FastQCFastQC Report
Thu 26 May 2016
SRR938678_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938678_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003594
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99010.32963842649838826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83520.2780668758826925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51420.17119490849961747No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36580.12178743198980956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97800.029.0494271
GTATCAA137400.028.214911
GTACATG101200.023.9891911
TATCAAC165950.023.4122642
TCAACGC165850.023.3691734
ATCAACG165700.023.3616983
TACATGG102800.023.3503932
CAACGCA167700.023.0830865
ACATGGG102400.022.6540413
AACGCAG171950.022.5669786
GTGTACG3450.022.0331271
GAGTACT95450.021.78118512-13
CATGGGG65950.020.5725444
TACCTGG22850.020.3458882
ACGCAGA189400.020.287787
AGAGTAC149200.019.92946810-11
GTACTTT104900.019.86459214-15
CGCAGAG194250.019.7079778
AGTACTT103750.019.60406312-13
GTACCTG24750.019.5794371
Produced by FastQC (version 0.11.5)