FastQCFastQC Report
Thu 26 May 2016
SRR938673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2915427
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138420.47478465418616206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109880.3768916182775285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82160.2818112063858913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68050.057.737441
GTATCAA130850.045.4767231
ATCAACG175100.033.670323
TATCAAC175800.033.6594542
TCAACGC178000.033.201324
CAACGCA181050.032.51075
AACGCAG188050.031.3935176
ACGCAGA215400.027.253047
CGCAGAG216000.027.1993268
GCAGAGT230600.024.9211589
GAGTACT125200.022.98849112-13
TGGTATC30350.022.8823032
GTACATG156450.022.8029611
TACATGG158050.022.1808682
AGTACTT130250.022.15188612-13
GTGGTAT33600.021.6599641
AGAGTAC215300.021.62946310-11
GTACTTT131900.021.56871214-15
ACATGGG160400.021.1347353
CAGAGTA225150.020.95743410-11
Produced by FastQC (version 0.11.5)