##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938675_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2945718 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.144081341119552 31.0 31.0 34.0 28.0 34.0 2 31.186270036710916 31.0 31.0 34.0 28.0 34.0 3 31.219994581966095 31.0 31.0 34.0 27.0 34.0 4 34.88756085952559 37.0 35.0 37.0 32.0 37.0 5 34.873832797301034 37.0 35.0 37.0 32.0 37.0 6 34.896399451678676 37.0 35.0 37.0 32.0 37.0 7 34.8095415107624 37.0 35.0 37.0 32.0 37.0 8 34.78847975264435 37.0 35.0 37.0 32.0 37.0 9 36.273155814643495 39.0 35.0 39.0 32.0 39.0 10-11 36.10874869895896 39.0 35.0 39.0 31.0 39.0 12-13 35.970499043017696 38.0 35.0 39.0 30.0 39.0 14-15 36.89708875730807 39.0 36.0 41.0 31.0 41.0 16-17 36.73711876018003 39.0 36.0 40.5 30.0 41.0 18-19 36.929232193984625 39.0 36.0 41.0 31.0 41.0 20-21 36.92590431263278 39.0 36.0 41.0 30.5 41.0 22-23 36.834562744974235 39.0 36.0 41.0 30.0 41.0 24-25 36.696491992784104 39.0 36.0 41.0 30.0 41.0 26-27 36.48363998183126 39.0 36.0 41.0 30.0 41.0 28-29 36.26356664147757 39.0 35.0 40.5 29.0 41.0 30-31 35.99474134998666 38.5 35.0 40.0 28.5 41.0 32-33 35.70388967986752 38.0 35.0 40.0 27.0 41.0 34-35 35.438760600980814 38.0 34.0 40.0 27.0 41.0 36-37 35.14261531484005 38.0 34.0 40.0 25.5 41.0 38-39 34.97375716209087 38.0 34.0 40.0 25.0 41.0 40-41 34.84968316722782 38.0 34.0 40.0 25.0 41.0 42-43 34.5266476628109 38.0 33.0 40.0 24.0 41.0 44-45 34.12554070009417 38.0 33.0 40.0 23.5 41.0 46-47 33.67440960064745 38.0 33.0 40.0 21.0 41.0 48-49 33.27202009832577 38.0 32.0 40.0 18.5 41.0 50-51 32.56841354128264 36.5 31.5 39.5 15.0 40.5 52-53 32.59054464819783 37.0 31.0 39.0 15.0 40.0 54-55 32.69687543070994 37.0 31.5 40.0 13.5 41.0 56-57 32.380815814684226 37.0 31.0 40.0 9.5 41.0 58-59 32.016981768112224 36.0 31.0 39.0 8.0 41.0 60-61 31.54830401280774 36.0 30.0 39.0 6.5 40.5 62-63 30.991556897163953 35.0 29.0 39.0 2.0 40.0 64-65 30.483866412195603 35.0 28.5 38.5 2.0 40.0 66-67 29.95422491222853 34.5 28.0 38.0 2.0 40.0 68-69 29.365380189142343 34.0 27.5 37.5 2.0 40.0 70-71 28.74604018443042 34.0 26.0 37.0 2.0 39.0 72-73 28.104689756453265 33.5 26.0 36.0 2.0 39.0 74-75 27.42526049676174 33.0 25.0 36.0 2.0 38.5 76-77 26.669904247453424 32.5 23.0 35.0 2.0 37.5 78-79 25.876220330663017 32.0 20.5 35.0 2.0 37.0 80-81 25.16820126705951 31.0 18.5 35.0 2.0 36.0 82-83 24.42768045006345 31.0 14.5 34.0 2.0 36.0 84-85 23.694862848378563 30.5 7.0 34.0 2.0 35.0 86-87 22.909512214000117 30.0 2.0 34.0 2.0 35.0 88-89 22.06835073825804 29.0 2.0 34.0 2.0 35.0 90-91 21.137734671139597 29.0 2.0 34.0 2.0 35.0 92-93 20.169631987854913 27.0 2.0 33.5 2.0 35.0 94-95 19.131687758298654 25.5 2.0 33.0 2.0 35.0 96-97 17.859839604469947 24.0 2.0 32.5 2.0 34.0 98-99 16.32500548253431 10.0 2.0 32.0 2.0 34.0 100-101 12.839108495789482 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4818624434931209 1101 2 0.4265623023712841 1101 3 0.3631077381498926 1101 4 0.3313685853818029 1101 5 0.355774107397707 1101 6 0.4692187733838864 1101 7 0.46263170998935976 1101 8 0.5851150304991748 1101 9 0.6336192356740256 1101 10-11 0.6878289438775695 1101 12-13 0.7693664491187562 1101 14-15 0.8829173690274104 1101 16-17 0.7495702915899258 1101 18-19 0.65042199151668 1101 20-21 0.7803085221007606 1101 22-23 0.8239248943074031 1101 24-25 0.7074919631483851 1101 26-27 0.7178362589751188 1101 28-29 0.9080081810733631 1101 30-31 0.9100805839638824 1101 32-33 0.851668312486197 1101 34-35 0.8921820021103954 1101 36-37 0.9088248118058502 1101 38-39 0.9341071964691636 1101 40-41 0.9878576476726693 1101 42-43 1.1544097953489114 1101 44-45 0.9546113400756369 1101 46-47 0.7617457966406747 1101 48-49 0.5644858918167373 1101 50-51 0.7207495766849519 1101 52-53 0.6943197151012086 1101 54-55 0.8426302416605225 1101 56-57 0.7328250805030976 1101 58-59 0.7609159042987415 1101 60-61 0.7269275699471116 1101 62-63 0.8396458663079507 1101 64-65 0.7139434753261646 1101 66-67 0.8262235248310823 1101 68-69 0.6195260916544534 1101 70-71 0.834543868859722 1101 72-73 1.079924621372701 1101 74-75 0.9457459478531334 1101 76-77 0.9712801254097201 1101 78-79 1.2053341447120154 1101 80-81 1.2516259879670955 1101 82-83 1.2805740380045059 1101 84-85 1.3298813455493068 1101 86-87 1.3331800199684807 1101 88-89 1.3575088607733825 1101 90-91 1.017418270507548 1101 92-93 1.030927679781506 1101 94-95 0.9191120545948657 1101 96-97 1.050836166391644 1101 98-99 1.071287998042596 1101 100-101 1.0294193213689091 1102 1 0.5486947174700845 1102 2 0.47047902179609835 1102 3 0.39907544268432815 1102 4 0.4045882784620005 1102 5 0.3632925342143949 1102 6 0.42749402742200715 1102 7 0.456979255494538 1102 8 0.5289434154470882 1102 9 0.7978672331878798 1102 10-11 0.6997154051739543 1102 12-13 0.7250187336088132 1102 14-15 0.7924966616307785 1102 16-17 0.9057885559500534 1102 18-19 0.8224938804124378 1102 20-21 0.9089402489343783 1102 22-23 0.8926746180496536 1102 24-25 0.8647229799060909 1102 26-27 0.7875228449869951 1102 28-29 0.6494698956598413 1102 30-31 0.8453751376231722 1102 32-33 0.9302750486190732 1102 34-35 0.6722164382759885 1102 36-37 0.8326074586712693 1102 38-39 0.773284386997581 1102 40-41 0.8468678663191938 1102 42-43 0.8049720011131569 1102 44-45 0.8102262235169704 1102 46-47 0.831105089475237 1102 48-49 1.0029644307549788 1102 50-51 1.1670558831137825 1102 52-53 0.7969548569905811 1102 54-55 0.7783561314144691 1102 56-57 0.8991946695063646 1102 58-59 0.9449274999838835 1102 60-61 0.886686965600564 1102 62-63 0.9456466945711774 1102 64-65 1.1036073895023186 1102 66-67 0.9129277920187349 1102 68-69 1.078971930515774 1102 70-71 1.176434199464591 1102 72-73 1.1444982120161846 1102 74-75 1.1604268795064598 1102 76-77 1.1975141050105051 1102 78-79 1.3773593842997371 1102 80-81 1.6060903471477275 1102 82-83 1.7070014150281594 1102 84-85 1.6713401360537894 1102 86-87 1.7934208011199857 1102 88-89 1.6207853830470675 1102 90-91 1.6417488087358407 1102 92-93 1.827463662650466 1102 94-95 1.8570663668337808 1102 96-97 1.8560092074437158 1102 98-99 1.8384165563273704 1102 100-101 1.7570145631394887 1103 1 0.5108328929420267 1103 2 0.37255610440810116 1103 3 0.3676610365705635 1103 4 0.37860457752545784 1103 5 0.3884366940383188 1103 6 0.5070588504821245 1103 7 0.3902125129539371 1103 8 0.4735863261239004 1103 9 0.6469590427019796 1103 10-11 0.6813591121603295 1103 12-13 0.622798073383386 1103 14-15 0.7207841534460542 1103 16-17 0.7370397629914436 1103 18-19 0.8095109721828351 1103 20-21 0.5950013446249542 1103 22-23 0.7917699465598389 1103 24-25 0.8467288797249637 1103 26-27 0.7350845924316687 1103 28-29 0.8713814555040713 1103 30-31 0.8611248002305771 1103 32-33 0.8543501670265385 1103 34-35 0.9523579906975144 1103 36-37 1.0138024968739288 1103 38-39 1.0475184006229838 1103 40-41 1.098558624920834 1103 42-43 1.0932423058170428 1103 44-45 0.9498397875677753 1103 46-47 0.7101822195647429 1103 48-49 0.8076329998668328 1103 50-51 1.0891155881897419 1103 52-53 0.9630449100946166 1103 54-55 0.9939210177779145 1103 56-57 1.0071628088889781 1103 58-59 0.9485365617783614 1103 60-61 1.0535764805748293 1103 62-63 1.0287687273080017 1103 64-65 1.2359888697786339 1103 66-67 1.2511020931055832 1103 68-69 1.2919778569683729 1103 70-71 1.3581538061141707 1103 72-73 1.4282886481439583 1103 74-75 1.380882591428957 1103 76-77 1.5406246505089385 1103 78-79 1.3662601361297106 1103 80-81 1.5302346231564243 1103 82-83 1.452765656701228 1103 84-85 1.5857099018687855 1103 86-87 1.4847417987959055 1103 88-89 1.5288876045436162 1103 90-91 1.6446331632020623 1103 92-93 1.736453031422343 1103 94-95 1.6957154428946595 1103 96-97 1.9597697983674358 1103 98-99 2.0113032898150784 1103 100-101 1.637249294974616 1104 1 0.4781359230895852 1104 2 0.3546972731698794 1104 3 0.4395109616029558 1104 4 0.44843439604203184 1104 5 0.4730961001951641 1104 6 0.5629552234277213 1104 7 0.5867936625517629 1104 8 0.6658995624696473 1104 9 0.7249329309593193 1104 10-11 0.7427576092900168 1104 12-13 0.5949194551914587 1104 14-15 0.8797473087652179 1104 16-17 0.884888240221791 1104 18-19 0.8952048629517222 1104 20-21 0.8862803162408497 1104 22-23 0.979961185916018 1104 24-25 0.9619870299636233 1104 26-27 1.0171836659702862 1104 28-29 1.0187822281700747 1104 30-31 0.9784465447953963 1104 32-33 1.0180101890978293 1104 34-35 0.8768367050023969 1104 36-37 0.945929385509217 1104 38-39 1.022685333957881 1104 40-41 1.0887300743915418 1104 42-43 1.1381289944181816 1104 44-45 1.0069941309372652 1104 46-47 0.9850431990519368 1104 48-49 0.8965582426106593 1104 50-51 1.061103880196221 1104 52-53 1.1553140478461614 1104 54-55 1.1934942753154232 1104 56-57 1.2178516606910748 1104 58-59 1.2257110683444026 1104 60-61 1.1773476750799254 1104 62-63 1.2209258473008866 1104 64-65 1.3535571877849577 1104 66-67 1.2830546395978395 1104 68-69 1.4460397161797651 1104 70-71 1.5925258018210933 1104 72-73 1.4235156777237314 1104 74-75 1.4634621564091503 1104 76-77 1.4254386949217235 1104 78-79 1.723209304987261 1104 80-81 1.6532147304149518 1104 82-83 1.9024574673846217 1104 84-85 1.8567093226013789 1104 86-87 2.00682786508159 1104 88-89 1.9522946374998043 1104 90-91 1.8504425238799485 1104 92-93 1.9337988824042753 1104 94-95 2.2270484713246965 1104 96-97 2.069794935787691 1104 98-99 2.201771348140536 1104 100-101 1.854502057611061 1105 1 0.34737872011337956 1105 2 0.3104551959560915 1105 3 0.21723038059089106 1105 4 0.1907626145447665 1105 5 0.37574876260984524 1105 6 0.3680420510903488 1105 7 0.34434725588975823 1105 8 0.3019034838088075 1105 9 0.4601292254702827 1105 10-11 0.5200160015781066 1105 12-13 0.5151590497573082 1105 14-15 0.590754504078923 1105 16-17 0.5413817856157905 1105 18-19 0.5295536856676222 1105 20-21 0.5605206612666862 1105 22-23 0.6132907808812504 1105 24-25 0.6116730302626934 1105 26-27 0.6281691973247447 1105 28-29 0.5961117655505035 1105 30-31 0.7705007478821244 1105 32-33 0.6551663869406283 1105 34-35 0.7947823115143038 1105 36-37 0.8537747805363054 1105 38-39 0.8081279660290832 1105 40-41 0.7481379215280342 1105 42-43 0.8408058981763276 1105 44-45 0.958876341227672 1105 46-47 0.8176787810717272 1105 48-49 0.6500747466931429 1105 50-51 0.8421725054891311 1105 52-53 0.8235295831108189 1105 54-55 0.7983081324475307 1105 56-57 0.7982039357086492 1105 58-59 0.8695644017148894 1105 60-61 0.938066110482314 1105 62-63 1.0295252317007986 1105 64-65 1.153631931927329 1105 66-67 1.150014710111357 1105 68-69 1.0637009048600738 1105 70-71 1.001186671927428 1105 72-73 0.9979943030816649 1105 74-75 1.0710693407631773 1105 76-77 1.296499012213971 1105 78-79 1.283828104752832 1105 80-81 1.3340788367870857 1105 82-83 1.498773735183665 1105 84-85 1.391469180051132 1105 86-87 1.5616772715401908 1105 88-89 1.4618750765003163 1105 90-91 1.6535872577034958 1105 92-93 1.7026772930266247 1105 94-95 1.6032345324593216 1105 96-97 1.729340823907858 1105 98-99 1.8080288887308562 1105 100-101 1.39126554104568 1106 1 0.20992345988923944 1106 2 0.13344704011498365 1106 3 0.14711948462187152 1106 4 0.07867260691700295 1106 5 0.33137570622305645 1106 6 0.14952711827335463 1106 7 0.31649417837993354 1106 8 0.27260993220404117 1106 9 0.06397244556063697 1106 10-11 0.1438571680394105 1106 12-13 0.25692253036518053 1106 14-15 0.5811757914109705 1106 16-17 0.32575513537815937 1106 18-19 0.2547575816953156 1106 20-21 0.45371581470211453 1106 22-23 0.3890770274340838 1106 24-25 0.13255110337188825 1106 26-27 0.18548451739750504 1106 28-29 0.4134898679972636 1106 30-31 0.10835939952796281 1106 32-33 0.3241375187836084 1106 34-35 0.08802587192033684 1106 36-37 0.20943252858536 1106 38-39 0.265035494046451 1106 40-41 0.3394320012088414 1106 42-43 -0.1175699746160177 1106 44-45 0.12682359229848572 1106 46-47 0.28544267240541643 1106 48-49 0.5184503261099138 1106 50-51 0.18696215519869952 1106 52-53 0.1412362662065192 1106 54-55 0.2733301356159856 1106 56-57 -0.4849577729107253 1106 58-59 -0.14917056278666507 1106 60-61 0.31660862060377326 1106 62-63 0.5353061974932025 1106 64-65 0.4876444297270126 1106 66-67 0.11005988995058757 1106 68-69 0.18201487540516936 1106 70-71 0.06072871264795765 1106 72-73 0.39266220192576284 1106 74-75 0.8043029263995081 1106 76-77 0.8565603277100706 1106 78-79 1.2745853071233064 1106 80-81 1.2037672719217412 1106 82-83 0.9387476247570952 1106 84-85 0.8340497116476833 1106 86-87 0.7375808683247911 1106 88-89 0.7757077350698189 1106 90-91 1.0903442840486228 1106 92-93 0.8498770231210919 1106 94-95 0.9725870525555464 1106 96-97 0.9830696275222444 1106 98-99 1.226639168611161 1106 100-101 0.7210989037165714 1107 1 0.2173797501538992 1107 2 0.020916915984095397 1107 3 0.10621383534680007 1107 4 0.02466290662236048 1107 5 0.09571522518881892 1107 6 0.21854912781788727 1107 7 0.18164537231227484 1107 8 0.1803835804580345 1107 9 0.35985652660698975 1107 10-11 0.3760028065844594 1107 12-13 0.2124015527208556 1107 14-15 0.3659191359790981 1107 16-17 0.4340000613279642 1107 18-19 0.43600003714189484 1107 20-21 0.523248906898182 1107 22-23 0.383154850449138 1107 24-25 0.5383380733061642 1107 26-27 0.2957321301818183 1107 28-29 0.47791527048160987 1107 30-31 0.3757881291874128 1107 32-33 0.5293283878729014 1107 34-35 0.5396098495603354 1107 36-37 0.5834410823242067 1107 38-39 0.4662363489876711 1107 40-41 0.3982283555940924 1107 42-43 0.7539985786036425 1107 44-45 0.4687501088889334 1107 46-47 0.5504083622642355 1107 48-49 0.5645650163018914 1107 50-51 0.5378249925132437 1107 52-53 0.6411445417433441 1107 54-55 0.5894119867194902 1107 56-57 0.6067837804344052 1107 58-59 0.5860007932210749 1107 60-61 0.4952209203350897 1107 62-63 0.7591561804030356 1107 64-65 0.649679587886812 1107 66-67 0.8042525848758864 1107 68-69 0.8826396401449017 1107 70-71 0.9994567712477753 1107 72-73 1.100035529144133 1107 74-75 0.9437576417724429 1107 76-77 1.0537343921854472 1107 78-79 1.279034051752884 1107 80-81 1.178642448772564 1107 82-83 1.0694375701118162 1107 84-85 1.1659793434085763 1107 86-87 1.3627000668606613 1107 88-89 1.2543797422347076 1107 90-91 1.0860388588927528 1107 92-93 1.3648486620901465 1107 94-95 1.505346639276265 1107 96-97 1.675965957245726 1107 98-99 1.7305899186317113 1107 100-101 1.5115862764596955 1108 1 0.4340138622405121 1108 2 0.3049530703166674 1108 3 0.2632743442549952 1108 4 0.22799864319956242 1108 5 0.21089835748483665 1108 6 0.401513537651077 1108 7 0.39742556583306055 1108 8 0.4731268066339567 1108 9 0.4775784048187717 1108 10-11 0.5935379220679948 1108 12-13 0.5069732043289008 1108 14-15 0.6696778690434826 1108 16-17 0.6636187210678273 1108 18-19 0.6406803545746129 1108 20-21 0.6838490872266902 1108 22-23 0.6088601428693678 1108 24-25 0.8027598801023998 1108 26-27 0.8456725178028961 1108 28-29 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90-91 -3.7533218690459655 2316 92-93 -3.938760880123553 2316 94-95 -4.121171929454533 2316 96-97 -4.231355975789537 2316 98-99 -4.214476677609719 2316 100-101 -3.6230144328735605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10708.0 3 3262.0 4 3567.0 5 4054.0 6 4949.0 7 6159.0 8 7644.0 9 9877.0 10 12584.0 11 16044.0 12 19887.0 13 24264.0 14 28087.0 15 33008.0 16 39234.0 17 45417.0 18 41608.0 19 37321.0 20 39081.0 21 42529.0 22 46820.0 23 52433.0 24 57974.0 25 64792.0 26 73074.0 27 83121.0 28 93946.0 29 107568.0 30 123328.0 31 143161.0 32 167454.0 33 198303.0 34 239242.0 35 287059.0 36 329277.0 37 309998.0 38 133353.0 39 5531.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.421672218048265 19.04354182106818 15.184689390269199 22.350096570614355 2 15.394162161574338 20.5731605697826 40.30706219950003 23.72561506914303 3 18.88661255579716 25.25872141829297 29.836076828281026 26.018589197628838 4 12.697488025389948 17.15510523008279 37.23489404598563 32.91251269854163 5 13.809584362985037 37.733975174117106 33.4043719339953 15.052068528902558 6 31.265276136826614 36.32972431680648 17.80141200520438 14.603587541162529 7 28.380677361321172 33.259046663076276 20.439926951629563 17.920349023972985 8 26.61757639863969 34.18030741124956 20.008760864065685 19.193355326045065 9 27.199701190991586 16.655308528790076 20.056909533874926 36.08808074634341 10-11 24.89529037211466 26.926698760127284 27.104742913514933 21.07326795424313 12-13 26.15330217158008 24.41455378306549 27.49214913009933 21.939994915255102 14-15 23.032405186826576 25.631506305767278 26.470809456469162 24.86527905093699 16-17 22.013419354575806 28.768040630587755 26.893365251997903 22.325174762838536 18-19 21.926432433166703 26.918248071071503 29.895623913279724 21.25969558248207 20-21 23.066450633324163 26.02208227609469 29.786768976796797 21.124698113784355 22-23 22.512790934002634 26.03623417380321 29.676512002754723 21.774462889439434 24-25 22.103377939706252 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28.303607698002235 22.451816190004603 50-51 22.48563820812096 27.11614268462724 27.995283635416733 22.402935471835068 52-53 22.658921779422812 27.075140548382286 27.86570504038973 22.400232631805167 54-55 22.258684128998308 27.092801880907015 28.064371835624346 22.58414215447034 56-57 22.327227934639364 27.278795513989373 27.898004593436397 22.495971957934866 58-59 22.286429848838747 26.848244204930644 28.0705489361601 22.794777010070515 60-61 22.301751045166966 26.9396540015158 28.222411395104352 22.53618355821288 62-63 22.157846865568978 26.992251304492193 28.18392346843331 22.66597836150552 64-65 21.969088301992105 26.873034365324607 28.185921731264223 22.971955601419065 66-67 22.042499115577847 26.991155099466084 28.07549739833898 22.89084838661708 68-69 22.334588749600645 26.759769821866243 28.108122881633584 22.797518546899525 70-71 22.200587102589804 26.832904300308293 27.997957756391305 22.968550840710602 72-73 22.312685968479272 26.813621912551838 27.834815421641746 23.038876697327147 74-75 22.234265842312563 27.071692252001885 27.662842704096064 23.031199201589487 76-77 22.281812400106894 26.83265971097679 27.776540285084362 23.108987603831956 78-79 22.37092323844265 26.9622901412539 27.673592574643557 22.993194045659898 80-81 22.340768634310027 27.113177402949766 27.563511937175196 22.982542025565014 82-83 22.508672380581203 27.03312920675811 27.396540825201882 23.06165758745881 84-85 22.513622151230493 26.807680686590373 27.437915242211186 23.24078191996795 86-87 22.585961944155994 26.776409905920545 27.329590797905635 23.30803735201783 88-89 22.575376370323166 26.696775952915953 27.324669696952718 23.403177979808166 90-91 22.42586168545488 26.786544479592756 27.388056026746675 23.399537808205686 92-93 22.636607992860732 26.39035968161943 27.49019853537059 23.482833790149247 94-95 22.458820296900743 26.449343165947266 27.456186439728143 23.635650097423845 96-97 22.70814590514189 26.216784667889982 27.41540811713927 23.659661309828863 98-99 22.64952156968743 26.20799451806028 27.101186678316015 24.041297233936277 100-101 23.339325001392886 25.6075354370192 27.347434986268198 23.705704575319718 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1396.0 1 949.0 2 641.0 3 780.0 4 667.5 5 633.5 6 1065.5 7 2411.5 8 3843.5 9 4208.0 10 3612.0 11 2971.0 12 2950.5 13 3214.0 14 3548.5 15 3927.5 16 4165.0 17 4471.0 18 4951.5 19 5270.0 20 5758.5 21 6625.0 22 7736.0 23 9206.0 24 10830.0 25 13023.0 26 16486.5 27 20857.5 28 24783.0 29 29011.0 30 34137.0 31 39708.5 32 46418.5 33 51463.5 34 55328.5 35 59868.5 36 65623.5 37 72182.0 38 76164.0 39 79129.0 40 83379.0 41 89791.5 42 96350.0 43 100448.0 44 102724.5 45 106427.0 46 108441.5 47 109580.5 48 112505.5 49 112735.0 50 110061.0 51 105583.5 52 101982.5 53 99911.0 54 97795.0 55 91955.0 56 82490.5 57 74845.5 58 68947.5 59 60440.0 60 50669.0 61 42233.0 62 34697.0 63 27042.5 64 20482.0 65 16021.5 66 12630.5 67 10049.0 68 7760.0 69 5903.5 70 4676.0 71 3759.0 72 3160.5 73 2822.5 74 2274.5 75 1787.5 76 1383.5 77 925.0 78 603.5 79 409.0 80 255.0 81 170.5 82 114.5 83 61.5 84 32.0 85 19.5 86 13.0 87 10.0 88 7.0 89 6.0 90 5.0 91 4.0 92 3.0 93 3.0 94 3.0 95 2.5 96 2.0 97 1.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006959254076595248 2 0.1059843474494164 3 0.11419966201788494 4 0.11715310155283024 5 0.15595518647745643 6 0.0954266498015085 7 0.027837016306380993 8 0.027259907431736508 9 0.02352567353697808 10-11 0.05107413540603683 12-13 0.05491021204337957 14-15 0.02832925622887187 16-17 0.031961647381045975 18-19 0.036595492168632573 20-21 0.05866141972856872 22-23 0.03247086109396759 24-25 0.050327288627085146 26-27 0.05721864754195752 28-29 0.05329770195246117 30-31 0.06730107905780526 32-33 0.05031031483665443 34-35 0.061648806844375466 36-37 0.052601776544801644 38-39 0.032538756255690464 40-41 0.03724049620499993 42-43 0.03318376029205783 44-45 0.006874385124441647 46-47 0.012407840804856405 48-49 0.008571764167513658 50-51 0.007773996017269812 52-53 0.00987874603067911 54-55 0.011219675474705997 56-57 0.01454653839912714 58-59 0.019859334803942537 60-61 0.02491752435229713 62-63 0.010829278294799434 64-65 0.008945187556989502 66-67 0.009912693611540548 68-69 0.011779810558919761 70-71 0.031146905440371415 72-73 0.028193465905426117 74-75 0.029432552606868684 76-77 0.02359356869870096 78-79 0.004599897206725152 80-81 0.0044131855119872295 82-83 0.005431612937830437 84-85 0.005024241967493155 86-87 0.01318863516466953 88-89 0.03773273612749082 90-91 0.035746802647096565 92-93 0.029789002205913802 94-95 0.0464063430375888 96-97 0.04267210914283037 98-99 0.02630937516761618 100-101 0.07420941176310834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2945718.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.57599523175742 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6864342435393 33.6297745852056 2 14.530597765230974 13.826152999864277 3 5.895947728822462 8.415167430994455 4 3.010682738962732 5.729449105329012 5 1.6893737849874337 4.018681956960906 6 1.0152939235632743 2.8982171319767174 7 0.6736529882964108 2.243479795133443 8 0.4644085010631195 1.7675757305733266 9 0.32585583536836815 1.395262410275323 >10 1.5290830975746366 12.677685002798253 >50 0.10104800606884569 3.3721420736273573 >100 0.07122815624139633 6.274118021696317 >500 0.004381876934296936 1.4062301324913282 >1k 0.0017958512024223486 1.2486456878322205 >5k 7.183404809689393E-5 0.26581908692683953 >10k+ 1.4366809619378786E-4 0.8315988483145779 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14043 0.4767258780372052 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10290 0.34932060706422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7778 0.2640442839402821 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.394758086144023E-5 0.0 0.0 0.0 0.0 3 3.394758086144023E-5 0.0 0.0 0.0 0.0 4 3.394758086144023E-5 0.0 0.0 0.0 0.0 5 3.394758086144023E-5 0.0 0.0 0.0 0.0 6 1.018427425843207E-4 0.0 0.0 0.0 0.0 7 2.7158064689152185E-4 0.0 0.0 0.0 0.0 8 2.7158064689152185E-4 0.0 0.0 0.0 0.0 9 2.7158064689152185E-4 0.0 0.0 0.0 0.0 10-11 3.055282277529621E-4 0.0 0.0 0.0 0.0 12-13 3.734233894758426E-4 0.0 0.0 0.0 0.0 14-15 4.41318551198723E-4 0.0 0.0 0.0 0.0 16-17 4.5829234162944317E-4 0.0 0.0 3.394758086144023E-5 0.0 18-19 4.922399224908834E-4 0.0 0.0 6.789516172288046E-5 0.0 20-21 5.092137129216035E-4 0.0 0.0 1.1881653301504082E-4 0.0 22-23 5.092137129216035E-4 0.0 0.0 2.88554437322242E-4 0.0 24-25 7.128991980902449E-4 0.0 0.0 7.468467789516851E-4 1.6973790430720115E-5 26-27 7.298729885209651E-4 0.0 0.0 0.0018161955760870525 3.394758086144023E-5 28-29 0.001171191539719688 0.0 0.0 0.005431612937830437 3.394758086144023E-5 30-31 0.0013579032344576093 0.0 0.0 0.01753392551493388 3.394758086144023E-5 32-33 0.0013748770248883293 0.0 0.0 0.0410935466327734 3.394758086144023E-5 34-35 0.0014936935579033701 0.0 0.0 0.07314006296597297 3.394758086144023E-5 36-37 0.0017992217856563322 0.0 0.0 0.12626802701412695 3.394758086144023E-5 38-39 0.0021556713847014548 0.0 0.0 0.2023615295150452 3.394758086144023E-5 40-41 0.002223566546424335 0.0 0.0 0.2871456127164922 3.394758086144023E-5 42-43 0.002495147193315857 0.0 0.0 0.37467944996771585 3.394758086144023E-5 44-45 0.0028685705827916994 0.0 0.0 0.4830401280774331 6.789516172288046E-5 46-47 0.003275941553128983 0.0 0.0 0.6117014595422916 6.789516172288046E-5 48-49 0.003530548409589784 0.0 0.0 0.7487308696894951 6.789516172288046E-5 50-51 0.003530548409589784 0.0 0.0 0.9033960480942167 6.789516172288046E-5 52-53 0.003530548409589784 0.0 0.0 1.050864339356313 8.486895215360058E-5 54-55 0.003530548409589784 0.0 0.0 1.2163587960558342 1.018427425843207E-4 56-57 0.0037002863138969853 0.0 0.0 1.4118629142368686 1.018427425843207E-4 58-59 0.0037002863138969853 0.0 0.0 1.619723951851467 1.018427425843207E-4 60-61 0.0037512076851891458 0.0 0.0 1.8457808928077977 1.018427425843207E-4 62-63 0.0037681814756198658 0.0 0.0 2.0764207571804225 1.018427425843207E-4 64-65 0.003802129056481306 0.0 0.0 2.320503863574178 1.3579032344576092E-4 66-67 0.004073709703372828 0.0 0.0 2.5999264016446926 1.3579032344576092E-4 68-69 0.004107657284234268 0.0 0.0 2.8977485285421074 1.3579032344576092E-4 70-71 0.00422647381724931 0.0 0.0 3.2293824459775173 1.3579032344576092E-4 72-73 0.00446410688327939 0.0 0.0 3.570114315083793 1.3579032344576092E-4 74-75 0.004633844787586591 0.0 0.0 3.9193500531958594 1.3579032344576092E-4 76-77 0.004684766158878752 0.0 0.0 4.297271497135842 1.3579032344576092E-4 78-79 0.004786608901463073 0.0 0.0 4.707850513864532 1.8671169473792127E-4 80-81 0.0052279274526617955 0.0 0.0 5.146351415851755 2.036854851686414E-4 82-83 0.005516481889984038 0.0 0.0 5.605390604260149 2.036854851686414E-4 84-85 0.005652272213429799 0.0 0.0 6.06833376446761 2.036854851686414E-4 86-87 0.00577108874644484 0.0 0.0 6.5497953300349865 2.036854851686414E-4 88-89 0.00577108874644484 0.0 0.0 7.0520497888799945 2.036854851686414E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6955 0.0 55.861233 1 GTATCAA 12960 0.0 44.581726 1 TATCAAC 17200 0.0 33.34412 2 ATCAACG 17425 0.0 32.886272 3 TCAACGC 17770 0.0 32.301315 4 CAACGCA 18030 0.0 31.782766 5 AACGCAG 18680 0.0 30.779528 6 ACGCAGA 21350 0.0 26.79902 7 CGCAGAG 21465 0.0 26.544748 8 GTACATG 15760 0.0 25.13474 1 TACATGG 15850 0.0 24.572819 2 GTGGTAT 3180 0.0 24.524624 1 GCAGAGT 22625 0.0 24.405388 9 TGGTATC 3140 0.0 23.323378 2 GAGTACT 12360 0.0 22.85374 12-13 ACATGGG 16630 0.0 22.390816 3 AGTACTT 12900 0.0 21.86024 12-13 GTACTTT 13225 0.0 21.14034 14-15 AGAGTAC 21415 0.0 20.925001 10-11 CAGAGTA 22200 0.0 20.623892 10-11 >>END_MODULE