##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938678_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3003594 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.973030309688994 31.0 31.0 34.0 30.0 34.0 2 32.19201696367752 33.0 31.0 34.0 30.0 34.0 3 32.2711005548686 34.0 31.0 34.0 30.0 34.0 4 35.90890146937303 37.0 35.0 37.0 35.0 37.0 5 35.80818779102635 37.0 35.0 37.0 35.0 37.0 6 35.834489281840355 37.0 35.0 37.0 35.0 37.0 7 35.75254445174681 37.0 35.0 37.0 33.0 37.0 8 35.72914048969335 37.0 35.0 37.0 33.0 37.0 9 37.365938605550554 39.0 37.0 39.0 34.0 39.0 10-11 37.28481645655172 39.0 37.0 39.0 33.5 39.0 12-13 37.198474061407765 39.0 37.0 39.0 33.0 39.0 14-15 38.38994001852447 40.0 38.0 41.0 33.0 41.0 16-17 38.29814698657675 40.0 38.0 41.0 33.0 41.0 18-19 38.44240366707351 40.0 38.0 41.0 33.5 41.0 20-21 38.442231207014 40.0 38.0 41.0 34.0 41.0 22-23 38.367110701379744 40.0 38.0 41.0 34.0 41.0 24-25 38.282565486547114 40.0 38.0 41.0 33.0 41.0 26-27 38.09406148101242 40.0 37.5 41.0 33.0 41.0 28-29 37.91323760801227 40.0 37.0 41.0 33.0 41.0 30-31 37.682231353505166 40.0 37.0 41.0 32.0 41.0 32-33 37.524438389476074 40.0 36.5 41.0 32.0 41.0 34-35 37.49769392933932 40.0 36.5 41.0 32.0 41.0 36-37 37.38200752165572 40.0 36.0 41.0 31.5 41.0 38-39 37.248412401942474 40.0 36.0 41.0 31.0 41.0 40-41 37.06144788543325 40.0 36.0 41.0 31.0 41.0 42-43 36.85773809642715 39.0 36.0 41.0 30.5 41.0 44-45 36.61037327281916 39.0 35.0 41.0 30.0 41.0 46-47 36.30818462814881 39.0 35.0 41.0 30.0 41.0 48-49 36.00227893650074 39.0 35.0 40.5 29.0 41.0 50-51 35.75286073950075 39.0 35.0 40.0 28.0 41.0 52-53 35.50908261902241 38.5 35.0 40.0 27.5 41.0 54-55 35.212608628196755 38.0 34.0 40.0 27.0 41.0 56-57 34.91289701604145 38.0 34.0 40.0 26.0 41.0 58-59 34.57699642494958 38.0 34.0 40.0 26.0 41.0 60-61 34.18316473531377 37.0 33.0 40.0 25.5 41.0 62-63 33.800562426213396 36.5 33.0 39.5 24.5 41.0 64-65 33.37527109189857 36.0 32.5 39.0 23.5 40.5 66-67 32.94316608702774 35.5 32.0 39.0 22.5 40.0 68-69 32.5666263483014 35.0 31.5 38.5 22.0 40.0 70-71 32.10477031849178 35.0 31.0 38.0 20.5 40.0 72-73 31.59217923594201 35.0 31.0 37.0 19.5 39.0 74-75 31.08007240659024 34.5 30.0 36.5 18.0 39.0 76-77 29.199735883078738 32.5 28.0 34.5 13.0 37.0 78-79 30.16604058338111 34.0 29.5 35.0 13.0 37.5 80-81 30.216786622959027 34.0 30.0 35.0 12.5 37.0 82-83 29.8678141919314 34.0 30.0 35.0 7.5 37.0 84-85 29.437408817569885 34.0 29.0 35.0 4.5 36.0 86-87 28.80433057863346 34.0 29.0 35.0 2.0 36.0 88-89 28.15099693900041 33.0 27.5 35.0 2.0 35.0 90-91 27.580695160531015 33.0 26.5 35.0 2.0 35.0 92-93 27.226434731192033 33.0 26.0 35.0 2.0 35.0 94-95 26.804575784876384 33.0 25.0 35.0 2.0 35.0 96-97 26.086475901869562 32.0 24.5 34.0 2.0 35.0 98-99 25.101830340585312 32.0 20.5 34.0 2.0 35.0 100-101 21.27493878999625 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09127358394028207 1101 2 0.11640095954743401 1101 3 0.017313125959496745 1101 4 0.0565472383333514 1101 5 -0.13575921878893382 1101 6 0.04780445654925103 1101 7 0.10100143336561729 1101 8 0.179166132935336 1101 9 0.18081806318415516 1101 10-11 0.1835166442834648 1101 12-13 0.17618866026386115 1101 14-15 0.37748350101278305 1101 16-17 0.38064375176746523 1101 18-19 0.36328822212858825 1101 20-21 0.3293590295785265 1101 22-23 0.2351759497654129 1101 24-25 0.2546199704508183 1101 26-27 0.20442717442364966 1101 28-29 0.21739074647260992 1101 30-31 0.24757619059393932 1101 32-33 0.2751696496798317 1101 34-35 0.5224928649795118 1101 36-37 0.4195042191421763 1101 38-39 0.3993581452985282 1101 40-41 0.3945649925840371 1101 42-43 0.46226499818754263 1101 44-45 0.5099835357030074 1101 46-47 0.5996202918093374 1101 48-49 0.5247532874191023 1101 50-51 0.4166603498749808 1101 52-53 0.3879400759955658 1101 54-55 0.44642410790739007 1101 56-57 0.4900125636457844 1101 58-59 0.5636528351449286 1101 60-61 0.590698203188353 1101 62-63 0.3760146137567304 1101 64-65 0.38499569633227537 1101 66-67 0.43135063287844133 1101 68-69 0.3116013472822061 1101 70-71 0.4005883536554933 1101 72-73 0.3336272234291471 1101 74-75 0.22465829055057895 1101 76-77 0.32142405481306824 1101 78-79 0.10885374899731914 1101 80-81 0.3255923004949679 1101 82-83 0.44574379180777157 1101 84-85 0.49220611727522723 1101 86-87 0.4437590780840672 1101 88-89 0.5546380414978849 1101 90-91 0.4614862791310017 1101 92-93 0.5841662599661817 1101 94-95 0.6133920583142327 1101 96-97 0.6915995008087883 1101 98-99 0.3946558570286811 1101 100-101 1.0323082522919762 1102 1 0.26415610050836236 1102 2 0.15903670343550402 1102 3 0.1843484019823478 1102 4 0.18755970829115398 1102 5 0.09542160785031939 1102 6 0.06830655192687374 1102 7 0.17561172837775985 1102 8 0.21875853910974286 1102 9 0.2885046536701239 1102 10-11 0.322327351450312 1102 12-13 0.3212695415389817 1102 14-15 0.3899110633636482 1102 16-17 0.3306641918547939 1102 18-19 0.35558423285114316 1102 20-21 0.37190643618065167 1102 22-23 0.329515012771175 1102 24-25 0.4389375444983088 1102 26-27 0.2562495661846853 1102 28-29 0.34756368981509667 1102 30-31 0.36874576981185214 1102 32-33 0.42054584830503217 1102 34-35 0.4704337249935051 1102 36-37 0.46638477380576404 1102 38-39 0.5252969170276742 1102 40-41 0.49209472957964806 1102 42-43 0.561144457984625 1102 44-45 0.6032883431857599 1102 46-47 0.4498842934840894 1102 48-49 0.5224685004753056 1102 50-51 0.5487168547876919 1102 52-53 0.41996809159623893 1102 54-55 0.46014997044253647 1102 56-57 0.403356372792544 1102 58-59 0.4932911122282633 1102 60-61 0.6164412680666018 1102 62-63 0.5588953635790901 1102 64-65 0.6060946809478338 1102 66-67 0.5887372097376797 1102 68-69 0.5725106259642132 1102 70-71 0.6007243737319143 1102 72-73 0.6352332318261311 1102 74-75 0.5012901553356421 1102 76-77 0.4454512162717066 1102 78-79 0.5303319498913517 1102 80-81 0.8313433049823331 1102 82-83 0.7674845758216051 1102 84-85 0.9019751679309636 1102 86-87 1.0210679589688674 1102 88-89 1.0338111292750547 1102 90-91 1.0653267587573296 1102 92-93 0.9897915718870713 1102 94-95 1.083856508252353 1102 96-97 1.0428095281869005 1102 98-99 1.3513778408906845 1102 100-101 2.0261399562215026 1103 1 0.15688154526042908 1103 2 0.12200822200972539 1103 3 0.09112848685526842 1103 4 -0.013295887370595949 1103 5 0.035839326386224 1103 6 -0.004928978054159927 1103 7 0.040541546306187115 1103 8 0.06311735522915995 1103 9 -0.033129124598950455 1103 10-11 0.09852652476162405 1103 12-13 0.0976929346586104 1103 14-15 0.1807576393703414 1103 16-17 1.4957136695414874E-4 1103 18-19 -0.08784349885819864 1103 20-21 0.03246625666917424 1103 22-23 0.07799209826970355 1103 24-25 0.09663103898733993 1103 26-27 0.13468555460833187 1103 28-29 0.07473735038784213 1103 30-31 0.09336548623525687 1103 32-33 0.13895480319489195 1103 34-35 0.05289087739345888 1103 36-37 0.12082941228204902 1103 38-39 0.07169091997432275 1103 40-41 0.2353927189764704 1103 42-43 0.1850225112311037 1103 44-45 0.21976565926193103 1103 46-47 0.12101847272091959 1103 48-49 0.11163868373368047 1103 50-51 0.20479821309348978 1103 52-53 0.15683533821089668 1103 54-55 0.1336410008024913 1103 56-57 -2.8124391619854805E-4 1103 58-59 0.1537104244626093 1103 60-61 0.24749393132321273 1103 62-63 0.2812845379585198 1103 64-65 0.20675644727434417 1103 66-67 0.22763120844576434 1103 68-69 0.23077463058932324 1103 70-71 0.25365118397837705 1103 72-73 0.3831959681046442 1103 74-75 0.3869969690746764 1103 76-77 0.3426869124042753 1103 78-79 0.1468724205071119 1103 80-81 0.32179976395392274 1103 82-83 0.39230083425845663 1103 84-85 0.33300002623591496 1103 86-87 0.37245494286064584 1103 88-89 0.3497980863791348 1103 90-91 0.5032199911338182 1103 92-93 0.5905215790248057 1103 94-95 0.5033609612831782 1103 96-97 0.6354322302517552 1103 98-99 0.837002977584234 1103 100-101 1.4333044784595295 1104 1 0.19186262972844403 1104 2 0.19801231376902706 1104 3 0.20007832485465826 1104 4 0.12930302070649446 1104 5 0.06184502044204265 1104 6 0.11060714192580434 1104 7 0.09877912134005129 1104 8 0.11807612560994585 1104 9 0.1893830570711117 1104 10-11 0.16755345852268277 1104 12-13 0.11052269149746508 1104 14-15 0.09509528372461062 1104 16-17 0.329423612982815 1104 18-19 0.33386976933756785 1104 20-21 0.3329194114223455 1104 22-23 0.266976436242949 1104 24-25 0.2779876022468457 1104 26-27 0.20263030573794794 1104 28-29 0.21221101790963104 1104 30-31 0.058875632145436896 1104 32-33 0.21209963899323014 1104 34-35 0.19669066454101625 1104 36-37 0.28095786586352034 1104 38-39 0.2788364456034742 1104 40-41 0.2522339094659074 1104 42-43 0.27453543839070704 1104 44-45 0.2866762966554859 1104 46-47 0.2713231620524894 1104 48-49 0.3157807467157383 1104 50-51 0.16551728160262513 1104 52-53 0.1110210426900764 1104 54-55 0.18333960039533537 1104 56-57 0.2664631762970231 1104 58-59 0.43554130965554094 1104 60-61 0.5853893823190859 1104 62-63 0.4261580745979856 1104 64-65 0.4233032368624521 1104 66-67 0.3865568855003758 1104 68-69 0.4246961501061435 1104 70-71 0.5390530821094259 1104 72-73 0.559022710939427 1104 74-75 0.5458324115362068 1104 76-77 0.5626023195815542 1104 78-79 0.40385885495885177 1104 80-81 0.4752434786506896 1104 82-83 0.568674466192391 1104 84-85 0.5516719669880494 1104 86-87 0.67921735096051 1104 88-89 0.63107175187697 1104 90-91 0.6455534661438946 1104 92-93 0.9138158922214892 1104 94-95 1.0969211629364075 1104 96-97 1.0660958002695828 1104 98-99 1.3732863496256797 1104 100-101 2.094517433785814 1105 1 0.1590767871663381 1105 2 0.13568815932424627 1105 3 0.11180385613271682 1105 4 0.02460881737415832 1105 5 -0.08896561509622103 1105 6 -0.009985495009246392 1105 7 0.09465236288449574 1105 8 0.13882590112626048 1105 9 0.14595793485221975 1105 10-11 0.12619795118955324 1105 12-13 0.2020577857806103 1105 14-15 0.21368876870914733 1105 16-17 0.2117349219721376 1105 18-19 0.23117908291634137 1105 20-21 0.12831126017022854 1105 22-23 0.15132618182810376 1105 24-25 0.07977848322060765 1105 26-27 0.1777076278969858 1105 28-29 0.21410541080597767 1105 30-31 0.1603472474302805 1105 32-33 0.1348466957626684 1105 34-35 0.23434343816168735 1105 36-37 0.13127775511823359 1105 38-39 0.11571412693641037 1105 40-41 0.04001139624631378 1105 42-43 0.147864905616089 1105 44-45 0.1370535539636748 1105 46-47 0.035751851067750806 1105 48-49 0.02859627100985307 1105 50-51 0.10703117464861833 1105 52-53 0.11770005595955269 1105 54-55 0.1570344771787333 1105 56-57 0.16062223546094856 1105 58-59 0.2184269227454294 1105 60-61 0.2587543927949838 1105 62-63 0.17041752785548425 1105 64-65 0.11070408990046587 1105 66-67 0.08658868735611236 1105 68-69 0.10274476329580295 1105 70-71 0.15861496477928938 1105 72-73 0.0868412283493818 1105 74-75 0.2557202944056094 1105 76-77 0.37007637924432757 1105 78-79 0.07908520934376284 1105 80-81 0.23140306160988544 1105 82-83 0.33738081658648866 1105 84-85 0.3468985707992829 1105 86-87 0.5751965986907273 1105 88-89 0.37182177681960127 1105 90-91 0.38992204341617054 1105 92-93 0.6018895727562601 1105 94-95 0.7474212627069399 1105 96-97 0.8110461644320104 1105 98-99 1.1423518739198038 1105 100-101 1.713911577255832 1106 1 0.17593551763476611 1106 2 0.1570802052407103 1106 3 0.08059033809294647 1106 4 0.0991190038691272 1106 5 -0.0019808338660354252 1106 6 0.07237146318792043 1106 7 0.039795079499945984 1106 8 0.12163202440479637 1106 9 0.09178171580599326 1106 10-11 0.10584132224461484 1106 12-13 0.16230230637310683 1106 14-15 0.18213482012127713 1106 16-17 0.20938899982199644 1106 18-19 -0.018607207996303998 1106 20-21 0.05194974121992857 1106 22-23 0.19980410160437856 1106 24-25 0.24607159087389618 1106 26-27 0.2506987827999936 1106 28-29 0.3105318046404193 1106 30-31 0.22412787601750495 1106 32-33 0.22333719622605486 1106 34-35 0.30365682531353144 1106 36-37 0.1479069236140802 1106 38-39 0.21929200118644587 1106 40-41 0.32671255310044955 1106 42-43 0.20347184755387815 1106 44-45 0.18130210687979798 1106 46-47 0.11333353407508184 1106 48-49 0.14698195951286408 1106 50-51 0.2957895482238868 1106 52-53 0.18803697041901302 1106 54-55 0.294194961220164 1106 56-57 0.24456275462261345 1106 58-59 0.34239872086909173 1106 60-61 0.3890771006303737 1106 62-63 0.4723363366940774 1106 64-65 0.5976792171300289 1106 66-67 0.5497970877593445 1106 68-69 0.4090089902440823 1106 70-71 0.4560924252283627 1106 72-73 0.3698587234309265 1106 74-75 0.552310196524207 1106 76-77 0.5697831008676637 1106 78-79 0.3341188052659163 1106 80-81 0.31744784804306647 1106 82-83 0.4785020830287756 1106 84-85 0.5208160016563141 1106 86-87 0.6235373300371663 1106 88-89 0.753885863124335 1106 90-91 0.591901113542832 1106 92-93 0.8876083588317378 1106 94-95 1.0686785590989842 1106 96-97 1.1586164049080843 1106 98-99 1.3899134766058516 1106 100-101 1.8111619146377507 1107 1 0.02256994294672765 1107 2 0.05029288084386252 1107 3 -6.657664432907495E-4 1107 4 0.020398134440071658 1107 5 -0.05155426757848858 1107 6 -0.0696393163651905 1107 7 -0.014192427077318825 1107 8 -0.0010825946333028469 1107 9 0.04649356943040317 1107 10-11 -0.0907523414256346 1107 12-13 -0.023166945162202524 1107 14-15 -0.038042744458536504 1107 16-17 -0.02296012588780627 1107 18-19 0.02690733516054422 1107 20-21 -0.10411756986498943 1107 22-23 -0.0293370379308584 1107 24-25 0.029774763536039472 1107 26-27 0.0641295393188912 1107 28-29 -0.0018588554242526811 1107 30-31 0.029635051824463687 1107 32-33 -0.02920100299247963 1107 34-35 -0.013903040536916933 1107 36-37 -0.0732494109780788 1107 38-39 -0.027327583126577792 1107 40-41 -0.05001209976122567 1107 42-43 -0.030785654816384067 1107 44-45 0.013252222316602058 1107 46-47 0.039862412492141175 1107 48-49 0.11373117157029355 1107 50-51 -0.024195267349099936 1107 52-53 -0.06292503703256358 1107 54-55 0.06356655325868843 1107 56-57 0.019409060338190898 1107 58-59 0.1808029332597343 1107 60-61 0.17458970996009526 1107 62-63 0.11113381511432863 1107 64-65 0.07839521969265917 1107 66-67 0.05388429068894851 1107 68-69 -0.056926726561854935 1107 70-71 -0.11858181893345687 1107 72-73 -0.1147693950697537 1107 74-75 0.11350652413332796 1107 76-77 0.05838231090132595 1107 78-79 -0.012855164112565376 1107 80-81 0.23962418445866973 1107 82-83 0.1504545779315265 1107 84-85 0.10222112944631334 1107 86-87 0.14463567025746116 1107 88-89 0.07572682567145606 1107 90-91 0.3604841497495528 1107 92-93 0.609381637429177 1107 94-95 0.6904995496681998 1107 96-97 0.843375802878672 1107 98-99 1.0758144662919698 1107 100-101 1.7742417612562313 1108 1 0.18774517479674202 1108 2 0.11292274402501334 1108 3 0.04927750804445452 1108 4 0.10890402268913846 1108 5 0.06289876228844804 1108 6 0.12768093152294568 1108 7 0.07116487655881798 1108 8 0.15572403074551033 1108 9 0.07852016970373654 1108 10-11 0.14840255243291267 1108 12-13 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-0.8685321032862774 2316 50-51 -1.0070198838593214 2316 52-53 -1.0685089812332222 2316 54-55 -1.0047527518504822 2316 56-57 -1.006703733025482 2316 58-59 -0.7718389729987862 2316 60-61 -0.9497671398665091 2316 62-63 -1.14834047741288 2316 64-65 -1.015975959158233 2316 66-67 -1.014708317163091 2316 68-69 -0.9738171702936995 2316 70-71 -0.9195265744072572 2316 72-73 -0.9627545811191887 2316 74-75 -1.2417616710841308 2316 76-77 -1.191009860152466 2316 78-79 -1.0318332215027972 2316 80-81 -0.9732801714310284 2316 82-83 -0.9911745798706519 2316 84-85 -1.2541184003696664 2316 86-87 -1.3595033698923693 2316 88-89 -1.7214226829305126 2316 90-91 -2.37528147165186 2316 92-93 -2.0285192698933905 2316 94-95 -1.9568674465417146 2316 96-97 -2.027000610502135 2316 98-99 -2.1332068333955014 2316 100-101 -2.0840792514253828 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 13.0 4 27.0 5 105.0 6 230.0 7 565.0 8 1260.0 9 2414.0 10 3726.0 11 5631.0 12 7630.0 13 10174.0 14 12904.0 15 16568.0 16 20149.0 17 26097.0 18 25419.0 19 20052.0 20 19848.0 21 20914.0 22 22332.0 23 25109.0 24 28978.0 25 34574.0 26 41494.0 27 50169.0 28 60195.0 29 72928.0 30 87277.0 31 105978.0 32 128992.0 33 162458.0 34 212989.0 35 295040.0 36 430238.0 37 602796.0 38 418148.0 39 30163.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.11069358597071 19.73154998449992 14.979716420306072 23.178040009223295 2 16.453755068095088 20.810635525307347 38.35238717349948 24.38322223309808 3 19.91167914172155 23.924804750575476 29.8383869457723 26.325129161930672 4 13.818312328497125 17.12691528881733 35.86030602005464 33.194466362630905 5 13.941951937211064 37.09170906567223 34.14048518360396 14.825853813512754 6 32.619224795361355 35.9608652006932 16.766101819065646 14.653808184879797 7 28.657168711883163 32.32011383695666 20.377487769651957 18.64522968150822 8 26.493261073234265 34.99937075383691 19.282932380341684 19.224435792587148 9 25.964727589680898 16.752364001259824 20.04232263082161 37.24058577823767 10-11 25.284109636655288 26.342008939956596 26.919567025370274 21.45431439801784 12-13 26.03149759920948 23.58068367429153 27.475600896792308 22.912217829706677 14-15 22.751260656400298 25.300240312106098 25.78168021377057 26.166818817723037 16-17 22.375827049739097 28.322228316917887 26.554670391182356 22.747274242160664 18-19 22.206663084291687 26.943072199504993 28.812782286820386 22.037482429382933 20-21 22.901713746931176 26.019695071970446 28.955378123674503 22.123213057423875 22-23 22.56045590715656 25.96902244444489 28.908550889367874 22.56197075903068 24-25 22.646578221218387 26.132363869297482 28.91815244720973 22.302905462274403 26-27 22.365456360578467 26.330312631563004 28.904182265561285 22.400048742297244 28-29 22.201236252303076 26.642765300503328 28.867033959982606 22.28896448721099 30-31 22.442447281490107 26.282213907738527 28.74215023734899 22.533188573422375 32-33 22.252275107754244 26.37020849022871 28.775377098236316 22.60213930378074 34-35 22.743599672504605 26.41718505121361 28.57477239968955 22.264442876592234 36-37 22.79220626781177 26.169676689680067 28.600360526794677 22.437756515713485 38-39 22.44790896655705 26.44618880369099 28.65736060553995 22.44854162421201 40-41 22.681953938409187 26.325527657265006 28.617486356992313 22.375032047333494 42-43 22.526433306957323 26.42944461712139 28.33559274685806 22.70852932906323 44-45 22.64968391655144 26.466707441603553 28.251787343943473 22.63182129790153 46-47 22.878672410480963 26.717659165948355 27.851706247779735 22.551962175790948 48-49 22.843248773655215 26.783782933435702 27.71067269133634 22.662295601572744 50-51 22.63437866488214 26.883490353634855 27.792934627450293 22.689196354032713 52-53 22.926467425357757 26.8889703468578 27.54763127107059 22.636930956713858 54-55 22.67257159256544 26.780500293981145 27.640553283832638 22.90637482962078 56-57 22.721830705731826 27.02981425726712 27.494212706708666 22.75414233029239 58-59 22.859240826958725 26.755253057680424 27.50848652809444 22.87701958726641 60-61 22.626226103447323 26.7998304655306 27.921681201284226 22.65226222973785 62-63 22.522115798706572 26.830195645808292 27.911310740518225 22.736377814966914 64-65 22.463684639842853 26.863261806861882 27.73656173527992 22.936491818015348 66-67 22.423769123685787 27.012271721900017 27.469968292696734 23.09399086171747 68-69 22.518140821718045 26.7441407518016 27.678041407185077 23.059677019295275 70-71 22.558911517154367 26.6972378252916 27.53568350710574 23.20816715044829 72-73 22.49856338772817 26.71061734708486 27.585902089297022 23.20491717588995 74-75 22.396449438187283 27.099418993528058 27.27449334644213 23.229638221842528 76-77 22.44989263301946 26.819858660681035 27.43709387598464 23.293154830314865 78-79 22.42696124992957 26.886535011490626 27.43512788053348 23.251375858046323 80-81 22.54333518446752 26.918237800160078 27.325836905755963 23.212590109616443 82-83 22.478488063351385 26.897070647713633 27.43689582213137 23.187545466803616 84-85 22.3829185968543 26.906798988145535 27.3627694022561 23.347513012744066 86-87 22.42003503341372 26.814958787747905 27.380035886586658 23.384970292251722 88-89 22.341620616460542 26.845328269856967 27.13455045861445 23.678500655068042 90-91 22.310881203055764 26.842125667671752 27.221272317832813 23.62572081143967 92-93 22.414130057334855 26.722517739646285 27.302674838684332 23.560677364334527 94-95 22.222240718634445 26.627159439942695 27.36900614282497 23.78159369859789 96-97 22.179543693018225 26.462448961110564 27.514689078942812 23.8433182669284 98-99 22.093315541314837 26.4801601015317 27.190825391181367 24.235698965972098 100-101 22.31053790906512 25.842351532649964 27.77717478881699 24.069935769467925 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1246.0 1 850.5 2 617.5 3 811.5 4 686.0 5 616.5 6 1101.5 7 2303.5 8 3565.5 9 4055.5 10 3406.0 11 2531.5 12 2415.5 13 2610.0 14 2807.0 15 2964.0 16 3185.0 17 3516.0 18 3882.0 19 4285.0 20 4860.5 21 5718.0 22 6895.5 23 8413.5 24 10290.0 25 12771.0 26 16217.5 27 20448.5 28 24417.0 29 28642.0 30 33557.5 31 39265.0 32 45884.5 33 50115.0 34 53247.5 35 57823.0 36 63556.0 37 69584.5 38 74415.0 39 77635.5 40 80583.5 41 85497.0 42 89434.0 43 93614.0 44 99229.0 45 103443.5 46 105471.0 47 109894.5 48 119838.5 49 121484.0 50 114853.5 51 110960.5 52 108790.0 53 108458.5 54 107184.5 55 100966.5 56 92472.5 57 83775.5 58 76983.0 59 69307.0 60 58336.0 61 48091.0 62 39686.5 63 31043.0 64 23125.5 65 17593.0 66 13243.0 67 10430.5 68 7854.5 69 5544.0 70 4044.5 71 2913.0 72 2211.0 73 1781.0 74 1424.5 75 1157.0 76 831.0 77 501.5 78 332.0 79 220.0 80 131.5 81 99.5 82 71.5 83 45.5 84 26.0 85 12.0 86 6.5 87 3.0 88 2.5 89 1.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22756071559604926 2 0.0 3 0.0 4 0.0 5 0.025702541688390645 6 0.004294854763992737 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0023804815164765944 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.3293447782889435E-4 26-27 0.0014149715307728008 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006425635422097661 36-37 0.016263849241941488 38-39 0.012984444635326877 40-41 0.007457732303367232 42-43 0.007774020057304683 44-45 0.0038287464950322848 46-47 4.994017167433414E-4 48-49 0.0018144929041674742 50-51 4.994017167433415E-5 52-53 0.0 54-55 0.0 56-57 0.0010320968812695722 58-59 3.3293447782889435E-4 60-61 0.002497008583716707 62-63 9.488632618123488E-4 64-65 0.001464911702447135 66-67 0.006159287839834545 68-69 2.9964103004600487E-4 70-71 1.6646723891444715E-5 72-73 0.0 74-75 4.328148211775626E-4 76-77 0.05793059914222761 78-79 0.1395994265536554 80-81 9.98803433486683E-4 82-83 9.655099857037935E-4 84-85 0.0 86-87 0.10087914678215497 88-89 0.3460354495314613 90-91 0.3822087805475707 92-93 3.6622792561178373E-4 94-95 2.829943061545602E-4 96-97 6.658689556577886E-5 98-99 0.0 100-101 0.0012651510157497983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3003594.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.32637036041609 #Duplication Level Percentage of deduplicated Percentage of total 1 76.31174182228467 42.98363432730461 2 13.776177109369195 15.519241080260313 3 4.231463276267534 7.150289029966345 4 1.8575715629306795 4.185210552984417 5 0.9711273881680391 2.735004046654827 6 0.6133744077239399 2.07294924354357 7 0.412087380904073 1.6247970506359672 8 0.2830028767519304 1.2752419879193937 9 0.22465732415462925 1.138871848006233 >10 1.200146269738919 12.233881017008834 >50 0.08105725914941474 3.1117524919756767 >100 0.03422000927720775 3.4499412352203085 >500 0.002367237389449075 0.9510991688116015 >1k 8.285330861321655E-4 0.7577925627796778 >5k 1.7754280417117834E-4 0.810294356928184 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9547 0.3178525459832454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9056 0.3015054631218467 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5705 0.1899391196013842 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4197 0.13973260034478696 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.329344778288943E-5 2 0.0 0.0 0.0 0.0 3.329344778288943E-5 3 0.0 0.0 0.0 0.0 3.329344778288943E-5 4 0.0 0.0 0.0 3.329344778288943E-5 3.329344778288943E-5 5 0.0 0.0 0.0 3.329344778288943E-5 3.329344778288943E-5 6 0.0 0.0 0.0 3.329344778288943E-5 3.329344778288943E-5 7 0.0 0.0 0.0 3.329344778288943E-5 3.329344778288943E-5 8 3.329344778288943E-5 0.0 0.0 3.329344778288943E-5 3.329344778288943E-5 9 3.329344778288943E-5 0.0 0.0 6.658689556577886E-5 3.329344778288943E-5 10-11 3.329344778288943E-5 0.0 0.0 6.658689556577886E-5 3.329344778288943E-5 12-13 3.329344778288943E-5 0.0 0.0 1.16527067240113E-4 2.3305413448022604E-4 14-15 3.329344778288943E-5 0.0 0.0 1.3317379113155772E-4 3.162877539374496E-4 16-17 3.329344778288943E-5 0.0 0.0 1.6646723891444715E-4 4.1616809728611787E-4 18-19 3.329344778288943E-5 0.0 0.0 2.33054134480226E-4 4.328148211775626E-4 20-21 3.329344778288943E-5 0.0 0.0 2.9964103004600487E-4 4.328148211775626E-4 22-23 3.329344778288943E-5 0.0 0.0 4.161680972861179E-4 4.6610826896045207E-4 24-25 4.9940171674334145E-5 1.6646723891444715E-5 0.0 6.492222317663438E-4 4.6610826896045207E-4 26-27 6.658689556577886E-5 3.329344778288943E-5 0.0 9.655099857037935E-4 5.160484406347861E-4 28-29 6.658689556577886E-5 3.329344778288943E-5 0.0 0.0025469487553910417 5.326951645262309E-4 30-31 6.658689556577886E-5 3.329344778288943E-5 0.0 0.0064589288698805505 5.493418884176756E-4 32-33 6.658689556577886E-5 3.329344778288943E-5 0.0 0.015398219599586361 5.659886123091203E-4 34-35 6.658689556577886E-5 3.329344778288943E-5 0.0 0.030014043176274822 5.659886123091203E-4 36-37 6.658689556577886E-5 3.329344778288943E-5 0.0 0.05629922020086603 5.659886123091203E-4 38-39 6.658689556577886E-5 3.329344778288943E-5 0.0 0.09691722649599113 5.659886123091203E-4 40-41 6.658689556577886E-5 3.329344778288943E-5 0.0 0.13954948638198106 5.659886123091203E-4 42-43 6.658689556577886E-5 6.658689556577886E-5 0.0 0.18431252692607591 5.82635336200565E-4 44-45 6.658689556577886E-5 6.658689556577886E-5 0.0 0.24259270727002386 5.992820600920097E-4 46-47 6.658689556577886E-5 6.658689556577886E-5 0.0 0.3219809335083237 5.992820600920097E-4 48-49 6.658689556577886E-5 6.658689556577886E-5 0.0 0.4047317979726954 6.658689556577886E-4 50-51 6.658689556577886E-5 6.658689556577886E-5 0.0 0.4941746454414278 6.99162403440678E-4 52-53 6.658689556577886E-5 6.658689556577886E-5 0.0 0.5813701851848152 7.324558512235675E-4 54-55 6.658689556577886E-5 6.658689556577886E-5 0.0 0.6842469388339436 7.657492990064569E-4 56-57 6.658689556577886E-5 6.658689556577886E-5 0.0 0.8103292255877459 8.822763662465699E-4 58-59 6.658689556577886E-5 6.658689556577886E-5 0.0 0.9596669856178964 9.488632618123488E-4 60-61 6.658689556577886E-5 6.658689556577886E-5 0.0 1.1138489423004574 9.655099857037936E-4 62-63 6.658689556577886E-5 6.658689556577886E-5 0.0 1.269595691028814 9.655099857037936E-4 64-65 6.658689556577886E-5 9.988034334866829E-5 0.0 1.4355468815026264 9.821567095952381E-4 66-67 6.658689556577886E-5 9.988034334866829E-5 0.0 1.632793912892355 9.98803433486683E-4 68-69 6.658689556577886E-5 9.988034334866829E-5 0.0 1.8557934261421485 9.98803433486683E-4 70-71 6.658689556577886E-5 9.988034334866829E-5 0.0 2.099301703226202 0.0010820370529439065 72-73 6.658689556577886E-5 9.988034334866829E-5 0.0 2.3451738151028403 0.001281797739641243 74-75 6.658689556577886E-5 9.988034334866829E-5 0.0 2.598420425663389 0.0013650313590984667 76-77 6.658689556577886E-5 9.988034334866829E-5 0.0 2.8757881391426405 0.0013650313590984667 78-79 6.658689556577886E-5 9.988034334866829E-5 0.0 3.2012648846681673 0.0014316182546642454 80-81 6.658689556577886E-5 9.988034334866829E-5 0.0 3.546301530766142 0.001464911702447135 82-83 6.658689556577886E-5 9.988034334866829E-5 0.0 3.9029409434164535 0.001464911702447135 84-85 6.658689556577886E-5 9.988034334866829E-5 0.0 4.268819287826517 0.001464911702447135 86-87 6.658689556577886E-5 9.988034334866829E-5 0.0 4.650878247859065 0.0015481453219043585 88-89 6.658689556577886E-5 9.988034334866829E-5 0.0 5.076701445002221 0.0016480256652530267 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11155 0.0 27.761696 1 GTATCAA 15170 0.0 26.372118 1 TATCAAC 18195 0.0 21.884941 2 TCAACGC 18215 0.0 21.860909 4 ATCAACG 18230 0.0 21.842922 3 CAACGCA 18505 0.0 21.543966 5 AACGCAG 18925 0.0 21.086355 6 GAGTACT 10925 0.0 20.674116 12-13 GTACATG 10025 0.0 19.83474 1 GTACTTT 11555 0.0 19.3416 14-15 TACATGG 9975 0.0 19.174656 2 AGAGTAC 16470 0.0 18.870749 10-11 ACGCAGA 21090 0.0 18.830168 7 CGCAGAG 21185 0.0 18.656141 8 ACATGGG 10090 0.0 18.156477 3 AGTACTT 11805 0.0 17.505062 12-13 GCAGAGT 22630 0.0 17.234257 9 TACCTGG 2260 0.0 17.010302 2 ACTTTTT 13540 0.0 16.610514 16-17 TATAGCG 235 5.175195E-5 16.156883 5 >>END_MODULE