FastQCFastQC Report
Thu 26 May 2016
SRR938676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3731885
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190750.5111357933055279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165030.44221619905222165No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT138940.3723051487385061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115760.31019176635935997No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49430.13245317044871424No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48520.13001472446230256No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40670.1089797783157841No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39000.10450482798907254No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37950.10169123646628982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT177800.021.7103512-13
GTACATG189200.021.5971641
GTATCAA349100.020.9298481
TACATGG195300.020.7550932
AGTACTT186400.020.50504112-13
GGTATCA274150.020.318581
GTACTTT190150.020.23790614-15
ACATGGG195300.020.0259913
AGAGTAC299750.019.25723610-11
GTGTAGG14650.019.157511
GTACCGT2302.081013E-618.5675986
ACTTTTT215900.017.92270916-17
TACTTTT212100.017.79674114-15
TATCAAC411950.017.651572
ATCAACG413300.017.5479743
TCAACGC414250.017.5421074
CAACGCA415150.017.5269455
AACGCAG422250.017.2833426
GTGTAGC19400.017.1640991
TGCACCG7250.017.0217575
Produced by FastQC (version 0.11.5)