##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938674_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2919676 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.072969055470537 31.0 31.0 34.0 28.0 34.0 2 31.162601261235835 31.0 31.0 34.0 27.0 34.0 3 31.224432779527593 31.0 31.0 34.0 27.0 34.0 4 34.87046610651319 37.0 35.0 37.0 32.0 37.0 5 34.89462906158081 37.0 35.0 37.0 32.0 37.0 6 34.931252303337764 37.0 35.0 37.0 32.0 37.0 7 34.85676766874133 37.0 35.0 37.0 32.0 37.0 8 34.82233028596324 37.0 35.0 37.0 32.0 37.0 9 36.320115314164994 39.0 35.0 39.0 32.0 39.0 10-11 36.17087872078957 39.0 35.0 39.0 31.5 39.0 12-13 36.02216513065149 38.0 35.0 39.0 30.0 39.0 14-15 36.9743389334981 39.0 36.0 41.0 31.0 41.0 16-17 36.84233815669958 39.0 36.0 41.0 31.0 41.0 18-19 36.86898152397732 39.0 36.0 41.0 30.5 41.0 20-21 36.9760019604915 39.0 36.0 41.0 31.0 41.0 22-23 36.887470733053945 39.0 36.0 41.0 30.0 41.0 24-25 36.769185690467026 39.0 36.0 41.0 30.0 41.0 26-27 36.580115053862144 39.0 36.0 41.0 30.0 41.0 28-29 36.385475648667864 39.0 35.5 41.0 30.0 41.0 30-31 36.14623489044675 39.0 35.0 40.0 29.0 41.0 32-33 35.84816037806935 38.0 35.0 40.0 28.0 41.0 34-35 35.58467069633754 38.0 35.0 40.0 27.0 41.0 36-37 35.28042323874293 38.0 34.0 40.0 26.0 41.0 38-39 35.013612811832544 38.0 34.0 40.0 25.0 41.0 40-41 34.909444917860746 38.0 34.0 40.0 25.0 41.0 42-43 34.70248907755518 38.0 33.5 40.0 24.0 41.0 44-45 34.35770150523551 38.0 33.0 40.0 23.5 41.0 46-47 33.930368129888386 38.0 33.0 40.0 22.5 41.0 48-49 33.52346835744788 38.0 33.0 40.0 19.5 41.0 50-51 32.77552577751778 37.0 31.5 39.5 18.0 40.5 52-53 32.839662688599695 37.0 31.5 39.5 18.0 40.0 54-55 32.965016323729074 37.0 32.0 40.0 16.0 41.0 56-57 32.67569021357164 37.0 31.5 40.0 13.5 41.0 58-59 32.3526762901089 36.5 31.0 40.0 9.5 41.0 60-61 31.913656686563854 36.0 31.0 39.0 8.0 41.0 62-63 31.40139642206875 35.5 30.0 39.0 6.5 40.0 64-65 30.917183105248665 35.0 29.0 39.0 2.0 40.0 66-67 30.3851555789067 35.0 29.0 38.0 2.0 40.0 68-69 29.830483587904958 34.5 28.0 38.0 2.0 40.0 70-71 29.22402828259026 34.0 27.5 37.0 2.0 39.0 72-73 28.678457815182234 34.0 26.0 36.5 2.0 39.0 74-75 28.06932190421129 33.5 26.0 36.0 2.0 39.0 76-77 27.331624810424174 33.0 25.0 35.5 2.0 38.0 78-79 26.542196634147075 32.0 23.0 35.0 2.0 37.0 80-81 25.88493791776896 32.0 21.0 35.0 2.0 36.5 82-83 25.216264064916793 31.0 19.0 35.0 2.0 36.0 84-85 24.54722613057065 31.0 16.5 34.0 2.0 35.5 86-87 23.861567345143776 30.5 8.5 34.0 2.0 35.0 88-89 23.059947576374913 30.0 3.5 34.0 2.0 35.0 90-91 22.22181570831832 29.0 2.0 34.0 2.0 35.0 92-93 21.357567072510783 29.0 2.0 34.0 2.0 35.0 94-95 20.386786410546925 28.0 2.0 34.0 2.0 35.0 96-97 19.138814204041818 25.5 2.0 33.0 2.0 35.0 98-99 17.648006491131206 22.5 2.0 32.5 2.0 34.0 100-101 14.26142969288373 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6732359428568202 1101 2 0.6326973079754588 1101 3 0.6716127750641654 1101 4 0.5499925587688068 1101 5 0.6808318121735439 1101 6 0.5222633401609542 1101 7 0.5250968448782274 1101 8 0.5571440709968414 1101 9 0.6779066982551925 1101 10-11 0.7150624520886666 1101 12-13 0.7718140362825707 1101 14-15 0.9328557353941846 1101 16-17 0.8326335316272306 1101 18-19 0.7741291575393703 1101 20-21 0.9276006865577173 1101 22-23 0.9327732353865699 1101 24-25 1.0177125403963814 1101 26-27 1.0567105703496722 1101 28-29 0.9804403187049431 1101 30-31 0.9048519800228476 1101 32-33 0.9912156831295178 1101 34-35 0.9333943604375321 1101 36-37 1.0286478286731864 1101 38-39 1.0832315995339243 1101 40-41 0.891569205726789 1101 42-43 1.0038290375296341 1101 44-45 1.058480461287786 1101 46-47 1.13158376428553 1101 48-49 1.2018338349373252 1101 50-51 1.0045472879371928 1101 52-53 1.0458777864415012 1101 54-55 1.193716597284073 1101 56-57 1.1580947611602923 1101 58-59 1.1803879798217523 1101 60-61 1.2641219429938495 1101 62-63 1.156877858893214 1101 64-65 1.0167305455655935 1101 66-67 1.026120738319591 1101 68-69 1.032429399902668 1101 70-71 1.179005851582854 1101 72-73 1.2591604227744106 1101 74-75 1.2135443402498183 1101 76-77 1.3379872459540572 1101 78-79 1.1958104398207858 1101 80-81 1.320177479047917 1101 82-83 1.3554692096121066 1101 84-85 1.5008107404411781 1101 86-87 1.597277172193376 1101 88-89 1.5967002600198086 1101 90-91 1.6009832235396786 1101 92-93 1.8488277531121682 1101 94-95 1.7962450730895867 1101 96-97 1.6695852041709323 1101 98-99 1.655048776100454 1101 100-101 1.5096773426367402 1102 1 0.6504253052000735 1102 2 0.7342827426651368 1102 3 0.70365308465189 1102 4 0.7537221875633406 1102 5 0.6551558306734506 1102 6 0.6818154101617537 1102 7 0.6077433639751675 1102 8 0.7200106318298651 1102 9 0.7254647376181467 1102 10-11 0.7608091079024533 1102 12-13 0.8645037765733647 1102 14-15 0.9178532812705953 1102 16-17 0.9179513847407392 1102 18-19 1.0514844389203049 1102 20-21 0.9091625983373532 1102 22-23 0.9716759913819502 1102 24-25 0.8814058067619399 1102 26-27 0.8394552682826983 1102 28-29 0.8083349168406428 1102 30-31 0.7350696947524185 1102 32-33 0.716704976190492 1102 34-35 0.9225202937234371 1102 36-37 0.9981054166524501 1102 38-39 1.0244990664963396 1102 40-41 1.0380479447365403 1102 42-43 1.1714231725840918 1102 44-45 1.1419316383988232 1102 46-47 1.1677664233218437 1102 48-49 0.9422964771747431 1102 50-51 1.1181691117295856 1102 52-53 1.1392162405784205 1102 54-55 1.1058395232440006 1102 56-57 1.2509123826150983 1102 58-59 1.5139452614835989 1102 60-61 1.4163083774766712 1102 62-63 1.2065739657349077 1102 64-65 1.3143294638340421 1102 66-67 1.2856439951173328 1102 68-69 1.245691836641086 1102 70-71 1.1903225357750031 1102 72-73 1.1820004988001465 1102 74-75 1.1858731069544248 1102 76-77 1.336386543323119 1102 78-79 1.419428472329134 1102 80-81 1.2908401785505212 1102 82-83 1.4705100211386117 1102 84-85 1.3355819554122625 1102 86-87 1.3443901906625833 1102 88-89 1.6151944742480566 1102 90-91 1.607191885310879 1102 92-93 1.712458294496141 1102 94-95 1.665515097397428 1102 96-97 1.5464275855307648 1102 98-99 1.4301574889824415 1102 100-101 1.232024343347625 1103 1 0.5933902874598971 1103 2 0.7042650785974516 1103 3 0.6304307920557726 1103 4 0.6236411869921881 1103 5 0.5841494553282303 1103 6 0.5835114650983968 1103 7 0.607565068739703 1103 8 0.5209914541719627 1103 9 0.5990204568647002 1103 10-11 0.5970680317643016 1103 12-13 0.5059404099569278 1103 14-15 0.784583681534798 1103 16-17 0.7866502464706002 1103 18-19 0.9094355526698834 1103 20-21 0.7674697730991156 1103 22-23 0.7567298340281283 1103 24-25 0.7538333155857018 1103 26-27 0.7827944455170268 1103 28-29 0.8265358557033764 1103 30-31 0.9430841780966333 1103 32-33 0.9973487583261615 1103 34-35 0.9615712521430595 1103 36-37 1.060585123491137 1103 38-39 0.9447026830967218 1103 40-41 0.9836535363504453 1103 42-43 0.8942307997021786 1103 44-45 1.035573897678745 1103 46-47 0.9861976105602963 1103 48-49 0.9682927696398096 1103 50-51 1.1508783455564497 1103 52-53 1.1715377782325334 1103 54-55 1.36468092188872 1103 56-57 1.3167616311476706 1103 58-59 1.1606375671904345 1103 60-61 1.3134377844762462 1103 62-63 1.430074389632825 1103 64-65 1.4507922954985872 1103 66-67 1.5382035641908551 1103 68-69 1.3841573460432812 1103 70-71 1.4284132545615904 1103 72-73 1.3944524421454112 1103 74-75 1.2676321277734068 1103 76-77 1.1833975831999695 1103 78-79 1.1705512227085286 1103 80-81 1.1905045678827229 1103 82-83 1.115309114705223 1103 84-85 1.2599612808420986 1103 86-87 1.2350494131391692 1103 88-89 1.1078510798747452 1103 90-91 1.3028904300273112 1103 92-93 1.454211676579387 1103 94-95 1.2935623145291224 1103 96-97 1.2292976755453715 1103 98-99 1.123113966811026 1103 100-101 0.7901650033311967 1104 1 0.45625000013822614 1104 2 0.3515809679940567 1104 3 0.4615974196886832 1104 4 0.47217542790188816 1104 5 0.4178525712186243 1104 6 0.3541337462311347 1104 7 0.3821498575338609 1104 8 0.3212781031322578 1104 9 0.4622540215768538 1104 10-11 0.440933108677406 1104 12-13 0.4759917601096575 1104 14-15 0.5349771542601616 1104 16-17 0.44555012986763387 1104 18-19 0.6037033832069127 1104 20-21 0.6502750087716507 1104 22-23 0.6421283645317644 1104 24-25 0.6585607810657379 1104 26-27 0.5683367365161729 1104 28-29 0.6399372863320849 1104 30-31 0.5351566712574183 1104 32-33 0.5386476979367885 1104 34-35 0.540501307803531 1104 36-37 0.5767568711902129 1104 38-39 0.6172358327222511 1104 40-41 0.7111095486286345 1104 42-43 0.7060918503248104 1104 44-45 0.6240865122262136 1104 46-47 0.6652063358506766 1104 48-49 0.8632138757734253 1104 50-51 0.6041994123241068 1104 52-53 0.6496722927107328 1104 54-55 0.8822339039846767 1104 56-57 0.8833444167626539 1104 58-59 0.8695463577585514 1104 60-61 0.8529949049809282 1104 62-63 0.8579892146536316 1104 64-65 0.9281264726254719 1104 66-67 0.9064062091846488 1104 68-69 0.819265303448315 1104 70-71 0.7960636549482736 1104 72-73 0.7457749390434749 1104 74-75 0.8161537865253585 1104 76-77 0.8428768687327306 1104 78-79 0.9619173709736835 1104 80-81 0.9143110692455814 1104 82-83 0.9197768396019512 1104 84-85 1.0037903553434226 1104 86-87 1.0386622364757052 1104 88-89 1.229350686215156 1104 90-91 1.2678176652912363 1104 92-93 1.082895570420856 1104 94-95 0.8124803361654536 1104 96-97 0.9825503501882125 1104 98-99 0.9383473471509696 1104 100-101 0.6628748014685399 1105 1 0.5645138061624841 1105 2 0.5807542888420123 1105 3 0.5902497951484733 1105 4 0.6481552630077232 1105 5 0.5190505844893849 1105 6 0.5133343819996554 1105 7 0.46361859844112985 1105 8 0.37653004109838406 1105 9 0.5751134776857967 1105 10-11 0.521682783353107 1105 12-13 0.5937019366709535 1105 14-15 0.7075728939097345 1105 16-17 0.6623673468923528 1105 18-19 0.7055582648722662 1105 20-21 0.6890218325354383 1105 22-23 0.6821484076366389 1105 24-25 0.8605788224665147 1105 26-27 0.7433170819996846 1105 28-29 0.6805470834036456 1105 30-31 0.7715894866940474 1105 32-33 0.697385522562108 1105 34-35 0.7428333001909948 1105 36-37 0.7855252142208826 1105 38-39 0.6966025236835875 1105 40-41 0.6524848292889729 1105 42-43 0.6499664770193121 1105 44-45 0.5543031455153482 1105 46-47 0.40924008238300047 1105 48-49 0.3998081804892806 1105 50-51 0.4026849364587548 1105 52-53 0.39779255560500815 1105 54-55 0.5137679940860096 1105 56-57 0.5447104850347841 1105 58-59 0.6205297944153187 1105 60-61 0.6116665274132878 1105 62-63 0.5987857118610087 1105 64-65 0.624548900430959 1105 66-67 0.4865972288121547 1105 68-69 0.5232064427326151 1105 70-71 0.6664213268678338 1105 72-73 0.7044184717479247 1105 74-75 0.32426639849331096 1105 76-77 0.6230317425315413 1105 78-79 0.8065225319961833 1105 80-81 0.6250732972218636 1105 82-83 0.6594473190157721 1105 84-85 0.5929568782639763 1105 86-87 0.7956033966110887 1105 88-89 0.9561864938771727 1105 90-91 0.7242040088735102 1105 92-93 0.8736539314687555 1105 94-95 1.1042050738919826 1105 96-97 1.0848197063920288 1105 98-99 1.0190739790089616 1105 100-101 0.7527454631397923 1106 1 0.37611873426367026 1106 2 0.39133949999253304 1106 3 0.3066671803648404 1106 4 0.3872833300187466 1106 5 0.42266783365118954 1106 6 -0.06841990729004976 1106 7 0.27430465057545916 1106 8 0.32900993895592734 1106 9 0.3502627748391376 1106 10-11 0.23988288486328457 1106 12-13 0.23380577288580895 1106 14-15 0.5908773767991349 1106 16-17 0.4364369301710269 1106 18-19 0.0243471900400678 1106 20-21 0.37626369432768314 1106 22-23 0.39770276017694783 1106 24-25 0.6116680262392649 1106 26-27 0.3988184941772843 1106 28-29 0.45613158901341677 1106 30-31 0.3479212078408054 1106 32-33 0.5604959638614062 1106 34-35 0.3782800482481008 1106 36-37 0.24739099625598726 1106 38-39 0.33993171376780396 1106 40-41 0.5311297433785711 1106 42-43 0.14086690508582933 1106 44-45 0.4606049108902255 1106 46-47 0.2699297631774513 1106 48-49 0.5582984778905669 1106 50-51 0.3108906234518116 1106 52-53 0.5319666189116816 1106 54-55 0.3875104672122873 1106 56-57 -0.026761081442202794 1106 58-59 -0.40956518186916213 1106 60-61 0.4527107923361662 1106 62-63 0.42802038994462777 1106 64-65 0.5924699873998485 1106 66-67 0.015469075497858142 1106 68-69 0.07837915253175964 1106 70-71 -0.14100556543803222 1106 72-73 0.3913993469207462 1106 74-75 0.35383369029331035 1106 76-77 0.4616441129050166 1106 78-79 0.6449856600211952 1106 80-81 0.5272439859323192 1106 82-83 0.4689417399364224 1106 84-85 0.4812411058456938 1106 86-87 0.6760301039649015 1106 88-89 0.5009991771346023 1106 90-91 0.6384421558177458 1106 92-93 0.6617940049026849 1106 94-95 0.5321737476518997 1106 96-97 0.5991462218825951 1106 98-99 0.39344307385497856 1106 100-101 -0.06102350785873156 1107 1 0.42346288373789065 1107 2 0.5424506273460921 1107 3 0.33162565691779733 1107 4 0.364246261136671 1107 5 0.4641640376805398 1107 6 0.3492263450568913 1107 7 0.35962511811872844 1107 8 0.34998451236466366 1107 9 0.44227804687391625 1107 10-11 0.5344381138718219 1107 12-13 0.4074166394373506 1107 14-15 0.5613411879992896 1107 16-17 0.5081197222654126 1107 18-19 0.5422777442759212 1107 20-21 0.5943346608278546 1107 22-23 0.6386436275546359 1107 24-25 0.6504492867668574 1107 26-27 0.6919964005804786 1107 28-29 0.646629414112823 1107 30-31 0.8100962213903316 1107 32-33 0.5713391816467066 1107 34-35 0.7018859645447151 1107 36-37 0.7126052116162143 1107 38-39 0.8463205880754003 1107 40-41 0.7531456311497564 1107 42-43 0.743478546623578 1107 44-45 0.8251757924497838 1107 46-47 0.7462546429814907 1107 48-49 0.7478718678007752 1107 50-51 0.7568398752545562 1107 52-53 0.792895607892433 1107 54-55 0.7670007194183697 1107 56-57 0.7844013115043822 1107 58-59 0.710478832209489 1107 60-61 0.6460056222101933 1107 62-63 0.7492692872116642 1107 64-65 0.6150652986335601 1107 66-67 0.6604737256366562 1107 68-69 0.5768194899996075 1107 70-71 0.6830433266904841 1107 72-73 0.6198336222643377 1107 74-75 0.6637130037427887 1107 76-77 0.8690098375171296 1107 78-79 0.8421925202107126 1107 80-81 0.6574758904249585 1107 82-83 0.8470528976338265 1107 84-85 0.859079445802621 1107 86-87 0.8722702247732705 1107 88-89 1.0095591313663412 1107 90-91 1.0063516354783992 1107 92-93 1.0796364401711784 1107 94-95 0.799234241981921 1107 96-97 0.834986720903732 1107 98-99 0.7478224658219261 1107 100-101 0.6623151057465169 1108 1 0.30361699903562567 1108 2 0.2650413904638782 1108 3 0.20672367462136876 1108 4 0.24364982872593544 1108 5 0.2761159039716361 1108 6 0.3124580121317848 1108 7 0.2127348520208585 1108 8 0.2748310859549079 1108 9 0.24476620820637862 1108 10-11 0.39213490620526414 1108 12-13 0.41442479893277806 1108 14-15 0.4042211479927573 1108 16-17 0.38971922660625324 1108 18-19 0.4315164635226054 1108 20-21 0.4371481771502488 1108 22-23 0.4344134301074334 1108 24-25 0.3272277233823644 1108 26-27 0.3183267623402273 1108 28-29 0.18537872522484378 1108 30-31 0.3503687403730211 1108 32-33 0.3213629950584149 1108 34-35 0.40447122931583124 1108 36-37 0.39775214887127675 1108 38-39 0.4954679191022322 1108 40-41 0.4327085359777527 1108 42-43 0.504783963834555 1108 44-45 0.40557135873271477 1108 46-47 0.45989517783071676 1108 48-49 0.6137270924633 1108 50-51 0.6840068744585039 1108 52-53 0.5631522101608368 1108 54-55 0.516556131526464 1108 56-57 0.4946335518021172 1108 58-59 0.5735486318706648 1108 60-61 0.6342530656703467 1108 62-63 0.482637851637147 1108 64-65 0.4067300517224979 1108 66-67 0.44820299351032133 1108 68-69 0.3607284062512406 1108 70-71 0.5903453692008291 1108 72-73 0.3832621539633898 1108 74-75 0.4169029378136564 1108 76-77 0.4977885156686632 1108 78-79 0.46662971573923784 1108 80-81 0.4203916896030577 1108 82-83 0.6469627255989359 1108 84-85 0.7065367900288884 1108 86-87 0.5458091869167454 1108 88-89 0.6959585052437092 1108 90-91 0.6809176573118982 1108 92-93 0.7932318996339482 1108 94-95 0.6754156636085824 1108 96-97 0.6675867287281392 1108 98-99 0.5903442678508277 1108 100-101 0.44538789484133545 1109 1 0.5057726204436719 1109 2 0.6168158157512345 1109 3 0.541736695454702 1109 4 0.44702222455926943 1109 5 0.451294603684282 1109 6 0.47123584977546784 1109 7 0.5187284763024707 1109 8 0.6594036434261739 1109 9 0.5856067254477608 1109 10-11 0.5345823738776758 1109 12-13 0.4208404778120851 1109 14-15 0.49923731178586195 1109 16-17 0.6585909040487863 1109 18-19 0.6277916072007557 1109 20-21 0.5109572198945003 1109 22-23 0.688782144187897 1109 24-25 0.576440187462822 1109 26-27 0.5518904297252831 1109 28-29 0.5092108911731188 1109 30-31 0.38560715632129217 1109 32-33 0.5988270755181944 1109 34-35 0.6352172332669852 1109 36-37 0.6293995087850703 1109 38-39 0.5773117140016595 1109 40-41 0.550214821897292 1109 42-43 0.6502958172828315 1109 44-45 0.6940730649108673 1109 46-47 0.7552476383192257 1109 48-49 0.9117003324058359 1109 50-51 0.8697834100188544 1109 52-53 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-1.9852318632222747 2316 72-73 -1.7230633104708524 2316 74-75 -1.7544246231954475 2316 76-77 -1.5479266247895929 2316 78-79 -1.5895836248524127 2316 80-81 -1.6783373691697 2316 82-83 -1.3159398535559639 2316 84-85 -1.3583960337509282 2316 86-87 -1.3739680121573272 2316 88-89 -1.661760997561533 2316 90-91 -1.6479457416405516 2316 92-93 -1.787014809601768 2316 94-95 -2.000387962117248 2316 96-97 -2.194151515739847 2316 98-99 -2.242819561696411 2316 100-101 -1.9502915670623064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9797.0 3 3094.0 4 3423.0 5 3796.0 6 4407.0 7 5659.0 8 7195.0 9 9123.0 10 11628.0 11 14832.0 12 18435.0 13 22463.0 14 26099.0 15 31558.0 16 36638.0 17 42398.0 18 39380.0 19 33991.0 20 35156.0 21 38345.0 22 42297.0 23 47209.0 24 53020.0 25 59676.0 26 67206.0 27 76463.0 28 87583.0 29 100562.0 30 115860.0 31 136203.0 32 160506.0 33 192873.0 34 235632.0 35 290250.0 36 342002.0 37 343523.0 38 162838.0 39 8556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42259135118219 19.064060436860554 15.171907126641246 22.341441085316006 2 15.406045457956582 20.610247829440656 40.277981000302574 23.705725712300186 3 18.873333452123628 25.26683269894747 29.812206251019425 26.04762759790947 4 12.736224877413393 17.132174216393246 37.222944333395816 32.90865657279755 5 13.820370042893392 37.73367576763269 33.405404923761076 15.04054926571284 6 31.244409288412516 36.38741840559931 17.813335179481975 14.554837126506193 7 28.43376895524066 33.23623151759105 20.42701499972932 17.902984527438974 8 26.680298619250863 34.12498248323787 20.025265252602846 19.169453644908423 9 27.241671149609214 16.66465648096434 20.03399041278478 36.05968195664167 10-11 24.908430870296925 26.957293895145785 27.073640957181162 21.060634277376124 12-13 26.14034351344475 24.39219569524596 27.531614938063587 21.9358458532457 14-15 23.022800523938887 25.617791857293465 26.47722705720191 24.882180561565733 16-17 21.994691170537795 28.813496315596975 26.878043390558716 22.313769123306514 18-19 22.000498548470134 26.90239500286537 29.857074205770314 21.240032242894184 20-21 23.046950630064565 26.04839707312025 29.78391079685271 21.120741499962474 22-23 22.554614511601386 26.01251699635949 29.6505945984473 21.782273893591825 24-25 22.087855894069513 26.306745720154357 29.853013705719633 21.752384680056498 26-27 21.8163057630858 26.64284535687501 29.695448997820684 21.84539988221851 28-29 21.77485123422651 26.708947465447896 29.88533573469356 21.63086556563204 30-31 21.896489360407926 26.399971351089146 29.731332501623925 21.972206786879003 32-33 21.819330411321108 26.509387910915468 29.424015431459416 22.247266246304008 34-35 22.411796443433026 26.620504068297436 29.28024486029315 21.68745462797639 36-37 22.46069492324881 26.185919956303227 29.342713472788123 22.01067164765984 38-39 22.003916888511196 26.563410820896994 29.200432971547315 22.232239319044496 40-41 22.184963713779513 26.413836777699345 29.37267927749936 22.028520231021787 42-43 22.256179445788877 26.311393640083423 29.027824977595312 22.404601936532384 44-45 22.06443175909745 26.7122135778273 28.9923049890497 22.231049674025552 46-47 22.30140947641413 26.88857062505502 28.436966804838537 22.373053093692313 48-49 22.305854167672887 26.880108819153925 28.339565908584103 22.474471104589085 50-51 22.452497218614184 27.118015022223684 28.018385919337067 22.411101839825058 52-53 22.603751500954907 27.077526258468588 27.894477325154917 22.42424491542159 54-55 22.209059080677974 27.13051925653237 28.12452579292561 22.53589586986405 56-57 22.339920658884413 27.301506343983796 27.925140597300253 22.433432399831542 58-59 22.28823744104231 26.813886123347903 28.113194653418105 22.784681782191683 60-61 22.25837948951229 26.957532090668952 28.240696416371406 22.543392003447348 62-63 22.16304280253333 26.987213991430554 28.237899668734162 22.611843537301958 64-65 21.963649529177758 26.83087358069127 28.22900549997654 22.976471390154433 66-67 22.06525959174265 27.00727901901505 28.08999520646208 22.837466182780222 68-69 22.247803840085716 26.783029059941484 28.13549225974802 22.833674840224784 70-71 22.174355868279864 26.84392329309248 28.013293561817427 22.968427276810228 72-73 22.324084587595305 26.83036661715907 27.83208616548944 23.013462629756184 74-75 22.205710133711946 27.057780490967424 27.71394759330315 23.02256178201748 76-77 22.277206779902542 26.809484930400373 27.838788314134412 23.074519975562673 78-79 22.38948653054206 26.929907577621197 27.704295655706346 22.97631023613039 80-81 22.314518084752194 27.13507492440424 27.588889239027825 22.961517751815737 82-83 22.505768516411052 27.0681242392777 27.42869825996575 22.997408984345498 84-85 22.492907241481237 26.866526666819656 27.498238584537837 23.142327507161262 86-87 22.611941512705787 26.82437969643125 27.393581811564726 23.170096979298236 88-89 22.554181038285392 26.70682583011355 27.39952429121189 23.339468840389166 90-91 22.40564352868559 26.82665093309308 27.509409907046173 23.25829563117516 92-93 22.619466874042914 26.448950157758965 27.546668219704628 23.384914748493497 94-95 22.421236975756145 26.466733327862933 27.572673066575486 23.539356629805436 96-97 22.678270772988075 26.276646992998753 27.457718060098223 23.587364173914953 98-99 22.63530358893985 26.251838148704838 27.203590224517498 23.909268037837812 100-101 23.23054089653212 25.7126891608424 27.368974964190222 23.68779497843526 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1424.0 1 944.5 2 601.5 3 750.0 4 663.5 5 669.0 6 1129.5 7 2485.0 8 3992.5 9 4356.5 10 3710.5 11 3074.5 12 3061.5 13 3372.0 14 3663.0 15 3923.0 16 4194.5 17 4544.5 18 4901.5 19 5207.5 20 5737.5 21 6496.5 22 7612.5 23 9145.0 24 10808.0 25 12996.0 26 16308.0 27 20534.5 28 24570.5 29 28754.0 30 33575.0 31 39217.5 32 46162.0 33 51278.0 34 54905.5 35 59343.5 36 65261.5 37 71365.0 38 75372.5 39 78556.0 40 82532.5 41 88778.5 42 95094.0 43 99382.0 44 102176.0 45 106157.0 46 108145.5 47 108569.5 48 111300.5 49 111779.0 50 109247.5 51 104828.0 52 100965.5 53 99403.0 54 97583.5 55 91175.0 56 81764.0 57 74058.5 58 68069.0 59 59756.5 60 49749.5 61 41481.5 62 34120.5 63 26396.5 64 19818.0 65 15688.0 66 12486.0 67 9912.0 68 7638.0 69 5720.5 70 4557.0 71 3751.0 72 3166.0 73 2688.0 74 2186.0 75 1804.5 76 1286.5 77 842.5 78 600.0 79 389.0 80 240.0 81 158.0 82 99.5 83 62.5 84 39.0 85 21.5 86 13.0 87 10.5 88 8.0 89 5.0 90 5.0 91 4.0 92 3.0 93 2.0 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1859110394441027 2 0.04860128315607622 3 0.046854513994018514 4 0.052574326740364344 5 0.059732655267228284 6 0.06473321012331505 7 0.03733290954201767 8 0.03572314188286646 9 0.0413059531263058 10-11 0.0428985955975937 12-13 0.039679060279291266 14-15 0.034764131362521045 16-17 0.03265773325533381 18-19 0.041220327186989245 20-21 0.05079330720258002 22-23 0.04808752752017689 24-25 0.06812399732025061 26-27 0.05344771132139319 28-29 0.04555299971640689 30-31 0.054440972217465224 32-33 0.05074193163899008 34-35 0.049337666234198586 36-37 0.04735114444205453 38-39 0.026629667127448388 40-41 0.025019899468297167 42-43 0.02205724196794439 44-45 0.0041785458386478505 46-47 0.012501387140216928 48-49 0.01154237661987152 50-51 0.009179100694734621 52-53 0.00974423189422388 54-55 0.011268373614058547 56-57 0.008785221373878471 58-59 0.010805993541749152 60-61 0.01131974917764848 62-63 0.004024419147878052 64-65 0.00715832852686394 66-67 0.004127170275057918 68-69 0.008973598440374892 70-71 0.02291350136110993 72-73 0.020396098745203234 74-75 0.020635851375289588 76-77 0.012724014582439968 78-79 0.00496630448036015 80-81 0.006850075145324344 82-83 0.0056341868070292735 84-85 0.005103305983266636 86-87 0.00816871461079928 88-89 0.02991770319720407 90-91 0.03687052946970828 92-93 0.022810750233930064 94-95 0.03207547686798124 96-97 0.03609989601585929 98-99 0.02186886490144797 100-101 0.12237659247121942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2919676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.64603960700369 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6399306353742 33.65712932889032 2 14.758171647678871 14.063368617045327 3 5.773472547325109 8.252493049794019 4 2.9617628860149194 5.644650870944816 5 1.6857029497274685 4.015853475417896 6 1.0120275152123437 2.893146184391091 7 0.6500854116318473 2.1681796689382407 8 0.47343814423541725 1.8045962057365612 9 0.3370699389577813 1.4454042895721488 >10 1.5270852594706241 12.655301955170367 >50 0.10539534508712889 3.507912834089779 >100 0.06971814465268342 6.203033339194738 >500 0.004189356807466291 1.3599381008460696 >1k 0.0017335269546986999 1.2122294681090473 >5k 7.22302897791125E-5 0.2713956843489651 >10k+ 1.44460579558225E-4 0.8453669275105223 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14220 0.4870403428325609 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10344 0.3542858865161751 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7886 0.2700984629801389 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.425037572662172E-5 0.0 0.0 0.0 0.0 3 3.425037572662172E-5 0.0 0.0 0.0 0.0 4 3.425037572662172E-5 0.0 0.0 0.0 0.0 5 6.850075145324345E-5 0.0 0.0 0.0 0.0 6 1.0275112717986517E-4 0.0 0.0 0.0 0.0 7 2.0550225435973034E-4 0.0 0.0 0.0 0.0 8 2.3975263008635205E-4 0.0 0.0 0.0 0.0 9 2.3975263008635205E-4 0.0 0.0 0.0 0.0 10-11 2.3975263008635205E-4 0.0 0.0 0.0 0.0 12-13 3.425037572662172E-4 0.0 0.0 0.0 0.0 14-15 4.966304480360149E-4 0.0 0.0 0.0 0.0 16-17 5.308808237626367E-4 0.0 0.0 0.0 3.425037572662172E-5 18-19 6.336319509425018E-4 0.0 0.0 1.712518786331086E-5 3.425037572662172E-5 20-21 7.192578902590561E-4 0.0 0.0 2.226274422230412E-4 3.425037572662172E-5 22-23 7.706334538489887E-4 0.0 0.0 6.165067630791909E-4 3.425037572662172E-5 24-25 0.0010275112717986516 0.0 0.0 0.001472766156244734 3.425037572662172E-5 26-27 0.0010617616475252735 0.0 0.0 0.0026030285552232505 3.425037572662172E-5 28-29 0.001250138714021693 0.0 0.0 0.0062164431943818425 3.425037572662172E-5 30-31 0.0013015142776116255 0.0 0.0 0.018238325074426066 3.425037572662172E-5 32-33 0.001352889841201558 0.0 0.0 0.04157995613211877 3.425037572662172E-5 34-35 0.00159264247128791 0.0 0.0 0.07372393375155326 3.425037572662172E-5 36-37 0.0018837706649641947 0.0 0.0 0.1264695123705507 3.425037572662172E-5 38-39 0.002003646980007371 0.0 0.0 0.20666676713443546 3.425037572662172E-5 40-41 0.002072147731460614 0.0 0.0 0.2973960124342564 3.425037572662172E-5 42-43 0.0022776499858203443 0.0 0.0 0.3887588896850198 3.425037572662172E-5 44-45 0.0025687781794966292 0.0 0.0 0.5015625021406485 3.425037572662172E-5 46-47 0.0030140330639427115 0.0 0.0 0.6346252118385739 3.425037572662172E-5 48-49 0.0031167841911225766 0.0 0.0 0.7779801594423491 3.425037572662172E-5 50-51 0.0031167841911225766 0.0 0.0 0.9353606359061759 3.425037572662172E-5 52-53 0.0031167841911225766 0.0 0.0 1.0917821018496574 5.1375563589932585E-5 54-55 0.0031510345668491983 0.0 0.0 1.2615440891386578 6.850075145324345E-5 56-57 0.0032537856940290633 0.0 0.0 1.4557779698843296 6.850075145324345E-5 58-59 0.0032537856940290633 0.0 0.0 1.6658868997792906 6.850075145324345E-5 60-61 0.003322286445482307 0.0 0.0 1.9011013550818654 6.850075145324345E-5 62-63 0.0033907871969355505 0.0 0.0 2.1335929055141736 6.850075145324345E-5 64-65 0.003425037572662172 0.0 0.0 2.3725749021466767 6.850075145324345E-5 66-67 0.0035962894512952806 0.0 0.0 2.647639669607176 6.850075145324345E-5 68-69 0.0037332909542017677 0.0 0.0 2.9557389244560013 6.850075145324345E-5 70-71 0.0038874176449715655 0.0 0.0 3.2855186671397787 6.850075145324345E-5 72-73 0.00397304358428812 0.0 0.0 3.6306083277733556 6.850075145324345E-5 74-75 0.004041544335741363 0.0 0.0 3.9881137496078334 6.850075145324345E-5 76-77 0.004075794711467985 0.0 0.0 4.371255577673687 6.850075145324345E-5 78-79 0.004092919899331296 0.0 0.0 4.783082095410586 8.562593931655431E-5 80-81 0.0047608022260004195 0.0 0.0 5.223884431012208 1.0275112717986517E-4 82-83 0.004897803728906906 0.0 0.0 5.683490222887745 1.0275112717986517E-4 84-85 0.005017680043950082 0.0 0.0 6.142085628679347 1.0275112717986517E-4 86-87 0.00517180673471988 0.0 0.0 6.62256702456026 1.0275112717986517E-4 88-89 0.005206057110446502 0.0 0.0 7.140929335994816 1.0275112717986517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6525 0.0 54.199593 1 GTATCAA 12570 0.0 45.098717 1 TATCAAC 17035 0.0 33.145813 2 ATCAACG 17130 0.0 32.93481 3 TCAACGC 17475 0.0 32.311794 4 CAACGCA 17870 0.0 31.595406 5 AACGCAG 18475 0.0 30.553942 6 GTACATG 16200 0.0 26.854612 1 ACGCAGA 21105 0.0 26.653666 7 CGCAGAG 21195 0.0 26.40599 8 TACATGG 16360 0.0 26.175552 2 TGGTATC 3110 0.0 25.8274 2 GTGGTAT 3355 0.0 24.54377 1 GCAGAGT 22280 0.0 24.3503 9 ACATGGG 16935 0.0 24.27687 3 AGTACTT 12500 0.0 21.982635 12-13 GAGTACT 11910 0.0 21.895102 12-13 CATGGGA 9895 0.0 21.711231 4 AGAGTAC 21030 0.0 21.300625 10-11 CATGGGG 6675 0.0 20.720655 4 >>END_MODULE