FastQCFastQC Report
Thu 26 May 2016
SRR938671_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938671_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019116
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74810.7340675644381993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62100.6093516341613713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.3976976124405857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32500.31890383430345515No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16140.15837255032793127No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11030.10823105514975724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31100.051.6791041
GTATCAA56350.041.3486061
TATCAAC72050.032.0101552
ATCAACG73100.031.3552113
TCAACGC73900.030.887084
CAACGCA75550.030.2754545
AACGCAG76550.029.8564746
GTGGTAT14750.029.0141071
CATTCCG2253.6379788E-1227.4522619
ACGCAGA85100.026.7451217
CGCAGAG85000.026.7206888
CCGTATC1351.8189894E-1224.6336294-95
GCAGAGT89250.024.3290569
GACGTGA2151.6552804E-924.3104447
GTATAGT3600.023.7754481
CGCCGTA1800.023.74976592-93
GCCGTAT1750.023.07516994-95
TGGTATC18050.022.9203932
TAGGACG4000.022.5877844
GAGTACT60550.022.47831512-13
Produced by FastQC (version 0.11.5)