105 3 -0.03942406041806379 1105 4 0.0023939410994699983 1105 5 -0.03567644941299619 1105 6 -0.03796099474710957 1105 7 -0.006205520989567503 1105 8 -0.01045031808565966 1105 9 -0.02268665212454124 1105 10-11 0.026619246140981545 1105 12-13 0.014967578239392765 1105 14-15 0.1264942236566 1105 16-17 0.02047994486777327 1105 18-19 -0.09917480457219341 1105 20-21 -0.011907166723446494 1105 22-23 -0.02268245916030054 1105 24-25 -0.004050663549968192 1105 26-27 0.004608672141586112 1105 28-29 0.08413309032560079 1105 30-31 0.06120976999130079 1105 32-33 -0.007572097141398615 1105 34-35 0.20329856433119886 1105 36-37 0.11249627136157159 1105 38-39 0.04412887043729086 1105 40-41 0.10541490071208415 1105 42-43 0.17152355181744383 1105 44-45 4.2570752242454546E-4 1105 46-47 0.09964125067156715 1105 48-49 0.06740015975756108 1105 50-51 0.17624735251239798 1105 52-53 0.12101157552886832 1105 54-55 0.15441777564760173 1105 56-57 0.10251871484945951 1105 58-59 0.2604699810986517 1105 60-61 0.16950939230230944 1105 62-63 0.10192669774570362 1105 64-65 -0.03341133928802975 1105 66-67 0.09749754640940722 1105 68-69 0.1134603973355297 1105 70-71 0.17103677915699222 1105 72-73 -0.03172519972945054 1105 74-75 -0.02137738873524242 1105 76-77 0.06568104144900744 1105 78-79 -0.12712318153848656 1105 80-81 0.011542333093881751 1105 82-83 0.1262595469107346 1105 84-85 0.20618792688427234 1105 86-87 0.2465344407415948 1105 88-89 0.35364925180985907 1105 90-91 0.4848535960984357 1105 92-93 0.36875810669450715 1105 94-95 0.25970219561448005 1105 96-97 0.22310715455737906 1105 98-99 0.37694359398106414 1105 100-101 0.9362112519655668 1106 1 -0.08369310384555817 1106 2 -0.13841185442322512 1106 3 -0.07890259370857677 1106 4 -0.06403053269477965 1106 5 -0.10834369759285778 1106 6 -0.11560418119807991 1106 7 -0.08323769395599356 1106 8 -0.14443033343027878 1106 9 -0.09204986835292317 1106 10-11 -0.045307086729948765 1106 12-13 0.009311736613014432 1106 14-15 0.10478553361006249 1106 16-17 -0.0878614731081413 1106 18-19 -0.206032548162419 1106 20-21 -0.20227943574012386 1106 22-23 -0.10325651012830406 1106 24-25 -0.14123939363643956 1106 26-27 -0.1176252012034098 1106 28-29 -0.02755158157199844 1106 30-31 -0.04287874606166753 1106 32-33 -0.10145641981819864 1106 34-35 -0.08064955941713237 1106 36-37 -0.01767748084899523 1106 38-39 -0.13804282211698649 1106 40-41 -0.15671027525599612 1106 42-43 -0.08711142752006396 1106 44-45 0.039513194417708064 1106 46-47 0.033330196534741674 1106 48-49 0.12939215981656815 1106 50-51 0.06670882636900188 1106 52-53 -0.062092057855892335 1106 54-55 0.09187460075091991 1106 56-57 -0.15276809385213852 1106 58-59 -0.10267232894801026 1106 60-61 0.02422629795481157 1106 62-63 0.04586894825542487 1106 64-65 0.06424198006396153 1106 66-67 -0.15943909926959776 1106 68-69 -0.16363388267090073 1106 70-71 -0.06731971525259439 1106 72-73 0.03657410658922089 1106 74-75 0.26115405085856125 1106 76-77 0.0989672525463412 1106 78-79 -0.056709241133980015 1106 80-81 -0.03329117243618995 1106 82-83 0.07754859763401711 1106 84-85 -0.06369085152679332 1106 86-87 0.01977731323800569 1106 88-89 0.32021732718219553 1106 90-91 0.3434926428995233 1106 92-93 0.49763345525043334 1106 94-95 0.40821490073923883 1106 96-97 0.360885108144938 1106 98-99 0.3922772053322383 1106 100-101 1.006000378562689 1107 1 -0.05118144835874716 1107 2 -0.06127798542743079 1107 3 -0.09500667497111692 1107 4 -0.0442684976454899 1107 5 -0.06204094508217395 1107 6 -0.07562980650180862 1107 7 -0.026905542106781866 1107 8 0.021321125124821094 1107 9 -0.1025789207382033 1107 10-11 -0.01031443796652809 1107 12-13 -0.022609251585194556 1107 14-15 -0.08998314946040153 1107 16-17 -0.05623192114830999 1107 18-19 -0.01656506615469766 1107 20-21 -0.011957440474660075 1107 22-23 -0.062185926929451796 1107 24-25 -0.10523257816700493 1107 26-27 0.044472733709795875 1107 28-29 0.02039991383740869 1107 30-31 -0.012194609445863591 1107 32-33 0.07430368929571074 1107 34-35 -0.04771922720225774 1107 36-37 0.06264827947881457 1107 38-39 0.07271599216090863 1107 40-41 -0.027432143247331453 1107 42-43 -0.05393132485959029 1107 44-45 -0.10053590953221203 1107 46-47 -0.07365684848797827 1107 48-49 -0.20455860860047181 1107 50-51 -0.09187314370407762 1107 52-53 -0.14327031264126333 1107 54-55 -0.22887803711996213 1107 56-57 -0.10948765345760592 1107 58-59 -0.0730369590303539 1107 60-61 -0.17023334045340732 1107 62-63 -0.11045162913937645 1107 64-65 -0.10201720673865111 1107 66-67 -0.06816252555035618 1107 68-69 0.053079382764202876 1107 70-71 -0.028924547004102408 1107 72-73 0.07274096805141284 1107 74-75 0.10160961779541822 1107 76-77 0.09400268641510579 1107 78-79 0.12843430733578742 1107 80-81 0.14432269995118574 1107 82-83 0.17627179623898215 1107 84-85 0.1367045641368776 1107 86-87 0.1984253160221563 1107 88-89 0.3017747462897127 1107 90-91 0.43666004730680896 1107 92-93 0.5634475190425015 1107 94-95 0.5339528983832977 1107 96-97 0.5049711395392968 1107 98-99 0.7309934637507745 1107 100-101 1.0642432126351444 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sequence quality scores pass #Quality Count 2 4.0 3 16.0 4 41.0 5 101.0 6 222.0 7 552.0 8 1247.0 9 2319.0 10 3715.0 11 5335.0 12 7372.0 13 10125.0 14 12456.0 15 16055.0 16 19440.0 17 24709.0 18 24221.0 19 18946.0 20 17942.0 21 18978.0 22 20386.0 23 22864.0 24 26624.0 25 31605.0 26 38709.0 27 47272.0 28 57066.0 29 69349.0 30 84282.0 31 100921.0 32 125176.0 33 155780.0 34 204561.0 35 284999.0 36 421808.0 37 616685.0 38 468921.0 39 40799.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.10569965374966 19.754427784031282 14.984272596730385 23.155599965488666 2 16.450643206313426 20.83296825063141 38.31912481430756 24.397263728747607 3 19.879111261549244 23.930213289365714 29.855180715104563 26.335494733980475 4 13.80045928792049 17.12858096157287 35.86393670315495 33.2070230473517 5 13.92875740062893 37.13665664646524 34.15581607561027 14.778769877295565 6 32.60088026297948 35.94466023654694 16.810284371384224 14.644175129089357 7 28.62377203114469 32.30313935587085 20.409061424845323 18.664027188139137 8 26.505637154547085 34.96948130715488 19.270969545272976 19.25391199302506 9 25.9323434844648 16.740121861551977 20.081902903215383 37.245631750767835 10-11 25.31109210645112 26.352085868784116 26.921864750268437 21.414957274496327 12-13 26.06370662609279 23.571288408227204 27.48259846488693 22.882406500793078 14-15 22.740196142122915 25.32203668777875 25.75962012247472 26.178147047623618 16-17 22.371878931320495 28.361595529755434 26.574044089426213 22.692481449497855 18-19 22.23018278742198 26.943316759403274 28.777587564505147 22.048912888669598 20-21 22.878362474711427 26.031961260693247 28.96099666761338 22.128679596981947 22-23 22.53983954573606 25.961377970371164 28.933773053931517 22.565009429961258 24-25 22.60923579833842 26.138733203558235 28.9571772149748 22.29485378312855 26-27 22.389707082693953 26.271755911351217 28.95246132226901 22.386075683685824 28-29 22.192491811875186 26.669049837703383 28.880718069644786 22.257740280776638 30-31 22.433529683972196 26.237213915364556 28.78811754918955 22.541138851473697 32-33 22.25968923938309 26.333212620056685 28.831461055975755 22.57563708458447 34-35 22.800078630496635 26.39555804330008 28.556997114660586 22.24736621154269 36-37 22.78144088884979 26.19139966385569 28.608853044158593 22.418306403135926 38-39 22.429714877847033 26.46680599715286 28.67981171225728 22.423667412742827 40-41 22.66499454667429 26.349500288117518 28.650742100424154 22.334763064784045 42-43 22.488197528560818 26.473001742467904 28.307324029065562 22.731476699905713 44-45 22.644981856028956 26.455782149744067 28.276176159768223 22.623059834458754 46-47 22.846060996968575 26.70090776159989 27.89195548046865 22.561075760962886 48-49 22.78866798274188 26.770125881133787 27.74142403646853 22.6997820996558 50-51 22.62753380587233 26.94098604188152 27.791902715447314 22.639577436798838 52-53 22.93917616686817 26.92526293450533 27.54188345360796 22.593677445018546 54-55 22.673151645970503 26.81400571627893 27.625455464963224 22.887387172787342 56-57 22.719576839442123 27.044749089070073 27.465307703916874 22.770366367570926 58-59 22.851568193596027 26.747169542281 27.556940228855336 22.844322035267638 60-61 22.611613244551247 26.81757933276175 27.91075304801819 22.66005437466882 62-63 22.516402069161046 26.85634975711312 27.899042611564557 22.728205562161286 64-65 22.436961183740824 26.916484291893365 27.7527207961879 22.89383372817791 66-67 22.407086556854154 27.00714509287721 27.477698758033505 23.108069592235132 68-69 22.513653628768555 26.745937060544385 27.678361717800243 23.06204759288682 70-71 22.520374527492866 26.715035138605426 27.5470741372268 23.217516196674907 72-73 22.515485892038352 26.743993126339493 27.57453267470748 23.165988306914674 74-75 22.37252894536686 27.118509676329616 27.29616474930229 23.212796629001236 76-77 22.480930362224743 26.82824754080539 27.424913613890606 23.26590848307926 78-79 22.45007734372826 26.93541369604706 27.422705012536795 23.191803947687877 80-81 22.548666528296067 26.925697319530478 27.341825245927094 23.183810906246364 82-83 22.468011259317105 26.887899565665414 27.469155647832178 23.174933527185306 84-85 22.364799742004525 26.925046383549056 27.388082301356974 23.322071573089445 86-87 22.417401850185207 26.828536325169512 27.386227622326004 23.36783420231928 88-89 22.349448295557373 26.893575877972143 27.153640885033152 23.603334941437332 90-91 22.28560433261043 26.88200438018042 27.240867163479077 23.591524123730075 92-93 22.40634421007708 26.7340151245851 27.30760863445299 23.55203203088483 94-95 22.193361712618508 26.596726248728803 27.413606565159775 23.79630547349291 96-97 22.160549599847016 26.498816298763128 27.49908215679494 23.84155194459492 98-99 22.060097361536997 26.58249144788306 27.21593778776358 24.14147340281637 100-101 22.195366009551964 26.05606411153587 27.688536859652153 24.060033019260015 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1263.0 1 863.5 2 599.0 3 774.0 4 684.5 5 635.5 6 1108.0 7 2364.0 8 3655.0 9 4079.0 10 3412.0 11 2525.0 12 2400.5 13 2540.0 14 2782.0 15 3081.5 16 3241.5 17 3523.5 18 3947.0 19 4413.5 20 4972.5 21 5766.5 22 6901.5 23 8462.5 24 10270.0 25 12764.5 26 16283.5 27 20476.0 28 24574.0 29 28811.5 30 33455.5 31 39035.0 32 45547.5 33 49938.5 34 53371.5 35 57894.5 36 63574.5 37 69757.5 38 74413.5 39 77550.5 40 80877.5 41 85670.5 42 89612.0 43 93794.0 44 99019.0 45 102948.0 46 105292.5 47 110284.0 48 119917.0 49 121093.0 50 115016.0 51 111091.5 52 108539.0 53 108291.0 54 107207.5 55 101100.0 56 92503.5 57 83743.5 58 76863.5 59 68982.0 60 58010.0 61 48020.5 62 39555.0 63 30738.0 64 22685.5 65 17404.5 66 13226.0 67 10453.5 68 7839.0 69 5384.0 70 3955.5 71 2864.0 72 2184.0 73 1780.0 74 1384.0 75 1114.5 76 788.0 77 462.0 78 306.0 79 208.5 80 118.0 81 66.0 82 48.0 83 34.5 84 18.0 85 8.5 86 8.0 87 6.5 88 2.0 89 1.5 90 1.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3758325134936233 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.332621269368401E-4 16-17 0.0022321406261920714 18-19 4.9973297601315035E-5 20-21 1.6657765867105012E-5 22-23 0.0 24-25 0.0 26-27 1.665776586710501E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007429363576728834 36-37 0.01702423671618132 38-39 0.011510516214169562 40-41 0.007446021342595939 42-43 0.0037646550859657324 44-45 0.0018323542453815512 46-47 0.0014658833963052409 48-49 4.9973297601315035E-5 50-51 5.497062736144654E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.164441466776253E-4 60-61 7.162839322855155E-4 62-63 0.0 64-65 0.0 66-67 0.0010827547813618255 68-69 6.663106346842004E-4 70-71 0.002032247435786811 72-73 0.0 74-75 0.0 76-77 0.09518247416463803 78-79 0.03453154864250869 80-81 6.663106346842005E-5 82-83 0.0 84-85 0.0 86-87 0.14443948783366753 88-89 0.3153814811618992 90-91 0.2704888021500511 92-93 0.0 94-95 1.6657765867105012E-5 96-97 3.3315531734210023E-5 98-99 2.3320872213947015E-4 100-101 7.162839322855155E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3001603.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.57846122929915 #Duplication Level Percentage of deduplicated Percentage of total 1 76.52059666749085 43.29417611794469 2 13.705931748122103 15.509210560450933 3 4.18284822517421 7.099773484081854 4 1.8007266697332867 4.075293762722789 5 0.983257942842331 2.7815610698752633 6 0.5919236641135666 2.0094078048452473 7 0.40267370816192816 1.5947861149708438 8 0.287448693147691 1.3010723792535477 9 0.2198316837932827 1.1193964558622815 >10 1.189966096199354 12.276879214584698 >50 0.07775572355442385 3.0373203609310124 >100 0.03374296283303568 3.435728510564807 >500 0.002354439167323061 0.945728265208704 >1k 7.651927292228553E-4 0.7178050223671313 >5k 1.7658293751296662E-4 0.8018608763364474 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9615 0.3203288376244293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8841 0.2945426160621508 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5622 0.18729991940972873 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4152 0.13832608776044 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.3315531734210023E-5 2 0.0 0.0 0.0 0.0 3.3315531734210023E-5 3 0.0 0.0 0.0 0.0 3.3315531734210023E-5 4 0.0 0.0 0.0 0.0 3.3315531734210023E-5 5 0.0 0.0 0.0 3.3315531734210023E-5 3.3315531734210023E-5 6 0.0 0.0 0.0 3.3315531734210023E-5 3.3315531734210023E-5 7 0.0 0.0 0.0 3.3315531734210023E-5 3.3315531734210023E-5 8 0.0 0.0 0.0 3.3315531734210023E-5 3.3315531734210023E-5 9 0.0 0.0 0.0 9.994659520263006E-5 6.663106346842005E-5 10-11 0.0 0.0 0.0 9.994659520263006E-5 6.663106346842005E-5 12-13 0.0 0.0 0.0 1.499198928039451E-4 1.332621269368401E-4 14-15 0.0 0.0 0.0 2.1655095627236515E-4 1.832354245381551E-4 16-17 0.0 0.0 0.0 3.1649755147499523E-4 1.998931904052601E-4 18-19 0.0 0.0 0.0 4.1644414667762526E-4 1.998931904052601E-4 20-21 0.0 0.0 0.0 4.4975967841183527E-4 2.1655095627236515E-4 22-23 0.0 0.0 0.0 7.162839322855153E-4 2.665242538736802E-4 24-25 0.0 0.0 0.0 9.828081861591956E-4 2.998397856078902E-4 26-27 0.0 0.0 0.0 0.0013659368011026108 2.998397856078902E-4 28-29 0.0 3.3315531734210023E-5 0.0 0.0027485313680723268 2.998397856078902E-4 30-31 0.0 3.3315531734210023E-5 0.0 0.00771254559646962 3.1649755147499523E-4 32-33 0.0 3.3315531734210023E-5 0.0 0.017773836180201046 4.1644414667762526E-4 34-35 0.0 3.3315531734210023E-5 0.0 0.034914677257452106 4.664174442789403E-4 36-37 0.0 3.3315531734210023E-5 0.0 0.06296635497765694 4.664174442789403E-4 38-39 0.0 3.3315531734210023E-5 0.0 0.10677627920814312 4.664174442789403E-4 40-41 0.0 3.3315531734210023E-5 0.0 0.15345133916777134 4.664174442789403E-4 42-43 0.0 9.994659520263006E-5 0.0 0.20165891358717325 4.664174442789403E-4 44-45 0.0 9.994659520263006E-5 0.0 0.2652082903701789 4.997329760131503E-4 46-47 0.0 9.994659520263006E-5 0.0 0.342966741437825 5.330485077473604E-4 48-49 0.0 9.994659520263006E-5 0.0 0.4309030874502724 5.996795712157804E-4 50-51 0.0 9.994659520263006E-5 0.0 0.5243864694964657 5.996795712157804E-4 52-53 0.0 9.994659520263006E-5 0.0 0.6145549561351051 6.663106346842004E-4 54-55 0.0 9.994659520263006E-5 0.0 0.7204816892840259 7.662572298868305E-4 56-57 0.0 9.994659520263006E-5 0.0 0.8539270516454042 7.995727616210405E-4 58-59 0.0 9.994659520263006E-5 0.0 1.0032972381757346 7.995727616210405E-4 60-61 0.0 9.994659520263006E-5 0.0 1.1622789556113848 7.995727616210405E-4 62-63 0.0 9.994659520263006E-5 0.0 1.327224153227459 7.995727616210405E-4 64-65 0.0 9.994659520263006E-5 0.0 1.4936852075374392 7.995727616210405E-4 66-67 0.0 9.994659520263006E-5 0.0 1.6972597641993294 7.995727616210405E-4 68-69 0.0 1.1660436106973508E-4 0.0 1.9272202219947143 7.995727616210405E-4 70-71 0.0 1.332621269368401E-4 0.0 2.18013508115497 8.495460592223556E-4 72-73 0.0 1.332621269368401E-4 0.0 2.4280526105550932 9.661504202920907E-4 74-75 0.0 1.332621269368401E-4 0.0 2.6895129036051735 9.994659520263006E-4 76-77 0.0 1.332621269368401E-4 0.0 2.97654286726126 9.994659520263006E-4 78-79 0.0 1.665776586710501E-4 0.0 3.3094483181153533 0.0010161237178934056 80-81 0.0 1.665776586710501E-4 0.0 3.667840150746118 0.0010660970154947207 82-83 0.0 1.665776586710501E-4 0.0 4.027947733261194 0.0012326746741657707 84-85 0.0 1.665776586710501E-4 0.0 4.399649120819776 0.0012493324400328757 86-87 0.0 1.665776586710501E-4 0.0 4.786625679678492 0.0013159635035012956 88-89 0.0 1.665776586710501E-4 0.0 5.2086002046239965 0.001382594566969716 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11120 0.0 29.595749 1 GTATCAA 15330 0.0 25.879683 1 TCAACGC 18400 0.0 21.457266 4 ATCAACG 18445 0.0 21.430643 3 GTACATG 10620 0.0 21.39193 1 TATCAAC 18590 0.0 21.391119 2 CAACGCA 18760 0.0 21.0708 5 TACATGG 10650 0.0 20.986506 2 AACGCAG 19155 0.0 20.735388 6 GAGTACT 10800 0.0 20.4767 12-13 ACATGGG 10370 0.0 20.409151 3 GTACTTT 11390 0.0 19.353514 14-15 AGAGTAC 16505 0.0 19.019753 10-11 AGTACTT 11370 0.0 18.803215 12-13 ACGCAGA 21195 0.0 18.62768 7 CGCAGAG 21245 0.0 18.449821 8 CATGGGG 6640 0.0 17.938019 4 GTGTAGC 1915 0.0 17.409462 1 GCAGAGT 22835 0.0 16.895008 9 TACCTGG 2365 0.0 16.653894 2 >>END_MODULE