FastQCFastQC Report
Thu 26 May 2016
SRR938676_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938676_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3731885
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193090.5174060829848722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145630.3902317461550932No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106090.2842799282400181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100590.2695420678825848No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT73040.19571878554671432No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61610.16509083211299383No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49530.13272113154612214No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40440.108363467791746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG191750.025.2565211
GTATCAA301100.025.0022531
TACATGG194450.024.4228322
ACATGGG195350.023.1931573
GAGTACT154200.022.81154312-13
GGTATCA222900.021.5990221
ATCAACG349550.021.403873
GTACTTT162850.021.3955414-15
TCAACGC350000.021.3627954
AGTACTT158350.021.28438212-13
CAACGCA351900.021.2070065
TATCAAC353450.021.1945422
AACGCAG356350.020.9945876
AGAGTAC267150.020.3442610-11
CATGGGG80400.019.944844
CATGGGA111750.019.2318294
ACGCAGA389050.019.1339637
CGCAGAG390600.019.0337418
ACTTTTT191300.018.44897316-17
GTATAGG9150.018.179491
Produced by FastQC (version 0.11.5)