FastQCFastQC Report
Thu 26 May 2016
SRR938672_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938672_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897433
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133100.45937214078807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97940.3380233468729044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73100.25229228769051776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65700.054.3464241
GTATCAA127950.046.1248441
TATCAAC167150.035.171882
ATCAACG170100.034.6177833
TCAACGC172900.034.0021934
CAACGCA176600.033.3436285
AACGCAG183550.032.1008876
ACGCAGA209250.027.992067
CGCAGAG211150.027.6951838
GCAGAGT223150.025.6736539
GTACATG157850.024.9151731
TACATGG158750.024.249542
GAGTACT117600.022.72132912-13
AGTACTT121150.022.54565812-13
ACATGGG166700.022.209263
AGAGTAC206500.021.99176610-11
TCGCGTA4600.021.6869629
GTACTTT126200.021.06149714-15
CAGAGTA216500.020.855310-11
CATGGGA97300.020.4660224
Produced by FastQC (version 0.11.5)