FastQCFastQC Report
Thu 26 May 2016
SRR938677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775865
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189680.5023484684966226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166510.4409850458106951No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT138310.3663001722784051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116300.30800889332642983No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50340.13332044445444952No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47180.1249515011792No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41330.10945836252090581No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39210.1038437550071308No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39130.10363188302547892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG191350.022.3245331
TACATGG196600.021.43462
GAGTACT178700.021.43119812-13
ACATGGG198200.020.4714413
GTACTTT188450.020.24684314-15
AGTACTT183850.020.06939112-13
GGTATCA287400.020.0666921
GTATCAA358950.019.8612351
CTATACG1951.04207975E-519.4688954
AGAGTAC300250.018.97477310-11
GTGTAGC19400.018.4110221
ACTTTTT213550.018.03352516-17
TACTTTT209150.017.49419414-15
CATGGGG82600.017.3505344
CATGGGA108950.017.3357164
ATCAACG421500.016.7754693
TCAACGC422300.016.732454
TATCAAC424550.016.6773072
CAACGCA424150.016.6482815
AACGCAG430900.016.4095156
Produced by FastQC (version 0.11.5)