FastQCFastQC Report
Thu 26 May 2016
SRR938677_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938677_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775865
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194110.5140808794805958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146920.3891028943036894No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT110570.29283356264061344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100130.2651842690350423No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74730.19791491486056836No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61510.16290306989259415No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50200.1329496684865587No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40480.1072072227158545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG195700.025.1714341
TACATGG198900.024.1815532
GTATCAA308850.023.7325921
ACATGGG201000.023.0801983
GAGTACT151700.022.3216312-13
GTGTAGC19900.021.9188631
GTACTTT160700.020.95404214-15
GGTATCA234100.020.3741661
ATCAACG359250.020.2867493
TCAACGC360350.020.2248234
AGTACTT158300.020.22419412-13
CAACGCA360200.020.1806225
CATGGGG85100.020.157284
TATCAAC363400.020.1463552
AACGCAG367300.019.8295146
AGAGTAC268250.019.51751110-11
CATGGGA108000.018.9545254
GTATAGG10500.018.5130441
ACTTTTT187400.018.27331416-17
ACGCAGA402450.018.0279567
Produced by FastQC (version 0.11.5)