FastQCFastQC Report
Thu 26 May 2016
SRR938674_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938674_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919676
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142200.4870403428325609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103440.3542858865161751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78860.2700984629801389No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65250.054.1995931
GTATCAA125700.045.0987171
TATCAAC170350.033.1458132
ATCAACG171300.032.934813
TCAACGC174750.032.3117944
CAACGCA178700.031.5954065
AACGCAG184750.030.5539426
GTACATG162000.026.8546121
ACGCAGA211050.026.6536667
CGCAGAG211950.026.405998
TACATGG163600.026.1755522
TGGTATC31100.025.82742
GTGGTAT33550.024.543771
GCAGAGT222800.024.35039
ACATGGG169350.024.276873
AGTACTT125000.021.98263512-13
GAGTACT119100.021.89510212-13
CATGGGA98950.021.7112314
AGAGTAC210300.021.30062510-11
CATGGGG66750.020.7206554
Produced by FastQC (version 0.11.5)