##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938672_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2897433 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81118044834859 31.0 31.0 34.0 30.0 34.0 2 32.06091357418791 33.0 31.0 34.0 30.0 34.0 3 32.11186177557859 33.0 31.0 34.0 30.0 34.0 4 35.77339458755388 37.0 35.0 37.0 35.0 37.0 5 35.66750948166877 37.0 35.0 37.0 33.0 37.0 6 35.70352998671583 37.0 35.0 37.0 33.0 37.0 7 35.62675754711153 37.0 35.0 37.0 33.0 37.0 8 35.602285195205546 37.0 35.0 37.0 33.0 37.0 9 37.138287925898545 39.0 37.0 39.0 33.0 39.0 10-11 37.07140268644693 39.0 37.0 39.0 33.0 39.0 12-13 36.95755812127493 39.0 37.0 39.0 33.0 39.0 14-15 37.94018964372947 40.0 37.0 41.0 33.0 41.0 16-17 38.039425242965066 40.0 37.0 41.0 33.0 41.0 18-19 38.2039702039702 40.0 37.5 41.0 33.0 41.0 20-21 38.169664665239885 40.0 37.5 41.0 33.0 41.0 22-23 38.04060508042809 40.0 37.0 41.0 33.0 41.0 24-25 37.91681136371402 40.0 37.0 41.0 33.0 41.0 26-27 37.69729602030487 40.0 37.0 41.0 32.0 41.0 28-29 37.54521053636098 39.5 36.5 41.0 32.0 41.0 30-31 37.269378273803056 39.0 36.0 41.0 31.0 41.0 32-33 37.13441553264562 39.0 36.0 41.0 31.0 41.0 34-35 36.98102164916324 39.0 36.0 41.0 31.0 41.0 36-37 36.83733031963121 39.0 35.0 41.0 30.5 41.0 38-39 36.613790724410194 39.0 35.0 40.5 30.0 41.0 40-41 36.376064433586556 39.0 35.0 40.0 30.0 41.0 42-43 36.08644082537887 38.5 35.0 40.0 30.0 41.0 44-45 35.74425223982746 38.0 35.0 40.0 28.5 41.0 46-47 35.258510377979405 38.0 34.0 40.0 26.5 41.0 48-49 34.803468794619235 38.0 34.0 40.0 25.5 41.0 50-51 34.3959642897696 38.0 33.0 40.0 24.0 41.0 52-53 33.81215631215631 37.5 33.0 40.0 23.0 41.0 54-55 33.39758831351752 37.0 32.0 40.0 22.0 41.0 56-57 33.01800714632573 37.0 32.0 39.0 20.0 40.5 58-59 32.4986544986545 36.0 31.0 39.0 18.5 40.0 60-61 32.025148467626344 36.0 31.0 39.0 16.0 40.0 62-63 31.539884615105855 35.0 30.0 38.5 12.0 40.0 64-65 30.91198295180596 35.0 29.0 38.0 8.0 40.0 66-67 30.27887754436427 34.0 28.5 38.0 7.0 40.0 68-69 29.56525379534229 34.0 27.5 37.0 2.0 39.0 70-71 28.80993693383074 33.5 26.0 36.0 2.0 39.0 72-73 28.252037924604295 33.0 26.0 36.0 2.0 39.0 74-75 27.552352547927768 32.0 25.5 35.0 2.0 38.0 76-77 26.394714907989243 30.5 24.0 34.0 2.0 36.0 78-79 26.90473532951409 32.0 25.0 35.0 2.0 37.0 80-81 26.53675563852555 32.0 24.0 35.0 2.0 36.0 82-83 25.8732761033646 31.5 22.0 34.5 2.0 36.0 84-85 25.103193758061014 31.0 19.5 34.0 2.0 35.0 86-87 24.21029770144814 30.5 15.5 34.0 2.0 35.0 88-89 23.254940839011635 30.0 7.0 34.0 2.0 35.0 90-91 22.406309826663808 29.0 2.0 34.0 2.0 35.0 92-93 21.337712899659802 29.0 2.0 33.5 2.0 35.0 94-95 20.16680282167008 27.0 2.0 33.0 2.0 34.5 96-97 18.79218535855704 25.5 2.0 32.5 2.0 34.0 98-99 17.017447513022734 19.0 2.0 32.0 2.0 34.0 100-101 12.742117936808203 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2695930758155036 1101 2 0.23335214369009805 1101 3 0.28440266199235253 1101 4 0.20698492561261617 1101 5 0.13632812313959164 1101 6 0.2166414756609285 1101 7 0.1770699549237733 1101 8 0.22917726404416783 1101 9 0.299216237888551 1101 10-11 0.3682668609142894 1101 12-13 0.31991509274877217 1101 14-15 0.41335760547764266 1101 16-17 0.27033124409544484 1101 18-19 0.31936541012341024 1101 20-21 0.2666834653638688 1101 22-23 0.39436545348130636 1101 24-25 0.27565855276082374 1101 26-27 0.4264672867883732 1101 28-29 0.5093931569452863 1101 30-31 0.4628261124945965 1101 32-33 0.3018930975595282 1101 34-35 0.18890321340037275 1101 36-37 0.3980449038136342 1101 38-39 0.4336806373762059 1101 40-41 0.4907070977027814 1101 42-43 0.5174718947719299 1101 44-45 0.6207425475224042 1101 46-47 0.5210921878021537 1101 48-49 0.5553495794908585 1101 50-51 0.5831795654192149 1101 52-53 0.8452333021229421 1101 54-55 0.8228515816294788 1101 56-57 1.0466786543374837 1101 58-59 1.0828721393148868 1101 60-61 1.018594485842705 1101 62-63 0.9828176355678266 1101 64-65 1.1093062278307535 1101 66-67 1.1194988872616491 1101 68-69 1.3461232975310011 1101 70-71 1.3697796955805117 1101 72-73 1.7038077498494282 1101 74-75 1.5228216679177926 1101 76-77 1.611784615517049 1101 78-79 1.612979592154236 1101 80-81 1.713635574437287 1101 82-83 2.0801561870564633 1101 84-85 2.1883636304123613 1101 86-87 2.2348238561790446 1101 88-89 2.228333035659432 1101 90-91 2.5385904557575962 1101 92-93 2.7219383282279743 1101 94-95 2.5460840645522147 1101 96-97 2.293344166224358 1101 98-99 2.2108540755040096 1101 100-101 2.3173253566639342 1102 1 0.26861114922504115 1102 2 0.29072082877215166 1102 3 0.22570271237869832 1102 4 0.17655430727342747 1102 5 0.18357097481887052 1102 6 0.18994844216587836 1102 7 0.243172088534223 1102 8 0.2461456784858953 1102 9 0.21999392595570555 1102 10-11 0.24863386166870782 1102 12-13 0.33958701309751405 1102 14-15 0.46352002278582205 1102 16-17 0.36414372688378194 1102 18-19 0.36230058834370027 1102 20-21 0.34444537362099226 1102 22-23 0.39879240128866655 1102 24-25 0.32209131845219474 1102 26-27 0.3991369047075892 1102 28-29 0.3376281614899952 1102 30-31 0.32268868692206354 1102 32-33 0.3503701524585665 1102 34-35 0.5645810559378148 1102 36-37 0.49150866952211913 1102 38-39 0.41039620123689957 1102 40-41 0.47953187078488924 1102 42-43 0.5437797873259598 1102 44-45 0.5590548755093394 1102 46-47 0.559027570216827 1102 48-49 0.6533087257155543 1102 50-51 0.738734631950706 1102 52-53 0.7725875666672408 1102 54-55 0.7711000354089776 1102 56-57 0.8589680788901433 1102 58-59 0.9016116226148227 1102 60-61 1.0203846358372672 1102 62-63 1.0755518202997152 1102 64-65 1.2341227288332384 1102 66-67 1.226744600421533 1102 68-69 1.39832565801796 1102 70-71 1.6041409590249174 1102 72-73 1.7399705043542575 1102 74-75 1.640000954440577 1102 76-77 1.697874825831736 1102 78-79 1.7533079670595164 1102 80-81 1.900942107167161 1102 82-83 2.0708609970480794 1102 84-85 2.249646500877521 1102 86-87 2.181890466039203 1102 88-89 1.9622167188181976 1102 90-91 2.172213597933723 1102 92-93 2.4548773364724568 1102 94-95 2.3548088486166634 1102 96-97 2.5158998216082757 1102 98-99 2.5553097015832726 1102 100-101 2.3978460274810818 1103 1 0.3517859385205391 1103 2 0.3169120591018526 1103 3 0.28298727777575294 1103 4 0.2250792708799665 1103 5 0.22784356708206133 1103 6 0.22727481284638174 1103 7 0.20345706925722595 1103 8 0.22192200238978899 1103 9 0.343504101441404 1103 10-11 0.3764847701417722 1103 12-13 0.3439411183218155 1103 14-15 0.4867072194195785 1103 16-17 0.38078744224706895 1103 18-19 0.46921057378632014 1103 20-21 0.5026942608411176 1103 22-23 0.4696534579785663 1103 24-25 0.3627688765873458 1103 26-27 0.44736715470024535 1103 28-29 0.4876746234582967 1103 30-31 0.3745181662543473 1103 32-33 0.5418828723400892 1103 34-35 0.5812625282053645 1103 36-37 0.5591388888897271 1103 38-39 0.5080444705023552 1103 40-41 0.6494324337822803 1103 42-43 0.6915196695244248 1103 44-45 0.7205495454930215 1103 46-47 0.8677602702130542 1103 48-49 0.9965436323930703 1103 50-51 0.9360944279795191 1103 52-53 0.9362729237007201 1103 54-55 1.0214159872815074 1103 56-57 1.1459305149656132 1103 58-59 1.1253383234584788 1103 60-61 1.091936067033707 1103 62-63 1.152350936788153 1103 64-65 1.4494204629994663 1103 66-67 1.451901344014395 1103 68-69 1.4414034015814536 1103 70-71 1.6010087091748986 1103 72-73 1.7280484996304502 1103 74-75 1.4580575121855688 1103 76-77 1.6083212173204906 1103 78-79 1.6191201914865587 1103 80-81 1.805082571241467 1103 82-83 2.195336857800619 1103 84-85 2.131823459097408 1103 86-87 2.074128066042558 1103 88-89 1.9005922070359453 1103 90-91 2.3529747771237943 1103 92-93 2.607994431120627 1103 94-95 2.4372292864289413 1103 96-97 2.441160554008313 1103 98-99 2.355389253282965 1103 100-101 2.1157392866151064 1104 1 0.23473575480587883 1104 2 0.16456334280742624 1104 3 0.16153735512666856 1104 4 0.1816663827896079 1104 5 0.12733444426838503 1104 6 0.1255327694040318 1104 7 0.18470134533956184 1104 8 0.1695007789769818 1104 9 0.27346282431739866 1104 10-11 0.23373020124453348 1104 12-13 0.21409882130429736 1104 14-15 0.3008076692433548 1104 16-17 0.26801434208837804 1104 18-19 0.32555141367042495 1104 20-21 0.3818064306515936 1104 22-23 0.3390133704307132 1104 24-25 0.29778743032000676 1104 26-27 0.5359021164142916 1104 28-29 0.3280567189939134 1104 30-31 0.3417312288212031 1104 32-33 0.4954810006377457 1104 34-35 0.42582033715211054 1104 36-37 0.4383561740474491 1104 38-39 0.43199114481256373 1104 40-41 0.428277920214164 1104 42-43 0.5033713749897188 1104 44-45 0.4997563815360522 1104 46-47 0.5990385652171497 1104 48-49 0.5473962402024668 1104 50-51 0.5865963225525661 1104 52-53 0.6877020305148918 1104 54-55 0.764990780471436 1104 56-57 0.9300775226487659 1104 58-59 0.8293237056838549 1104 60-61 0.8492896470976419 1104 62-63 0.9041330289281291 1104 64-65 1.0767725749440622 1104 66-67 1.1267698998943132 1104 68-69 1.0747600092670098 1104 70-71 1.2525748030086987 1104 72-73 1.475986453611398 1104 74-75 1.4532715804641043 1104 76-77 1.5253692288177625 1104 78-79 1.6042876294984616 1104 80-81 1.7150489043687323 1104 82-83 1.7107326982290267 1104 84-85 2.0198987097013834 1104 86-87 1.9434766804674801 1104 88-89 2.0151641903253505 1104 90-91 2.319081694163067 1104 92-93 2.520971220353619 1104 94-95 2.312904858521094 1104 96-97 2.5270328016590717 1104 98-99 2.671837695328705 1104 100-101 2.3576812964005693 1105 1 0.19882125618695667 1105 2 0.16308417923171703 1105 3 0.18017680385842283 1105 4 0.1659811202138215 1105 5 0.10200468496906723 1105 6 0.14519883006559553 1105 7 0.15708220712567567 1105 8 0.17650687002635124 1105 9 0.3112920009195008 1105 10-11 0.23014202868475309 1105 12-13 0.28571467559046937 1105 14-15 0.37649282060383626 1105 16-17 0.2532952329087763 1105 18-19 0.29276242010506337 1105 20-21 0.2891320172472618 1105 22-23 0.31788400029032005 1105 24-25 0.31274730880664237 1105 26-27 0.4068082987311996 1105 28-29 0.38978340288239366 1105 30-31 0.40807843543581157 1105 32-33 0.4499772580060508 1105 34-35 0.5218846285911027 1105 36-37 0.4947969955546867 1105 38-39 0.4951816114365002 1105 40-41 0.5650859983261185 1105 42-43 0.6397223463607702 1105 44-45 0.7602945642353518 1105 46-47 0.8598087619759553 1105 48-49 0.9783129004210167 1105 50-51 1.0715594215505178 1105 52-53 1.0235778167420406 1105 54-55 1.0644004283282769 1105 56-57 1.2793098530803277 1105 58-59 1.215110259374697 1105 60-61 1.2963470793917722 1105 62-63 1.448252377622893 1105 64-65 1.6967885615527898 1105 66-67 1.6373717356094275 1105 68-69 1.6801000122059335 1105 70-71 1.5919069598260656 1105 72-73 1.6950097962844488 1105 74-75 1.7633997809507527 1105 76-77 1.8046964684988147 1105 78-79 1.9408753711580857 1105 80-81 2.0263611929265473 1105 82-83 2.278107167501304 1105 84-85 2.4350399519574246 1105 86-87 2.537747329304679 1105 88-89 2.475580000693313 1105 90-91 2.616729525926015 1105 92-93 2.737886086456303 1105 94-95 2.419451429220782 1105 96-97 2.4682260519784585 1105 98-99 2.560847895665333 1105 100-101 2.3587003362244054 1106 1 0.08758181198342285 1106 2 0.08659674274461082 1106 3 0.07279420294853622 1106 4 0.09581705708763621 1106 5 0.1830932738508082 1106 6 0.08129203277319164 1106 7 0.1427957882149542 1106 8 0.10440650715425903 1106 9 -0.026224603030648552 1106 10-11 0.12922751839082025 1106 12-13 0.08960806641337626 1106 14-15 0.1313724466750017 1106 16-17 0.04929391212834133 1106 18-19 0.19862324629158934 1106 20-21 0.1570238310802452 1106 22-23 0.25379532166675034 1106 24-25 0.17982082791240117 1106 26-27 -0.009197635190048459 1106 28-29 0.2361541338946367 1106 30-31 0.2958561127392869 1106 32-33 0.1716984787962943 1106 34-35 0.08652015004933133 1106 36-37 0.14099841594170215 1106 38-39 0.16535351840916235 1106 40-41 0.2747153645912732 1106 42-43 0.3195339638852204 1106 44-45 0.24397045786868432 1106 46-47 -0.12038946469819223 1106 48-49 0.07923460376194669 1106 50-51 0.43885729521732486 1106 52-53 0.43099542381396105 1106 54-55 0.17568628481123483 1106 56-57 0.41981283887987786 1106 58-59 0.59423093335635 1106 60-61 0.5671497138281083 1106 62-63 0.5360517368251649 1106 64-65 0.7747512577260238 1106 66-67 0.5838063134769058 1106 68-69 0.6200310480115512 1106 70-71 0.4541571983954569 1106 72-73 0.6401948391310377 1106 74-75 0.7075637536500565 1106 76-77 0.8054282753990698 1106 78-79 1.0162511393583777 1106 80-81 1.0669349446074925 1106 82-83 1.4363802333765996 1106 84-85 1.6836479261008215 1106 86-87 1.5924561033834017 1106 88-89 1.4163180278665948 1106 90-91 1.60280635588855 1106 92-93 1.6577683876558993 1106 94-95 1.5601284182524147 1106 96-97 1.597122713925117 1106 98-99 1.4736448743115709 1106 100-101 1.444696980839245 1107 1 0.07202000245087703 1107 2 0.08037352600171488 1107 3 0.1185089910195316 1107 4 0.07376010294188262 1107 5 0.053354302764716977 1107 6 0.023081012377510035 1107 7 -0.0012204881423940606 1107 8 0.054435119645027896 1107 9 0.0944985940548122 1107 10-11 0.08557102772373071 1107 12-13 0.19679760996770312 1107 14-15 0.21906683687948458 1107 16-17 0.10681309997043797 1107 18-19 0.11358550401368461 1107 20-21 0.09812043209107202 1107 22-23 0.15171305311123007 1107 24-25 0.14076537865260974 1107 26-27 0.23998800090275552 1107 28-29 0.19473607919453428 1107 30-31 0.03502825072575888 1107 32-33 0.14120793839152412 1107 34-35 0.20494894207802616 1107 36-37 0.14046215015568464 1107 38-39 0.2634406860346772 1107 40-41 0.21349348018573266 1107 42-43 0.2780786692639339 1107 44-45 0.23395100792743762 1107 46-47 0.34714245679437283 1107 48-49 0.33626612336914263 1107 50-51 0.3848273569416705 1107 52-53 0.2643587347378187 1107 54-55 0.28396250237099707 1107 56-57 0.17132356747184474 1107 58-59 0.29834561702451623 1107 60-61 0.4652791995458898 1107 62-63 0.600569408860494 1107 64-65 0.687546556104067 1107 66-67 0.5843414325791265 1107 68-69 0.8282554463847873 1107 70-71 0.7531921274757956 1107 72-73 0.9723210966609237 1107 74-75 0.7949946796026772 1107 76-77 1.0258308924591049 1107 78-79 1.2369173298782492 1107 80-81 1.345906074385887 1107 82-83 1.4055078472696003 1107 84-85 1.7642961082247872 1107 86-87 1.8609615048100956 1107 88-89 1.8375340259620074 1107 90-91 2.210581854859811 1107 92-93 2.3280322013732935 1107 94-95 2.274089238797494 1107 96-97 2.2287252099827093 1107 98-99 2.340981204311877 1107 100-101 2.061442222647555 1108 1 0.04554812715274892 1108 2 0.09510835201887602 1108 3 0.0886669630379302 1108 4 0.09782651880006199 1108 5 0.09901344241139753 1108 6 0.02652821193618138 1108 7 0.09220408472963726 1108 8 0.07771509741238702 1108 9 0.047729066871674775 1108 10-11 0.17572815979639245 1108 12-13 0.16159014336851385 1108 14-15 0.2526247952098757 1108 16-17 0.11333584901953486 1108 18-19 0.0836999232897142 1108 20-21 0.0604787301328642 1108 22-23 0.15411436467095285 1108 24-25 0.2323123616919318 1108 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2316 58-59 -0.5222300309992605 2316 60-61 -0.455144827857076 2316 62-63 -0.42121027326184546 2316 64-65 -0.44923438144314076 2316 66-67 -0.36448826648634736 2316 68-69 -0.08956806537538142 2316 70-71 -0.4906149535287092 2316 72-73 -0.36995048746070935 2316 74-75 -0.044046277868019956 2316 76-77 -0.027964114011947316 2316 78-79 -0.14883951780094762 2316 80-81 -0.6249474512145738 2316 82-83 -0.11949353063574009 2316 84-85 0.13337851512941867 2316 86-87 0.23151423877842348 2316 88-89 0.22276905578599937 2316 90-91 0.40004373689478356 2316 92-93 0.3265650383885266 2316 94-95 0.820759913297465 2316 96-97 0.7235533698768855 2316 98-99 0.7483288079813946 2316 100-101 0.2632433490596533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 19.0 4 52.0 5 130.0 6 261.0 7 659.0 8 1499.0 9 2732.0 10 4608.0 11 7120.0 12 10291.0 13 14194.0 14 18833.0 15 25538.0 16 32615.0 17 41207.0 18 39544.0 19 33796.0 20 34067.0 21 36962.0 22 40224.0 23 45478.0 24 51249.0 25 58815.0 26 68250.0 27 80368.0 28 94547.0 29 110959.0 30 129195.0 31 151045.0 32 178425.0 33 213306.0 34 259999.0 35 317064.0 36 363869.0 37 323671.0 38 104867.0 39 1966.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.17251988085983 19.821483065884735 15.781929855327823 22.224067197927614 2 15.812703802870526 21.311269437965745 39.352407803524976 23.52361895563876 3 18.8256542435404 25.46600529027484 30.246442317780957 25.461898148403804 4 13.167966265311398 17.887971870272757 36.8130341581669 32.13102770624894 5 13.720877579532717 37.83609164554446 33.60384948837312 14.839181286549707 6 31.211593158495816 36.81275805169611 17.451447539943114 14.524201249864966 7 28.274234468924735 33.43072989090688 20.361092042507973 17.933943597660413 8 26.577155218828256 34.62718020647249 19.775718636364893 19.01994593833436 9 26.85072888833054 16.71904649161014 20.036094105595794 36.39413051446353 10-11 24.85367219880494 27.11924658827314 27.018070823380558 21.009010389541363 12-13 26.013043269680438 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29.394444915173075 21.864537493017444 38-39 21.962927320261258 26.579477305660276 29.34285929281556 22.114736081262905 40-41 22.156163393429456 26.402920732972255 29.456960299004415 21.983955574593875 42-43 22.291846720105145 26.361083192281885 29.080868039352666 22.266202048260304 44-45 22.087993308514644 26.72770041279921 29.009442387149782 22.174863891536365 46-47 22.304885445993996 26.94178380341906 28.40488950130848 22.348441249278455 48-49 22.324398873071438 26.85666933454544 28.390665116328833 22.428266676054285 50-51 22.49969196871852 27.0971580706094 28.04641211720858 22.356737843463506 52-53 22.62708059168236 27.106286840800113 27.8457172262482 22.420915341269325 54-55 22.263327573062085 27.12375057507801 28.029051922857235 22.583869929002674 56-57 22.369197147958207 27.268810012172846 27.854690686549095 22.507302153319852 58-59 22.295045303894863 26.88027643779856 28.028240860099267 22.79643739820731 60-61 22.30357009118071 27.025922601143844 28.107190054092708 22.563317253582742 62-63 22.076213669134024 27.164890439226724 28.08941570003517 22.669480191604087 64-65 21.925526440491595 26.917569391643838 28.05283369992245 23.104070467942115 66-67 22.011624828856302 27.09166330447892 27.94564748833358 22.9510643783312 68-69 22.265651896378294 26.856674654850575 27.95186174384695 22.925811704924183 70-71 22.164502538022887 26.879297446963818 27.820751648226487 23.1354483667868 72-73 22.253058015449227 26.936490019677805 27.64739675018503 23.16305521468793 74-75 22.184447405686345 27.193795335388256 27.339924685057426 23.281832573867973 76-77 22.10715485051768 26.971029183418565 27.79660478775523 23.125211178308525 78-79 22.17706155759253 27.01777746025534 27.694255570361765 23.110905411790366 80-81 22.055605158918475 27.278787504370257 27.582004597869354 23.08360273884192 82-83 22.279033712650552 27.185917438364548 27.409480019838483 23.125568829146417 84-85 22.19432237542402 27.007701738327277 27.38460853466093 23.413367351587773 86-87 22.293993789755636 26.97958226416612 27.28452034551366 23.441903600564594 88-89 22.132729212375228 27.037777922733675 27.179178949090456 23.65031391580064 90-91 21.95180498344202 27.01040072202789 27.516061841757853 23.52173245277223 92-93 22.02224121233624 26.719360781717764 27.494017078677746 23.76438092726825 94-95 21.780432389934393 26.74772928156011 27.468678287765957 24.003160040739537 96-97 21.978600360595173 26.660297808542182 27.362126186222834 23.998975644639806 98-99 21.836156349430684 26.683964737062084 27.11853906544172 24.361339848065512 100-101 22.009453205081744 26.451683043248668 27.27341126446541 24.26545248720418 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1329.0 1 931.0 2 613.0 3 723.5 4 671.5 5 658.5 6 1063.0 7 2348.5 8 3735.5 9 4072.5 10 3451.0 11 2843.0 12 2852.5 13 3099.5 14 3480.5 15 3843.0 16 4173.0 17 4532.0 18 4889.5 19 5252.5 20 5764.5 21 6489.5 22 7575.0 23 9127.0 24 10782.0 25 13107.5 26 16458.5 27 20619.0 28 24473.5 29 28441.5 30 33320.5 31 39056.0 32 46109.5 33 50674.0 34 54392.0 35 59350.5 36 65206.0 37 71225.5 38 74911.0 39 78378.0 40 83094.5 41 89546.0 42 95603.0 43 99801.5 44 102490.5 45 106308.0 46 108406.0 47 109663.0 48 112175.0 49 111946.5 50 109352.0 51 104649.0 52 100285.0 53 98101.0 54 96335.0 55 89931.0 56 79962.0 57 72271.0 58 66171.0 59 57935.5 60 48252.5 61 39808.0 62 32973.5 63 25631.5 64 18961.5 65 14803.0 66 11680.0 67 9243.5 68 7114.5 69 5250.0 70 4100.5 71 3369.0 72 2904.5 73 2617.0 74 2169.0 75 1798.0 76 1293.5 77 757.5 78 500.5 79 337.5 80 207.5 81 130.0 82 73.5 83 30.0 84 16.0 85 10.0 86 6.0 87 5.5 88 2.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023365510091173807 2 0.011458418538064556 3 0.0014150456628332735 4 0.0 5 3.106197796463283E-4 6 0.0 7 0.0 8 3.451330884959204E-5 9 2.4159316194714424E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002001771913276338 26-27 0.009042486918593114 28-29 2.588498163719403E-4 30-31 0.0 32-33 3.969030517703084E-4 34-35 0.009991602911956894 36-37 0.0011561958464613332 38-39 0.012666384347800277 40-41 0.012148684715056396 42-43 0.005004429783190845 44-45 6.730095225670447E-4 46-47 1.725665442479602E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.902661769918408E-5 66-67 4.831863238942885E-4 68-69 9.83629302213373E-4 70-71 1.725665442479602E-5 72-73 2.2433650752234826E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3805323539836816E-4 82-83 0.0011734525008861292 84-85 0.005694695960182686 86-87 0.004262393642924617 88-89 0.0 90-91 0.0013115057362844975 92-93 6.557528681422487E-4 94-95 6.902661769918408E-5 96-97 3.451330884959204E-5 98-99 0.0 100-101 1.035399265487761E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2897433.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.03940123125037 #Duplication Level Percentage of deduplicated Percentage of total 1 68.12191646825431 30.681703284554278 2 15.586212896016063 14.039873925987125 3 6.565681407453628 8.871430778005939 4 3.218568825485542 5.798496508857503 5 1.812154098110596 4.080916775882889 6 1.1353707331871477 3.068185079894091 7 0.7692592143338874 2.4252882083654272 8 0.5193089930775229 1.871149288177214 9 0.3737468172612784 1.5149999575380177 >10 1.6944019347167734 13.351593949659996 >50 0.11794358230371016 3.693535194508551 >100 0.07761232926718767 6.509549846054787 >500 0.005445213115786323 1.5965718903883221 >1k 0.0021474079891950623 1.4017888070931797 >5k 7.669314247125224E-5 0.266354381043209 >10k+ 1.5338628494250447E-4 0.8285621239895544 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13314 0.4595101940234683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10673 0.36836054535169577 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7711 0.26613212453920415 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.902661769918407E-5 0.0 0.0 0.0 0.0 3 6.902661769918407E-5 0.0 0.0 0.0 0.0 4 6.902661769918407E-5 0.0 0.0 0.0 0.0 5 6.902661769918407E-5 0.0 0.0 0.0 0.0 6 6.902661769918407E-5 0.0 0.0 0.0 0.0 7 1.7256654424796018E-4 0.0 0.0 0.0 0.0 8 2.0707985309755222E-4 0.0 0.0 0.0 0.0 9 2.0707985309755222E-4 0.0 0.0 0.0 0.0 10-11 2.7610647079673626E-4 0.0 0.0 0.0 0.0 12-13 3.7964639734551237E-4 0.0 0.0 1.7256654424796016E-5 0.0 14-15 5.522129415934725E-4 0.0 0.0 3.451330884959203E-5 0.0 16-17 5.694695960182686E-4 0.0 0.0 1.0353992654877611E-4 0.0 18-19 7.938061035406168E-4 0.0 0.0 1.2079658097357212E-4 0.0 20-21 9.31859338938985E-4 0.0 0.0 2.2433650752234823E-4 0.0 22-23 0.001000885956638169 0.0 0.0 7.075228314166367E-4 0.0 24-25 0.0011734525008861292 0.0 0.0 0.0013115057362844975 0.0 26-27 0.0012252224641605173 3.451330884959203E-5 0.0 0.0024849582371706267 0.0 28-29 0.0014495589716828653 3.451330884959203E-5 0.0 0.006384962137174526 0.0 30-31 0.0016566388247804178 3.451330884959203E-5 0.0 0.018688956742054088 0.0 32-33 0.0016911521336300099 3.451330884959203E-5 0.0 0.04241685657614861 0.0 34-35 0.002001771913276338 3.451330884959203E-5 0.0 0.07510096005671227 0.0 36-37 0.0024159316194714425 3.451330884959203E-5 0.0 0.12678464005897633 0.0 38-39 0.0025884981637194025 3.451330884959203E-5 0.0 0.20278294614577802 0.0 40-41 0.0026057548181441983 3.451330884959203E-5 0.0 0.2891007315786077 0.0 42-43 0.002968144561064915 3.451330884959203E-5 0.0 0.37735126230701455 0.0 44-45 0.0034168175761096113 3.451330884959203E-5 0.0 0.4880009304788066 0.0 46-47 0.003692924046906348 3.451330884959203E-5 0.0 0.6219643387784981 0.0 48-49 0.003830977282304716 3.451330884959203E-5 0.0 0.7631927985910287 0.0 50-51 0.003830977282304716 3.451330884959203E-5 0.0 0.917639855692953 0.0 52-53 0.003848233936729512 3.451330884959203E-5 0.0 1.071690009743107 0.0 54-55 0.003865490591154308 3.451330884959203E-5 0.0 1.239718053877346 0.0 56-57 0.00407257044425186 3.451330884959203E-5 0.0 1.4309735548673603 0.0 58-59 0.00407257044425186 3.451330884959203E-5 0.0 1.6381914612003108 0.0 60-61 0.004089827098676656 3.451330884959203E-5 0.0 1.8690854974040816 0.0 62-63 0.004107083753101452 3.451330884959203E-5 0.0 2.1043972371406 0.0 64-65 0.004107083753101452 3.451330884959203E-5 0.0 2.350839518981112 0.0 66-67 0.004348676915048597 3.451330884959203E-5 0.0 2.628205725550858 0.0 68-69 0.00441770353274778 3.451330884959203E-5 0.0 2.9353741743122272 0.0 70-71 0.004573013422570944 3.451330884959203E-5 0.0 3.267478488717427 0.0 72-73 0.004780093275668498 3.451330884959203E-5 0.0 3.6107996284987434 0.0 74-75 0.004831863238942885 3.451330884959203E-5 0.0 3.963525644941574 0.0 76-77 0.004831863238942885 3.451330884959203E-5 0.0 4.345070274273814 0.0 78-79 0.004831863238942885 3.451330884959203E-5 0.0 4.769549459814947 0.0 80-81 0.0053668195261115615 3.451330884959203E-5 0.0 5.2176875185724745 0.0 82-83 0.0054531027982355415 3.451330884959203E-5 0.0 5.678854351420723 0.0 84-85 0.00559115603363391 3.451330884959203E-5 0.0 6.147803935414555 0.0 86-87 0.005729209269032278 3.451330884959203E-5 0.0 6.63846239067478 3.451330884959203E-5 88-89 0.00576372257788187 3.451330884959203E-5 0.0 7.15619653672751 3.451330884959203E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6790 0.0 54.02281 1 GTATCAA 12865 0.0 45.021873 1 TATCAAC 17325 0.0 33.288376 2 ATCAACG 17360 0.0 33.0793 3 TCAACGC 17660 0.0 32.54426 4 CAACGCA 18040 0.0 31.858736 5 AACGCAG 18695 0.0 30.843628 6 ACGCAGA 21385 0.0 26.897205 7 CGCAGAG 21515 0.0 26.6243 8 GTACATG 15875 0.0 24.543118 1 GCAGAGT 22715 0.0 24.506826 9 TACATGG 15875 0.0 24.23921 2 ACATGGG 16170 0.0 22.823946 3 GAGTACT 12455 0.0 22.518963 12-13 TGGTATC 3150 0.0 22.471027 2 AGAGTAC 21345 0.0 21.49578 10-11 AGTACTT 12960 0.0 21.458242 12-13 GTACTTT 13165 0.0 21.124104 14-15 CAGAGTA 22260 0.0 20.37748 10-11 GTATAGG 635 0.0 20.203178 1 >>END_MODULE