FastQCFastQC Report
Thu 26 May 2016
SRR938673_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938673_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2915427
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140980.48356552916605355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104090.3570317486941021No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78730.2700462059245524No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68200.054.5082631
GTATCAA127100.044.284981
TATCAAC167500.033.3672332
ATCAACG169650.032.944933
TCAACGC173550.032.2319834
CAACGCA175800.031.7383525
AACGCAG181000.030.8732196
ACGCAGA205200.027.0414057
CGCAGAG206650.026.7826928
GTACATG158250.025.448671
TACATGG158450.025.0450532
GCAGAGT221050.024.5643089
ACATGGG166450.022.9280413
GAGTACT116950.022.88786312-13
TGGTATC31950.021.4199942
AGAGTAC203650.021.41262210-11
AGTACTT121200.021.37980312-13
GTACTTT124800.021.18249314-15
CAGAGTA212600.021.05860910-11
GTGGTAT34300.020.5645731
Produced by FastQC (version 0.11.5)