FastQCFastQC Report
Thu 26 May 2016
SRR938674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919676
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139480.47772424063491975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108530.3717193277610255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82330.2819833433572766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69150.057.1602941
GTATCAA130500.044.721841
TATCAAC175650.033.1986542
ATCAACG178450.032.6076623
TCAACGC181350.032.085684
CAACGCA184900.031.4696465
AACGCAG191850.030.3753196
ACGCAGA219850.026.485137
CGCAGAG221750.026.1296928
GCAGAGT236650.023.90259
GTACATG158450.023.5347421
TACATGG160150.023.0469252
GAGTACT129600.022.46433412-13
TGGTATC30850.022.0474852
AGTACTT135350.022.00125112-13
ACATGGG164150.021.6049233
GTGGTAT33450.021.3295061
GTATAGG6500.021.2212161
AGAGTAC221750.020.97871610-11
GTACTTT138450.020.7710914-15
Produced by FastQC (version 0.11.5)