##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938675_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2945718 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800436090623744 31.0 31.0 34.0 30.0 34.0 2 32.01742868801426 31.0 31.0 34.0 30.0 34.0 3 32.06997580895388 33.0 31.0 34.0 30.0 34.0 4 35.75552921223281 37.0 35.0 37.0 35.0 37.0 5 35.6521028150013 37.0 35.0 37.0 33.0 37.0 6 35.65676551523262 37.0 35.0 37.0 33.0 37.0 7 35.596444398275736 37.0 35.0 37.0 33.0 37.0 8 35.5781853524336 37.0 35.0 37.0 33.0 37.0 9 37.15486648755923 39.0 37.0 39.0 33.0 39.0 10-11 37.05953523045994 39.0 37.0 39.0 33.0 39.0 12-13 36.95173502691024 39.0 37.0 39.0 33.0 39.0 14-15 37.96367422136131 40.0 37.0 41.0 33.0 41.0 16-17 37.886115711008316 40.0 37.0 41.0 33.0 41.0 18-19 38.15238118516436 40.0 37.5 41.0 33.0 41.0 20-21 38.13720305202331 40.0 37.0 41.0 33.0 41.0 22-23 38.02624911821159 40.0 37.0 41.0 33.0 41.0 24-25 37.89988366164039 40.0 37.0 41.0 32.5 41.0 26-27 37.70142304864213 40.0 37.0 41.0 32.0 41.0 28-29 37.58232678756079 39.5 37.0 41.0 32.0 41.0 30-31 37.30864885912365 39.0 36.0 41.0 31.5 41.0 32-33 37.0873518782178 39.0 36.0 41.0 31.0 41.0 34-35 36.96390353727003 39.0 35.5 41.0 31.0 41.0 36-37 36.90252087945961 39.0 35.0 41.0 31.0 41.0 38-39 36.73236151593601 39.0 35.0 41.0 30.0 41.0 40-41 36.545104962525265 39.0 35.0 41.0 30.0 41.0 42-43 36.307396363127765 39.0 35.0 40.0 30.0 41.0 44-45 35.96226760334832 38.5 35.0 40.0 29.5 41.0 46-47 35.52062790803464 38.0 35.0 40.0 27.5 41.0 48-49 35.12318949064371 38.0 34.0 40.0 26.5 41.0 50-51 34.743872801130316 38.0 34.0 40.0 25.5 41.0 52-53 34.27066355299455 38.0 33.0 40.0 24.0 41.0 54-55 33.93984675383048 37.5 33.0 40.0 23.0 41.0 56-57 33.61369520096628 37.0 33.0 40.0 23.0 41.0 58-59 33.15722889971138 36.5 32.0 39.5 21.5 41.0 60-61 32.77224092733928 36.0 31.0 39.0 20.0 40.0 62-63 32.302606868681934 35.5 31.0 39.0 18.5 40.0 64-65 31.795500621580207 35.0 30.5 38.5 16.0 40.0 66-67 31.30285417680851 35.0 30.0 38.0 13.0 40.0 68-69 30.819058545319002 34.5 29.0 38.0 9.0 40.0 70-71 30.26781755755303 34.0 29.0 37.0 7.5 39.0 72-73 29.782197243592222 34.0 28.5 36.5 7.0 39.0 74-75 29.200434495087446 34.0 28.0 36.0 2.0 39.0 76-77 27.967575307616002 31.5 26.0 34.5 2.0 36.5 78-79 28.661431610222024 33.0 27.5 35.0 2.0 37.0 80-81 28.29767139963839 33.0 27.0 35.0 2.0 37.0 82-83 27.865302449182167 33.0 26.0 35.0 2.0 36.0 84-85 27.31364458512322 33.0 26.0 35.0 2.0 36.0 86-87 26.715060980039503 32.0 25.0 35.0 2.0 35.5 88-89 25.983882707034414 32.0 23.5 34.0 2.0 35.0 90-91 25.386228416976778 31.0 21.0 34.0 2.0 35.0 92-93 24.576515131455217 31.0 18.5 34.0 2.0 35.0 94-95 23.77276881222167 31.0 10.0 34.0 2.0 35.0 96-97 22.71663445041243 30.0 2.0 34.0 2.0 35.0 98-99 21.363897868024026 29.5 2.0 34.0 2.0 35.0 100-101 17.437657134864914 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2238948344139544 1101 2 0.2563409272736479 1101 3 0.24201359897480756 1101 4 0.23442865014202852 1101 5 0.06273755886254406 1101 6 0.13617169755009684 1101 7 0.1948697592692028 1101 8 0.19721442204338047 1101 9 0.28142264721831367 1101 10-11 0.21246906578279123 1101 12-13 0.33869253186359316 1101 14-15 0.41584571199739884 1101 16-17 0.3850447780608519 1101 18-19 0.39832243879146034 1101 20-21 0.3224679112725468 1101 22-23 0.29668502456036805 1101 24-25 0.42029601826523333 1101 26-27 0.3212753901038994 1101 28-29 0.3323864405977872 1101 30-31 0.42897398509989415 1101 32-33 0.38223040785660345 1101 34-35 0.3601496637311996 1101 36-37 0.3841890574711826 1101 38-39 0.49976953864150886 1101 40-41 0.5307845390400558 1101 42-43 0.6165424965903412 1101 44-45 0.3884078135411144 1101 46-47 0.3977684799590122 1101 48-49 0.5697944361428924 1101 50-51 0.5811326229331897 1101 52-53 0.567709811256556 1101 54-55 0.5019467069721344 1101 56-57 0.5942857523897658 1101 58-59 0.6821489933153657 1101 60-61 0.7127623445126972 1101 62-63 0.6965833476130641 1101 64-65 0.5234458366463919 1101 66-67 0.43337791728534825 1101 68-69 0.6070938606957945 1101 70-71 0.4526302050843789 1101 72-73 0.5544505679413447 1101 74-75 0.5538954854432774 1101 76-77 0.4304619823649496 1101 78-79 0.5810769222138674 1101 80-81 0.7603919673273758 1101 82-83 0.43288911447307044 1101 84-85 0.5688849043987254 1101 86-87 0.7639276023968691 1101 88-89 0.6569782157051485 1101 90-91 0.6223748019094977 1101 92-93 0.9172056161667292 1101 94-95 0.9074107437892458 1101 96-97 1.0855555462754722 1101 98-99 1.1735093149292446 1101 100-101 1.7050937255195713 1102 1 0.3371333776227061 1102 2 0.37045043890435636 1102 3 0.27963402185146435 1102 4 0.2272819217477675 1102 5 0.17234928837543606 1102 6 0.19055622249217663 1102 7 0.2247866541389385 1102 8 0.27441154665075373 1102 9 0.32833716270132385 1102 10-11 0.3835663513506731 1102 12-13 0.4081292788699855 1102 14-15 0.5812931951448306 1102 16-17 0.5781834536693111 1102 18-19 0.4465466029970173 1102 20-21 0.44153625794898943 1102 22-23 0.4730730624809496 1102 24-25 0.4308931634091877 1102 26-27 0.4371875642812597 1102 28-29 0.38431324062526073 1102 30-31 0.4877260685083158 1102 32-33 0.4690857752811084 1102 34-35 0.4224147936454443 1102 36-37 0.5290068021607439 1102 38-39 0.6190444071088237 1102 40-41 0.5546417839972051 1102 42-43 0.4798226229650808 1102 44-45 0.5950246710777947 1102 46-47 0.7471386802994289 1102 48-49 0.7394613016017999 1102 50-51 0.8478110085951229 1102 52-53 0.8966510147679045 1102 54-55 0.8520082826987192 1102 56-57 0.9217702870561197 1102 58-59 0.9022787817546316 1102 60-61 0.9014857243162666 1102 62-63 0.9970820055954377 1102 64-65 1.0333749310582085 1102 66-67 0.9754614413073632 1102 68-69 1.0106612407251312 1102 70-71 0.980234559110464 1102 72-73 1.0126973291805292 1102 74-75 1.2051238378862763 1102 76-77 1.0588101412473243 1102 78-79 1.2355097965116606 1102 80-81 1.2153024870183486 1102 82-83 1.1501352140537016 1102 84-85 1.1318424297277367 1102 86-87 1.1104897319989817 1102 88-89 1.0059361204847974 1102 90-91 1.276007701776006 1102 92-93 1.712571218218212 1102 94-95 1.6142549997021796 1102 96-97 1.589316935744833 1102 98-99 1.7261697875288995 1102 100-101 1.7756994253033973 1103 1 0.2543656891347865 1103 2 0.249127419896503 1103 3 0.26678267389583254 1103 4 0.12809435308070505 1103 5 0.08642222333268279 1103 6 0.1364554212032587 1103 7 0.1879822010842105 1103 8 0.1222893990158056 1103 9 0.30073716820820096 1103 10-11 0.2581028185076377 1103 12-13 0.33218020820017813 1103 14-15 0.4642085399363154 1103 16-17 0.42336145761495914 1103 18-19 0.406553888026572 1103 20-21 0.40293473658469026 1103 22-23 0.3655960385660606 1103 24-25 0.5014601643869057 1103 26-27 0.5009476559835946 1103 28-29 0.5213283445167036 1103 30-31 0.28277478312261906 1103 32-33 0.5101859125283781 1103 34-35 0.49687276210005393 1103 36-37 0.5281098119896157 1103 38-39 0.5763149167765462 1103 40-41 0.5894970863523028 1103 42-43 0.5881361643579339 1103 44-45 0.6158585431970849 1103 46-47 0.6235771423218779 1103 48-49 0.7286092838145919 1103 50-51 0.6712706951175491 1103 52-53 0.6140183733837077 1103 54-55 0.5327989901816252 1103 56-57 0.4388069887055934 1103 58-59 0.48164668256602994 1103 60-61 0.5447155554186125 1103 62-63 0.5910595944153485 1103 64-65 0.5561913425236718 1103 66-67 0.5986189058957727 1103 68-69 0.603911227247437 1103 70-71 0.4757020664247662 1103 72-73 0.4856284007322067 1103 74-75 0.6239916784179869 1103 76-77 0.6204222943544053 1103 78-79 0.7252744071968245 1103 80-81 0.773934044011348 1103 82-83 0.9528895007634901 1103 84-85 0.8818720549141226 1103 86-87 0.8707640104021372 1103 88-89 0.9243774355317065 1103 90-91 1.0438610742802048 1103 92-93 1.3586359963098005 1103 94-95 1.2037002245016986 1103 96-97 1.3029114416333947 1103 98-99 1.4595617665080773 1103 100-101 1.8493575458918663 1104 1 0.2150849579727634 1104 2 0.23290658879558634 1104 3 0.26132629742235025 1104 4 0.21334243271518716 1104 5 0.13422180120774385 1104 6 0.16501994966224487 1104 7 0.15980405449071355 1104 8 0.20437435123174197 1104 9 0.2786710134761279 1104 10-11 0.3120862775036457 1104 12-13 0.4125705406156257 1104 14-15 0.42569574731282955 1104 16-17 0.4102423701553519 1104 18-19 0.39058958614396033 1104 20-21 0.37047133047768455 1104 22-23 0.345521100273551 1104 24-25 0.3559692517512332 1104 26-27 0.2960111054318233 1104 28-29 0.38112315534594643 1104 30-31 0.36255921795576995 1104 32-33 0.49164923336996225 1104 34-35 0.5563111927666853 1104 36-37 0.5013295664007629 1104 38-39 0.41974664494548364 1104 40-41 0.502772558909669 1104 42-43 0.4663043918216587 1104 44-45 0.43195115855156274 1104 46-47 0.3575303235407077 1104 48-49 0.5157398115000831 1104 50-51 0.6171843190422948 1104 52-53 0.5264662948984764 1104 54-55 0.6134130527224713 1104 56-57 0.5318569158879427 1104 58-59 0.6820665476023464 1104 60-61 0.8320890529208711 1104 62-63 0.7897554609478377 1104 64-65 0.8180638812174443 1104 66-67 0.6489009536634676 1104 68-69 0.662696424583153 1104 70-71 0.5898597063168651 1104 72-73 0.7007582008519684 1104 74-75 0.841237905667203 1104 76-77 0.6497666105586717 1104 78-79 0.8115002296233946 1104 80-81 0.7184396889978792 1104 82-83 1.049782479949684 1104 84-85 1.099121885788076 1104 86-87 1.0024482151163596 1104 88-89 1.3083551946927905 1104 90-91 1.2670477242065097 1104 92-93 1.6329961330374339 1104 94-95 1.613333470786113 1104 96-97 1.6463853749665809 1104 98-99 1.811420784890469 1104 100-101 2.1685445144030275 1105 1 0.11856749166684111 1105 2 0.22386832926832057 1105 3 0.173222249117984 1105 4 0.09269368420937951 1105 5 0.06882195371458977 1105 6 0.07597782638061545 1105 7 0.08696021053808778 1105 8 0.11852214726638266 1105 9 0.16805409450444841 1105 10-11 0.1967742236117047 1105 12-13 0.20956577500898277 1105 14-15 0.33568130002939256 1105 16-17 0.23956322709148736 1105 18-19 0.2386280574346884 1105 20-21 0.20562021040687029 1105 22-23 0.20960181465944316 1105 24-25 0.22797762848214376 1105 26-27 0.1183121894192567 1105 28-29 0.1418035104769828 1105 30-31 0.2746415075645956 1105 32-33 0.3570238642931898 1105 34-35 0.3923375443144579 1105 36-37 0.4004788438508271 1105 38-39 0.2587109823069582 1105 40-41 0.3861044758426644 1105 42-43 0.42899106642016704 1105 44-45 0.2879387703891396 1105 46-47 0.31216069877999075 1105 48-49 0.42006022726233994 1105 50-51 0.4667329981677142 1105 52-53 0.41472356985568126 1105 54-55 0.44442720465161045 1105 56-57 0.37163672832867434 1105 58-59 0.3987843904385713 1105 60-61 0.45000519366183767 1105 62-63 0.330666327106826 1105 64-65 0.2918364426172353 1105 66-67 0.26339997054231645 1105 68-69 0.5067811652611489 1105 70-71 0.5061463829587076 1105 72-73 0.5505690301381136 1105 74-75 0.5414929970459212 1105 76-77 0.529249053231613 1105 78-79 0.8007915464856161 1105 80-81 0.7391829880311036 1105 82-83 0.8489320360358903 1105 84-85 0.751021766253178 1105 86-87 0.7036827124985763 1105 88-89 0.6490127109488846 1105 90-91 0.8105911213196499 1105 92-93 1.1461809626003543 1105 94-95 1.3076724204950771 1105 96-97 1.5171295563475553 1105 98-99 1.7346733648288541 1105 100-101 1.7541416502093483 1106 1 0.026937496947599726 1106 2 0.1023316279833324 1106 3 0.11493145524367065 1106 4 0.047463971130696336 1106 5 -0.051863958390086395 1106 6 0.01572141612420097 1106 7 0.10208078790122954 1106 8 0.14723481522389648 1106 9 -0.17217063118938825 1106 10-11 0.0990258811837208 1106 12-13 0.1081517047161995 1106 14-15 0.144583781986789 1106 16-17 0.18117415239806434 1106 18-19 0.1667701098902441 1106 20-21 0.16532214081818353 1106 22-23 0.17523139865286907 1106 24-25 0.21166154563287876 1106 26-27 0.22066359468828267 1106 28-29 0.2932967921762213 1106 30-31 0.22331691682352783 1106 32-33 0.13921303286679887 1106 34-35 0.07772450670371 1106 36-37 0.26924901342191276 1106 38-39 0.11349007049566495 1106 40-41 0.21646444767861084 1106 42-43 0.2807483861408713 1106 44-45 0.16904244931890133 1106 46-47 -0.3125708625648471 1106 48-49 0.0016934491129561025 1106 50-51 0.21430824900383527 1106 52-53 0.3680401807809872 1106 54-55 0.5787433461759903 1106 56-57 0.4873235205600892 1106 58-59 0.2580931975734657 1106 60-61 0.441316875877412 1106 62-63 0.09113836841411427 1106 64-65 0.21929786372853854 1106 66-67 0.20657312080929202 1106 68-69 0.14145229123662162 1106 70-71 0.156215326219872 1106 72-73 0.5880462935307058 1106 74-75 0.5301129583203448 1106 76-77 0.43800338052140475 1106 78-79 0.6944018263659189 1106 80-81 0.12205278794895946 1106 82-83 0.489080484079075 1106 84-85 0.7254188794374734 1106 86-87 0.8682224062144712 1106 88-89 1.0261763558470847 1106 90-91 0.8913486170657059 1106 92-93 1.100760555395052 1106 94-95 1.3187004085253555 1106 96-97 1.4632657999666385 1106 98-99 1.6988947460410841 1106 100-101 1.471909942176726 1107 1 0.10400594849155809 1107 2 0.06685611019441495 1107 3 0.07523920812411689 1107 4 0.024946697077901092 1107 5 0.006466093918817251 1107 6 0.08588760181432065 1107 7 0.028558222385299814 1107 8 0.11194079937979495 1107 9 0.0701487430074792 1107 10-11 0.16400671423222235 1107 12-13 0.09271095136413976 1107 14-15 0.2510106598504791 1107 16-17 0.15300403380526006 1107 18-19 0.09309773496216422 1107 20-21 0.14877528562602294 1107 22-23 0.17374942121189463 1107 24-25 0.09290832770507507 1107 26-27 0.16708482630553334 1107 28-29 0.1326795773981928 1107 30-31 0.1310084673563523 1107 32-33 0.18468630081039095 1107 34-35 0.23863192989529125 1107 36-37 0.4230273474114554 1107 38-39 0.22037448901880907 1107 40-41 0.2980530331748952 1107 42-43 0.26655651327443763 1107 44-45 0.31106296185097193 1107 46-47 0.16966812292797329 1107 48-49 0.10779864236168635 1107 50-51 0.30200267674268844 1107 52-53 0.37831862077285194 1107 54-55 0.19597553886658403 1107 56-57 0.18760907785109993 1107 58-59 0.21178402555980824 1107 60-61 0.30659300112456833 1107 62-63 0.3697356331213868 1107 64-65 0.2724941584063636 1107 66-67 0.2981948266247052 1107 68-69 0.3232347095607473 1107 70-71 0.4693067218594962 1107 72-73 0.50091605777288 1107 74-75 0.43035795349615924 1107 76-77 0.2882730374632594 1107 78-79 0.5455985710127997 1107 80-81 0.5082636157622389 1107 82-83 0.605607004130821 1107 84-85 0.6942283434983558 1107 86-87 1.0084223828506929 1107 88-89 0.9859874121061978 1107 90-91 1.126176114502183 1107 92-93 1.2406145101472035 1107 94-95 1.2629121406033903 1107 96-97 1.22564860249512 1107 98-99 1.0990977729483689 1107 100-101 1.3676514609088493 1108 1 0.18365686453653396 1108 2 0.13700782135517642 1108 3 0.16550913843004622 1108 4 0.15047400457550708 1108 5 0.15142984925154934 1108 6 0.19189451838425953 1108 7 0.17975890688369844 1108 8 0.20111137344828478 1108 9 0.19772903436245315 1108 10-11 0.2246922438066079 1108 12-13 0.3316973619575023 1108 14-15 0.4185779038069981 1108 16-17 0.43606418989679696 1108 18-19 0.393049627474106 1108 20-21 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-1.2448039198031395 2316 74-75 -1.2792124374974776 2316 76-77 -1.136437843235889 2316 78-79 -1.1843299417405468 2316 80-81 -1.1753110333523296 2316 82-83 -1.336018210120745 2316 84-85 -1.56008472758543 2316 86-87 -1.6474719266513347 2316 88-89 -1.5979254431447991 2316 90-91 -1.5143001849436253 2316 92-93 -1.643109943189664 2316 94-95 -1.7879226400615416 2316 96-97 -1.929550064637212 2316 98-99 -1.8758207495830597 2316 100-101 -2.3353915496875413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 19.0 4 58.0 5 122.0 6 283.0 7 705.0 8 1530.0 9 2619.0 10 4468.0 11 6944.0 12 9700.0 13 13425.0 14 17157.0 15 23178.0 16 29672.0 17 36972.0 18 34025.0 19 26902.0 20 25816.0 21 27384.0 22 29845.0 23 33641.0 24 38761.0 25 45743.0 26 54145.0 27 64567.0 28 77468.0 29 92144.0 30 108754.0 31 129945.0 32 156507.0 33 192777.0 34 243734.0 35 317380.0 36 413055.0 37 461546.0 38 217133.0 39 7586.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.12161167585066 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21.841890500041078 27.006420845444133 29.94468581174437 21.20700284277042 20-21 22.908217962479778 26.126075204754834 29.830282464241314 21.135424368524074 22-23 22.448160346645537 26.096286202548917 29.77751094979221 21.678042501013337 24-25 22.11124259069361 26.41876154607541 29.904952766653125 21.565043096577856 26-27 21.78884619517164 26.731730782777436 29.78588658529154 21.693536436759388 28-29 21.76488929935335 26.760519391814785 29.931702478295165 21.5428888305367 30-31 21.88943009832072 26.537445182176118 29.726248664987814 21.846876054515345 32-33 21.807973433881916 26.55888722130205 29.497570532303452 22.13556881251258 34-35 22.442419641456635 26.58977062921298 29.33693719142163 21.63087253790875 36-37 22.409181585894856 26.253291567983293 29.446354445208446 21.891172400913405 38-39 21.96797263148496 26.575766459140652 29.34896061871801 22.107300290656372 40-41 22.180298361942423 26.410453395544124 29.445351232515097 21.963897009998355 42-43 22.31761987845597 26.307426651523595 29.069741586115132 22.305211883905304 44-45 22.068705854873052 26.725552581685196 29.0512599094983 22.154481653943456 46-47 22.313269634092606 26.920737151349854 28.429673173060017 22.336320041497522 48-49 22.278592180242644 26.906479167388053 28.420778906874318 22.39414974549499 50-51 22.434836895554188 27.10321079388649 28.067716907788952 22.394235402770374 52-53 22.653373472952946 27.078287874127803 27.871303363051048 22.39703528986821 54-55 22.243571801151756 27.150989408703985 28.018227410471663 22.587211379672596 56-57 22.326526164418997 27.29568818196447 27.908204383447433 22.469581270169105 58-59 22.31590266952207 26.903773404879157 28.01156105870884 22.768762866889933 60-61 22.301965089665746 27.05888683166549 28.10061927177007 22.538528806898693 62-63 22.094591509195503 27.13103181586004 28.1228722359544 22.65150443899005 64-65 21.96521380413221 26.95423436331551 28.03136546556309 23.04918636698919 66-67 22.038455917163933 27.06390381216817 27.951163942226326 22.946476328441566 68-69 22.249150790911642 26.85514137130737 28.003977943397153 22.891729894383836 70-71 22.164539595035706 26.89613885110804 27.87680009260905 23.062521461247204 72-73 22.230635568629907 26.934160161033827 27.709726988323553 23.125477282012714 74-75 22.15273826931987 27.21823048853761 27.439177913950026 23.189853328192495 76-77 22.110393436932473 26.944674555308634 27.824258474108536 23.12067353365036 78-79 22.181399078686226 27.078257677515733 27.660552024209316 23.079791219588724 80-81 22.030427825935774 27.308148632092514 27.607272280280455 23.054151261691256 82-83 22.263075765834145 27.212450312684126 27.4251400413969 23.09933388008483 84-85 22.17521056358364 27.04914233950559 27.444049326194836 23.331597770715938 86-87 22.29398501882069 26.997425502363154 27.326953051660723 23.381636427155435 88-89 22.137510660202025 26.99494426115881 27.300038393715663 23.5675066849235 90-91 21.949941612923052 27.000483191686524 27.54795329441314 23.50162190097728 92-93 21.997799995891334 26.78412472417181 27.52241133217736 23.695663947759495 94-95 21.828659288910938 26.79644548849745 27.52452644516421 23.850368777427402 96-97 21.988805934668118 26.677513655523544 27.45157570002812 23.882104709780222 98-99 21.825392714481286 26.713959232112572 27.22104861648782 24.23959943691833 100-101 21.879764485351124 26.545715547290772 27.52909768445906 24.045422282899047 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1482.0 1 984.0 2 608.5 3 749.0 4 684.5 5 687.0 6 1143.5 7 2488.0 8 3809.0 9 4191.5 10 3609.5 11 2907.0 12 2992.0 13 3277.5 14 3562.0 15 3981.5 16 4261.5 17 4490.5 18 4943.5 19 5320.0 20 5848.0 21 6684.5 22 7712.5 23 9180.5 24 10858.5 25 13161.5 26 16588.0 27 20891.0 28 24983.5 29 29335.5 30 34205.0 31 39748.5 32 47058.0 33 51967.5 34 55753.0 35 60178.5 36 65717.0 37 72352.0 38 76719.0 39 79893.5 40 84106.5 41 90674.0 42 97009.5 43 101398.5 44 104392.5 45 108326.5 46 110181.0 47 111307.0 48 114452.0 49 114358.0 50 111550.5 51 107082.5 52 102173.0 53 100109.0 54 99226.0 55 92344.0 56 81388.5 57 73286.5 58 67168.0 59 58500.5 60 48176.5 61 39853.0 62 33020.5 63 25294.0 64 18637.5 65 14652.0 66 11482.0 67 9115.0 68 7029.0 69 5221.5 70 4029.5 71 3276.5 72 2843.0 73 2533.5 74 2096.5 75 1788.0 76 1277.5 77 746.0 78 506.0 79 329.5 80 208.5 81 144.5 82 80.0 83 36.5 84 19.5 85 8.5 86 4.5 87 3.0 88 3.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.752661320601633E-4 2 0.05149848016680483 3 0.0 4 0.0 5 0.04464106883279391 6 3.394758086144024E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006195433507212842 26-27 0.002019881061255694 28-29 1.1881653301504082E-4 30-31 0.0017652742047948923 32-33 3.734233894758426E-4 34-35 0.012085338786672723 36-37 0.02352567353697808 38-39 0.004871477853616674 40-41 0.001154217749288968 42-43 0.0012390867014425684 44-45 0.008435973844067898 46-47 0.0 48-49 0.0 50-51 4.582923416294431E-4 52-53 0.0 54-55 0.0015106673483340903 56-57 0.0 58-59 0.022371455787689114 60-61 0.0 62-63 0.009658086755079747 64-65 0.014953909369464424 66-67 0.006433066573242924 68-69 0.01620996986133771 70-71 5.092137129216035E-5 72-73 5.092137129216035E-5 74-75 0.022863695710179998 76-77 0.050412157579238746 78-79 0.03060374414658837 80-81 0.026547008233646266 82-83 0.035746802647096565 84-85 0.01587049405272331 86-87 0.009810850868956228 88-89 0.08581948441772091 90-91 0.024340415477652647 92-93 0.02456107475325201 94-95 0.02663187718579986 96-97 0.03808918572653594 98-99 0.05304309509600036 100-101 0.014376800494819939 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2945718.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.63343480263367 #Duplication Level Percentage of deduplicated Percentage of total 1 67.78974960488797 30.256893692766297 2 15.819577170701981 14.12164132507518 3 6.399847342895814 8.569415073778462 4 3.3430377607912565 5.968450317560761 5 1.8477992975540696 4.123681473786593 6 1.1877127996285142 3.180702108388369 7 0.7759824382505234 2.424433309595041 8 0.5334029872743312 1.9046085964831123 9 0.35882183472509355 1.4413905869367476 >10 1.739351316884048 13.539140241012406 >50 0.11724629677530427 3.665338329826378 >100 0.07968740579386042 6.680690608904645 >500 0.005341784986308632 1.5720538844095726 >1k 0.0022130252084976818 1.4330537348046275 >5k 7.631121408612695E-5 0.27554795632859813 >10k+ 1.526224281722539E-4 0.8429587603431984 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13702 0.465149752963454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11047 0.3750189257763303 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8090 0.2746359291690515 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.789516172288046E-5 0.0 0.0 0.0 0.0 6 1.018427425843207E-4 0.0 0.0 0.0 0.0 7 2.3763306603008164E-4 0.0 0.0 0.0 0.0 8 2.3763306603008164E-4 0.0 0.0 0.0 0.0 9 2.3763306603008164E-4 0.0 0.0 0.0 0.0 10-11 2.7158064689152185E-4 0.0 0.0 0.0 0.0 12-13 3.903971799065627E-4 0.0 0.0 0.0 0.0 14-15 4.41318551198723E-4 0.0 0.0 0.0 0.0 16-17 4.5829234162944317E-4 0.0 0.0 3.394758086144023E-5 0.0 18-19 5.77108874644484E-4 0.0 0.0 8.486895215360058E-5 0.0 20-21 6.789516172288046E-4 0.0 0.0 1.5276411387648106E-4 0.0 22-23 6.959254076595248E-4 0.0 0.0 3.5644959904512243E-4 0.0 24-25 0.0010863225875660874 0.0 0.0 7.977681502438455E-4 0.0 26-27 0.001171191539719688 1.6973790430720115E-5 0.0 0.0018671169473792127 0.0 28-29 0.0014257983961804897 3.394758086144023E-5 0.0 0.005516481889984038 0.0 30-31 0.0017313266239334518 3.394758086144023E-5 0.0 0.01766971583837964 0.0 32-33 0.0017483004143641718 3.394758086144023E-5 0.0 0.041399074860526366 0.0 34-35 0.0018501431569484927 3.394758086144023E-5 0.0 0.07353046014587955 0.0 36-37 0.0019689596899635335 3.394758086144023E-5 0.0 0.1269979000026479 0.0 38-39 0.002342383079439376 3.394758086144023E-5 0.0 0.20400798718682508 0.0 40-41 0.002342383079439376 3.394758086144023E-5 0.0 0.2893012841011937 0.0 42-43 0.002512120983746577 3.394758086144023E-5 0.0 0.3776159157122304 0.0 44-45 0.0029534395349453003 3.394758086144023E-5 0.0 0.48704594261908307 0.0 46-47 0.003241993972267542 3.394758086144023E-5 0.0 0.6170142559471069 0.0 48-49 0.0035984435713126645 3.394758086144023E-5 0.0 0.7546377487593856 0.0 50-51 0.0035984435713126645 3.394758086144023E-5 0.0 0.9098970098291825 0.0 52-53 0.0036154173617433845 3.394758086144023E-5 0.0 1.0581630692415227 0.0 54-55 0.0036663387330355453 3.394758086144023E-5 0.0 1.2249645078042093 0.0 56-57 0.003937919379927067 3.394758086144023E-5 0.0 1.4211984989737647 0.0 58-59 0.003971866960788507 3.394758086144023E-5 0.0 1.629263222073532 0.0 60-61 0.004005814541649948 3.394758086144023E-5 0.0 1.8554899009341694 0.0 62-63 0.004005814541649948 3.394758086144023E-5 0.0 2.0866729266005777 0.0 64-65 0.004039762122511388 3.394758086144023E-5 0.0 2.33163867009673 0.0 66-67 0.00437923793112579 3.394758086144023E-5 0.0 2.6134884601988375 0.0 68-69 0.00437923793112579 3.394758086144023E-5 0.0 2.9133983633192315 0.0 70-71 0.004532002045002271 3.394758086144023E-5 0.0 3.2483251960982007 0.0 72-73 0.004667792368448031 3.394758086144023E-5 0.0 3.5944038091901533 0.0 74-75 0.004718713739740192 3.394758086144023E-5 0.0 3.9496652429051253 0.0 76-77 0.004718713739740192 3.394758086144023E-5 0.0 4.335683184880562 0.0 78-79 0.004786608901463072 3.394758086144023E-5 0.0 4.757481877083957 0.0 80-81 0.005499508099553318 3.394758086144023E-5 0.0 5.210071025128679 0.0 82-83 0.005567403261276198 3.394758086144023E-5 0.0 5.684132018068261 0.0 84-85 0.005703193584721959 3.394758086144023E-5 0.0 6.1642187066107486 0.0 86-87 0.00588990527945988 3.394758086144023E-5 0.0 6.663061433579182 0.0 88-89 0.0059408266507520405 3.394758086144023E-5 0.0 7.189893940967872 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6755 0.0 56.716072 1 GTATCAA 12790 0.0 43.25924 1 TATCAAC 17620 0.0 31.373495 2 ATCAACG 17645 0.0 31.231777 3 TCAACGC 17925 0.0 30.743914 4 CAACGCA 18265 0.0 30.171625 5 AACGCAG 19020 0.0 28.909767 6 TGGTATC 3030 0.0 27.295792 2 GTGGTAT 3330 0.0 26.121813 1 ACGCAGA 21665 0.0 25.358362 7 CGCAGAG 21925 0.0 24.992676 8 GTACATG 16015 0.0 24.159832 1 TACATGG 16030 0.0 23.840292 2 GCAGAGT 23230 0.0 23.24116 9 ACATGGG 16655 0.0 22.077847 3 AGTACTT 13370 0.0 21.877407 12-13 GAGTACT 12795 0.0 21.598782 12-13 AGAGTAC 21820 0.0 21.489626 10-11 TAAGGTG 1300 0.0 20.099243 5 CAGAGTA 22695 0.0 19.94973 10-11 >>END_MODULE