FastQCFastQC Report
Thu 26 May 2016
SRR938675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945718
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137020.465149752963454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110470.3750189257763303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80900.2746359291690515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67550.056.7160721
GTATCAA127900.043.259241
TATCAAC176200.031.3734952
ATCAACG176450.031.2317773
TCAACGC179250.030.7439144
CAACGCA182650.030.1716255
AACGCAG190200.028.9097676
TGGTATC30300.027.2957922
GTGGTAT33300.026.1218131
ACGCAGA216650.025.3583627
CGCAGAG219250.024.9926768
GTACATG160150.024.1598321
TACATGG160300.023.8402922
GCAGAGT232300.023.241169
ACATGGG166550.022.0778473
AGTACTT133700.021.87740712-13
GAGTACT127950.021.59878212-13
AGAGTAC218200.021.48962610-11
TAAGGTG13000.020.0992435
CAGAGTA226950.019.9497310-11
Produced by FastQC (version 0.11.5)