FastQCFastQC Report
Thu 26 May 2016
SRR938678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003594
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95470.3178525459832454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90560.3015054631218467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57050.1899391196013842No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41970.13973260034478696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111550.027.7616961
GTATCAA151700.026.3721181
TATCAAC181950.021.8849412
TCAACGC182150.021.8609094
ATCAACG182300.021.8429223
CAACGCA185050.021.5439665
AACGCAG189250.021.0863556
GAGTACT109250.020.67411612-13
GTACATG100250.019.834741
GTACTTT115550.019.341614-15
TACATGG99750.019.1746562
AGAGTAC164700.018.87074910-11
ACGCAGA210900.018.8301687
CGCAGAG211850.018.6561418
ACATGGG100900.018.1564773
AGTACTT118050.017.50506212-13
GCAGAGT226300.017.2342579
TACCTGG22600.017.0103022
ACTTTTT135400.016.61051416-17
TATAGCG2355.175195E-516.1568835
Produced by FastQC (version 0.11.5)