##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938673_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2915427 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80302748105166 31.0 31.0 34.0 30.0 34.0 2 32.030277897542966 31.0 31.0 34.0 30.0 34.0 3 32.09180130389134 33.0 31.0 34.0 30.0 34.0 4 35.76772116057099 37.0 35.0 37.0 35.0 37.0 5 35.641212762315774 37.0 35.0 37.0 33.0 37.0 6 35.68429633120637 37.0 35.0 37.0 33.0 37.0 7 35.619387485949744 37.0 35.0 37.0 33.0 37.0 8 35.608156198045776 37.0 35.0 37.0 33.0 37.0 9 37.17140302261041 39.0 37.0 39.0 33.0 39.0 10-11 37.10997720059532 39.0 37.0 39.0 33.0 39.0 12-13 37.00262019937388 39.0 37.0 39.0 33.0 39.0 14-15 38.02632358827712 40.0 37.0 41.0 33.0 41.0 16-17 37.9585031626585 40.0 37.0 41.0 33.0 41.0 18-19 37.993599565346685 40.0 37.0 41.0 33.0 41.0 20-21 38.00124664414509 40.0 37.0 41.0 33.0 41.0 22-23 38.01477227864049 40.0 37.0 41.0 33.0 41.0 24-25 37.93887224753012 40.0 37.0 41.0 33.0 41.0 26-27 37.76024832726047 40.0 37.0 41.0 32.0 41.0 28-29 37.62207800092405 40.0 37.0 41.0 32.0 41.0 30-31 37.360124091599616 39.0 36.0 41.0 31.5 41.0 32-33 37.11994881024289 39.0 36.0 41.0 31.0 41.0 34-35 36.93234833182241 39.0 35.5 40.5 31.0 41.0 36-37 36.91747469581642 39.0 35.0 41.0 31.0 41.0 38-39 36.75866399673187 39.0 35.0 41.0 30.0 41.0 40-41 36.56722257151354 39.0 35.0 41.0 30.0 41.0 42-43 36.31637218150206 39.0 35.0 40.0 30.0 41.0 44-45 35.98881072995482 38.5 35.0 40.0 29.5 41.0 46-47 35.56739853201607 38.0 35.0 40.0 27.5 41.0 48-49 35.13277008822378 38.0 34.0 40.0 26.5 41.0 50-51 34.75915740644509 38.0 34.0 40.0 25.5 41.0 52-53 34.25916581001685 38.0 33.0 40.0 24.0 41.0 54-55 33.94400528636114 37.5 33.0 40.0 23.0 41.0 56-57 33.59114685430299 37.0 33.0 40.0 22.5 41.0 58-59 33.1534636264259 36.5 32.0 39.5 21.5 41.0 60-61 32.75771284961002 36.0 31.0 39.0 20.0 40.5 62-63 32.29492780988857 35.5 31.0 39.0 18.0 40.0 64-65 31.81500051278938 35.0 30.5 38.5 16.0 40.0 66-67 31.330273747207528 35.0 30.0 38.0 12.5 40.0 68-69 30.780066521988033 34.5 29.0 38.0 8.0 40.0 70-71 30.238485134424565 34.0 29.0 37.0 7.5 39.0 72-73 29.669676860370714 34.0 28.0 36.5 4.5 39.0 74-75 29.064292297491928 34.0 27.5 36.0 2.0 38.5 76-77 27.772245369203205 31.5 26.0 34.5 2.0 36.5 78-79 28.405172038264034 33.0 27.0 35.0 2.0 37.0 80-81 28.117710716131803 33.0 26.0 35.0 2.0 37.0 82-83 27.634223563135006 33.0 26.0 35.0 2.0 36.0 84-85 27.05371408716459 32.5 25.5 35.0 2.0 36.0 86-87 26.415784034379868 32.0 24.5 35.0 2.0 35.5 88-89 25.685899012391666 31.5 22.0 34.0 2.0 35.0 90-91 25.1532706872784 31.0 20.0 34.0 2.0 35.0 92-93 24.394617323637327 31.0 17.0 34.0 2.0 35.0 94-95 23.620421811281847 31.0 4.5 34.0 2.0 35.0 96-97 22.646032467971246 30.0 2.0 34.0 2.0 35.0 98-99 21.490291302097425 30.0 2.0 34.0 2.0 35.0 100-101 18.35366860497622 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2583538827302192 1101 2 0.32366582908749564 1101 3 0.24730503056873232 1101 4 0.24495745911259803 1101 5 0.168679675835385 1101 6 0.23209075102322174 1101 7 0.21738646361819036 1101 8 0.2474899520456546 1101 9 0.23867845648803865 1101 10-11 0.32718391727503615 1101 12-13 0.3549005135156875 1101 14-15 0.42892916401418546 1101 16-17 0.5214449758846271 1101 18-19 0.5881473927592964 1101 20-21 0.4980666489202221 1101 22-23 0.44952891575608334 1101 24-25 0.47505468371068815 1101 26-27 0.38587390181577064 1101 28-29 0.5011368238620193 1101 30-31 0.5492439247431946 1101 32-33 0.4485586445108609 1101 34-35 0.2761194618494045 1101 36-37 0.47426263709446204 1101 38-39 0.6094204716957634 1101 40-41 0.6881914457163418 1101 42-43 0.5467090411238829 1101 44-45 0.5627176360683563 1101 46-47 0.6214530283582391 1101 48-49 0.653324366254175 1101 50-51 0.7622739471476478 1101 52-53 0.8327459593021871 1101 54-55 0.7857243264698042 1101 56-57 0.8999225462859783 1101 58-59 1.1230058088109232 1101 60-61 1.1209484135543661 1101 62-63 1.1119424525751072 1101 64-65 1.0518842801390242 1101 66-67 1.1918814056883313 1101 68-69 1.2898405342530062 1101 70-71 1.2278800716336882 1101 72-73 1.3241663449027037 1101 74-75 1.4543203667461988 1101 76-77 1.417381832087532 1101 78-79 1.6170249567739603 1101 80-81 1.9413231245239793 1101 82-83 2.091470248577636 1101 84-85 2.2292255145713007 1101 86-87 2.2669725252648654 1101 88-89 2.4484745542476425 1101 90-91 2.8373130578672168 1101 92-93 2.9576510787591594 1101 94-95 3.1126851653077345 1101 96-97 3.52112337173579 1101 98-99 3.9115593379305444 1101 100-101 4.583221410830095 1102 1 0.3155021085590022 1102 2 0.36007523199388913 1102 3 0.2950725746047169 1102 4 0.281498925311098 1102 5 0.12000276574786994 1102 6 0.15730523521136774 1102 7 0.17412121686923854 1102 8 0.26298449417412684 1102 9 0.385811901279439 1102 10-11 0.407623933736879 1102 12-13 0.43869564956600726 1102 14-15 0.6943396658658614 1102 16-17 0.4859901002083902 1102 18-19 0.5036869843849772 1102 20-21 0.5919855670845351 1102 22-23 0.4236806673676483 1102 24-25 0.49514079847006087 1102 26-27 0.4986665630055853 1102 28-29 0.45284298108266796 1102 30-31 0.5388228606201864 1102 32-33 0.512865580262762 1102 34-35 0.6943506457619151 1102 36-37 0.8013714756745856 1102 38-39 0.8719543390896973 1102 40-41 0.7504569845122333 1102 42-43 0.8951849106755105 1102 44-45 0.9012473667820871 1102 46-47 0.8932572921319135 1102 48-49 1.1145933856018715 1102 50-51 1.1128318377757864 1102 52-53 1.2645020437065426 1102 54-55 1.205436755161216 1102 56-57 1.3504253360881293 1102 58-59 1.4058232576943297 1102 60-61 1.4439191759141465 1102 62-63 1.6413340735640674 1102 64-65 1.46599309303242 1102 66-67 1.5223197469287122 1102 68-69 1.6018253338518456 1102 70-71 1.859290306566752 1102 72-73 1.7318590595879186 1102 74-75 1.8121651217394792 1102 76-77 1.6900322921033428 1102 78-79 2.0256799465886104 1102 80-81 2.149382977953266 1102 82-83 2.1496257013118196 1102 84-85 2.3044389098654925 1102 86-87 2.4177001577078734 1102 88-89 2.5624028061875492 1102 90-91 2.8604392807788876 1102 92-93 3.188519002623689 1102 94-95 3.24614028724152 1102 96-97 3.7824105951567226 1102 98-99 4.277340245208155 1102 100-101 4.7945959691300395 1103 1 0.36483725604843187 1103 2 0.34042295606366935 1103 3 0.33310940781513665 1103 4 0.29093156340724136 1103 5 0.2690406382847428 1103 6 0.2546761692826962 1103 7 0.3378252757595348 1103 8 0.3733624244297218 1103 9 0.467694355972192 1103 10-11 0.45268798496812934 1103 12-13 0.5414530694725599 1103 14-15 0.5838351665802506 1103 16-17 0.5295752815712618 1103 18-19 0.5675605321879047 1103 20-21 0.5542588685646663 1103 22-23 0.4470312335612192 1103 24-25 0.4565112814363843 1103 26-27 0.5725220260413408 1103 28-29 0.5576616100966731 1103 30-31 0.6012392418980284 1103 32-33 0.6648506451172267 1103 34-35 0.6623550497150887 1103 36-37 0.7134264861449253 1103 38-39 0.797440879365837 1103 40-41 0.809720093128746 1103 42-43 0.8901266118444013 1103 44-45 0.9408310195805214 1103 46-47 1.0433306372621374 1103 48-49 1.1009427940133563 1103 50-51 1.0909426976419638 1103 52-53 1.2818517368983464 1103 54-55 1.2760237352647295 1103 56-57 1.290864871385061 1103 58-59 1.5647720532196274 1103 60-61 1.3395843083369883 1103 62-63 1.5152100635002057 1103 64-65 1.5510012348286786 1103 66-67 1.5668483138021791 1103 68-69 1.6328016841040487 1103 70-71 1.5872052278383784 1103 72-73 1.6598351964577205 1103 74-75 1.810075718569248 1103 76-77 1.6583675199154264 1103 78-79 1.9361195792463448 1103 80-81 2.294459686087521 1103 82-83 2.473327821099673 1103 84-85 2.4831539745820237 1103 86-87 2.744197343575305 1103 88-89 2.9997967741996625 1103 90-91 3.208423182171881 1103 92-93 3.217190741787636 1103 94-95 3.3804383443257784 1103 96-97 3.8311070152815283 1103 98-99 3.966490358511777 1103 100-101 4.234629834458968 1104 1 0.2358595888823345 1104 2 0.22369407032928734 1104 3 0.23547187129760516 1104 4 0.18475531709468385 1104 5 0.16049787488113054 1104 6 0.22842675915922683 1104 7 0.22247844110520987 1104 8 0.2030802703268222 1104 9 0.2827520332136757 1104 10-11 0.3103134600880182 1104 12-13 0.2984438234208042 1104 14-15 0.41846450741505237 1104 16-17 0.5081248086375183 1104 18-19 0.4072845451788325 1104 20-21 0.47901618105785815 1104 22-23 0.36125082085287374 1104 24-25 0.47325854862859273 1104 26-27 0.3974114887240461 1104 28-29 0.5438692706010855 1104 30-31 0.5186504085954127 1104 32-33 0.5924520484306299 1104 34-35 0.49294837309031436 1104 36-37 0.5552003121217268 1104 38-39 0.5436285114745942 1104 40-41 0.5435395025715124 1104 42-43 0.5181166262003032 1104 44-45 0.6522225010367961 1104 46-47 0.6965248553376071 1104 48-49 0.7767821523092735 1104 50-51 0.770758097836385 1104 52-53 0.9546371855142652 1104 54-55 0.9997481296517776 1104 56-57 1.0698037488312195 1104 58-59 1.1687217466622926 1104 60-61 1.1628081585462127 1104 62-63 1.2824583592637282 1104 64-65 1.355539415360095 1104 66-67 1.488791937539606 1104 68-69 1.545211041974202 1104 70-71 1.560479518705442 1104 72-73 1.7574890493871926 1104 74-75 1.7288874835180756 1104 76-77 1.674876223319007 1104 78-79 1.886760202370116 1104 80-81 2.141668151959834 1104 82-83 2.1799799585113924 1104 84-85 2.4430502735863904 1104 86-87 2.674718811002009 1104 88-89 2.5480280241566398 1104 90-91 2.5894476667325996 1104 92-93 2.9406129638738925 1104 94-95 3.1298617886132583 1104 96-97 3.5064285824423003 1104 98-99 3.834507794637485 1104 100-101 4.321210197504232 1105 1 0.2343763938272332 1105 2 0.26038048549706616 1105 3 0.17210186332913935 1105 4 0.1176952941090974 1105 5 0.13567230007194553 1105 6 0.2222890789610119 1105 7 0.2359896158392587 1105 8 0.2307849742396968 1105 9 0.21644165610089772 1105 10-11 0.19780195034915948 1105 12-13 0.31251699050397264 1105 14-15 0.4415415943449261 1105 16-17 0.40047546765781306 1105 18-19 0.439274720630344 1105 20-21 0.26273578251832674 1105 22-23 0.30356451799885065 1105 24-25 0.3251067270641599 1105 26-27 0.37457246337503136 1105 28-29 0.4856594017080269 1105 30-31 0.4815077793305136 1105 32-33 0.48098978875542997 1105 34-35 0.5733495873375034 1105 36-37 0.5634272943383962 1105 38-39 0.5303927958956081 1105 40-41 0.6582508731768755 1105 42-43 0.6829223500382184 1105 44-45 0.6731527977677843 1105 46-47 0.8382881666955271 1105 48-49 0.9954327582710008 1105 50-51 0.9542497798413052 1105 52-53 0.9656171254070429 1105 54-55 0.93457613953926 1105 56-57 1.0547718604401055 1105 58-59 1.1462855621734036 1105 60-61 1.1887909401705201 1105 62-63 1.3090824669261423 1105 64-65 1.3360926162535627 1105 66-67 1.3604898769695772 1105 68-69 1.3743630078037192 1105 70-71 1.3392653634936487 1105 72-73 1.3849178342395518 1105 74-75 1.583461933011261 1105 76-77 1.4596803544317538 1105 78-79 1.7882512400232997 1105 80-81 1.9645173557424798 1105 82-83 2.0371250978917885 1105 84-85 2.2760071702800104 1105 86-87 2.3500698035747796 1105 88-89 2.542103050226018 1105 90-91 2.803997489499565 1105 92-93 3.0361338116297 1105 94-95 3.040210363051621 1105 96-97 3.420821541144729 1105 98-99 3.764475330154287 1105 100-101 4.271045769205426 1106 1 0.16517876565196232 1106 2 0.18281438724114452 1106 3 0.19166569309631143 1106 4 0.15132945834164957 1106 5 0.09008449183184553 1106 6 0.07202314909748253 1106 7 0.14333113748041626 1106 8 0.05477909842012707 1106 9 0.21002888543323195 1106 10-11 0.2588819056205196 1106 12-13 0.1677998303199999 1106 14-15 0.09946178804332106 1106 16-17 0.1800907485600831 1106 18-19 0.2920051570362787 1106 20-21 0.4125049501168334 1106 22-23 0.2731895385057612 1106 24-25 0.18363838030783342 1106 26-27 0.23021723840241748 1106 28-29 0.3721036728782323 1106 30-31 0.47075779677317087 1106 32-33 0.4121135533907676 1106 34-35 0.4583481212111522 1106 36-37 0.5159695857194606 1106 38-39 0.4849553716769037 1106 40-41 0.5501458579876228 1106 42-43 0.7336681180715701 1106 44-45 0.3342989408654944 1106 46-47 0.17639599628167701 1106 48-49 0.5317508530044464 1106 50-51 0.3776353673006838 1106 52-53 0.6975105828064088 1106 54-55 0.8041163510008786 1106 56-57 0.742026838198349 1106 58-59 1.1043888802389077 1106 60-61 0.8807751182629175 1106 62-63 1.0661996618068628 1106 64-65 0.735849762558832 1106 66-67 0.837261556311244 1106 68-69 0.7546190236359962 1106 70-71 0.8350840748487265 1106 72-73 1.4002619543657424 1106 74-75 1.5572961739915208 1106 76-77 1.658697460496331 1106 78-79 1.905795209671183 1106 80-81 1.8133636180159876 1106 82-83 2.1720822643739695 1106 84-85 2.0579321894669356 1106 86-87 2.3281723107922687 1106 88-89 2.7397801356611104 1106 90-91 2.559504538220221 1106 92-93 2.259764869182934 1106 94-95 2.7357183121907056 1106 96-97 3.1453495993145886 1106 98-99 3.6875819528968776 1106 100-101 4.27519924387774 1107 1 0.1870101333484513 1107 2 0.1934668311251997 1107 3 0.1318859651444697 1107 4 0.1342029880262814 1107 5 0.07778654666847018 1107 6 0.07982231768395565 1107 7 0.11423457033075124 1107 8 0.14007544178787157 1107 9 0.18251723524840457 1107 10-11 0.18554649319447236 1107 12-13 0.23277902262601913 1107 14-15 0.37850196259768154 1107 16-17 0.45070221419748435 1107 18-19 0.37683174840849887 1107 20-21 0.24965811329990828 1107 22-23 0.22406734039059018 1107 24-25 0.21843652387924095 1107 26-27 0.2181379333112119 1107 28-29 0.25654021080881506 1107 30-31 0.313763270599523 1107 32-33 0.3513263038180412 1107 34-35 0.44650383184168874 1107 36-37 0.39502580058587 1107 38-39 0.4521810460127398 1107 40-41 0.484653711033765 1107 42-43 0.5947929407618489 1107 44-45 0.49454680001478835 1107 46-47 0.5486733628983771 1107 48-49 0.6120455811630734 1107 50-51 0.5312703143900137 1107 52-53 0.6689673225878394 1107 54-55 0.7345060914608084 1107 56-57 0.7106986556495443 1107 58-59 0.8001343970555297 1107 60-61 0.8481884698386111 1107 62-63 0.9502116624194059 1107 64-65 0.9921279518702839 1107 66-67 1.1818991738051317 1107 68-69 1.245694182837493 1107 70-71 1.0276943533912615 1107 72-73 1.3376220766968778 1107 74-75 1.3444019986927067 1107 76-77 1.3290650798569352 1107 78-79 1.5991743053999912 1107 80-81 1.7452196218433045 1107 82-83 1.8968633727986557 1107 84-85 2.111494368311387 1107 86-87 2.309251617001266 1107 88-89 2.580777720113076 1107 90-91 2.4503010319309624 1107 92-93 2.6141824381870187 1107 94-95 2.865712298470932 1107 96-97 3.131692866463471 1107 98-99 3.219471714035951 1107 100-101 3.4568609196041074 1108 1 0.20755453147905456 1108 2 0.21064088053186225 1108 3 0.12364120970509163 1108 4 0.15157463839033625 1108 5 0.06293008032164948 1108 6 0.038217147089888215 1108 7 0.10964339879043195 1108 8 0.0845728010426825 1108 9 0.10874068061269782 1108 10-11 0.07427863578401173 1108 12-13 0.22263939603402605 1108 14-15 0.32925029861778654 1108 16-17 0.24269675287524706 1108 18-19 0.3177490621487138 1108 20-21 0.3295475595248263 1108 22-23 0.306084047626463 1108 24-25 0.3071528776598882 1108 26-27 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64-65 0.1247295321923012 2316 66-67 0.37768387972637996 2316 68-69 0.31515120887864256 2316 70-71 0.4634929449871201 2316 72-73 0.6528566454144347 2316 74-75 0.8000846559259323 2316 76-77 0.8070606937470153 2316 78-79 0.8774167757276246 2316 80-81 1.0696077154283437 2316 82-83 1.3006565864506996 2316 84-85 1.421937277250013 2316 86-87 1.4875789442293126 2316 88-89 1.6274424031119388 2316 90-91 1.7571634204874051 2316 92-93 1.5209676841067186 2316 94-95 2.156864038037387 2316 96-97 2.3151832401644405 2316 98-99 2.519154327187671 2316 100-101 2.9665357082883865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 53.0 5 107.0 6 237.0 7 621.0 8 1441.0 9 2550.0 10 4184.0 11 6492.0 12 9312.0 13 13176.0 14 16926.0 15 22709.0 16 28962.0 17 36949.0 18 33988.0 19 27027.0 20 26304.0 21 27664.0 22 30326.0 23 34436.0 24 40077.0 25 46036.0 26 54902.0 27 65929.0 28 78366.0 29 93550.0 30 110759.0 31 131316.0 32 156739.0 33 191145.0 34 238211.0 35 304839.0 36 384658.0 37 435471.0 38 245865.0 39 14078.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.16692248985997 19.82091804796899 15.763739420496153 22.248420041674883 2 15.788104677302407 21.267668609952107 39.3341836708432 23.610043041902284 3 18.817520381310935 25.48044601525134 30.27122029926432 25.430813304173398 4 13.114271082760776 17.887294039603805 36.80256785712693 32.19586702050849 5 13.725364299350373 37.837414676353845 33.58822842702339 14.848992597272389 6 31.152932314889036 36.83299221692054 17.512803441828588 14.501272026361834 7 28.227117331354894 33.398400988946044 20.38847139715726 17.986010282541802 8 26.526165806929825 34.68202084977604 19.813324085974372 18.97848925731977 9 26.840425090389846 16.657251236268305 20.05325463474133 36.44906903860053 10-11 24.81970908549588 27.144960240815497 27.032215178085405 21.003115495603218 12-13 26.001114073513076 24.471904115589243 27.57560041805197 21.951381392845715 14-15 22.809043066418745 25.804847797595343 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22.074570224225244 40-41 22.204144582016315 26.41671499090887 29.408512990602542 21.970627436472274 42-43 22.27779761592366 26.374466213808105 29.074552209597844 22.27318396067039 44-45 22.079862767810805 26.71120904481141 29.03756700308128 22.171361184296504 46-47 22.28724986082656 26.923569000355695 28.430380867022226 22.35880027179552 48-49 22.286049350575404 26.90439513663007 28.403489437396306 22.40606607539822 50-51 22.423936527994012 27.148887624351424 28.09399789464802 22.33317795300654 52-53 22.641263183746325 27.105377702820206 27.876756989627932 22.376602123805537 54-55 22.213041177158612 27.150379687092148 28.056250422322353 22.580328713426884 56-57 22.32268206338214 27.29210849731446 27.932580716306738 22.452628722996664 58-59 22.284934591056473 26.875977343970543 28.052065786589754 22.787022278383233 60-61 22.293664015597027 27.02650417931919 28.137816518815256 22.54201528626853 62-63 22.11297007265145 27.125820677382762 28.110033281574193 22.651175968391595 64-65 21.9437324275312 26.94013604182166 28.07103384855803 23.045097682089107 66-67 21.981803311048957 27.092746998246053 28.012082648111996 22.913367042593 68-69 22.23525826368503 26.895699301113062 27.98278053447846 22.886261900723444 70-71 22.117703513070296 26.946395845274125 27.85350139104838 23.0823992506072 72-73 22.242011589041947 26.967632351561598 27.676345124804207 23.114010934592248 74-75 22.149328383115062 27.240983910761614 27.411713618622592 23.197974087500732 76-77 22.090794933297936 26.9876076471817 27.793441578197637 23.12815584132273 78-79 22.14171371809344 27.10896208342723 27.673081850445918 23.07624234803341 80-81 22.02412271825798 27.336125149463577 27.604679384762292 23.035072747516143 82-83 22.290733833618827 27.20593935244608 27.455441822431347 23.047884991503746 84-85 22.17932587291308 27.02017222264909 27.465410030405145 23.33509187403269 86-87 22.29797925661683 27.03793235033079 27.32964314349293 23.334445249559447 88-89 22.107743069496006 27.049490014582773 27.30379242968396 23.53897448623726 90-91 21.994161704092278 27.035741683275276 27.54140033007415 23.428696282558292 92-93 22.036514433156775 26.79110832134965 27.524706967858002 23.647670277635573 94-95 21.825036263984657 26.836977910954378 27.527339905955454 23.810645919105504 96-97 22.012470214483162 26.702092009163664 27.4811545615788 23.804283214774372 98-99 21.839373786412764 26.720116813077468 27.259265966872093 24.181243433637682 100-101 21.930221274839845 26.498888746541425 27.522555641135533 24.04833433748319 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1419.0 1 933.5 2 588.5 3 751.5 4 678.0 5 671.5 6 1140.0 7 2556.0 8 3980.5 9 4390.5 10 3727.0 11 3000.5 12 3082.0 13 3362.5 14 3640.5 15 3959.0 16 4305.5 17 4569.0 18 4941.5 19 5348.5 20 5782.5 21 6640.5 22 7719.5 23 9084.0 24 10699.5 25 12871.5 26 16339.0 27 20656.0 28 24580.0 29 28898.5 30 33788.0 31 39204.0 32 46190.5 33 51240.5 34 54815.0 35 59201.0 36 65121.0 37 71707.0 38 75803.5 39 78901.0 40 83160.5 41 89944.0 42 96381.5 43 100654.5 44 103790.0 45 107791.0 46 109282.0 47 110312.5 48 113425.0 49 112851.0 50 110031.0 51 105569.0 52 100747.5 53 98780.5 54 97585.5 55 90895.0 56 80509.0 57 72436.5 58 66406.5 59 58121.0 60 48000.5 61 39862.0 62 32975.5 63 25182.0 64 18400.0 65 14264.0 66 11234.0 67 9026.5 68 7037.5 69 5167.0 70 4089.5 71 3371.0 72 2870.5 73 2575.5 74 2155.5 75 1732.0 76 1233.5 77 750.0 78 464.5 79 293.0 80 196.5 81 129.5 82 76.5 83 40.5 84 18.0 85 10.5 86 7.0 87 6.5 88 3.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.860058578040198E-5 2 0.044247377828359274 3 3.773032217922109E-4 4 0.0 5 0.10245497486303036 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013891618620531401 26-27 0.012176603976021352 28-29 0.0 30-31 0.0 32-33 5.488046862432159E-4 34-35 0.016086837365504262 36-37 0.003910233389482913 38-39 0.013359964080733285 40-41 0.0076661154609599215 42-43 0.005762449205553766 44-45 0.002606822259655275 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2295190378630641E-4 68-69 1.8865161089610545E-4 70-71 0.0 72-73 1.7150146445100496E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.430029289020099E-5 82-83 8.575073222550247E-4 84-85 0.00449333836861633 86-87 0.001354861569162939 88-89 1.7150146445100496E-5 90-91 7.374562971393213E-4 92-93 1.5435131800590443E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.746574687001252E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2915427.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.90331479071587 #Duplication Level Percentage of deduplicated Percentage of total 1 67.9822939617282 30.526303459584636 2 15.637195684845324 14.043238405612666 3 6.561179740638351 8.83856157877054 4 3.27436572929651 5.881195003301324 5 1.8537624831118888 4.162004016319612 6 1.1562925275668867 3.115282041329305 7 0.7427254094557058 2.334558300469702 8 0.5054833021423417 1.815830067003849 9 0.3761812756204138 1.520260761380084 >10 1.7075262680358032 13.37661025940195 >50 0.11700208242401064 3.6626683283490196 >100 0.07856428114347534 6.676931574753881 >500 0.005206734756895588 1.58048563276765 >1k 0.001990810348119508 1.3298730374903651 >5k 7.656962877382724E-5 0.2824849886507107 >10k+ 1.5313925754765448E-4 0.8537125448146478 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13842 0.47478465418616206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10988 0.3768916182775285 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8216 0.2818112063858913 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.860058578040198E-5 0.0 0.0 0.0 0.0 3 6.860058578040198E-5 0.0 0.0 0.0 0.0 4 6.860058578040198E-5 0.0 0.0 0.0 0.0 5 1.3720117156080396E-4 0.0 0.0 0.0 3.430029289020099E-5 6 1.7150146445100496E-4 0.0 0.0 0.0 3.430029289020099E-5 7 2.4010205023140694E-4 0.0 0.0 0.0 3.430029289020099E-5 8 2.4010205023140694E-4 0.0 0.0 0.0 3.430029289020099E-5 9 2.4010205023140694E-4 0.0 0.0 0.0 3.430029289020099E-5 10-11 2.5725219667650746E-4 0.0 0.0 0.0 3.430029289020099E-5 12-13 3.430029289020099E-4 0.0 0.0 0.0 3.430029289020099E-5 14-15 4.802041004628139E-4 0.0 0.0 0.0 3.430029289020099E-5 16-17 5.145043933530149E-4 0.0 0.0 0.0 3.430029289020099E-5 18-19 6.688557113589194E-4 0.0 0.0 1.7150146445100496E-5 3.430029289020099E-5 20-21 7.889067364746227E-4 0.0 0.0 1.2005102511570347E-4 3.430029289020099E-5 22-23 7.889067364746227E-4 0.0 0.0 6.345554184687183E-4 3.430029289020099E-5 24-25 9.604082009256277E-4 3.430029289020099E-5 0.0 0.001423462154943341 3.430029289020099E-5 26-27 9.604082009256277E-4 3.430029289020099E-5 0.0 0.002881224602776883 3.430029289020099E-5 28-29 0.0013548615691629392 3.430029289020099E-5 0.0 0.006002551255785173 3.430029289020099E-5 30-31 0.0016464140587296476 3.430029289020099E-5 0.0 0.017578900106228007 3.430029289020099E-5 32-33 0.0016464140587296476 3.430029289020099E-5 0.0 0.039616838288182146 3.430029289020099E-5 34-35 0.001835065669625753 3.430029289020099E-5 0.0 0.07559784553000298 3.430029289020099E-5 36-37 0.0022295190378630644 3.430029289020099E-5 0.0 0.13128437103724427 3.430029289020099E-5 38-39 0.002332419916533667 3.430029289020099E-5 0.0 0.21130695435008318 3.430029289020099E-5 40-41 0.0023495700629787676 3.430029289020099E-5 0.0 0.30168822611576285 3.430029289020099E-5 42-43 0.0026411225525454765 3.430029289020099E-5 0.0 0.3923095999316738 3.430029289020099E-5 44-45 0.002949825188557285 3.430029289020099E-5 0.0 0.5034082486030348 3.430029289020099E-5 46-47 0.0033099782639043956 3.430029289020099E-5 0.0 0.6363390336990087 3.430029289020099E-5 48-49 0.0034643295819103 3.430029289020099E-5 0.0 0.775975526055017 3.430029289020099E-5 50-51 0.0034643295819103 3.430029289020099E-5 0.0 0.927874373119272 3.430029289020099E-5 52-53 0.0034643295819103 3.430029289020099E-5 0.0 1.0817454870247136 3.430029289020099E-5 54-55 0.0034986298748005008 3.430029289020099E-5 0.0 1.2534184529401697 3.430029289020099E-5 56-57 0.0037387319250319078 3.430029289020099E-5 0.0 1.4468206544015678 3.430029289020099E-5 58-59 0.0037387319250319078 3.430029289020099E-5 0.0 1.6628267488776087 3.430029289020099E-5 60-61 0.0037387319250319078 3.430029289020099E-5 0.0 1.9009908325607192 3.430029289020099E-5 62-63 0.0037387319250319078 3.430029289020099E-5 0.0 2.1380573068713433 3.430029289020099E-5 64-65 0.0037387319250319078 3.430029289020099E-5 0.0 2.3856539710992593 3.430029289020099E-5 66-67 0.003910233389482913 3.430029289020099E-5 0.0 2.6627831875056382 3.430029289020099E-5 68-69 0.003944533682373114 3.430029289020099E-5 0.0 2.96941065579759 3.430029289020099E-5 70-71 0.003978833975263315 3.430029289020099E-5 0.0 3.307971696770319 3.430029289020099E-5 72-73 0.003995984121708415 3.430029289020099E-5 0.0 3.6563597716560903 3.430029289020099E-5 74-75 0.004047434561043717 3.430029289020099E-5 0.0 4.0198742757064405 3.430029289020099E-5 76-77 0.004133185293269219 3.430029289020099E-5 0.0 4.407621936683718 3.430029289020099E-5 78-79 0.00418463573260452 3.430029289020099E-5 0.0 4.829052485279172 6.860058578040198E-5 80-81 0.004904941883298742 3.430029289020099E-5 0.0 5.282330855823178 6.860058578040198E-5 82-83 0.004956392322634043 3.430029289020099E-5 0.0 5.752879423837401 6.860058578040198E-5 84-85 0.0050764433477497465 3.430029289020099E-5 0.0 6.234901439823394 8.575073222550248E-5 86-87 0.00519649437286545 3.430029289020099E-5 0.0 6.731689731898621 1.0290087867060297E-4 88-89 0.00521364451931055 3.430029289020099E-5 0.0 7.2597941913826 1.0290087867060297E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6805 0.0 57.73744 1 GTATCAA 13085 0.0 45.476723 1 ATCAACG 17510 0.0 33.67032 3 TATCAAC 17580 0.0 33.659454 2 TCAACGC 17800 0.0 33.20132 4 CAACGCA 18105 0.0 32.5107 5 AACGCAG 18805 0.0 31.393517 6 ACGCAGA 21540 0.0 27.25304 7 CGCAGAG 21600 0.0 27.199326 8 GCAGAGT 23060 0.0 24.921158 9 GAGTACT 12520 0.0 22.988491 12-13 TGGTATC 3035 0.0 22.882303 2 GTACATG 15645 0.0 22.802961 1 TACATGG 15805 0.0 22.180868 2 AGTACTT 13025 0.0 22.151886 12-13 GTGGTAT 3360 0.0 21.659964 1 AGAGTAC 21530 0.0 21.629463 10-11 GTACTTT 13190 0.0 21.568712 14-15 ACATGGG 16040 0.0 21.134735 3 CAGAGTA 22515 0.0 20.957434 10-11 >>END_MODULE