##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938674_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2919676 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79197178043043 31.0 31.0 34.0 30.0 34.0 2 32.013539516028494 31.0 31.0 34.0 30.0 34.0 3 32.07099143877608 33.0 31.0 34.0 30.0 34.0 4 35.75083125661889 37.0 35.0 37.0 33.0 37.0 5 35.6210391152991 37.0 35.0 37.0 33.0 37.0 6 35.65320535566275 37.0 35.0 37.0 33.0 37.0 7 35.594134075150805 37.0 35.0 37.0 33.0 37.0 8 35.58841939996082 37.0 35.0 37.0 33.0 37.0 9 37.15982526828319 39.0 37.0 39.0 33.0 39.0 10-11 37.07429728504122 39.0 37.0 39.0 33.0 39.0 12-13 36.96868197019121 39.0 37.0 39.0 33.0 39.0 14-15 37.99421562529541 40.0 37.0 41.0 33.0 41.0 16-17 37.93319447089334 40.0 37.0 41.0 33.0 41.0 18-19 38.06863004662161 40.0 37.0 41.0 33.0 41.0 20-21 38.13157384586509 40.0 37.0 41.0 33.0 41.0 22-23 38.025177622448524 40.0 37.0 41.0 33.0 41.0 24-25 37.928043899391575 40.0 37.0 41.0 33.0 41.0 26-27 37.73782142265101 40.0 37.0 41.0 32.0 41.0 28-29 37.604663154404804 39.5 37.0 41.0 32.0 41.0 30-31 37.3528485694988 39.0 36.0 41.0 31.5 41.0 32-33 37.11917657986708 39.0 36.0 41.0 31.0 41.0 34-35 36.94655708373121 39.0 35.5 41.0 31.0 41.0 36-37 36.925700488684356 39.0 35.5 41.0 31.0 41.0 38-39 36.7894072835479 39.0 35.0 41.0 30.0 41.0 40-41 36.60227093691218 39.0 35.0 41.0 30.0 41.0 42-43 36.360656627653206 39.0 35.0 40.5 30.0 41.0 44-45 36.045603347768726 39.0 35.0 40.0 29.5 41.0 46-47 35.62285250144194 38.0 35.0 40.0 27.5 41.0 48-49 35.224902180926925 38.0 34.0 40.0 26.5 41.0 50-51 34.85469997355871 38.0 34.0 40.0 26.0 41.0 52-53 34.38139540140755 38.0 33.0 40.0 24.0 41.0 54-55 34.07217684427998 38.0 33.0 40.0 23.5 41.0 56-57 33.753483605715154 37.0 33.0 40.0 23.0 41.0 58-59 33.33023938272603 37.0 32.0 40.0 22.0 41.0 60-61 32.971680076830445 36.0 32.0 39.0 20.5 40.5 62-63 32.53069227544426 36.0 31.0 39.0 19.5 40.0 64-65 32.05320419114998 35.0 31.0 39.0 18.0 40.0 66-67 31.579702850590273 35.0 30.0 38.0 15.5 40.0 68-69 31.059109127177123 35.0 29.5 38.0 12.0 40.0 70-71 30.50911573749964 34.0 29.0 37.0 8.0 39.0 72-73 30.077018991148332 34.0 29.0 36.5 7.5 39.0 74-75 29.583418331349094 34.0 28.5 36.0 7.0 39.0 76-77 28.30202323819492 32.0 26.5 34.5 2.0 37.0 78-79 29.007026293328437 33.5 28.0 35.0 2.0 37.0 80-81 28.750027057796824 33.5 28.0 35.0 2.0 37.0 82-83 28.291775868281277 33.0 27.0 35.0 2.0 36.5 84-85 27.785810994096607 33.0 26.0 35.0 2.0 36.0 86-87 27.232562448710063 32.5 26.0 35.0 2.0 35.5 88-89 26.614822843356592 32.0 25.0 34.5 2.0 35.0 90-91 26.10190480039566 32.0 24.0 34.0 2.0 35.0 92-93 25.395634652612138 31.0 21.5 34.0 2.0 35.0 94-95 24.648431367042097 31.0 18.5 34.0 2.0 35.0 96-97 23.67616886257242 31.0 4.5 34.0 2.0 35.0 98-99 22.471883695314137 30.5 2.0 34.0 2.0 35.0 100-101 18.826279868040153 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3224548254506914 1101 2 0.30357333002207554 1101 3 0.2802956047799725 1101 4 0.18174496267905482 1101 5 0.039890158289438205 1101 6 0.16055666672317415 1101 7 0.19699245283275957 1101 8 0.2502741395899051 1101 9 0.33782050478497894 1101 10-11 0.38462737584964657 1101 12-13 0.32547269594675754 1101 14-15 0.4610016203853675 1101 16-17 0.43821265937801 1101 18-19 0.4745931550884208 1101 20-21 0.39517193840949005 1101 22-23 0.45539648372176345 1101 24-25 0.5257872934793966 1101 26-27 0.483858790463195 1101 28-29 0.43320664882172366 1101 30-31 0.4563976329973016 1101 32-33 0.3817143324956831 1101 34-35 0.28613950512290387 1101 36-37 0.43750594739452 1101 38-39 0.573503723264416 1101 40-41 0.5058191460261554 1101 42-43 0.5310640051463409 1101 44-45 0.5228315463912807 1101 46-47 0.5597979633781378 1101 48-49 0.6737081586351223 1101 50-51 0.6607501582456408 1101 52-53 0.7458220812296048 1101 54-55 0.5830360651466648 1101 56-57 0.7576498973271342 1101 58-59 0.6376330980777638 1101 60-61 0.6997921927539821 1101 62-63 0.7237117038252023 1101 64-65 0.6045633325995183 1101 66-67 0.4301965449834455 1101 68-69 0.4891811956930887 1101 70-71 0.3519965779762444 1101 72-73 0.2381748954558489 1101 74-75 0.43964213218347936 1101 76-77 0.5956911319850065 1101 78-79 0.6104341520501784 1101 80-81 0.5826053259359263 1101 82-83 0.6371912727145457 1101 84-85 0.6563779907515617 1101 86-87 0.7088925237865595 1101 88-89 0.7038102865448508 1101 90-91 0.7644125376189734 1101 92-93 0.7705347789225563 1101 94-95 0.9555272938024899 1101 96-97 1.122364881599779 1101 98-99 0.8613158894469706 1101 100-101 0.8188217609779365 1102 1 0.32400620943607805 1102 2 0.32132845919594644 1102 3 0.29716397081434565 1102 4 0.21963001800416038 1102 5 0.2146694642969038 1102 6 0.2312409830743647 1102 7 0.29837463913248996 1102 8 0.327593133561777 1102 9 0.34175946691784276 1102 10-11 0.39813880923147593 1102 12-13 0.401062133643336 1102 14-15 0.5100402610579096 1102 16-17 0.473623863657366 1102 18-19 0.4505899092269132 1102 20-21 0.3829136848646897 1102 22-23 0.38911937878460634 1102 24-25 0.4800897479225128 1102 26-27 0.37273860286896365 1102 28-29 0.3886385002478434 1102 30-31 0.3299103111539594 1102 32-33 0.34549207961718054 1102 34-35 0.29814908155353237 1102 36-37 0.31025891002113326 1102 38-39 0.28719759395703903 1102 40-41 0.29561328444260937 1102 42-43 0.3123719328477037 1102 44-45 0.42552120471587074 1102 46-47 0.1952869799768493 1102 48-49 0.25909922562526333 1102 50-51 0.3112045357894573 1102 52-53 0.27767720277645225 1102 54-55 0.33815370817664103 1102 56-57 0.29822913995667477 1102 58-59 0.2958530089457767 1102 60-61 0.378858608854145 1102 62-63 0.45829987021214436 1102 64-65 0.3942348690309174 1102 66-67 0.38489403483109186 1102 68-69 0.43053319898173825 1102 70-71 0.4310645778300959 1102 72-73 0.22894286178691559 1102 74-75 0.30360234440091816 1102 76-77 0.3627143655126872 1102 78-79 0.43542207592830806 1102 80-81 0.4205467573352877 1102 82-83 0.42857666681011963 1102 84-85 0.32638936460370616 1102 86-87 0.22430098844988677 1102 88-89 0.3552477722441836 1102 90-91 0.17886253955698805 1102 92-93 0.006167667040500646 1102 94-95 0.07268304166834483 1102 96-97 0.2681664269821695 1102 98-99 0.13091529284417547 1102 100-101 0.12891211968286598 1103 1 0.32214648556205105 1103 2 0.25959264190687037 1103 3 0.2582791962611921 1103 4 0.15953777305271188 1103 5 0.16951947194096562 1103 6 0.23078816347485542 1103 7 0.26801891565026636 1103 8 0.2705881626059039 1103 9 0.3377615238651046 1103 10-11 0.3352072093461089 1103 12-13 0.3788895923008866 1103 14-15 0.5083968872673097 1103 16-17 0.524818885156698 1103 18-19 0.4750022596465371 1103 20-21 0.494727431864149 1103 22-23 0.52343399773644 1103 24-25 0.5218096417395586 1103 26-27 0.5683378740689875 1103 28-29 0.5206866386377698 1103 30-31 0.5406358218665659 1103 32-33 0.5400331613146463 1103 34-35 0.44160434261971915 1103 36-37 0.39601011602890424 1103 38-39 0.4854457473041762 1103 40-41 0.5301607328816047 1103 42-43 0.45370750579157004 1103 44-45 0.5263562273535456 1103 46-47 0.5908707645083169 1103 48-49 0.5769030704223752 1103 50-51 0.5046277976921871 1103 52-53 0.6934229752137568 1103 54-55 0.6909694691048358 1103 56-57 0.5777439174814205 1103 58-59 0.5802827520854734 1103 60-61 0.6254288743181462 1103 62-63 0.594499891787585 1103 64-65 0.502663918579664 1103 66-67 0.6311110851237203 1103 68-69 0.6860383456517418 1103 70-71 0.5301463639856756 1103 72-73 0.5097137802059244 1103 74-75 0.5645754763546122 1103 76-77 0.3962709318932447 1103 78-79 0.5655897091881137 1103 80-81 0.5864170518533385 1103 82-83 0.7258598153954345 1103 84-85 0.5829556127994096 1103 86-87 0.7408395591892187 1103 88-89 0.8402171662190732 1103 90-91 0.7717658729631225 1103 92-93 0.6144245101294423 1103 94-95 0.6761954710435134 1103 96-97 0.7682629870224602 1103 98-99 0.6631941428862369 1103 100-101 0.22478776835256653 1104 1 0.1859246957563414 1104 2 0.16709484689886978 1104 3 0.14789131699011193 1104 4 0.13423704702483974 1104 5 0.13465468653527068 1104 6 0.07660366170338762 1104 7 0.10158449983610751 1104 8 0.1880578034787277 1104 9 0.2280209405512892 1104 10-11 0.23397864261582413 1104 12-13 0.13809468645528966 1104 14-15 0.33095071647884566 1104 16-17 0.2937640643216426 1104 18-19 0.22652924775204752 1104 20-21 0.21675081723695655 1104 22-23 0.2894300312423539 1104 24-25 0.29623665525853227 1104 26-27 0.3136307767354083 1104 28-29 0.20904882788339307 1104 30-31 0.22489429675955108 1104 32-33 0.22076439645898205 1104 34-35 0.14413510038951216 1104 36-37 0.31730410997949576 1104 38-39 0.2267848626165403 1104 40-41 0.29168729742931987 1104 42-43 0.36837201065252145 1104 44-45 0.30645150294017043 1104 46-47 0.2678011291168261 1104 48-49 0.14160700353014022 1104 50-51 0.24626321659084027 1104 52-53 0.4100614462199772 1104 54-55 0.4050620463792143 1104 56-57 0.33586464141641414 1104 58-59 0.3700469176018899 1104 60-61 0.17743367297870805 1104 62-63 0.13471178279868923 1104 64-65 0.22163133452835382 1104 66-67 0.09621229051934677 1104 68-69 0.09676008811863923 1104 70-71 -0.01932873579955796 1104 72-73 -0.1672605596668113 1104 74-75 -0.09032869992515558 1104 76-77 0.05371371421942328 1104 78-79 -0.08613224007464737 1104 80-81 0.10371203742746005 1104 82-83 -0.026131529076636184 1104 84-85 -0.05033322620378655 1104 86-87 0.07135907772859085 1104 88-89 0.0076022823984800425 1104 90-91 -0.11833470955341241 1104 92-93 -0.07829982600601681 1104 94-95 -0.0834529884519064 1104 96-97 -0.10744248584243365 1104 98-99 -0.13561584659956338 1104 100-101 -0.11742113533039245 1105 1 0.23835628761342775 1105 2 0.24955685764476243 1105 3 0.21993726330236285 1105 4 0.1502998457521656 1105 5 0.13198148273772148 1105 6 0.19890711890796098 1105 7 0.2066721881237754 1105 8 0.18574653185638823 1105 9 0.26213639490115526 1105 10-11 0.2696384988760272 1105 12-13 0.237329084394446 1105 14-15 0.38912512347874184 1105 16-17 0.28375323360496196 1105 18-19 0.3301809953098527 1105 20-21 0.3031933710777679 1105 22-23 0.30685466641946135 1105 24-25 0.32459940204770277 1105 26-27 0.3650873434527213 1105 28-29 0.2664570167676814 1105 30-31 0.37559562772218413 1105 32-33 0.3491282924272312 1105 34-35 0.426216744706025 1105 36-37 0.4990587476871653 1105 38-39 0.42236057053455056 1105 40-41 0.3257114690489118 1105 42-43 0.4207338816961439 1105 44-45 0.3590493111120878 1105 46-47 0.16540020967877922 1105 48-49 0.1515418344136279 1105 50-51 0.1372317707861228 1105 52-53 0.2411675441196124 1105 54-55 0.4417542090252553 1105 56-57 0.3287391387394223 1105 58-59 0.38758617450715604 1105 60-61 0.4081671525223669 1105 62-63 0.39818721074350094 1105 64-65 0.2607820888163346 1105 66-67 0.41047094916908833 1105 68-69 0.3007578570526057 1105 70-71 0.45972830471125903 1105 72-73 0.3818346809783968 1105 74-75 0.39582203351173817 1105 76-77 0.42116585861128897 1105 78-79 0.4034427508503917 1105 80-81 0.36980990388763857 1105 82-83 0.49297268464318833 1105 84-85 0.4424382596539651 1105 86-87 0.29886484494898014 1105 88-89 0.1773608529050037 1105 90-91 0.2487210272907383 1105 92-93 0.2634807831489354 1105 94-95 0.36052447199974935 1105 96-97 0.4420487476982444 1105 98-99 0.406762501539621 1105 100-101 0.126750394219858 1106 1 0.1863519922951795 1106 2 0.2109974114809816 1106 3 0.22604183494105 1106 4 0.17163429041313094 1106 5 0.04735489840067686 1106 6 0.08136945266600293 1106 7 0.1259370965805786 1106 8 0.13899969776138477 1106 9 0.14266152028606882 1106 10-11 0.11904685420919492 1106 12-13 0.19556441794446044 1106 14-15 0.28281167024155707 1106 16-17 0.050021024567833194 1106 18-19 0.29672861423385655 1106 20-21 0.25101848835156915 1106 22-23 0.3263777808690165 1106 24-25 0.24724496410407681 1106 26-27 0.15750790896756683 1106 28-29 0.3204331340690487 1106 30-31 0.36850312596467916 1106 32-33 0.35628281023599584 1106 34-35 0.29031125093769106 1106 36-37 -0.10588452579808916 1106 38-39 0.1569451974073317 1106 40-41 0.4252806334452117 1106 42-43 0.4804395898611773 1106 44-45 0.4559483816328225 1106 46-47 0.2442407252785017 1106 48-49 0.322803169361336 1106 50-51 0.38982747424486064 1106 52-53 0.318676070317089 1106 54-55 0.12086019947582116 1106 56-57 0.29312046488143295 1106 58-59 0.2977820286966306 1106 60-61 -0.006915797263317813 1106 62-63 0.040745094218166855 1106 64-65 -0.23292481074916438 1106 66-67 0.14689315490753785 1106 68-69 -0.5907722041177159 1106 70-71 -0.2985023213877973 1106 72-73 -0.19240239755907496 1106 74-75 0.0012422825428153317 1106 76-77 0.08679359777373818 1106 78-79 0.03066570899642329 1106 80-81 -0.6286245225319611 1106 82-83 -0.27886662836434084 1106 84-85 -0.06865886371274499 1106 86-87 -0.005683291102354104 1106 88-89 0.08401510020840064 1106 90-91 -0.1508308533763021 1106 92-93 -0.012061955371432731 1106 94-95 0.058894436339652856 1106 96-97 -0.03931661153161059 1106 98-99 -0.2657189054713207 1106 100-101 -0.47804300233407915 1107 1 0.18269456233907277 1107 2 0.19490133090899064 1107 3 0.1680526439943293 1107 4 0.034773363340022456 1107 5 0.14797276109101887 1107 6 0.13293438661055745 1107 7 0.1271556123501867 1107 8 0.11761005599815633 1107 9 0.184972356870297 1107 10-11 0.2532389072670682 1107 12-13 0.23892372295081543 1107 14-15 0.2619448507039337 1107 16-17 0.24178775393773577 1107 18-19 0.22304246393969152 1107 20-21 0.26819210763466117 1107 22-23 0.27659864861880834 1107 24-25 0.2962831639034462 1107 26-27 0.2949806964084942 1107 28-29 0.37797220619858507 1107 30-31 0.32376212396663817 1107 32-33 0.2972712310692387 1107 34-35 0.3300184416979164 1107 36-37 0.34815219113969675 1107 38-39 0.1693618099755767 1107 40-41 0.28431448322191955 1107 42-43 0.28732017841659285 1107 44-45 0.3161985322999712 1107 46-47 0.18307113397464292 1107 48-49 0.26957286777675193 1107 50-51 0.2609509650538868 1107 52-53 0.4170145454923997 1107 54-55 0.2331023017150855 1107 56-57 0.18298947471306093 1107 58-59 0.21753756583915873 1107 60-61 0.13998318364863138 1107 62-63 0.28648222704380544 1107 64-65 0.44470362510981687 1107 66-67 0.42154924508324143 1107 68-69 0.5000712307257409 1107 70-71 0.2543814623522884 1107 72-73 0.40401649722065613 1107 74-75 0.39960291758410804 1107 76-77 0.2593321859521751 1107 78-79 0.3856015339802177 1107 80-81 0.4860697277967816 1107 82-83 0.3411778286901459 1107 84-85 0.3240441947474402 1107 86-87 0.29232233203285674 1107 88-89 0.33630844146214045 1107 90-91 0.39830446887421544 1107 92-93 0.22722046586359212 1107 94-95 0.09591448033158301 1107 96-97 0.15954670140933302 1107 98-99 0.11854684769058466 1107 100-101 -0.3408980170087581 1108 1 0.18471147326330595 1108 2 0.1287668089462244 1108 3 0.1558525228861214 1108 4 0.04293434710608324 1108 5 0.07807572007752128 1108 6 0.035942573321307236 1108 7 0.0899509837033321 1108 8 0.055704115572510204 1108 9 0.05132660008006695 1108 10-11 0.13949191371030167 1108 12-13 0.08375446912705797 1108 14-15 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-0.8458292509084657 2316 58-59 -0.9658698587892474 2316 60-61 -0.718093546559416 2316 62-63 -0.8461759406501699 2316 64-65 -0.8537843507381417 2316 66-67 -0.7586080236336237 2316 68-69 -0.9447905154096823 2316 70-71 -1.0153333005125127 2316 72-73 -1.0650112298166832 2316 74-75 -0.6469769101485028 2316 76-77 -0.5458227763809909 2316 78-79 -0.6292601875537827 2316 80-81 -0.9050372871768779 2316 82-83 -0.6932819014404075 2316 84-85 -0.6723821405872208 2316 86-87 -0.5136359693698722 2316 88-89 -0.4483242161930221 2316 90-91 -0.41883354061619116 2316 92-93 -0.4792266043730713 2316 94-95 -0.5045170491841837 2316 96-97 -0.44172312170231365 2316 98-99 -0.3606409608266752 2316 100-101 -0.34370862743984887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 15.0 4 50.0 5 108.0 6 252.0 7 634.0 8 1415.0 9 2478.0 10 4132.0 11 6617.0 12 8945.0 13 12916.0 14 16795.0 15 22067.0 16 28141.0 17 35438.0 18 32512.0 19 25176.0 20 23869.0 21 24714.0 22 26767.0 23 30552.0 24 35721.0 25 41692.0 26 49803.0 27 60298.0 28 72631.0 29 87423.0 30 104129.0 31 123427.0 32 149391.0 33 185152.0 34 236888.0 35 311422.0 36 413120.0 37 484148.0 38 250165.0 39 10668.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.138163584396096 19.821040499648074 15.790804050478565 22.249991865477256 2 15.784544625592009 21.31008787124599 39.351055616608186 23.554311886553812 3 18.758845232240006 25.468343487511525 30.32481268315539 25.44799859709308 4 13.156185823358483 17.92215985609362 36.81507811140688 32.10657620914101 5 13.716800659813266 37.831762014734046 33.61273472510695 14.838702600345744 6 31.172431461573137 36.90029304621471 17.445052122221778 14.482223369990368 7 28.26094402255593 33.36096196975281 20.39606449482751 17.982029512863758 8 26.546335963305516 34.65480416320167 19.81387660822639 18.98498326526642 9 26.886750447652414 16.717265888406796 20.02753045200906 36.36845321193174 10-11 24.841711888579418 27.12484193451602 27.05904696274518 20.974399214159376 12-13 25.97485131911897 24.499858888452007 27.596298356392968 21.928991436036053 14-15 22.81811406471129 25.787587389833668 26.43798489969435 24.956313645760694 16-17 21.872118687142 28.979088775603866 26.942475808959625 22.20631672829451 18-19 21.87023491647703 26.992652609399126 29.924844400543073 21.212268073580766 20-21 22.911651840820692 26.10436911492919 29.891142030828078 21.092837013422034 22-23 22.4215460893606 26.106081633715522 29.796747995325507 21.675624281598367 24-25 22.091560441653108 26.417567482867057 29.947833091924526 21.543038983555316 26-27 21.788058056319688 26.717991560713163 29.81956134753194 21.67438903543521 28-29 21.758099186348073 26.795182068147287 29.939640562856972 21.507078182647664 30-31 21.869841037156178 26.52170994315808 29.78020506384955 21.828243955836196 32-33 21.762294857374588 26.59000519235696 29.50681856479966 22.1408813854688 34-35 22.432533851892437 26.609922717954877 29.310058689002066 21.64748474115062 36-37 22.396250403298453 26.28579423593182 29.42826836146775 21.889686999301976 38-39 21.959769923998742 26.593075593317756 29.34373262792951 22.10342185475399 40-41 22.157506751082025 26.40813664655842 29.464328529367034 21.97002807299252 42-43 22.261458078238427 26.373556555289735 29.07186269657649 22.29312266989535 44-45 22.064952081147375 26.74129368143823 29.048896016012737 22.14485822140166 46-47 22.268464035050464 26.966331195653215 28.448430579283457 22.316774190012865 48-49 22.267487899342257 26.896768682552448 28.450006096566877 22.385737321538418 50-51 22.403564642104122 27.135425300615545 28.076539999643796 22.384470057636534 52-53 22.644293407898687 27.099119902345333 27.880781977178287 22.375804712577697 54-55 22.227808839062966 27.140271728780863 28.092260922102312 22.539658510053854 56-57 22.319394343755953 27.291144633856636 27.92328669345503 22.46617432893239 58-59 22.25974731442804 26.917901164375774 28.048266314481467 22.774085206714716 60-61 22.280503042118372 27.05252226616926 28.15906627995709 22.507908411755277 62-63 22.087108295578002 27.166370515084548 28.141256084579247 22.6052651047582 64-65 21.925720525154162 26.939273398829183 28.086455483416657 23.0485505926 66-67 21.966270891887245 27.076584577593255 28.042086205514728 22.91505832500478 68-69 22.24895488311183 26.9226836988481 27.979985268779693 22.848376149260382 70-71 22.122414673279504 26.95942647031373 27.848275552634377 23.06988330377239 72-73 22.271426692550815 26.94215043039022 27.706635941796282 23.079786935262682 74-75 22.13442518964433 27.21137550878933 27.49269096981994 23.1615083317464 76-77 22.080035592990455 26.9886966910027 27.827282890293308 23.10398482571354 78-79 22.155454920340475 27.10477121434022 27.7147019052799 23.0250719600394 80-81 22.04868680772739 27.313152541699477 27.652834714952967 22.985325935620168 82-83 22.233026385124415 27.242297167492136 27.45158548616935 23.073090961214103 84-85 22.143745126579212 27.070659014079467 27.47594608799927 23.309649771342052 86-87 22.25293219964252 27.070324668530045 27.344556641755226 23.332186490072214 88-89 22.12040723710687 27.034618579122675 27.30550489352411 23.539469290246345 90-91 21.96970734891865 27.00535281372085 27.576187171742845 23.448752665617658 92-93 21.9879533546989 26.769740629958854 27.57070964988624 23.671596365456008 94-95 21.797213115427876 26.80682719589434 27.610392386004474 23.78556730267331 96-97 21.94005430739575 26.685341113192013 27.51336107157096 23.86124350784128 98-99 21.829638572786354 26.68368910923305 27.305984398948514 24.180687919032085 100-101 21.886589470886495 26.54645583962056 27.509918908810434 24.057035780682515 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1467.0 1 980.0 2 606.0 3 747.5 4 677.0 5 662.0 6 1161.5 7 2594.0 8 3998.5 9 4328.5 10 3655.5 11 2967.0 12 3005.0 13 3320.0 14 3669.5 15 3930.0 16 4233.5 17 4595.0 18 4942.5 19 5321.0 20 5852.5 21 6526.0 22 7507.5 23 9055.0 24 10669.5 25 12877.0 26 16420.0 27 20755.5 28 24580.5 29 28859.0 30 33837.5 31 39395.0 32 46492.5 33 51287.0 34 55206.5 35 60045.0 36 65698.0 37 71800.0 38 75898.5 39 79259.5 40 83607.5 41 90244.0 42 96829.5 43 100942.5 44 103731.0 45 107862.0 46 109580.0 47 110531.0 48 113338.5 49 113143.5 50 110559.5 51 105784.5 52 101089.0 53 99393.5 54 97743.5 55 90993.0 56 80874.5 57 72724.5 58 66402.0 59 57795.0 60 47608.5 61 39449.5 62 32802.0 63 24993.0 64 18141.0 65 14149.0 66 11121.5 67 8864.5 68 6820.0 69 5020.5 70 3916.0 71 3194.5 72 2768.0 73 2542.0 74 2049.0 75 1702.0 76 1243.0 77 727.0 78 494.0 79 310.5 80 179.5 81 120.5 82 69.5 83 33.5 84 17.0 85 7.5 86 4.5 87 4.0 88 2.0 89 1.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.192578902590562E-4 2 0.04894378691334244 3 8.220090174389214E-4 4 0.0 5 0.08518068443210822 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.0825338153959547E-4 26-27 0.008048838295756105 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010429239408756315 36-37 6.850075145324343E-5 38-39 0.004075794711467984 40-41 0.0049320541046335275 42-43 3.7675413299283894E-4 44-45 1.7125187863310858E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.850075145324343E-5 68-69 9.076349567554756E-4 70-71 5.1375563589932585E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011987631504317602 82-83 6.678823266691236E-4 84-85 0.004812177789590351 86-87 0.0023119003615469664 88-89 3.4250375726621716E-5 90-91 0.003082533815395955 92-93 8.56259393165543E-4 94-95 0.0 96-97 0.0 98-99 3.4250375726621716E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2919676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.1135441258306 #Duplication Level Percentage of deduplicated Percentage of total 1 68.29947392268684 30.812313305821498 2 15.558062225549651 14.037586534495047 3 6.413811106695412 8.680492511299375 4 3.2673830876943653 5.896129244107694 5 1.8302321460057633 4.128412933967232 6 1.1140342099959062 3.015481889420109 7 0.7126758386800626 2.250593302699443 8 0.537496704176409 1.9398705025080756 9 0.37021418234039083 1.5031506465919366 >10 1.6937010139098259 13.352226348941965 >50 0.11630126923714312 3.641649653150145 >100 0.07901846489284031 6.6406535780228095 >500 0.005317079694891081 1.595699137291039 >1k 0.0020508735964716177 1.3737487160535633 >5k 7.595828135080066E-5 0.28212410335176696 >10k+ 1.519165627016013E-4 0.8498675922782915 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13948 0.47772424063491975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10853 0.3717193277610255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8233 0.2819833433572766 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.425037572662172E-5 0.0 0.0 0.0 0.0 3 3.425037572662172E-5 0.0 0.0 0.0 0.0 4 3.425037572662172E-5 0.0 0.0 0.0 0.0 5 1.370015029064869E-4 0.0 0.0 0.0 0.0 6 1.370015029064869E-4 0.0 0.0 0.0 3.425037572662172E-5 7 2.740030058129738E-4 0.0 0.0 0.0 3.425037572662172E-5 8 2.740030058129738E-4 0.0 0.0 0.0 3.425037572662172E-5 9 2.740030058129738E-4 0.0 0.0 0.0 3.425037572662172E-5 10-11 2.740030058129738E-4 0.0 0.0 0.0 3.425037572662172E-5 12-13 3.425037572662172E-4 0.0 0.0 0.0 3.425037572662172E-5 14-15 5.822563873525693E-4 0.0 0.0 0.0 3.425037572662172E-5 16-17 6.165067630791909E-4 0.0 0.0 0.0 3.425037572662172E-5 18-19 7.192578902590562E-4 0.0 0.0 5.1375563589932585E-5 3.425037572662172E-5 20-21 7.877586417122996E-4 0.0 0.0 2.740030058129738E-4 3.425037572662172E-5 22-23 8.220090174389214E-4 0.0 0.0 7.021327023957454E-4 3.425037572662172E-5 24-25 0.0010960120232518951 0.0 0.0 0.0015755172834245991 3.425037572662172E-5 26-27 0.0011816379625684493 0.0 0.0 0.0027571552459930487 3.425037572662172E-5 28-29 0.0014042654047914907 0.0 0.0 0.006404820260878262 3.425037572662172E-5 30-31 0.0015755172834245991 0.0 0.0 0.018615079207418905 3.425037572662172E-5 32-33 0.0016268928470145319 0.0 0.0 0.042230713270924584 3.425037572662172E-5 34-35 0.001832395101374262 0.0 0.0 0.07502544802916487 3.425037572662172E-5 36-37 0.0021920240465037903 0.0 0.0 0.1281991563447451 3.425037572662172E-5 38-39 0.002414651488726831 0.0 0.0 0.20918416975034215 3.425037572662172E-5 40-41 0.0024831522401800744 0.0 0.0 0.30054704700110557 3.425037572662172E-5 42-43 0.0028085308095829814 0.0 0.0 0.39249218063922164 3.425037572662172E-5 44-45 0.003322286445482307 0.0 0.0 0.5061349273001525 3.425037572662172E-5 46-47 0.003664790202748524 0.0 0.0 0.6400025208276534 3.425037572662172E-5 48-49 0.0039730435842881195 0.0 0.0 0.7836828470008317 3.425037572662172E-5 50-51 0.0039730435842881195 0.0 0.0 0.9414743279733779 3.425037572662172E-5 52-53 0.0039730435842881195 0.0 0.0 1.098426674740622 5.1375563589932585E-5 54-55 0.0039730435842881195 0.0 0.0 1.268719542853385 6.850075145324345E-5 56-57 0.004144295462921228 0.0 0.0 1.4635699303621361 6.850075145324345E-5 58-59 0.004144295462921228 0.0 0.0 1.6748262478439389 6.850075145324345E-5 60-61 0.004161420650784539 0.0 0.0 1.9116504708056647 6.850075145324345E-5 62-63 0.00417854583864785 0.0 0.0 2.146025791902937 6.850075145324345E-5 64-65 0.004247046590101094 0.0 0.0 2.386857308824678 6.850075145324345E-5 66-67 0.004418298468734202 0.0 0.0 2.664233976646724 6.850075145324345E-5 68-69 0.004452548844460824 0.0 0.0 2.9743882540391464 6.850075145324345E-5 70-71 0.004572425159504 0.0 0.0 3.307798536549946 6.850075145324345E-5 72-73 0.004692301474547175 0.0 0.0 3.657443497155164 6.850075145324345E-5 74-75 0.004726551850273797 0.0 0.0 4.021131111808296 6.850075145324345E-5 76-77 0.004726551850273797 0.0 0.0 4.4125101552364026 6.850075145324345E-5 78-79 0.004743677038137108 0.0 0.0 4.8340637796796635 6.850075145324345E-5 80-81 0.005462934928396165 0.0 0.0 5.2857919851380775 6.850075145324345E-5 82-83 0.005582811243439341 0.0 0.0 5.756049643864593 6.850075145324345E-5 84-85 0.005702687558482517 0.0 0.0 6.2288589555827425 6.850075145324345E-5 86-87 0.005839689061389004 0.0 0.0 6.725009898358585 6.850075145324345E-5 88-89 0.005891064624978936 0.0 0.0 7.264316314549971 6.850075145324345E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6915 0.0 57.160294 1 GTATCAA 13050 0.0 44.72184 1 TATCAAC 17565 0.0 33.198654 2 ATCAACG 17845 0.0 32.607662 3 TCAACGC 18135 0.0 32.08568 4 CAACGCA 18490 0.0 31.469646 5 AACGCAG 19185 0.0 30.375319 6 ACGCAGA 21985 0.0 26.48513 7 CGCAGAG 22175 0.0 26.129692 8 GCAGAGT 23665 0.0 23.9025 9 GTACATG 15845 0.0 23.534742 1 TACATGG 16015 0.0 23.046925 2 GAGTACT 12960 0.0 22.464334 12-13 TGGTATC 3085 0.0 22.047485 2 AGTACTT 13535 0.0 22.001251 12-13 ACATGGG 16415 0.0 21.604923 3 GTGGTAT 3345 0.0 21.329506 1 GTATAGG 650 0.0 21.221216 1 AGAGTAC 22175 0.0 20.978716 10-11 GTACTTT 13845 0.0 20.77109 14-15 >>END_MODULE