##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938678_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3003594 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59544499023503 33.0 31.0 34.0 30.0 34.0 2 31.687380851073748 33.0 31.0 34.0 30.0 34.0 3 31.706925769594694 33.0 31.0 34.0 30.0 34.0 4 35.25077890021088 37.0 35.0 37.0 33.0 37.0 5 35.27367913239939 37.0 35.0 37.0 33.0 37.0 6 35.320584939242785 37.0 35.0 37.0 33.0 37.0 7 35.22880655641209 37.0 35.0 37.0 33.0 37.0 8 35.19897895654339 37.0 35.0 37.0 32.0 37.0 9 36.78919154852487 39.0 37.0 39.0 33.0 39.0 10-11 36.672220013756856 39.0 37.0 39.0 32.0 39.0 12-13 36.561022062236106 39.0 37.0 39.0 32.0 39.0 14-15 37.74440104088635 40.0 38.0 41.0 32.0 41.0 16-17 37.62009745658035 40.0 38.0 41.0 32.0 41.0 18-19 37.54223640079185 40.0 37.0 41.0 32.0 41.0 20-21 37.42075560145612 40.0 37.0 41.0 32.0 41.0 22-23 37.44995628570306 40.0 37.0 41.0 32.0 41.0 24-25 37.38523615375447 40.0 37.0 41.0 32.0 41.0 26-27 37.25051022208727 40.0 37.0 41.0 31.0 41.0 28-29 37.07418712382566 40.0 37.0 41.0 31.0 41.0 30-31 36.847963806027046 40.0 36.5 41.0 30.0 41.0 32-33 36.66030811754185 39.0 36.0 41.0 30.0 41.0 34-35 36.373998616324315 39.0 35.5 41.0 30.0 41.0 36-37 36.16901651821118 39.0 35.0 41.0 29.5 41.0 38-39 35.99253078145715 39.0 35.0 41.0 29.0 41.0 40-41 35.88521950703058 39.0 35.0 41.0 28.0 41.0 42-43 35.88051963747431 39.0 35.0 41.0 27.5 41.0 44-45 35.7150060893716 39.0 35.0 41.0 27.0 41.0 46-47 35.43156615041846 39.0 35.0 41.0 26.0 41.0 48-49 35.163373778213696 39.0 35.0 41.0 25.0 41.0 50-51 34.03420419004699 38.0 33.5 40.0 23.5 40.5 52-53 34.11247242470188 38.0 33.5 39.5 23.5 40.5 54-55 34.595307821230165 38.0 34.0 40.0 24.0 41.0 56-57 34.47162282918397 38.0 34.0 40.0 23.0 41.0 58-59 34.25058363413963 38.0 34.0 40.0 23.0 41.0 60-61 33.93316223830518 38.0 34.0 40.0 22.0 41.0 62-63 33.535141733536555 37.0 33.0 40.0 21.5 41.0 64-65 33.141921145134795 36.5 33.0 39.5 19.5 41.0 66-67 32.7481438902861 36.0 32.0 39.0 18.5 41.0 68-69 32.32100876483306 35.5 32.0 39.0 16.5 40.5 70-71 31.894036111405203 35.0 31.0 38.5 13.0 40.0 72-73 31.47290362812018 35.0 31.0 37.5 9.5 40.0 74-75 31.00684380112625 35.0 31.0 37.0 7.0 39.0 76-77 30.551151720239154 35.0 30.0 36.5 4.5 39.0 78-79 30.12029338852055 34.0 30.0 36.0 2.0 38.5 80-81 29.66279097641026 34.0 29.5 35.5 2.0 37.0 82-83 29.25222633285324 34.0 29.0 35.0 2.0 37.0 84-85 28.905148465471697 34.0 29.0 35.0 2.0 36.5 86-87 28.49285539257303 34.0 29.0 35.0 2.0 36.0 88-89 28.04676780550234 34.0 27.5 35.0 2.0 36.0 90-91 27.601608273288598 34.0 27.0 35.0 2.0 35.0 92-93 27.137045153239754 33.5 26.0 35.0 2.0 35.0 94-95 26.61921135146761 33.0 25.0 35.0 2.0 35.0 96-97 26.011082223496253 33.0 24.0 35.0 2.0 35.0 98-99 25.237729200417903 32.5 19.0 35.0 2.0 35.0 100-101 22.746125641481505 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05806650527599899 1101 2 0.14891035917660744 1101 3 0.18224058731262716 1101 4 0.08432444504715875 1101 5 -0.002582886326706557 1101 6 0.1067189335860732 1101 7 0.17951893858430878 1101 8 0.19062356323097163 1101 9 0.20886288651696105 1101 10-11 0.12749781237889835 1101 12-13 0.20947045280887266 1101 14-15 0.17831982108896227 1101 16-17 0.30329316532124295 1101 18-19 0.3219772796026419 1101 20-21 0.4085353291600242 1101 22-23 0.40101385935756895 1101 24-25 0.2879333426444788 1101 26-27 0.35925115462853796 1101 28-29 0.3145891223935422 1101 30-31 0.33173180390730295 1101 32-33 0.3209783184235704 1101 34-35 0.3173443242183396 1101 36-37 0.3691691475412924 1101 38-39 0.37765778927628446 1101 40-41 0.6303839659986821 1101 42-43 0.7140816054782775 1101 44-45 0.7072163967393408 1101 46-47 0.5202142518284134 1101 48-49 0.534753999537692 1101 50-51 0.5980710118059065 1101 52-53 0.49595868981175784 1101 54-55 0.40907899837768724 1101 56-57 0.3884250084039138 1101 58-59 0.4596460860444651 1101 60-61 0.2836476807467889 1101 62-63 0.2673587919866236 1101 64-65 0.3934212579070575 1101 66-67 0.49690345515836754 1101 68-69 0.6041195456088957 1101 70-71 0.3903242393693773 1101 72-73 0.3682277956043123 1101 74-75 0.3065160502966471 1101 76-77 0.19641632321421554 1101 78-79 0.13237865503302615 1101 80-81 0.10567422802375859 1101 82-83 0.26007074186136236 1101 84-85 0.22088423342304608 1101 86-87 -0.036781759433235806 1101 88-89 0.07335850999866267 1101 90-91 0.07081551414578158 1101 92-93 -0.053998348521847817 1101 94-95 -0.18640341345565759 1101 96-97 -0.3249884520241615 1101 98-99 -0.27519083167778646 1101 100-101 0.03959911924991033 1102 1 0.21687620440909328 1102 2 0.24865981228068534 1102 3 0.12162240255573309 1102 4 0.24738561786540458 1102 5 0.2746707783810649 1102 6 0.28476971871325674 1102 7 0.09543581298330395 1102 8 0.21033019316996615 1102 9 0.3623178630871635 1102 10-11 0.2686436007107247 1102 12-13 0.3025199203994049 1102 14-15 0.4386548536791963 1102 16-17 0.5933637964621283 1102 18-19 0.5755747134797176 1102 20-21 0.5034494979878374 1102 22-23 0.5350890646934019 1102 24-25 0.40083613165248266 1102 26-27 0.4729118496087068 1102 28-29 0.49055516906572194 1102 30-31 0.5366144485751931 1102 32-33 0.5488209198642267 1102 34-35 0.47114695421922903 1102 36-37 0.548881468894713 1102 38-39 0.5412962172422766 1102 40-41 0.5248842069380899 1102 42-43 0.6712622554429686 1102 44-45 0.6482930627569132 1102 46-47 0.715817129087732 1102 48-49 0.6586674079340753 1102 50-51 0.6468929986339802 1102 52-53 0.49193370132139336 1102 54-55 0.41599649565126384 1102 56-57 0.6749779993840548 1102 58-59 0.4697218562230887 1102 60-61 0.3954465760793582 1102 62-63 0.26081312805776946 1102 64-65 0.31788084220168145 1102 66-67 0.22300504905531682 1102 68-69 0.2952883639655184 1102 70-71 0.32288452473105167 1102 72-73 0.144104870939465 1102 74-75 0.011921303506557734 1102 76-77 0.09074327476970012 1102 78-79 0.15748422991102018 1102 80-81 0.06399235487782207 1102 82-83 0.021437397050629414 1102 84-85 0.029164919023553892 1102 86-87 -0.10610277884981656 1102 88-89 0.004944291091561581 1102 90-91 -0.18563971690458558 1102 92-93 -0.1150843877487624 1102 94-95 -0.15815144069707188 1102 96-97 -0.18032357273674293 1102 98-99 -0.35530565725355956 1102 100-101 0.03738354059340665 1103 1 -0.019373484056771417 1103 2 0.020555787853016483 1103 3 0.09632201582954636 1103 4 -0.0433210856710744 1103 5 0.04691007728938246 1103 6 -0.03870952845072395 1103 7 0.0665839370815462 1103 8 0.02616858875643402 1103 9 0.0533120257427413 1103 10-11 0.025064382877580726 1103 12-13 0.0318274838548831 1103 14-15 0.15478219188065623 1103 16-17 0.1894326594939173 1103 18-19 0.1774211572433515 1103 20-21 0.24119794116485593 1103 22-23 0.12410263317072179 1103 24-25 0.08548199151213964 1103 26-27 0.10928899156485272 1103 28-29 0.17045151084960963 1103 30-31 0.24781891160901637 1103 32-33 0.059408630075594715 1103 34-35 0.25707828446575576 1103 36-37 0.3390174503671304 1103 38-39 0.22497653320147037 1103 40-41 0.3719133156707102 1103 42-43 0.4153225498024611 1103 44-45 0.4013005327945294 1103 46-47 0.3494192860059897 1103 48-49 0.24416466728137465 1103 50-51 0.28252012745058863 1103 52-53 0.0920018573614314 1103 54-55 0.15869430499406434 1103 56-57 0.18503207492874907 1103 58-59 0.3420697894166054 1103 60-61 0.3533621287112467 1103 62-63 0.2644837207609996 1103 64-65 0.031136344276028183 1103 66-67 -0.012057842693565135 1103 68-69 -0.017678195493843418 1103 70-71 -0.0030104895359421846 1103 72-73 0.02440285864086178 1103 74-75 -0.15317759056844338 1103 76-77 -0.10378627053456668 1103 78-79 -0.08486983223982847 1103 80-81 0.02626832580468985 1103 82-83 0.10906326108102604 1103 84-85 0.08548223794804599 1103 86-87 -0.04136662071423913 1103 88-89 -0.0381915310110017 1103 90-91 -0.057752198106573616 1103 92-93 -0.23745287795684433 1103 94-95 -0.18365760949686205 1103 96-97 -0.1325049898737447 1103 98-99 -0.0727346571399643 1103 100-101 0.17682939977488843 1104 1 0.14248966632803572 1104 2 0.30044452100992913 1104 3 0.19858479583086108 1104 4 0.30658547514282475 1104 5 0.2619652553213925 1104 6 0.23644146691273704 1104 7 0.30863401643738797 1104 8 0.2930272780104204 1104 9 0.3671804080473109 1104 10-11 0.2540523724002455 1104 12-13 0.36581438550254575 1104 14-15 0.4602551729448834 1104 16-17 0.5319226957395529 1104 18-19 0.3366185922196294 1104 20-21 0.3561175986444667 1104 22-23 0.3569475190619116 1104 24-25 0.32494162122984704 1104 26-27 0.33234511073523265 1104 28-29 0.3518464448940293 1104 30-31 0.42971751491245413 1104 32-33 0.3257779864077861 1104 34-35 0.43063124205212944 1104 36-37 0.5289127782391532 1104 38-39 0.40381285270951395 1104 40-41 0.5141588752010833 1104 42-43 0.4360025054104142 1104 44-45 0.6380796891095173 1104 46-47 0.698119486208725 1104 48-49 0.7421561042714799 1104 50-51 0.8153035357545306 1104 52-53 0.7985547441094241 1104 54-55 0.7746466004103141 1104 56-57 0.8223037687585588 1104 58-59 0.8501318708166465 1104 60-61 0.7933377822373942 1104 62-63 0.7365204841895121 1104 64-65 0.8836875376496067 1104 66-67 0.9160637682384802 1104 68-69 0.796627445095865 1104 70-71 0.9401328620298415 1104 72-73 0.8023711550960755 1104 74-75 0.6968472450274987 1104 76-77 0.6569735722042473 1104 78-79 0.5188778128059575 1104 80-81 0.5655882605659244 1104 82-83 0.5430243994873969 1104 84-85 0.5833037471764264 1104 86-87 0.6979688310237471 1104 88-89 0.7223983839660839 1104 90-91 0.5863330508144067 1104 92-93 0.6393543438465308 1104 94-95 0.6252746454065843 1104 96-97 0.3991770504063048 1104 98-99 0.386872291092363 1104 100-101 0.7284895476246902 1105 1 -0.13715465174040986 1105 2 -0.0387478365676408 1105 3 0.02567498838797988 1105 4 0.036042723471666704 1105 5 -0.06213930419172442 1105 6 0.056999184163061045 1105 7 -0.0163797020101768 1105 8 0.04057791155327806 1105 9 0.011111865694722667 1105 10-11 0.14144075412229284 1105 12-13 0.09996396646633343 1105 14-15 0.1493920331663645 1105 16-17 0.0680595809036788 1105 18-19 0.03409003290605739 1105 20-21 0.15634769442416996 1105 22-23 0.11801768268558277 1105 24-25 0.20502181179155343 1105 26-27 0.22033959841357387 1105 28-29 0.2703139695872281 1105 30-31 0.27453817738875586 1105 32-33 0.21659036793028008 1105 34-35 0.1905895878567776 1105 36-37 0.3068073706765517 1105 38-39 0.2309610368859083 1105 40-41 0.1779290787329586 1105 42-43 0.20443261615569952 1105 44-45 0.03735759991466381 1105 46-47 0.12158364364661622 1105 48-49 0.10159439620004918 1105 50-51 0.11763941323632565 1105 52-53 0.21685764743178737 1105 54-55 0.12529136744613112 1105 56-57 0.038045687345807266 1105 58-59 0.03894447682282731 1105 60-61 0.06942528099026646 1105 62-63 -0.03489525959977158 1105 64-65 0.05276241543071336 1105 66-67 0.036194966482518964 1105 68-69 -0.038911440467337854 1105 70-71 -0.04993435836325588 1105 72-73 0.1279760139207795 1105 74-75 -0.07982141704972534 1105 76-77 -0.10939253574746388 1105 78-79 0.020424255986640105 1105 80-81 0.1164749711316233 1105 82-83 0.07704365519925815 1105 84-85 0.09519327625953977 1105 86-87 0.19520643453566677 1105 88-89 0.2403745388099452 1105 90-91 0.1807010045208841 1105 92-93 0.27141061442420167 1105 94-95 0.2911369121929752 1105 96-97 0.2456651278282287 1105 98-99 0.12593619411540757 1105 100-101 0.5733869086942285 1106 1 0.08147086246215096 1106 2 0.18359456949384878 1106 3 0.20077335757322246 1106 4 0.14678366293803435 1106 5 0.2301675751474903 1106 6 0.2017043204978748 1106 7 0.20559910390172575 1106 8 0.06022817251615464 1106 9 0.07977315051009981 1106 10-11 0.10969972116454585 1106 12-13 0.10281738418579067 1106 14-15 0.35504917590737506 1106 16-17 0.3841323741007585 1106 18-19 0.35803551117540877 1106 20-21 0.3854652781192769 1106 22-23 0.4068112171473146 1106 24-25 0.28267178415062943 1106 26-27 0.21735000847486674 1106 28-29 0.23045127996233106 1106 30-31 0.1252789949098485 1106 32-33 0.20758084806946187 1106 34-35 0.22670063122051687 1106 36-37 0.24151035264491583 1106 38-39 0.3335067460383101 1106 40-41 0.4562290193862637 1106 42-43 0.4186359883489317 1106 44-45 0.5755514685064398 1106 46-47 0.4355214800123264 1106 48-49 0.41695462253046855 1106 50-51 0.46094888594497263 1106 52-53 0.33093297709049807 1106 54-55 0.3253301866907279 1106 56-57 0.5272554616657672 1106 58-59 0.5649355870125774 1106 60-61 0.6046312872013502 1106 62-63 0.5275777091985034 1106 64-65 0.4810766846533525 1106 66-67 0.5148330003995625 1106 68-69 0.6634747236222154 1106 70-71 0.5765321351411217 1106 72-73 0.5491662404137969 1106 74-75 0.5273936781488864 1106 76-77 0.5553093893618808 1106 78-79 0.6444304020282949 1106 80-81 0.684975084947208 1106 82-83 0.6084774876952359 1106 84-85 0.7488842547483934 1106 86-87 0.6200403857250407 1106 88-89 0.8092016691938255 1106 90-91 0.8281860865494046 1106 92-93 0.7966597892612022 1106 94-95 0.9198328701137299 1106 96-97 1.0974878031448831 1106 98-99 1.136577748185946 1106 100-101 1.5070827243454978 1107 1 0.10163049927878376 1107 2 0.14114726832746172 1107 3 0.1941556212303155 1107 4 0.16778899138815007 1107 5 0.15522666317423983 1107 6 0.012898603892267602 1107 7 0.0878018433947716 1107 8 0.09756595896685383 1107 9 0.12234903278295661 1107 10-11 0.20144949538103418 1107 12-13 0.152491802105871 1107 14-15 0.2888507570665837 1107 16-17 0.29550217377180843 1107 18-19 0.4093315024294384 1107 20-21 0.24807728575482457 1107 22-23 0.24146378445615113 1107 24-25 0.07628755845624369 1107 26-27 0.1506950142743051 1107 28-29 0.06511509045218133 1107 30-31 0.16276723556462258 1107 32-33 0.13740510284075924 1107 34-35 0.30153227991649345 1107 36-37 0.3668449394907114 1107 38-39 0.26014785184400324 1107 40-41 0.2847041742048688 1107 42-43 0.3616959202275183 1107 44-45 0.5110240929960526 1107 46-47 0.7272471733792969 1107 48-49 0.5734661414760467 1107 50-51 0.5547922426763918 1107 52-53 0.4487167457802457 1107 54-55 0.48419071278164694 1107 56-57 0.7190824254304218 1107 58-59 0.7274038454683662 1107 60-61 0.642530721648896 1107 62-63 0.594303024385745 1107 64-65 0.4827387334389428 1107 66-67 0.44101691758106654 1107 68-69 0.5161017644128876 1107 70-71 0.6214659168933991 1107 72-73 0.5762085438022453 1107 74-75 0.5557759642923372 1107 76-77 0.4491137629184081 1107 78-79 0.3439624199503335 1107 80-81 0.2820238725847801 1107 82-83 0.11348540493888137 1107 84-85 0.4640412279532349 1107 86-87 0.5109735574836414 1107 88-89 0.5984598931718921 1107 90-91 0.7341293138002563 1107 92-93 0.8661460208617378 1107 94-95 0.8668913882134106 1107 96-97 0.9283586754153959 1107 98-99 1.1266175163392838 1107 100-101 1.472296731141288 1108 1 0.2779344648315387 1108 2 0.2878063671197566 1108 3 0.2252660927397514 1108 4 0.19551069220081985 1108 5 0.1942642818164373 1108 6 0.2175264127443981 1108 7 0.28778963281732217 1108 8 0.27918219661957977 1108 9 0.2841814513293457 1108 10-11 0.37572345243334127 1108 12-13 0.4618477880731007 1108 14-15 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-1.3256045635876745 2316 78-79 -1.175744090275959 2316 80-81 -1.0693134118645808 2316 82-83 -1.157451499347733 2316 84-85 -1.2933810911732202 2316 86-87 -1.2575217005086792 2316 88-89 -1.3068239193451596 2316 90-91 -1.6752748527980472 2316 92-93 -1.4199316123091634 2316 94-95 -1.5106118613394912 2316 96-97 -1.7371353265085077 2316 98-99 -1.8652347500833386 2316 100-101 -1.9353984957379673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18476.0 3 4375.0 4 3958.0 5 4414.0 6 4869.0 7 5778.0 8 6790.0 9 8100.0 10 9477.0 11 11071.0 12 13443.0 13 16235.0 14 17604.0 15 18994.0 16 21984.0 17 25738.0 18 23694.0 19 19456.0 20 19649.0 21 21228.0 22 22882.0 23 25556.0 24 28849.0 25 33195.0 26 37696.0 27 43831.0 28 52396.0 29 62282.0 30 74335.0 31 91378.0 32 113826.0 33 145685.0 34 193925.0 35 273566.0 36 398003.0 37 568863.0 38 485770.0 39 76193.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8489120612166 18.47724957912247 14.237661686908515 23.436176672752406 2 15.745840232236679 19.820920309537744 39.820987005884284 24.61225245234129 3 19.7092181004344 23.65922739021794 29.42983411482039 27.201720394527268 4 13.060236046120766 16.13303071694207 36.65801190901767 34.148721327919496 5 14.080821846498424 36.685137701867745 34.195919331652405 15.03812111998142 6 32.69014784059861 35.251815359531086 17.304465038922363 14.753571760947947 7 28.813049705600076 32.121461494411676 20.548093775487185 18.517395024501067 8 26.45535153203417 34.42577377628994 19.684085219765716 19.43478947191018 9 26.222728142669567 16.811674225464916 20.28025236450012 36.6853452673654 10-11 25.30502898334125 26.049945556154558 27.144577099424072 21.500448361080124 12-13 26.159802348471416 23.41513710314935 27.502784499815093 22.922276048564147 14-15 22.97584153106244 25.04856606301899 25.89138944129601 26.08420296462256 16-17 22.604934759862473 28.07253971465411 26.596248316496627 22.726277208986787 18-19 22.384797551281636 26.708545321010547 28.776111314844705 22.13054581286311 20-21 23.085689829496992 25.88473058536375 28.855315121838615 22.174264463300638 22-23 22.690096028970384 25.813252695407336 28.849773205833124 22.64687806978916 24-25 22.720896118058644 25.975373972052374 28.949881671732157 22.35384823815683 26-27 22.43591595838907 26.166188171821208 28.929877970870077 22.46801789891964 28-29 22.252968221805336 26.512396777013304 28.804754832100627 22.429880169080736 30-31 22.525554139753112 26.15208767610283 28.662620917977517 22.659737266166537 32-33 22.293511178060093 26.27975720912577 28.73701913727651 22.689712475537622 34-35 22.891656231499017 26.270278415173117 28.479037006645985 22.35902834668188 36-37 22.82455332635356 26.089617986766278 28.52598323349691 22.559845453383257 38-39 22.520850504427074 26.361951423759006 28.562329796275986 22.55486827553793 40-41 22.75763494241079 26.25402386275459 28.59443638864644 22.39390480618819 42-43 22.51271313091789 26.3798907565426 28.272579685020766 22.83481642751874 44-45 22.633393969804075 26.394993890403544 28.282761447368664 22.688850692423713 46-47 22.865796142210353 26.577709304764046 27.84289119502583 22.71360335799977 48-49 22.821933074194245 26.64983900463315 27.777876198189595 22.750351722983012 50-51 22.618844293578803 26.819175251736425 27.834643919170272 22.727336535514496 52-53 22.882198309789484 26.788800558835668 27.655029270355136 22.673971861019705 54-55 22.6758760275599 26.619811065838196 27.76566161464162 22.93865129196028 56-57 22.70127789762384 26.96922339751558 27.567440141569833 22.76205856329075 58-59 22.8718714996925 26.587788756056064 27.63509579296653 22.905243951284906 60-61 22.637154562022925 26.65164456904418 28.051371232543907 22.659829636388988 62-63 22.56292644651795 26.682778260843413 28.049161526528994 22.705133766109643 64-65 22.41030244692976 26.761020075825254 27.891646402194763 22.93703107505022 66-67 22.428932068402794 26.866518871912493 27.618914853539945 23.085634206144764 68-69 22.538983870207442 26.615494991429784 27.850161124080753 22.995360014282024 70-71 22.589161628781255 26.51991579212007 27.696696508956865 23.194226070141806 72-73 22.56935541724234 26.519561321491096 27.753181662738236 23.157901598528333 74-75 22.505495431170726 26.968544339171057 27.479211626246503 23.04674860341171 76-77 22.631394247078504 26.650835799595264 27.44216748076984 23.275602472556393 78-79 22.610514068889795 26.763903075672385 27.5235071800439 23.10207567539392 80-81 22.70813008116497 26.826362232999962 27.345228573371532 23.120279112463535 82-83 22.679566523114598 26.752469271547348 27.523813757069266 23.044150448268784 84-85 22.622447758719915 26.745603698180652 27.430824544294552 23.20112399880488 86-87 22.665517726528854 26.7234686897103 27.47038003584506 23.140633547915783 88-89 22.623426669251284 26.691426642513854 27.210901519354557 23.4742451688803 90-91 22.60029861977359 26.695614922564616 27.314817213643177 23.38926924401862 92-93 22.81396511062343 26.54694788990642 27.384082327830438 23.25500467163972 94-95 22.63581759680076 26.435251838847073 27.479829640762254 23.449100923589913 96-97 22.635175738615025 26.240898478682144 27.581401980717036 23.542523801985798 98-99 22.590379973656585 26.33271597921946 27.292228024310827 23.784676022813127 100-101 22.904382840286917 25.74256085569962 27.682333147732113 23.670723156281355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3219.0 1 2143.0 2 1139.0 3 1240.5 4 1126.5 5 1063.5 6 1539.0 7 2734.0 8 4186.5 9 4709.5 10 3882.5 11 2993.5 12 2903.0 13 3037.0 14 3190.0 15 3363.0 16 3514.0 17 3811.5 18 4158.0 19 4507.0 20 5096.0 21 5917.0 22 7029.0 23 8525.0 24 10261.5 25 12638.5 26 16193.0 27 20338.0 28 24052.0 29 28191.0 30 32862.0 31 38536.5 32 45214.0 33 49443.5 34 52699.0 35 57528.5 36 63248.0 37 69209.5 38 73945.5 39 76802.5 40 79893.0 41 85013.0 42 88683.0 43 92466.5 44 97980.0 45 102431.5 46 104831.0 47 108938.5 48 117795.0 49 119121.5 50 113525.5 51 110600.0 52 108381.0 53 107661.0 54 106597.5 55 100910.5 56 92483.5 57 84031.0 58 77177.5 59 69548.5 60 58794.0 61 48573.5 62 40351.5 63 31616.5 64 23788.5 65 18449.5 66 13987.5 67 11037.5 68 8503.5 69 6143.5 70 4681.5 71 3522.5 72 2654.5 73 2098.5 74 1649.0 75 1319.0 76 1049.0 77 723.5 78 492.0 79 370.0 80 269.5 81 204.5 82 166.0 83 117.0 84 91.5 85 71.5 86 54.0 87 42.0 88 31.5 89 24.0 90 15.5 91 11.5 92 9.5 93 7.0 94 4.5 95 6.0 96 6.5 97 4.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.268578243264569 2 0.164103404121862 3 0.18900690306346332 4 0.16173956932927686 5 0.22739424835713481 6 0.18987253270581844 7 0.23272120000239713 8 0.23814803199100812 9 0.14782290815602908 10-11 0.2000936211751655 12-13 0.20633614263445726 14-15 0.169829877140519 16-17 0.2780668758826925 18-19 0.22509700046011547 20-21 0.19366798575306782 22-23 0.23819797216268246 24-25 0.22792694352166107 26-27 0.3331175917917002 28-29 0.305184389101856 30-31 0.280780291876998 32-33 0.18720905688318729 34-35 0.3499141361981679 36-37 0.38675333616993507 38-39 0.3675763102469908 40-41 0.32930549202055936 42-43 0.36358109651304404 44-45 0.37190445845876635 46-47 0.377431170790726 48-49 0.39595897448190404 50-51 0.3887010028652341 52-53 0.3881683077007079 54-55 0.3826582420926397 56-57 0.3617666036088765 58-59 0.3364968767416635 60-61 0.3035197167127115 62-63 0.37062266071912514 64-65 0.40320029937468244 66-67 0.4089600658411224 68-69 0.41353791491126957 70-71 0.41367108870240116 72-73 0.40468185780102106 74-75 0.4001206554547652 76-77 0.38450602844459003 78-79 0.3590698343384625 80-81 0.3698402646962272 82-83 0.3800779998894657 84-85 0.3802111736805973 86-87 0.37525044996094675 88-89 0.38400662672784674 90-91 0.4049981455549585 92-93 0.4041491626364948 94-95 0.41235599751497704 96-97 0.41298857302285197 98-99 0.41064138495415825 100-101 0.38340734466775467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3003594.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.04024951189951 #Duplication Level Percentage of deduplicated Percentage of total 1 77.74283617124341 45.12213609141694 2 12.9320582378741 15.011597736572565 3 4.0202764435457885 7.0001354367062865 4 1.7586828417256384 4.082975637842102 5 0.8960644799156392 2.6003902996527084 6 0.561108228408871 1.9540116948018467 7 0.38983353813070115 1.5838225074848742 8 0.2711456113701099 1.2589887150382173 9 0.21405323483095814 1.1181332842576226 >10 1.1067075111128766 11.648817468731117 >50 0.07190696145059484 2.8600974449746546 >100 0.03205479119278276 3.3061572557937584 >500 0.0023535073183894545 0.9663153170787944 >1k 7.462340276155967E-4 0.7069787279332426 >5k 1.7220785252667617E-4 0.7794423817152785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9901 0.32963842649838826 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8352 0.2780668758826925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5142 0.17119490849961747 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3658 0.12178743198980956 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.329344778288943E-5 9.988034334866829E-5 2 0.0 0.0 0.0 3.329344778288943E-5 9.988034334866829E-5 3 0.0 0.0 0.0 3.329344778288943E-5 9.988034334866829E-5 4 0.0 3.329344778288943E-5 0.0 3.329344778288943E-5 9.988034334866829E-5 5 0.0 6.658689556577886E-5 0.0 3.329344778288943E-5 9.988034334866829E-5 6 0.0 6.658689556577886E-5 0.0 3.329344778288943E-5 1.3317379113155772E-4 7 0.0 6.658689556577886E-5 0.0 6.658689556577886E-5 1.3317379113155772E-4 8 0.0 6.658689556577886E-5 0.0 6.658689556577886E-5 1.3317379113155772E-4 9 0.0 6.658689556577886E-5 0.0 9.988034334866829E-5 1.3317379113155772E-4 10-11 0.0 6.658689556577886E-5 0.0 9.988034334866829E-5 1.3317379113155772E-4 12-13 0.0 9.988034334866829E-5 0.0 1.16527067240113E-4 2.8299430615456015E-4 14-15 0.0 9.988034334866829E-5 0.0 1.3317379113155772E-4 3.6622792561178373E-4 16-17 0.0 9.988034334866829E-5 0.0 1.4982051502300243E-4 4.328148211775626E-4 18-19 0.0 9.988034334866829E-5 0.0 2.33054134480226E-4 5.326951645262309E-4 20-21 0.0 1.3317379113155772E-4 0.0 2.9964103004600487E-4 5.659886123091203E-4 22-23 0.0 1.3317379113155772E-4 0.0 3.9952137339467316E-4 6.492222317663438E-4 24-25 0.0 1.4982051502300243E-4 0.0 6.159287839834545E-4 6.658689556577886E-4 26-27 0.0 1.6646723891444715E-4 0.0 8.989230901380146E-4 6.825156795492334E-4 28-29 0.0 1.6646723891444715E-4 0.0 0.002463715135933818 6.99162403440678E-4 30-31 0.0 1.6646723891444715E-4 0.0 0.006309108354857548 6.99162403440678E-4 32-33 0.0 1.6646723891444715E-4 0.0 0.015131872017323248 7.324558512235675E-4 34-35 0.0 1.6646723891444715E-4 0.0 0.029697755422337373 8.323361945722357E-4 36-37 0.0 1.6646723891444715E-4 0.0 0.05588305210357991 8.323361945722357E-4 38-39 0.0 1.6646723891444715E-4 0.0 0.09628465098811623 8.323361945722357E-4 40-41 0.0 1.6646723891444715E-4 0.0 0.13885032397854036 8.489829184636804E-4 42-43 0.0 1.9976068669733658E-4 0.0 0.18306402263421753 8.656296423551252E-4 44-45 0.0 1.9976068669733658E-4 0.0 0.24066168729861626 8.656296423551252E-4 46-47 0.0 1.9976068669733658E-4 0.0 0.3191343437228866 8.989230901380147E-4 48-49 0.0 1.9976068669733658E-4 0.0 0.401235985955492 9.655099857037936E-4 50-51 0.0 1.9976068669733658E-4 0.0 0.48976326361019495 0.0010320968812695724 52-53 0.0 1.9976068669733658E-4 0.0 0.5759932933678786 0.001181917396292575 54-55 0.0 1.9976068669733658E-4 0.0 0.678270764956915 0.0011985641201840195 56-57 0.0 1.9976068669733658E-4 0.0 0.8040034705089969 0.0011985641201840195 58-59 0.0 1.9976068669733658E-4 0.0 0.952725301755164 0.0011985641201840195 60-61 0.0 1.9976068669733658E-4 0.0 1.105675400869758 0.0012152108440754642 62-63 0.0 1.9976068669733658E-4 0.0 1.2604899330601937 0.0012651510157497983 64-65 0.0 1.9976068669733658E-4 0.0 1.4256920209588912 0.0013317379113155772 66-67 0.0 1.9976068669733658E-4 0.0 1.6219568956390245 0.0013317379113155772 68-69 0.0 1.9976068669733658E-4 0.0 1.8446567678587718 0.0013317379113155772 70-71 0.0 1.9976068669733658E-4 0.0 2.088414745801197 0.001348384635207022 72-73 0.0 1.9976068669733658E-4 0.0 2.3341536838867034 0.0014316182546642454 74-75 0.0 1.9976068669733658E-4 0.0 2.5866178984243544 0.0014815584263385797 76-77 0.0 1.9976068669733658E-4 0.0 2.8640355520752805 0.0015314985980129138 78-79 0.0 1.9976068669733658E-4 0.0 3.1883303802045146 0.0015314985980129138 80-81 0.0 2.164074105887813E-4 0.0 3.5348652314527196 0.0015481453219043585 82-83 0.0 2.3305413448022604E-4 0.0 3.8909386554907224 0.0016147322174701374 84-85 3.329344778288943E-5 2.3305413448022604E-4 0.0 4.257149934378614 0.0016313789413615822 86-87 3.329344778288943E-5 2.3305413448022604E-4 0.0 4.639025780448357 0.0016480256652530267 88-89 3.329344778288943E-5 2.3305413448022604E-4 0.0 5.065048738278209 0.0016646723891444715 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9780 0.0 29.049427 1 GTATCAA 13740 0.0 28.21491 1 GTACATG 10120 0.0 23.989191 1 TATCAAC 16595 0.0 23.412264 2 TCAACGC 16585 0.0 23.369173 4 ATCAACG 16570 0.0 23.361698 3 TACATGG 10280 0.0 23.350393 2 CAACGCA 16770 0.0 23.083086 5 ACATGGG 10240 0.0 22.654041 3 AACGCAG 17195 0.0 22.566978 6 GTGTACG 345 0.0 22.033127 1 GAGTACT 9545 0.0 21.781185 12-13 CATGGGG 6595 0.0 20.572544 4 TACCTGG 2285 0.0 20.345888 2 ACGCAGA 18940 0.0 20.28778 7 AGAGTAC 14920 0.0 19.929468 10-11 GTACTTT 10490 0.0 19.864592 14-15 CGCAGAG 19425 0.0 19.707977 8 AGTACTT 10375 0.0 19.604063 12-13 GTACCTG 2475 0.0 19.579437 1 >>END_MODULE