##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938676_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3731885 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.741831540896893 31.0 31.0 34.0 30.0 34.0 2 31.968101910964567 31.0 31.0 34.0 30.0 34.0 3 32.02750191927136 33.0 31.0 34.0 30.0 34.0 4 35.730070192409464 37.0 35.0 37.0 33.0 37.0 5 35.62789742985113 37.0 35.0 37.0 33.0 37.0 6 35.67124120920125 37.0 35.0 37.0 33.0 37.0 7 35.585821642413954 37.0 35.0 37.0 33.0 37.0 8 35.56670797733585 37.0 35.0 37.0 33.0 37.0 9 37.15426466785552 39.0 37.0 39.0 33.0 39.0 10-11 37.05364581170106 39.0 37.0 39.0 33.0 39.0 12-13 36.95666265171622 39.0 37.0 39.0 33.0 39.0 14-15 38.03151718233546 40.0 37.0 41.0 33.0 41.0 16-17 37.93042162338872 40.0 37.0 41.0 33.0 41.0 18-19 38.124410988012755 40.0 37.0 41.0 33.0 41.0 20-21 38.13432635250014 40.0 37.0 41.0 33.0 41.0 22-23 38.04014887918572 40.0 37.0 41.0 33.0 41.0 24-25 37.94340340069429 40.0 37.0 41.0 33.0 41.0 26-27 37.72083812872047 40.0 37.0 41.0 32.0 41.0 28-29 37.511086354483055 39.5 36.5 41.0 32.0 41.0 30-31 37.236824553811275 39.0 36.0 41.0 31.0 41.0 32-33 37.02756167459608 39.0 36.0 41.0 31.0 41.0 34-35 36.963597618897694 39.0 35.5 41.0 31.0 41.0 36-37 36.81403406053509 39.0 35.0 41.0 30.0 41.0 38-39 36.651183517176975 39.0 35.0 41.0 30.0 41.0 40-41 36.432023896770666 39.0 35.0 40.5 30.0 41.0 42-43 36.17503433251561 39.0 35.0 40.0 30.0 41.0 44-45 35.849441770043825 38.5 35.0 40.0 28.5 41.0 46-47 35.456233779979826 38.0 35.0 40.0 27.0 41.0 48-49 35.069033611700256 38.0 34.0 40.0 26.0 41.0 50-51 34.784246701063935 38.0 34.0 40.0 25.0 41.0 52-53 34.52208883714262 38.0 34.0 40.0 24.0 41.0 54-55 34.19485099353276 38.0 33.0 40.0 23.5 41.0 56-57 33.86513852918833 37.5 33.0 40.0 23.0 41.0 58-59 33.501624916094684 37.0 32.5 40.0 22.5 41.0 60-61 33.061909062042375 36.5 32.0 39.0 21.5 40.5 62-63 32.65321318850929 36.0 31.0 39.0 20.0 40.0 64-65 32.20090209103442 35.5 31.0 39.0 18.5 40.0 66-67 31.73350063037848 35.0 31.0 38.0 17.5 40.0 68-69 31.30739331463858 35.0 30.0 38.0 13.5 40.0 70-71 30.797225262836342 34.5 29.5 37.0 9.5 39.0 72-73 30.23933320560521 34.0 29.0 36.5 7.5 39.0 74-75 29.685555958985873 34.0 28.5 36.0 7.0 39.0 76-77 27.905215728780497 31.5 26.0 34.5 2.0 36.5 78-79 28.874454598681364 33.5 28.0 35.0 2.0 37.0 80-81 28.892291027188673 34.0 28.5 35.0 2.0 37.0 82-83 28.47515478102889 33.5 27.5 35.0 2.0 36.5 84-85 27.972344405039276 33.0 26.5 35.0 2.0 36.0 86-87 27.25094248616986 33.0 26.0 35.0 2.0 35.5 88-89 26.501779797608982 32.0 24.5 34.5 2.0 35.0 90-91 25.82237676134179 32.0 23.5 34.0 2.0 35.0 92-93 25.351991151924565 31.0 20.5 34.0 2.0 35.0 94-95 24.788940307646136 31.0 19.0 34.0 2.0 35.0 96-97 23.87284361656375 31.0 6.5 34.0 2.0 35.0 98-99 22.684161221473865 31.0 2.0 34.0 2.0 35.0 100-101 18.872329935140016 24.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07630135172125208 1101 2 0.1353142681733175 1101 3 0.02156987103504804 1101 4 0.09075797116285855 1101 5 -0.07795753024907981 1101 6 0.11368883217765102 1101 7 0.1864869504876694 1101 8 0.2187731092422709 1101 9 0.2547958762027491 1101 10-11 0.30086219219047194 1101 12-13 0.2189899218497331 1101 14-15 0.29985671406881664 1101 16-17 0.3814682910835572 1101 18-19 0.3991772887852889 1101 20-21 0.3015663722940545 1101 22-23 0.2900412320437553 1101 24-25 0.286194508816898 1101 26-27 0.1292115254085786 1101 28-29 0.3185549110535746 1101 30-31 0.3285194511363798 1101 32-33 0.4859371795447629 1101 34-35 0.4097101794110003 1101 36-37 0.44369240826939915 1101 38-39 0.5500886001866192 1101 40-41 0.5027047011155119 1101 42-43 0.7041448793494354 1101 44-45 0.5915943684368656 1101 46-47 0.5532739009681436 1101 48-49 0.4723863295186348 1101 50-51 0.48486045066075434 1101 52-53 0.3865172485249033 1101 54-55 0.4123255296231818 1101 56-57 0.46407060734699 1101 58-59 0.48296495093880054 1101 60-61 0.5088694058430505 1101 62-63 0.43774460146973126 1101 64-65 0.3762120202848891 1101 66-67 0.38075918767023254 1101 68-69 0.4877418641590445 1101 70-71 0.6795442747083982 1101 72-73 0.5096181503114963 1101 74-75 0.4187213528602669 1101 76-77 0.36855057785782463 1101 78-79 0.48881707012699493 1101 80-81 0.642639981435245 1101 82-83 0.8422278229460503 1101 84-85 0.7801206307829744 1101 86-87 0.8025798927882164 1101 88-89 0.8600479035570636 1101 90-91 0.6810466433807782 1101 92-93 0.768710513275515 1101 94-95 0.9004806796152529 1101 96-97 0.8416329405947209 1101 98-99 0.6753084719833886 1101 100-101 1.1855214225867705 1102 1 0.20586955172387533 1102 2 0.16717460212313284 1102 3 0.1866507351597093 1102 4 0.0926061066315853 1102 5 -1.4958418473298707E-4 1102 6 0.009363618048396916 1102 7 0.06785541019988983 1102 8 0.13827362734980397 1102 9 0.18631058652161414 1102 10-11 0.20676763428703993 1102 12-13 0.20846184566824633 1102 14-15 0.2995756899210775 1102 16-17 0.3559459206432294 1102 18-19 0.3285795632117612 1102 20-21 0.32017856552848656 1102 22-23 0.21783179855171397 1102 24-25 0.33966550135352236 1102 26-27 0.2603953683236213 1102 28-29 0.31102497230831005 1102 30-31 0.33299058795612524 1102 32-33 0.4032524643727413 1102 34-35 0.5279128053635986 1102 36-37 0.3845887998799853 1102 38-39 0.5307310535241498 1102 40-41 0.5779021066428669 1102 42-43 0.47938753730664985 1102 44-45 0.39357301453663496 1102 46-47 0.47609129101785896 1102 48-49 0.4220738254196803 1102 50-51 0.44465760172516866 1102 52-53 0.2780527487282143 1102 54-55 0.2881364662828929 1102 56-57 0.26855223748380297 1102 58-59 0.30315671630823005 1102 60-61 0.3607903386989406 1102 62-63 0.27340089286914804 1102 64-65 0.36478807715866424 1102 66-67 0.4731938339798667 1102 68-69 0.527030154928859 1102 70-71 0.5660957772202337 1102 72-73 0.5271574018756553 1102 74-75 0.6498369892143643 1102 76-77 0.7177832540471769 1102 78-79 0.718729254179781 1102 80-81 0.8814901762174401 1102 82-83 0.7965946824274184 1102 84-85 0.8709970195079073 1102 86-87 0.9680945899904358 1102 88-89 0.9717223746282997 1102 90-91 1.0975795202548184 1102 92-93 1.3448349377826574 1102 94-95 1.2566625550333441 1102 96-97 1.2968710733089495 1102 98-99 1.4357552249575853 1102 100-101 1.8678844520074094 1103 1 0.24791344926148895 1103 2 0.19337741292265775 1103 3 0.14367186182837344 1103 4 0.036390116131407524 1103 5 0.05340958745150459 1103 6 0.06748675038041796 1103 7 0.06603830790714937 1103 8 0.08618640643975084 1103 9 0.1904674119101557 1103 10-11 0.25877753863633046 1103 12-13 0.2266850654299688 1103 14-15 0.3485451286746155 1103 16-17 0.225220906615327 1103 18-19 0.0512408188066118 1103 20-21 0.20111334558408345 1103 22-23 0.28458730858002923 1103 24-25 0.26995695542682796 1103 26-27 0.3846000190615584 1103 28-29 0.32170085606595933 1103 30-31 0.34756948999571335 1103 32-33 0.42418339491180745 1103 34-35 0.3076512992239486 1103 36-37 0.3739628293451034 1103 38-39 0.4271529605354871 1103 40-41 0.5620163263654376 1103 42-43 0.46556287952214603 1103 44-45 0.6230152913625417 1103 46-47 0.6026239205638788 1103 48-49 0.5535481902494581 1103 50-51 0.5195538605170782 1103 52-53 0.5400537796156897 1103 54-55 0.46920728433777725 1103 56-57 0.39101938610123455 1103 58-59 0.5023594096648836 1103 60-61 0.7181840202721546 1103 62-63 0.6358889854663374 1103 64-65 0.7457668982931622 1103 66-67 0.6673689935064395 1103 68-69 0.643712915619421 1103 70-71 0.706096821156919 1103 72-73 0.8404007174185679 1103 74-75 0.9249531271705962 1103 76-77 0.927748282168217 1103 78-79 0.6656884623538026 1103 80-81 1.019802652565751 1103 82-83 0.9857243654621435 1103 84-85 1.1085490532270619 1103 86-87 1.1723652697237128 1103 88-89 1.0472309675321405 1103 90-91 1.1339581038937183 1103 92-93 1.0918049115537478 1103 94-95 1.150451784216596 1103 96-97 1.2648711516042042 1103 98-99 1.601578042803684 1103 100-101 2.0140385240129426 1104 1 0.14394336086824566 1104 2 0.17707765120469787 1104 3 0.09032473345111214 1104 4 0.09765638473520255 1104 5 -0.09128370876575076 1104 6 0.04457383512335866 1104 7 -0.018720505948408572 1104 8 0.04826106108955486 1104 9 0.10255959702659112 1104 10-11 0.08231678769730166 1104 12-13 -0.08153831108344178 1104 14-15 0.052486242183391596 1104 16-17 0.20647038566278297 1104 18-19 0.193598726105499 1104 20-21 0.2041629190975911 1104 22-23 0.15458765257409368 1104 24-25 0.1762533698179567 1104 26-27 0.08298635614662686 1104 28-29 0.11927291951433716 1104 30-31 0.03255540608497398 1104 32-33 0.11430110703375362 1104 34-35 0.16125232168014492 1104 36-37 0.22284875165272666 1104 38-39 0.16244688361577175 1104 40-41 0.2728997031169982 1104 42-43 0.29084915534981093 1104 44-45 0.2764842068683393 1104 46-47 0.29619136390738987 1104 48-49 0.32104194212653425 1104 50-51 0.15999320708712617 1104 52-53 0.2345372394550438 1104 54-55 0.2514186898578288 1104 56-57 0.007731080708595073 1104 58-59 0.3264686965784236 1104 60-61 0.4890513616081975 1104 62-63 0.4346888431057181 1104 64-65 0.23978761996624343 1104 66-67 0.2948944722204878 1104 68-69 0.328164384011 1104 70-71 0.34931563974900115 1104 72-73 0.4223292539752137 1104 74-75 0.4239177711962796 1104 76-77 0.4899924861990357 1104 78-79 0.472997145425456 1104 80-81 0.5764614514707915 1104 82-83 0.5406481112399462 1104 84-85 0.5921816834453502 1104 86-87 0.7583884602157127 1104 88-89 0.8106947628186276 1104 90-91 0.8497189195301047 1104 92-93 1.0986049464823786 1104 94-95 1.0797936177314078 1104 96-97 1.2050959649543458 1104 98-99 1.4747811699743174 1104 100-101 1.917571144572939 1105 1 0.032010263261863514 1105 2 0.06573659859268588 1105 3 0.05155691498504922 1105 4 0.054050411757955885 1105 5 -0.047556826113073214 1105 6 -0.04046140935967202 1105 7 0.04672278872307345 1105 8 0.12259530832634624 1105 9 0.0619378083829929 1105 10-11 0.1604428657867487 1105 12-13 0.13850760971738652 1105 14-15 0.15164091531288904 1105 16-17 0.14813781715243124 1105 18-19 0.22798872402771764 1105 20-21 0.14900342764880037 1105 22-23 0.12723709189229027 1105 24-25 0.1537281002194959 1105 26-27 0.22079936131438416 1105 28-29 0.2541629152762823 1105 30-31 0.11324196094215466 1105 32-33 0.19359991732706305 1105 34-35 0.2869926561397378 1105 36-37 0.31878612764175074 1105 38-39 0.43043205054509315 1105 40-41 0.3344848531831204 1105 42-43 0.4185607692118367 1105 44-45 0.37847901357847746 1105 46-47 0.26630196189247357 1105 48-49 0.4496490862321494 1105 50-51 0.4981127938915648 1105 52-53 0.443591573919619 1105 54-55 0.5113330541236536 1105 56-57 0.4086543736989938 1105 58-59 0.4975555272693981 1105 60-61 0.6974062267268053 1105 62-63 0.6041803796248217 1105 64-65 0.5877698554741997 1105 66-67 0.5663689666943235 1105 68-69 0.6726550007786507 1105 70-71 0.6404658436065134 1105 72-73 0.7249500677477343 1105 74-75 0.7517439644870549 1105 76-77 0.8576249006232004 1105 78-79 0.6427767834505609 1105 80-81 0.6652669241032427 1105 82-83 0.8960982703340363 1105 84-85 0.8839082212403291 1105 86-87 0.9692852106847241 1105 88-89 0.8051085656105599 1105 90-91 0.7420853163026351 1105 92-93 0.9071876153798364 1105 94-95 1.2943835467082678 1105 96-97 1.2357908532179636 1105 98-99 1.4053200016189535 1105 100-101 1.9713815472041212 1106 1 0.09697298894555573 1106 2 0.02885056923355478 1106 3 -0.01864308518337765 1106 4 0.05994584363390487 1106 5 -0.0894926587472753 1106 6 -0.004930821462522772 1106 7 -0.03946259776009953 1106 8 0.018130766329065295 1106 9 -0.005575277191660177 1106 10-11 0.014881415101619666 1106 12-13 0.005426695290609018 1106 14-15 0.0698297490897346 1106 16-17 0.1497490071754939 1106 18-19 -0.1673741943872571 1106 20-21 -0.06677395890537241 1106 22-23 0.07877565463591196 1106 24-25 0.07030639176598896 1106 26-27 0.05343855237158124 1106 28-29 0.16684304942378958 1106 30-31 0.09896082007296059 1106 32-33 0.07897681964323056 1106 34-35 0.02139829369829016 1106 36-37 -0.018736157237142947 1106 38-39 0.05046395822271421 1106 40-41 0.16885782903334245 1106 42-43 0.04228739498853429 1106 44-45 0.12100563964020239 1106 46-47 0.07769941465495123 1106 48-49 -0.029580574226649503 1106 50-51 0.0927554056829365 1106 52-53 0.10398802823918629 1106 54-55 0.24760553309744182 1106 56-57 0.09597604041521635 1106 58-59 0.22940413267210857 1106 60-61 0.2943014081013189 1106 62-63 0.501050614502148 1106 64-65 0.37275394924815686 1106 66-67 0.334680657597179 1106 68-69 0.35546482367406895 1106 70-71 0.411738011963692 1106 72-73 0.43539378826223896 1106 74-75 0.6264024238792345 1106 76-77 0.4461404298662792 1106 78-79 0.36816764051723894 1106 80-81 0.34002066732482206 1106 82-83 0.6492368421985759 1106 84-85 0.5966655010214161 1106 86-87 0.9137654240831878 1106 88-89 0.8482335513186321 1106 90-91 0.7472747836855262 1106 92-93 0.9128373394816869 1106 94-95 1.2650986198145873 1106 96-97 1.3985352645969087 1106 98-99 1.7212054918202178 1106 100-101 2.0470008839978355 1107 1 0.060154769374623385 1107 2 0.030170311843988884 1107 3 0.03705625378266575 1107 4 -3.8652623587154267E-4 1107 5 -0.028522568079871746 1107 6 0.0022724033306502633 1107 7 0.05000266760465166 1107 8 0.08324403761024257 1107 9 0.01241888643357214 1107 10-11 -0.029033519732792 1107 12-13 0.060506421415453815 1107 14-15 0.045061672922457774 1107 16-17 0.07960312720664575 1107 18-19 0.0962108655998577 1107 20-21 0.022990856159275097 1107 22-23 0.08151630386428366 1107 24-25 0.06624148964282739 1107 26-27 0.08480437271513352 1107 28-29 0.14929453530188397 1107 30-31 0.1335880103255107 1107 32-33 0.1402682096039527 1107 34-35 0.27579563718758493 1107 36-37 0.21028432192611746 1107 38-39 0.16421820732819725 1107 40-41 0.038887971764800966 1107 42-43 0.08012827977402281 1107 44-45 0.17343094649155688 1107 46-47 0.13450818334263914 1107 48-49 0.2534941928572536 1107 50-51 0.1451466682398177 1107 52-53 0.11423085058937943 1107 54-55 0.24546077595553584 1107 56-57 0.18374373497902496 1107 58-59 0.3571945203140032 1107 60-61 0.3943942397760978 1107 62-63 0.3175843079633154 1107 64-65 0.23969220853364703 1107 66-67 0.2503889890679325 1107 68-69 0.36586550597652234 1107 70-71 0.42544987776008014 1107 72-73 0.19946455575961863 1107 74-75 0.35814992118926625 1107 76-77 0.24886460152249157 1107 78-79 0.2550299639872158 1107 80-81 0.3873184824695812 1107 82-83 0.38921166612542635 1107 84-85 0.398847904003361 1107 86-87 0.4850852475446459 1107 88-89 0.45678911304381487 1107 90-91 0.7810176575197758 1107 92-93 0.8137984777357978 1107 94-95 0.947346748664657 1107 96-97 1.0603563490336434 1107 98-99 1.336038868218413 1107 100-101 1.958973797969609 1108 1 0.07641254725726654 1108 2 0.009886064338406442 1108 3 0.01871760812290546 1108 4 8.338731630175289E-5 1108 5 -0.07748597747305297 1108 6 0.021324162773304067 1108 7 0.057089693791944285 1108 8 0.04529219829034048 1108 9 0.06882412030184781 1108 10-11 0.09761572991865819 1108 12-13 0.045023748094621396 1108 14-15 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66-67 -1.1522626094457102 2316 68-69 -1.2862380891626195 2316 70-71 -1.0225176991183957 2316 72-73 -1.1002155581156075 2316 74-75 -1.1810265007698035 2316 76-77 -1.0731410950604072 2316 78-79 -1.0869820879958674 2316 80-81 -1.182553817014366 2316 82-83 -1.3042806791794774 2316 84-85 -1.4287232468530995 2316 86-87 -1.6540030029357133 2316 88-89 -2.220480561892632 2316 90-91 -2.8434000933033943 2316 92-93 -2.3593214947264833 2316 94-95 -2.2878007657460877 2316 96-97 -2.4515726524702117 2316 98-99 -2.4176793583075202 2316 100-101 -2.1847034252918647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 19.0 4 67.0 5 164.0 6 405.0 7 983.0 8 2176.0 9 3901.0 10 6268.0 11 9159.0 12 12621.0 13 17180.0 14 21299.0 15 28121.0 16 35551.0 17 45543.0 18 42611.0 19 31934.0 20 30452.0 21 31970.0 22 34815.0 23 38932.0 24 45257.0 25 53144.0 26 62891.0 27 75183.0 28 91125.0 29 107428.0 30 128117.0 31 154016.0 32 185992.0 33 231028.0 34 298008.0 35 401233.0 36 538132.0 37 633214.0 38 318990.0 39 13950.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.67465936601157 20.954253118495302 15.807339607199552 21.563747908293575 2 16.619911921187285 22.254999819126258 38.01716826751092 23.107919992175535 3 20.260217021692792 25.51340676360606 30.32639001469767 23.899986200003482 4 13.869130479636965 18.247159277416106 36.653648223350935 31.230062019595994 5 13.375196796155903 38.26695556011791 33.93961111953896 14.418236524187233 6 30.974524868580673 37.3052425439277 16.970210010994823 14.750022576496807 7 27.787163859550873 33.62357092997239 20.61183557371141 17.977429636765336 8 26.41029935273997 34.7473193841718 19.422383058427577 19.419998204660647 9 26.21104348070747 17.06713899276103 20.454649593971947 36.26716793255955 10-11 25.06585813871542 27.117033349098378 26.793135372606603 21.023973139579596 12-13 26.41383643922575 24.52432215890897 27.3942525024217 21.66758889944358 14-15 22.86583053872239 25.884479291296493 26.57561795178576 24.674072218195363 16-17 21.578856470870075 29.08588493536412 27.14034379484695 22.19491479891886 18-19 21.425285077112505 27.391103959527157 29.99000505106669 21.193605912293652 20-21 22.693062621168657 26.058962159873627 29.97380680272838 21.274168416229333 22-23 22.059656179115915 26.24705745219909 30.02368776101086 21.669598607674136 24-25 22.086642493834535 26.4990485347113 30.13161880668313 21.282690164771036 26-27 21.824962766416405 26.485390876258023 30.26179268919457 21.427853668131 28-29 21.421788184791332 27.05517452976177 30.122779774832292 21.40025751061461 30-31 21.74212227868758 26.49372904041791 30.02785455607555 21.736294124818958 32-33 21.60225998389554 26.627816773560813 29.990969711017357 21.778953531526295 34-35 22.27617352342759 26.497153074739575 29.88997516519134 21.336698236641492 36-37 22.197670287950416 26.286639351039227 29.93337879535644 21.58231156565392 38-39 21.762654250805348 26.774642174578418 30.0174046992554 21.445298875360834 40-41 21.990005407841853 26.64668516445145 29.813957377202062 21.549352050504634 42-43 21.778022293412242 26.851608561971606 29.310821875794307 22.05954726882185 44-45 22.08417933469122 26.673582060635553 29.219665408193592 22.022573196479634 46-47 22.365716043547092 27.0517874694389 28.801849368734374 21.78064711827964 48-49 22.33496417035521 27.17370799174452 28.568145996088777 21.92318184181149 50-51 21.98102594011149 27.3078655033402 28.65520087773349 22.05590767881482 52-53 22.452138798489234 27.514607229322447 28.068817769036293 21.964436203152026 54-55 22.067815594531986 27.15907644528167 28.356407016829298 22.416700943357043 56-57 22.31980404378092 27.39062139086899 28.134929715899926 22.154644849450168 58-59 22.69735171749617 27.04908247778628 28.097845812751054 22.155719991966496 60-61 22.281731687016958 27.25523513280165 28.649426041975385 21.813607138206006 62-63 22.15467766387114 27.205768173088067 28.606330761557754 22.033223401483042 64-65 21.94358971198367 27.416872709070628 28.32336996497971 22.31616761396599 66-67 22.048233177268873 27.342584829987636 27.99837446064907 22.61080753209442 68-69 22.050036168092735 27.147895008963978 28.29553357884852 22.506535244094767 70-71 22.265718315471247 27.045038017696438 28.177148223261707 22.512095443570608 72-73 22.197066629866676 26.976018821587484 28.32545751007869 22.501457038467155 74-75 21.90016838770116 27.705404055453254 27.797315229065156 22.597112327780433 76-77 22.021526115270927 27.141280899168606 28.06736954764219 22.76982343791828 78-79 22.013182161567006 27.204746640700705 28.125410441648967 22.656660756083326 80-81 22.083695809019606 27.306628237209896 27.9440584512895 22.665617502481002 82-83 22.088407436724548 27.21168651111591 28.106683051713116 22.59322300044643 84-85 21.826355849657748 27.365955810535425 27.988389245649316 22.81929909415751 86-87 21.913760547589543 27.325337817902874 27.85641867426254 22.904482960245044 88-89 21.87026542965014 27.282873637119202 27.649467416074398 23.197393517156257 90-91 21.926442120731135 27.046107200505165 27.860951737558686 23.16649894120501 92-93 22.087486248503264 27.2542305572828 27.804183241817466 22.854099952396474 94-95 21.823153973466678 26.99502006163928 27.909239152934845 23.272586811959194 96-97 21.649152484920137 26.917327604675446 28.06353258096019 23.369987329444232 98-99 21.642655119329778 27.03885569892963 27.599577693310483 23.718911488430113 100-101 21.839344572868757 26.195259651597002 28.320984475153317 23.644411300380924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2778.0 1 1824.0 2 1102.0 3 1422.0 4 1265.5 5 1144.5 6 2095.0 7 4780.0 8 7272.0 9 7889.0 10 6521.5 11 4752.5 12 4417.5 13 4689.5 14 5073.0 15 5359.0 16 5590.0 17 5989.5 18 6486.5 19 7013.0 20 7857.0 21 9049.0 22 10538.5 23 12502.0 24 14777.5 25 17650.0 26 22489.5 27 28470.5 28 33242.5 29 38871.0 30 45755.0 31 53266.5 32 62311.5 33 70179.5 34 77466.0 35 83626.0 36 90580.5 37 97963.0 38 102025.5 39 104125.5 40 106570.0 41 111878.0 42 115696.5 43 118792.0 44 123765.0 45 128589.0 46 130525.0 47 139301.5 48 160874.5 49 159905.5 50 139700.0 51 128784.0 52 122157.5 53 118826.0 54 115367.5 55 107733.0 56 98979.5 57 90275.0 58 82875.5 59 74026.5 60 62509.5 61 51605.0 62 41851.5 63 32351.5 64 24087.0 65 18508.5 66 14066.0 67 10917.0 68 8147.0 69 5781.0 70 4277.5 71 3306.5 72 2638.5 73 2205.0 74 1785.0 75 1389.0 76 1033.0 77 683.0 78 435.0 79 286.5 80 185.0 81 136.5 82 106.5 83 70.0 84 39.0 85 16.0 86 10.0 87 7.5 88 3.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2050706278462493 2 0.0 3 0.0 4 0.0 5 0.02323222714526305 6 0.0034299020468208426 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002559028480245238 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.545630425374844E-4 26-27 0.0010450482798907254 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006096114966029232 36-37 0.01515320005841552 38-39 0.011294560255742072 40-41 0.007154561300790352 42-43 0.006551648831622625 44-45 0.0030547565104498127 46-47 4.555338655933931E-4 48-49 0.0015809704747064821 50-51 1.3398054870393917E-5 52-53 0.0 54-55 0.0 56-57 8.306794019644228E-4 58-59 3.6174748150063574E-4 60-61 0.002116892669522239 62-63 7.368930178716654E-4 64-65 0.001286213267557816 66-67 0.0053726200030279605 68-69 1.2058249383354524E-4 70-71 1.2058249383354524E-4 72-73 0.0 74-75 5.627183045565445E-4 76-77 0.052265812049406675 78-79 0.13276132571073332 80-81 6.833007983900897E-4 82-83 7.234949630012715E-4 84-85 0.0 86-87 0.09499220903109286 88-89 0.3278905968431503 90-91 0.3619350542688212 92-93 4.957280302045749E-4 94-95 3.0815526201906005E-4 96-97 1.2058249383354524E-4 98-99 0.0 100-101 7.636891276124532E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3731885.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.40946551202576 #Duplication Level Percentage of deduplicated Percentage of total 1 68.25960660330378 29.631130387105607 2 15.696400160564112 13.627446828659265 3 6.251604300670073 8.141364038543083 4 3.1770985068403537 5.5166459224397935 5 1.7829110332118598 3.8697607503610216 6 1.138667320654151 2.965736387136429 7 0.7190974652994486 2.185094563379109 8 0.5004908896317503 1.7380833610042044 9 0.3723320260855456 1.454646082084485 >10 1.8531976504407073 14.563168130957834 >50 0.15514753719923036 4.668179488842509 >100 0.08652706055945128 6.736875847344864 >500 0.004386434236738069 1.2326678788379979 >1k 0.00228588824880872 2.0319723835356767 >5k 0.0 0.0 >10k+ 2.4712305392526706E-4 1.637227949768184 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19075 0.5111357933055279 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16503 0.44221619905222165 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13894 0.3723051487385061 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11576 0.31019176635935997 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4943 0.13245317044871424 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4852 0.13001472446230256 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4067 0.1089797783157841 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3900 0.10450482798907254 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3795 0.10169123646628982 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.6796109740787833E-5 0.0 5 0.0 0.0 0.0 2.6796109740787833E-5 0.0 6 0.0 0.0 0.0 2.6796109740787833E-5 0.0 7 0.0 0.0 0.0 2.6796109740787833E-5 0.0 8 0.0 0.0 0.0 2.6796109740787833E-5 0.0 9 0.0 0.0 0.0 2.6796109740787833E-5 0.0 10-11 0.0 0.0 0.0 2.6796109740787833E-5 0.0 12-13 0.0 0.0 0.0 2.6796109740787833E-5 0.0 14-15 0.0 0.0 0.0 4.019416461118175E-5 0.0 16-17 0.0 0.0 0.0 8.03883292223635E-5 0.0 18-19 0.0 0.0 0.0 1.0718443896315133E-4 0.0 20-21 0.0 0.0 0.0 2.2776693279669656E-4 0.0 22-23 0.0 0.0 0.0 4.1533970098221143E-4 0.0 24-25 0.0 0.0 0.0 7.100969081308775E-4 0.0 26-27 0.0 0.0 0.0 0.001112038554242695 0.0 28-29 0.0 0.0 0.0 0.002572426535115632 0.0 30-31 2.6796109740787833E-5 0.0 0.0 0.006632037160844989 0.0 32-33 5.3592219481575666E-5 0.0 0.0 0.015997277515250336 0.0 34-35 5.3592219481575666E-5 0.0 0.0 0.030641351488590887 0.0 36-37 5.3592219481575666E-5 0.0 0.0 0.057102509857618874 0.0 38-39 5.3592219481575666E-5 0.0 0.0 0.09722968419444866 0.0 40-41 5.3592219481575666E-5 0.0 0.0 0.14218015828462024 0.0 42-43 5.3592219481575666E-5 0.0 0.0 0.19061412664109426 0.0 44-45 5.3592219481575666E-5 0.0 0.0 0.25069100467994054 0.0 46-47 5.3592219481575666E-5 0.0 0.0 0.3277298201847056 0.0 48-49 5.3592219481575666E-5 0.0 0.0 0.4090024210285151 0.0 50-51 5.3592219481575666E-5 0.0 0.0 0.5021992907069751 0.0 52-53 8.03883292223635E-5 0.0 0.0 0.595489946769528 0.0 54-55 8.03883292223635E-5 0.0 0.0 0.6986415712166907 0.0 56-57 8.03883292223635E-5 0.0 0.0 0.8293128003676427 0.0 58-59 8.03883292223635E-5 0.0 0.0 0.975646355662085 0.0 60-61 8.03883292223635E-5 0.0 0.0 1.1364096160519415 0.0 62-63 8.03883292223635E-5 0.0 0.0 1.2993835554954132 1.3398054870393917E-5 64-65 8.03883292223635E-5 0.0 0.0 1.4708786578364552 2.6796109740787833E-5 66-67 8.03883292223635E-5 0.0 0.0 1.6731088980501811 2.6796109740787833E-5 68-69 8.03883292223635E-5 0.0 0.0 1.906770974989851 2.6796109740787833E-5 70-71 8.03883292223635E-5 0.0 0.0 2.1634777063065984 2.6796109740787833E-5 72-73 8.03883292223635E-5 0.0 0.0 2.4223013302928678 2.6796109740787833E-5 74-75 8.03883292223635E-5 0.0 0.0 2.6883331077994095 2.6796109740787833E-5 76-77 8.03883292223635E-5 0.0 0.0 2.9838674021305587 2.6796109740787833E-5 78-79 8.03883292223635E-5 0.0 0.0 3.3280366356412374 2.6796109740787833E-5 80-81 8.03883292223635E-5 0.0 0.0 3.6992431438803717 2.6796109740787833E-5 82-83 8.03883292223635E-5 0.0 0.0 4.075138971324144 2.6796109740787833E-5 84-85 8.03883292223635E-5 0.0 0.0 4.453701011687123 2.6796109740787833E-5 86-87 8.03883292223635E-5 0.0 0.0 4.845768291359461 2.6796109740787833E-5 88-89 8.03883292223635E-5 0.0 0.0 5.278699638386499 2.6796109740787833E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 17780 0.0 21.71035 12-13 GTACATG 18920 0.0 21.597164 1 GTATCAA 34910 0.0 20.929848 1 TACATGG 19530 0.0 20.755093 2 AGTACTT 18640 0.0 20.505041 12-13 GGTATCA 27415 0.0 20.31858 1 GTACTTT 19015 0.0 20.237906 14-15 ACATGGG 19530 0.0 20.025991 3 AGAGTAC 29975 0.0 19.257236 10-11 GTGTAGG 1465 0.0 19.15751 1 GTACCGT 230 2.081013E-6 18.567598 6 ACTTTTT 21590 0.0 17.922709 16-17 TACTTTT 21210 0.0 17.796741 14-15 TATCAAC 41195 0.0 17.65157 2 ATCAACG 41330 0.0 17.547974 3 TCAACGC 41425 0.0 17.542107 4 CAACGCA 41515 0.0 17.526945 5 AACGCAG 42225 0.0 17.283342 6 GTGTAGC 1940 0.0 17.164099 1 TGCACCG 725 0.0 17.021757 5 >>END_MODULE