FastQCFastQC Report
Thu 26 May 2016
SRR938675_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938675_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945718
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140430.4767258780372052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102900.34932060706422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77780.2640442839402821No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.055.8612331
GTATCAA129600.044.5817261
TATCAAC172000.033.344122
ATCAACG174250.032.8862723
TCAACGC177700.032.3013154
CAACGCA180300.031.7827665
AACGCAG186800.030.7795286
ACGCAGA213500.026.799027
CGCAGAG214650.026.5447488
GTACATG157600.025.134741
TACATGG158500.024.5728192
GTGGTAT31800.024.5246241
GCAGAGT226250.024.4053889
TGGTATC31400.023.3233782
GAGTACT123600.022.8537412-13
ACATGGG166300.022.3908163
AGTACTT129000.021.8602412-13
GTACTTT132250.021.1403414-15
AGAGTAC214150.020.92500110-11
CAGAGTA222000.020.62389210-11
Produced by FastQC (version 0.11.5)