##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938676_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3731885 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.065169210733984 33.0 31.0 34.0 31.0 34.0 2 32.170131180355234 34.0 31.0 34.0 30.0 34.0 3 32.19395881705894 34.0 31.0 34.0 30.0 34.0 4 35.740208500529896 37.0 35.0 37.0 35.0 37.0 5 35.76326226558428 37.0 35.0 37.0 35.0 37.0 6 35.83591000258583 37.0 36.0 37.0 35.0 37.0 7 35.77204897792939 37.0 35.0 37.0 35.0 37.0 8 35.74815220726255 37.0 35.0 37.0 35.0 37.0 9 37.41692871028984 39.0 37.0 39.0 35.0 39.0 10-11 37.314648227370355 39.0 37.0 39.0 35.0 39.0 12-13 37.22570309642447 39.0 37.0 39.0 34.0 39.0 14-15 38.479276156687575 40.0 38.0 41.0 34.0 41.0 16-17 38.373382486330634 40.0 38.0 41.0 34.0 41.0 18-19 38.333741259443954 40.0 38.0 41.0 34.0 41.0 20-21 38.238447192236634 40.0 38.0 41.0 34.0 41.0 22-23 38.27442539092175 40.0 38.0 41.0 34.0 41.0 24-25 38.22480221121498 40.0 38.0 41.0 34.0 41.0 26-27 38.104717857061516 40.0 38.0 41.0 34.0 41.0 28-29 37.944522004295415 40.0 38.0 41.0 33.0 41.0 30-31 37.729974530297696 40.0 37.5 41.0 33.0 41.0 32-33 37.54497351874455 40.0 37.0 41.0 33.0 41.0 34-35 37.27107011604055 40.0 37.0 41.0 32.0 41.0 36-37 37.07259280497657 40.0 36.5 41.0 31.0 41.0 38-39 36.90848994542972 39.5 36.0 41.0 31.0 41.0 40-41 36.80985453731827 39.5 36.0 41.0 31.0 41.0 42-43 36.79337707351647 40.0 36.0 41.0 31.0 41.0 44-45 36.6046881133797 40.0 36.0 41.0 30.5 41.0 46-47 36.285270312456035 40.0 36.0 41.0 30.0 41.0 48-49 35.9885104980459 39.0 35.5 41.0 29.0 41.0 50-51 34.86569039506844 38.0 34.0 40.0 26.5 40.5 52-53 34.95325579432378 38.5 34.5 39.5 27.0 40.5 54-55 35.444709577063605 39.0 35.0 40.5 28.0 41.0 56-57 35.3302840253652 39.0 35.0 41.0 27.0 41.0 58-59 35.12289111266826 38.5 35.0 40.0 26.5 41.0 60-61 34.815120374823984 38.0 35.0 40.0 26.0 41.0 62-63 34.43693133630859 38.0 34.0 40.0 26.0 41.0 64-65 34.056292329479604 37.0 34.0 40.0 25.0 41.0 66-67 33.668058635247334 36.5 34.0 39.5 23.5 41.0 68-69 33.251046053134004 36.0 33.0 39.0 22.5 41.0 70-71 32.819805272670514 35.5 33.0 39.0 21.5 40.0 72-73 32.40284735462106 35.0 32.5 38.0 20.5 40.0 74-75 31.946211231053475 35.0 32.0 37.0 19.0 39.5 76-77 31.491544353590747 35.0 31.5 37.0 17.5 39.0 78-79 31.06376616642796 35.0 31.0 36.0 13.5 39.0 80-81 30.601438281190337 34.5 31.0 36.0 7.5 37.5 82-83 30.198224489768577 34.0 31.0 35.0 6.0 37.0 84-85 29.85746345345583 34.0 30.0 35.0 2.0 37.0 86-87 29.464496628379493 34.0 30.0 35.0 2.0 36.0 88-89 29.030131287539675 34.0 29.0 35.0 2.0 36.0 90-91 28.599851683532584 34.0 29.0 35.0 2.0 35.5 92-93 28.16362227131865 34.0 29.0 35.0 2.0 35.0 94-95 27.671338747040707 34.0 27.5 35.0 2.0 35.0 96-97 27.10215427324261 34.0 26.5 35.0 2.0 35.0 98-99 26.37554975568647 33.0 25.0 35.0 2.0 35.0 100-101 23.79355633949063 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07764463042458658 1101 2 0.10659769875476854 1101 3 0.17505306065331894 1101 4 0.038544939168694725 1101 5 0.06427083859110638 1101 6 0.12468759086250003 1101 7 0.13940073482676496 1101 8 0.11335146712605138 1101 9 0.20339562345348838 1101 10-11 0.1668695920122545 1101 12-13 0.14242648834289895 1101 14-15 0.14096601427216626 1101 16-17 0.30308401158929144 1101 18-19 0.39038477187958165 1101 20-21 0.4398228392913097 1101 22-23 0.3122844295125873 1101 24-25 0.30430770057336787 1101 26-27 0.2429720367734305 1101 28-29 0.3542200841777401 1101 30-31 0.4168159358820631 1101 32-33 0.27491763891057275 1101 34-35 0.2856088315170382 1101 36-37 0.3331909393478014 1101 38-39 0.329877058340287 1101 40-41 0.35952640978491957 1101 42-43 0.5004774469484374 1101 44-45 0.5082952867826265 1101 46-47 0.46326214137411625 1101 48-49 0.4435472887470979 1101 50-51 0.40987697791432964 1101 52-53 0.4280373615397153 1101 54-55 0.4159637762865813 1101 56-57 0.28231227836908346 1101 58-59 0.31322108355522715 1101 60-61 0.2855035420686036 1101 62-63 0.2623142493731976 1101 64-65 0.18689852876114088 1101 66-67 0.15123401821031734 1101 68-69 0.33823876699850786 1101 70-71 0.18946748906126487 1101 72-73 0.2554996147990778 1101 74-75 0.2296927972427376 1101 76-77 0.11304241388966574 1101 78-79 0.05617213269678345 1101 80-81 -0.05877196097435089 1101 82-83 0.015927282684550903 1101 84-85 0.10999441235980356 1101 86-87 -0.11871859219003511 1101 88-89 0.06442867589289492 1101 90-91 -0.031159878262680962 1101 92-93 -0.11246941346804462 1101 94-95 -0.049518959940211715 1101 96-97 -0.12171805324800289 1101 98-99 0.027682861904096967 1101 100-101 0.5310676594947701 1102 1 0.09920839700074424 1102 2 0.06259922028574039 1102 3 0.0886642045560464 1102 4 0.2396552632810227 1102 5 0.15258456253816632 1102 6 0.18633123781484784 1102 7 0.12170441142272637 1102 8 0.1356320584244628 1102 9 0.195252153575872 1102 10-11 0.20866775305843532 1102 12-13 0.19811550577491488 1102 14-15 0.28462280410413854 1102 16-17 0.38174781882705133 1102 18-19 0.4010420470274667 1102 20-21 0.284202028420232 1102 22-23 0.252065906184626 1102 24-25 0.24376814732634244 1102 26-27 0.23005837053961642 1102 28-29 0.19294862206517394 1102 30-31 0.3078326727227889 1102 32-33 0.288087079747946 1102 34-35 0.2551188591931606 1102 36-37 0.3064936453119458 1102 38-39 0.22979999804306317 1102 40-41 0.34636779018077846 1102 42-43 0.40203406495234617 1102 44-45 0.37390507967078435 1102 46-47 0.5033003763981867 1102 48-49 0.48829112064415625 1102 50-51 0.5277539831216345 1102 52-53 0.4130997967762724 1102 54-55 0.36593393591616774 1102 56-57 0.3125107332281445 1102 58-59 0.2170514722014616 1102 60-61 0.19082135840933034 1102 62-63 0.09613650569870202 1102 64-65 0.06627620947573831 1102 66-67 0.05389964866191832 1102 68-69 0.1923006644525671 1102 70-71 0.23146928604181483 1102 72-73 0.12351434479292323 1102 74-75 0.025145911790414033 1102 76-77 -0.035885956104692696 1102 78-79 -0.03274561281404331 1102 80-81 -0.037945022006105944 1102 82-83 -0.027301904476875194 1102 84-85 -0.05661027815292741 1102 86-87 -0.07876397578635519 1102 88-89 -0.03178487622609438 1102 90-91 -0.18452397013167499 1102 92-93 -0.3448744161569586 1102 94-95 -0.35040334981940546 1102 96-97 -0.5079085200694458 1102 98-99 -0.5857266135612171 1102 100-101 -0.20187676527470444 1103 1 0.10312837925382468 1103 2 0.23114018827603644 1103 3 0.2664322679230082 1103 4 0.23273331059745317 1103 5 0.20062457141163037 1103 6 0.14700679534228556 1103 7 0.1964170026040719 1103 8 0.20944294929393692 1103 9 0.22362439529941014 1103 10-11 0.23816483656032972 1103 12-13 0.27358071006027984 1103 14-15 0.3578229920127427 1103 16-17 0.46762205055489403 1103 18-19 0.42343579384672836 1103 20-21 0.3370027148085981 1103 22-23 0.3337121943372523 1103 24-25 0.3662411680876332 1103 26-27 0.3934582765853136 1103 28-29 0.33468945173385833 1103 30-31 0.4939812640607286 1103 32-33 0.45054287555392847 1103 34-35 0.4680450789679256 1103 36-37 0.5495928975922908 1103 38-39 0.5366120111444701 1103 40-41 0.5403677640823616 1103 42-43 0.5363851278103837 1103 44-45 0.499775461882102 1103 46-47 0.49583655532893545 1103 48-49 0.488151820342722 1103 50-51 0.5855975361601438 1103 52-53 0.4888266376933714 1103 54-55 0.4248240354816275 1103 56-57 0.44683537143883 1103 58-59 0.5138521324914151 1103 60-61 0.4491614338337513 1103 62-63 0.3966888134512274 1103 64-65 0.21950947712805657 1103 66-67 0.31083758662598626 1103 68-69 0.39730992939065146 1103 70-71 0.18875041819114813 1103 72-73 0.3205916483044575 1103 74-75 0.175659398147463 1103 76-77 0.1699261613903822 1103 78-79 0.2958830458578028 1103 80-81 0.3958044723370655 1103 82-83 0.31072544666427504 1103 84-85 0.2996438812823037 1103 86-87 0.3483340107207624 1103 88-89 0.272648918614248 1103 90-91 0.3193539284237801 1103 92-93 0.10646736871379048 1103 94-95 0.15771684644068884 1103 96-97 0.22223417302621584 1103 98-99 0.31298706070444027 1103 100-101 0.6309537385552382 1104 1 0.019668672204460336 1104 2 0.05399250765599817 1104 3 -0.0014852581775457452 1104 4 0.09966542601539885 1104 5 0.1043461902476821 1104 6 0.05975124612268701 1104 7 0.12201386537822856 1104 8 0.08407629793059357 1104 9 0.10950780512732194 1104 10-11 0.1189464836832741 1104 12-13 0.15224427989834766 1104 14-15 0.21059002732637566 1104 16-17 0.2767696464994742 1104 18-19 0.24755275447396485 1104 20-21 0.26090461299430245 1104 22-23 0.2204633291868916 1104 24-25 0.13293275227290025 1104 26-27 0.2032945221811886 1104 28-29 0.17079771460353754 1104 30-31 0.2595175574188602 1104 32-33 0.19847547124050635 1104 34-35 0.36150733951168235 1104 36-37 0.3616116532069995 1104 38-39 0.30765862630904905 1104 40-41 0.39070970741066446 1104 42-43 0.37974782853422795 1104 44-45 0.4140560446080386 1104 46-47 0.4865950225443214 1104 48-49 0.5190000170861566 1104 50-51 0.5465579808121603 1104 52-53 0.6135690721393487 1104 54-55 0.4985353477263672 1104 56-57 0.43334055053404796 1104 58-59 0.4738396693219329 1104 60-61 0.5021796509654521 1104 62-63 0.5776703078937473 1104 64-65 0.5433795558630052 1104 66-67 0.5440766082539454 1104 68-69 0.5495433108390486 1104 70-71 0.5365515023016627 1104 72-73 0.4624904558358196 1104 74-75 0.38899492334040175 1104 76-77 0.47413922866635616 1104 78-79 0.4097440219806465 1104 80-81 0.42667370564045015 1104 82-83 0.27470543150207405 1104 84-85 0.4280822011623826 1104 86-87 0.4077270331981282 1104 88-89 0.45117118627602437 1104 90-91 0.39815020058658845 1104 92-93 0.5520780044809683 1104 94-95 0.6883137290606562 1104 96-97 0.4996096174070104 1104 98-99 0.6643443818903698 1104 100-101 0.9968282045375325 1105 1 -0.017686853171319683 1105 2 0.03896461987536526 1105 3 0.07279373850912663 1105 4 0.08011144459136688 1105 5 0.11194798513119508 1105 6 0.054350832162526785 1105 7 0.061168607898224536 1105 8 0.07914322245061811 1105 9 0.08801894158268908 1105 10-11 0.11116682654305521 1105 12-13 0.06450846839017998 1105 14-15 0.22036047176468543 1105 16-17 0.2918875128122522 1105 18-19 0.18597974774557002 1105 20-21 0.21784767599314847 1105 22-23 0.12415919821323484 1105 24-25 0.18213385812182992 1105 26-27 0.15287256901664392 1105 28-29 0.2782289397792468 1105 30-31 0.22599376243400826 1105 32-33 0.20196689359259778 1105 34-35 0.1572610466992117 1105 36-37 0.3002479308491317 1105 38-39 0.23889686183690628 1105 40-41 0.3361843207648221 1105 42-43 0.2797325168000242 1105 44-45 0.35349155680747657 1105 46-47 0.46056667822655584 1105 48-49 0.45464127631164786 1105 50-51 0.605727032177235 1105 52-53 0.5603279662013918 1105 54-55 0.5504200330201456 1105 56-57 0.3470246257936793 1105 58-59 0.45489930286414904 1105 60-61 0.479040570012522 1105 62-63 0.37347222838715055 1105 64-65 0.4282716137011633 1105 66-67 0.2873995072634088 1105 68-69 0.3753221570268792 1105 70-71 0.31389316005122225 1105 72-73 0.33396805324338175 1105 74-75 0.3058173804109181 1105 76-77 0.24118340148117667 1105 78-79 0.20595593559654546 1105 80-81 0.31387420870849425 1105 82-83 0.2630864830247859 1105 84-85 0.37220405978565196 1105 86-87 0.36605515678863654 1105 88-89 0.36341851811305403 1105 90-91 0.37037180818644444 1105 92-93 0.38038875295179153 1105 94-95 0.32259989653554655 1105 96-97 0.1976462786345934 1105 98-99 0.3440315433252792 1105 100-101 0.7716739645095032 1106 1 0.0854942819130855 1106 2 0.057002666943596125 1106 3 0.08453245494803951 1106 4 0.09622749069529846 1106 5 0.06649535066513579 1106 6 0.0412503258930883 1106 7 0.02025402046800906 1106 8 -0.005067627079640147 1106 9 -0.021733920964699394 1106 10-11 0.018783197482633796 1106 12-13 0.10809144354273315 1106 14-15 0.14175157162407004 1106 16-17 0.207508755412114 1106 18-19 0.15594885515912438 1106 20-21 0.19156964090131368 1106 22-23 0.15893808745215665 1106 24-25 0.06286394900649839 1106 26-27 0.028874939160594693 1106 28-29 0.03386705219680408 1106 30-31 -0.026921418115207985 1106 32-33 0.11830806061836796 1106 34-35 0.08755165233811368 1106 36-37 0.25848768242839526 1106 38-39 0.25394557228568004 1106 40-41 0.39070738111637127 1106 42-43 0.31367212580659043 1106 44-45 0.4173594480355689 1106 46-47 0.31413080455467934 1106 48-49 0.3281431989927981 1106 50-51 0.2884040899571758 1106 52-53 0.1391502492599912 1106 54-55 0.014500639939726057 1106 56-57 0.20828255372376248 1106 58-59 0.2268055307238086 1106 60-61 0.24760392838239653 1106 62-63 0.35811730632536154 1106 64-65 0.24107889012344685 1106 66-67 0.26493276056842774 1106 68-69 0.2965065138435463 1106 70-71 0.36203565656786907 1106 72-73 0.3034033146863635 1106 74-75 0.11306018225171144 1106 76-77 0.07832549681980794 1106 78-79 0.2133672024089499 1106 80-81 0.13998322820491538 1106 82-83 0.15609276395810312 1106 84-85 0.3236686769916588 1106 86-87 0.36220289550299967 1106 88-89 0.4869480853480148 1106 90-91 0.555094595337895 1106 92-93 0.7490515021568349 1106 94-95 0.7226658547890636 1106 96-97 0.7821338328200262 1106 98-99 0.861983855028619 1106 100-101 1.252537487736145 1107 1 0.0725176926943476 1107 2 0.08752466319945285 1107 3 0.2336276885664006 1107 4 0.22722018022596302 1107 5 0.1975489123297791 1107 6 0.10752043499586961 1107 7 0.14824613475366988 1107 8 0.1277183515087401 1107 9 0.1691040277259077 1107 10-11 0.21436406096755434 1107 12-13 0.17498086480108554 1107 14-15 0.3235054401283577 1107 16-17 0.3224145636360305 1107 18-19 0.34645220756996054 1107 20-21 0.311834736037909 1107 22-23 0.2862691276901046 1107 24-25 0.2331210024731476 1107 26-27 0.2816679578017087 1107 28-29 0.2702850992944619 1107 30-31 0.23375093364794708 1107 32-33 0.32612141663275196 1107 34-35 0.33215903943550273 1107 36-37 0.3467515082707564 1107 38-39 0.25710430816857155 1107 40-41 0.21426402163474734 1107 42-43 0.18118235596674026 1107 44-45 0.39646617385846383 1107 46-47 0.44518743989458187 1107 48-49 0.38478074392274664 1107 50-51 0.5107871678513831 1107 52-53 0.31906666132945816 1107 54-55 0.22762714432836617 1107 56-57 0.37688463351944534 1107 58-59 0.4287310279091585 1107 60-61 0.3543880508718331 1107 62-63 0.3858145616068569 1107 64-65 0.4183858187153575 1107 66-67 0.33678092207963317 1107 68-69 0.3759070629756778 1107 70-71 0.5902425472533466 1107 72-73 0.5790922071656652 1107 74-75 0.6606519342980626 1107 76-77 0.5525741786641483 1107 78-79 0.5699342465830775 1107 80-81 0.43914410817675886 1107 82-83 0.45006364302321344 1107 84-85 0.6322530109900413 1107 86-87 0.5565996590031297 1107 88-89 0.6898468350771445 1107 90-91 0.7888161939938136 1107 92-93 0.854715303423152 1107 94-95 0.8654418125279015 1107 96-97 0.9526918725956541 1107 98-99 1.167821058352974 1107 100-101 1.6179168591120892 1108 1 0.20538708684293283 1108 2 0.2252529327019488 1108 3 0.12056522758084043 1108 4 0.16491314576004612 1108 5 0.24875520086197866 1108 6 0.15851232043221586 1108 7 0.21510223722926014 1108 8 0.17048627349356593 1108 9 0.21453235066796594 1108 10-11 0.26352099003815255 1108 12-13 0.3109913082689033 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-1.2689116963620393 2316 74-75 -1.1937818214119247 2316 76-77 -1.1902729982967166 2316 78-79 -1.0247595585593636 2316 80-81 -1.077661058248669 2316 82-83 -1.033329823258601 2316 84-85 -1.0668935596331934 2316 86-87 -1.1362615195885475 2316 88-89 -1.116169598675743 2316 90-91 -1.5965470583910495 2316 92-93 -1.4787276446925155 2316 94-95 -1.5244126833710716 2316 96-97 -1.5330906880733757 2316 98-99 -1.7317257402464428 2316 100-101 -1.896466307330268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14715.0 3 3117.0 4 2683.0 5 2902.0 6 3337.0 7 3814.0 8 4787.0 9 5660.0 10 6704.0 11 8732.0 12 11563.0 13 15686.0 14 17611.0 15 20635.0 16 26888.0 17 34506.0 18 30400.0 19 19895.0 20 19007.0 21 19697.0 22 20945.0 23 23253.0 24 26689.0 25 30411.0 26 35562.0 27 42133.0 28 50621.0 29 61943.0 30 76602.0 31 96729.0 32 125553.0 33 168479.0 34 236693.0 35 351506.0 36 527197.0 37 779542.0 38 695478.0 39 110151.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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22.38912670019562 18-19 21.604866834146566 27.16091764521399 29.780373274675988 21.453842245963457 20-21 22.775204475421006 25.973971938827894 29.632216030518677 21.618607555232423 22-23 22.151518947756962 26.137658295196665 29.6826731235491 22.028149633497264 24-25 22.196932165273722 26.249897626959353 29.803591447655492 21.749578760111437 26-27 21.885022583908817 26.386321924723173 29.92543977068812 21.803215720679894 28-29 21.516967548217096 26.883942539873456 29.839404438869476 21.75968547303997 30-31 21.738150427537796 26.412938001914977 29.733233501081607 22.115678069465623 32-33 21.551458963546715 26.540024533026983 29.72890199941486 22.179614504011443 34-35 22.291885849685443 26.48099168243709 29.50204440881509 21.725078059062373 36-37 22.155045205651493 26.22913412063249 29.630254640978194 21.985566032737825 38-39 21.83012463682268 26.698418199850476 29.63949067586642 21.831966487460424 40-41 21.98849354826701 26.598533047685695 29.598868089197644 21.814105314849648 42-43 21.700393156429744 26.809329550320193 29.046079761887956 22.444197531362107 44-45 21.883494166641455 26.702470144161765 29.060605755042634 22.353429934154136 46-47 22.21552642480317 26.96124679512648 28.75074571418545 22.0724810658849 48-49 22.22922997457441 27.01937237105627 28.482039705984597 22.269357948384723 50-51 21.84927160578326 27.23124137974621 28.713753585091023 22.205733429379507 52-53 22.26396867532383 27.433228721422275 28.153965747947375 22.14883685530652 54-55 21.87761778995936 27.073719923400187 28.477964645868397 22.570697640772057 56-57 22.157112976364264 27.30096446795362 28.180013225590965 22.36190933009115 58-59 22.51065128043847 26.96923023162671 28.140051233666917 22.380067254267903 60-61 22.198288139838102 27.054936243259135 28.768220626377865 21.9785549905249 62-63 22.033095973213463 27.118343278796296 28.672094153519765 22.17646659447047 64-65 21.811900963246593 27.31339291081816 28.426419286826597 22.448286839108647 66-67 21.866876479449108 27.266354637400475 28.0795002151926 22.78726866795782 68-69 21.88613411142713 27.063780897768662 28.416922195014283 22.63316279578992 70-71 22.097231033758785 26.962414188112383 28.255485229144856 22.684869548983976 72-73 22.045608106290704 26.900194308252935 28.476339796288347 22.577857789168014 74-75 21.766494654867373 27.54870813633921 28.09744962428341 22.58734758451001 76-77 21.92186385634576 27.08367173288899 28.16647859719176 22.82798581357349 78-79 21.938266521426605 27.12745299291451 28.209534464200086 22.724746021458802 80-81 22.06255159303148 27.209364857851952 28.00207582125351 22.72600772786306 82-83 21.985748660541894 27.155758459749567 28.25752393061171 22.600968949096828 84-85 21.91287803664649 27.22199999327711 28.046629982083495 22.818491987992914 86-87 21.885919675957766 27.269534627603797 28.095465320631835 22.7490803758066 88-89 21.913542066133083 27.125694701791204 27.80727292872237 23.153490303353337 90-91 21.932556096558542 26.970917637540193 28.006695382682057 23.089830883219207 92-93 22.252191939000927 27.02912211891516 28.01137600001291 22.707309942071 94-95 21.985745110700474 26.783982866049687 28.095215871069286 23.135056152180553 96-97 21.872030953936004 26.692564728566648 28.329568282545047 23.105836034952297 98-99 21.920174973406855 26.87149630533839 27.770155612764007 23.438173108490748 100-101 22.073912141998314 26.148540697475852 28.322589372178147 23.454957788347684 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4834.0 1 3178.5 2 1713.0 3 1936.5 4 1736.5 5 1664.0 6 2681.0 7 5170.5 8 7844.0 9 8571.0 10 6990.5 11 5376.5 12 5012.0 13 5193.0 14 5507.0 15 5726.5 16 5922.0 17 6062.0 18 6385.5 19 6898.5 20 7577.5 21 8641.5 22 10166.0 23 11966.5 24 14021.5 25 17061.5 26 21663.0 27 27273.0 28 32122.5 29 37470.0 30 43968.0 31 51083.0 32 60180.5 33 68268.5 34 75879.5 35 82196.0 36 89036.5 37 96663.5 38 100499.0 39 102836.0 40 105635.0 41 110757.5 42 114714.0 43 118383.0 44 123096.5 45 128147.5 46 130185.0 47 138116.0 48 160886.0 49 160584.5 50 139369.5 51 128586.5 52 122422.5 53 120129.0 54 117481.5 55 109329.0 56 100463.0 57 91768.0 58 84361.5 59 75469.5 60 63601.5 61 52273.0 62 42883.5 63 33395.5 64 24452.0 65 18945.5 66 14620.5 67 11307.5 68 8541.0 69 6287.5 70 4689.0 71 3568.0 72 2883.5 73 2466.0 74 1953.5 75 1543.5 76 1196.0 77 755.0 78 504.0 79 365.5 80 270.5 81 222.0 82 169.0 83 124.5 84 104.5 85 77.5 86 57.5 87 46.0 88 34.0 89 22.5 90 15.5 91 16.0 92 14.0 93 9.5 94 8.0 95 6.5 96 4.5 97 2.0 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24247799704438908 2 0.14488656536843983 3 0.17128073346311584 4 0.14255530382099127 5 0.20726790884499388 6 0.17168267510922763 7 0.21402052849967243 8 0.22015683763031285 9 0.13205122880260242 10-11 0.1814096629451336 12-13 0.18753257402090365 14-15 0.15228229165689725 16-17 0.2580331387489164 18-19 0.20517781228521245 20-21 0.17576908184469778 22-23 0.21722266361369658 24-25 0.2079378115885136 26-27 0.30914671807946925 28-29 0.281010802851642 30-31 0.2583412940109355 32-33 0.1686815108182594 34-35 0.3243803064671071 36-37 0.35997893825774374 38-39 0.34297680662721386 40-41 0.3068288545868911 42-43 0.33836787575179833 44-45 0.34761253361237016 46-47 0.3519535033903778 48-49 0.3692637902829267 50-51 0.36267194728669294 52-53 0.36277913172565607 54-55 0.3574065117226281 56-57 0.3371084585939813 58-59 0.31284458122369796 60-61 0.282779346094534 62-63 0.3443970004434756 64-65 0.3776509726317933 66-67 0.3827824276471542 68-69 0.38650708690112373 70-71 0.38528786390791786 72-73 0.3773160212600335 74-75 0.373082235920989 76-77 0.35909466663629774 78-79 0.335634672558238 80-81 0.34499991291264337 82-83 0.3541641824439928 84-85 0.3549814637910868 86-87 0.35131039675659886 88-89 0.35861233666096354 90-91 0.37779835123536765 92-93 0.37669971073599534 94-95 0.38461796116439817 96-97 0.38481893198745404 98-99 0.3829566023604693 100-101 0.35804961835640703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3731885.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.900354385697575 #Duplication Level Percentage of deduplicated Percentage of total 1 68.82910272565616 30.21622001705888 2 15.52624079261419 13.63214946146874 3 6.065705761227616 7.988598975517795 4 3.0653277784076396 5.382759031216738 5 1.7546362558555257 3.851457672502555 6 1.0722941038060294 2.8244454697667205 7 0.7436335391003562 2.2852043129717297 8 0.4861604337095148 1.7074092262521707 9 0.3794652197846649 1.4992791863034067 >10 1.837777772471123 14.601310090474485 >50 0.1489487203829395 4.577241219367854 >100 0.0838611970372461 6.680125542335809 >500 0.004400807120558967 1.2478647373429508 >1k 0.0020781589023782486 1.6811626471593586 >5k 1.2224464131636756E-4 0.3613052055607994 >10k+ 2.444892826327351E-4 1.463467204700037 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19309 0.5174060829848722 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14563 0.3902317461550932 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10609 0.2842799282400181 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10059 0.2695420678825848 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7304 0.19571878554671432 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6161 0.16509083211299383 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4953 0.13272113154612214 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4044 0.108363467791746 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.6796109740787833E-5 0.0 5 0.0 0.0 0.0 2.6796109740787833E-5 0.0 6 0.0 0.0 0.0 2.6796109740787833E-5 0.0 7 0.0 0.0 0.0 2.6796109740787833E-5 0.0 8 0.0 0.0 0.0 2.6796109740787833E-5 0.0 9 0.0 0.0 0.0 2.6796109740787833E-5 0.0 10-11 0.0 0.0 0.0 2.6796109740787833E-5 0.0 12-13 0.0 0.0 0.0 2.6796109740787833E-5 0.0 14-15 0.0 0.0 0.0 4.019416461118175E-5 0.0 16-17 0.0 0.0 0.0 8.03883292223635E-5 0.0 18-19 0.0 0.0 0.0 1.0718443896315133E-4 0.0 20-21 0.0 0.0 0.0 2.6796109740787836E-4 0.0 22-23 0.0 0.0 0.0 4.8232997533418097E-4 0.0 24-25 0.0 0.0 0.0 8.172813470940289E-4 0.0 26-27 0.0 0.0 0.0 0.001286213267557816 0.0 28-29 0.0 0.0 0.0 0.002746601248430753 0.0 30-31 1.3398054870393917E-5 0.0 0.0 0.0068196099290305035 0.0 32-33 2.6796109740787833E-5 0.0 0.0 0.01622504444804703 0.0 34-35 2.6796109740787833E-5 0.0 0.0 0.030949506750609944 0.0 36-37 2.6796109740787833E-5 0.0 0.0 0.05746425733911951 0.0 38-39 2.6796109740787833E-5 0.0 0.0 0.09764502389543087 0.0 40-41 2.6796109740787833E-5 0.0 0.0 0.14274287658917678 0.0 42-43 2.6796109740787833E-5 0.0 0.0 0.19115004883591 0.0 44-45 2.6796109740787833E-5 0.0 0.0 0.25150828602703457 0.0 46-47 2.6796109740787833E-5 0.0 0.0 0.32931079065941204 0.0 48-49 2.6796109740787833E-5 0.0 0.0 0.4110255273139446 0.0 50-51 2.6796109740787833E-5 0.0 0.0 0.5047985133518316 0.0 52-53 2.6796109740787833E-5 0.0 0.0 0.5983705285666627 0.0 54-55 2.6796109740787833E-5 0.0 0.0 0.7025538032388458 0.0 56-57 2.6796109740787833E-5 0.0 0.0 0.833814546804095 0.0 58-59 2.6796109740787833E-5 0.0 0.0 0.9811797523235577 0.0 60-61 2.6796109740787833E-5 0.0 0.0 1.1436847598465656 0.0 62-63 2.6796109740787833E-5 0.0 0.0 1.3081324853257803 0.0 64-65 2.6796109740787833E-5 0.0 0.0 1.4820526355983639 0.0 66-67 2.6796109740787833E-5 0.0 0.0 1.686506952920575 0.0 68-69 2.6796109740787833E-5 0.0 0.0 1.923572135797325 0.0 70-71 2.6796109740787833E-5 0.0 0.0 2.1846332349469506 0.0 72-73 2.6796109740787833E-5 0.0 0.0 2.4471413240225783 0.0 74-75 2.6796109740787833E-5 0.0 0.0 2.716870964673349 0.0 76-77 2.6796109740787833E-5 0.0 0.0 3.017067782099395 0.0 78-79 2.6796109740787833E-5 0.0 0.0 3.3670785675335653 0.0 80-81 2.6796109740787833E-5 0.0 0.0 3.7460023553780464 0.0 82-83 2.6796109740787833E-5 0.0 0.0 4.129173326616442 0.0 84-85 2.6796109740787833E-5 0.0 0.0 4.517234587882531 0.0 86-87 2.6796109740787833E-5 0.0 0.0 4.920301670603461 0.0 88-89 2.6796109740787833E-5 0.0 0.0 5.36511709230054 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 19175 0.0 25.256521 1 GTATCAA 30110 0.0 25.002253 1 TACATGG 19445 0.0 24.422832 2 ACATGGG 19535 0.0 23.193157 3 GAGTACT 15420 0.0 22.811543 12-13 GGTATCA 22290 0.0 21.599022 1 ATCAACG 34955 0.0 21.40387 3 GTACTTT 16285 0.0 21.39554 14-15 TCAACGC 35000 0.0 21.362795 4 AGTACTT 15835 0.0 21.284382 12-13 CAACGCA 35190 0.0 21.207006 5 TATCAAC 35345 0.0 21.194542 2 AACGCAG 35635 0.0 20.994587 6 AGAGTAC 26715 0.0 20.34426 10-11 CATGGGG 8040 0.0 19.94484 4 CATGGGA 11175 0.0 19.231829 4 ACGCAGA 38905 0.0 19.133963 7 CGCAGAG 39060 0.0 19.033741 8 ACTTTTT 19130 0.0 18.448973 16-17 GTATAGG 915 0.0 18.17949 1 >>END_MODULE