FastQCFastQC Report
Thu 26 May 2016
SRR938672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897433
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133140.4595101940234683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106730.36836054535169577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77110.26613212453920415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67900.054.022811
GTATCAA128650.045.0218731
TATCAAC173250.033.2883762
ATCAACG173600.033.07933
TCAACGC176600.032.544264
CAACGCA180400.031.8587365
AACGCAG186950.030.8436286
ACGCAGA213850.026.8972057
CGCAGAG215150.026.62438
GTACATG158750.024.5431181
GCAGAGT227150.024.5068269
TACATGG158750.024.239212
ACATGGG161700.022.8239463
GAGTACT124550.022.51896312-13
TGGTATC31500.022.4710272
AGAGTAC213450.021.4957810-11
AGTACTT129600.021.45824212-13
GTACTTT131650.021.12410414-15
CAGAGTA222600.020.3774810-11
GTATAGG6350.020.2031781
Produced by FastQC (version 0.11.5)