##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938671_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1019116 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16774930429902 31.0 31.0 34.0 28.0 34.0 2 31.20760639613155 31.0 31.0 34.0 28.0 34.0 3 31.222108180030535 31.0 31.0 34.0 27.0 34.0 4 34.88876241762468 37.0 35.0 37.0 32.0 37.0 5 34.890074338936884 37.0 35.0 37.0 32.0 37.0 6 34.919473347489394 37.0 35.0 37.0 32.0 37.0 7 34.85336311077444 37.0 35.0 37.0 32.0 37.0 8 34.840556914031374 37.0 35.0 37.0 32.0 37.0 9 36.34919675483458 39.0 37.0 39.0 32.0 39.0 10-11 36.205494762127174 39.0 35.0 39.0 32.0 39.0 12-13 36.102932345287485 39.0 35.0 39.0 32.0 39.0 14-15 37.05541616459755 39.0 36.0 41.0 31.0 41.0 16-17 36.91028940768274 39.0 36.0 41.0 31.0 41.0 18-19 37.11193426459795 39.0 36.5 41.0 31.0 41.0 20-21 37.14606139045997 40.0 36.0 41.0 31.0 41.0 22-23 37.04928879538737 39.0 36.0 41.0 31.0 41.0 24-25 36.94354077455363 39.0 36.0 41.0 31.0 41.0 26-27 36.758648181365025 39.0 36.0 41.0 30.0 41.0 28-29 36.566357509841865 39.0 36.0 41.0 30.0 41.0 30-31 36.287684620788994 39.0 35.0 40.0 30.0 41.0 32-33 35.97729012202733 38.0 35.0 40.0 29.5 41.0 34-35 35.72534971485091 38.0 35.0 40.0 28.5 41.0 36-37 35.452598134069135 38.0 35.0 40.0 27.0 41.0 38-39 35.26035309032534 38.0 35.0 40.0 26.5 41.0 40-41 35.13567003167451 38.0 34.5 40.0 25.5 41.0 42-43 34.8055221387948 38.0 34.0 40.0 24.5 41.0 44-45 34.35769676857198 38.0 33.5 40.0 23.5 41.0 46-47 33.84299235808289 38.0 33.0 40.0 20.0 41.0 48-49 33.41612632909306 38.0 33.0 40.0 15.5 41.0 50-51 32.71910361529011 37.5 32.0 39.5 12.5 40.5 52-53 32.75183394235789 37.5 32.5 39.5 9.0 40.5 54-55 32.87399275450488 38.0 32.5 40.0 8.0 41.0 56-57 32.612408204757855 38.0 32.0 40.0 4.5 41.0 58-59 32.29540013109401 37.5 31.0 40.0 2.0 41.0 60-61 31.906044061716234 37.0 31.0 40.0 2.0 41.0 62-63 31.40131986937699 36.5 30.5 39.0 2.0 41.0 64-65 30.971455653723424 36.0 29.5 39.0 2.0 40.0 66-67 30.506225984088168 35.5 29.0 39.0 2.0 40.0 68-69 29.975331071242138 35.0 28.0 38.5 2.0 40.0 70-71 29.413790481162103 35.0 28.0 38.0 2.0 40.0 72-73 28.797686916896605 34.0 26.0 37.0 2.0 39.0 74-75 28.151293866448963 34.0 26.0 36.5 2.0 39.0 76-77 27.435500472958918 33.5 25.5 36.0 2.0 39.0 78-79 26.668566679357404 33.0 22.5 35.5 2.0 37.5 80-81 25.97266797891506 32.0 20.5 35.0 2.0 37.0 82-83 25.25525651643189 31.5 18.5 35.0 2.0 36.5 84-85 24.54230234830971 31.0 11.0 34.5 2.0 36.0 86-87 23.803537575702865 31.0 4.5 34.0 2.0 35.5 88-89 23.005254063325474 30.0 2.0 34.0 2.0 35.0 90-91 22.104686316376153 29.0 2.0 34.0 2.0 35.0 92-93 21.18303755411553 29.0 2.0 34.0 2.0 35.0 94-95 20.193148768148085 28.0 2.0 34.0 2.0 35.0 96-97 18.95698625082915 25.5 2.0 33.0 2.0 35.0 98-99 17.46617215312094 22.5 2.0 32.5 2.0 34.0 100-101 13.867161343752821 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4118564246337293 1101 2 0.2113995930108601 1101 3 0.34239304104548296 1101 4 0.19036921397647433 1101 5 0.07952224771006655 1101 6 0.20622172829758512 1101 7 0.2635939752312524 1101 8 0.4347674637205685 1101 9 0.24583803677888483 1101 10-11 0.3675194287413177 1101 12-13 0.5405546686204801 1101 14-15 0.5792283772417122 1101 16-17 0.5335301374570705 1101 18-19 0.5564343628346506 1101 20-21 0.6779048870954512 1101 22-23 0.7525442587134137 1101 24-25 0.8110514449765844 1101 26-27 0.6829802706218473 1101 28-29 0.5824702791283087 1101 30-31 0.8110562783605886 1101 32-33 0.659430533632694 1101 34-35 0.6476161516497072 1101 36-37 0.7165311373137442 1101 38-39 1.048754827369585 1101 40-41 0.9459310267593182 1101 42-43 1.2158263645966798 1101 44-45 1.1939823089798622 1101 46-47 1.0010789419036783 1101 48-49 0.6209092032783161 1101 50-51 0.9896277730305982 1101 52-53 0.7768752836899395 1101 54-55 0.7671354188820629 1101 56-57 0.9377271828876559 1101 58-59 0.8346178796710362 1101 60-61 1.0749629233019355 1101 62-63 1.0273457262940262 1101 64-65 0.9828898002149167 1101 66-67 1.0310030592451547 1101 68-69 0.9715264865456739 1101 70-71 0.9467462691204638 1101 72-73 1.1275635728456308 1101 74-75 1.0995199503118478 1101 76-77 0.9929064965813374 1101 78-79 1.1809630274222975 1101 80-81 1.264487792631396 1101 82-83 1.458419072597927 1101 84-85 1.2995071445269524 1101 86-87 1.2403843125418454 1101 88-89 1.2342998136331182 1101 90-91 1.120813077570599 1101 92-93 1.0142717930852072 1101 94-95 1.2622494596329759 1101 96-97 1.5803532148198904 1101 98-99 1.818909403581678 1101 100-101 2.003362551848003 1102 1 0.5788989542107927 1102 2 0.40523977059132577 1102 3 0.4271936172374211 1102 4 0.3736891658030501 1102 5 0.40385218843786674 1102 6 0.591683508352844 1102 7 0.5259230658397982 1102 8 0.5398669985164233 1102 9 0.5163991154857612 1102 10-11 0.74129265943062 1102 12-13 0.6473898635575495 1102 14-15 0.7580562753218558 1102 16-17 0.8184983760571996 1102 18-19 0.8919722994063051 1102 20-21 0.8449946455198969 1102 22-23 0.9628318823939566 1102 24-25 0.8112716494774972 1102 26-27 0.8438718331882598 1102 28-29 0.7593570715811424 1102 30-31 0.8983877304114927 1102 32-33 0.9056457318757865 1102 34-35 0.7610149756513991 1102 36-37 0.9516085926234368 1102 38-39 0.8301141916692956 1102 40-41 1.0501178640795459 1102 42-43 1.1197735155164636 1102 44-45 1.1478190624688906 1102 46-47 1.069932697777766 1102 48-49 1.1796483111253906 1102 50-51 1.1529291875345749 1102 52-53 1.0772855843015563 1102 54-55 0.8875010882329022 1102 56-57 0.9259471276299038 1102 58-59 0.9067357392596733 1102 60-61 0.9312608901000523 1102 62-63 0.9219297582207311 1102 64-65 1.1247522698113421 1102 66-67 1.148367925705589 1102 68-69 1.1544384992030068 1102 70-71 1.1901649292380618 1102 72-73 1.0398413020826496 1102 74-75 0.9632452437210617 1102 76-77 1.2598817250508887 1102 78-79 1.2972079798392322 1102 80-81 1.3199326883737186 1102 82-83 1.4435313591825754 1102 84-85 1.4087935166430405 1102 86-87 1.6968853633836964 1102 88-89 1.3206211587106935 1102 90-91 1.306991555384851 1102 92-93 1.7402033820997787 1102 94-95 1.7497739595975546 1102 96-97 1.7138183953521597 1102 98-99 1.9480053253707084 1102 100-101 1.9707291893902124 1103 1 0.40585918959273926 1103 2 0.3868187179597484 1103 3 0.4253612829599227 1103 4 0.432458899900503 1103 5 0.34451046742695013 1103 6 0.40062560702882166 1103 7 0.34467347753863464 1103 8 0.46646713465150214 1103 9 0.6916389986730778 1103 10-11 0.818898987516171 1103 12-13 0.6327037427486459 1103 14-15 0.7557963231887115 1103 16-17 0.832058088856094 1103 18-19 0.8597231063360198 1103 20-21 0.669473709261851 1103 22-23 0.6936039000475986 1103 24-25 0.94321453861523 1103 26-27 0.787123814831979 1103 28-29 0.8435933976905332 1103 30-31 1.0677106779555814 1103 32-33 0.9402004384168592 1103 34-35 1.0102541562060452 1103 36-37 1.1222401935280786 1103 38-39 1.0819635428319359 1103 40-41 1.1839803676476066 1103 42-43 1.244478739151269 1103 44-45 1.1370572549463347 1103 46-47 0.6646398975669783 1103 48-49 0.6033945509427028 1103 50-51 0.7359622551849299 1103 52-53 0.867895008362332 1103 54-55 1.0404087798649826 1103 56-57 1.035396439269448 1103 58-59 0.8395733871967863 1103 60-61 1.0685081070159335 1103 62-63 1.106380716655618 1103 64-65 1.15612811242163 1103 66-67 1.2056335867292383 1103 68-69 1.3368710354873983 1103 70-71 1.2531504593963767 1103 72-73 1.4783823512010628 1103 74-75 1.4910634594732066 1103 76-77 1.4923126145399408 1103 78-79 1.5528208072770404 1103 80-81 1.5712814137541287 1103 82-83 1.6744460990986987 1103 84-85 1.7359228230128458 1103 86-87 2.183731860101087 1103 88-89 2.013920897419169 1103 90-91 2.204881461627302 1103 92-93 2.1846575243389807 1103 94-95 2.2645665731715354 1103 96-97 2.4345405700002942 1103 98-99 2.236953681868961 1103 100-101 2.0127186279427924 1104 1 0.5269840605937723 1104 2 0.39723977059132665 1104 3 0.5102787236203987 1104 4 0.4830295913349687 1104 5 0.5721926139697828 1104 6 0.5393005296294362 1104 7 0.5968592360525662 1104 8 0.5267818921334424 1104 9 0.5732076261240593 1104 10-11 0.6072075530476368 1104 12-13 0.5698260337703189 1104 14-15 0.6939392540452687 1104 16-17 0.7992005037167829 1104 18-19 0.8538871930233327 1104 20-21 0.8402606029667012 1104 22-23 0.9689382653726781 1104 24-25 0.6743461175625995 1104 26-27 0.8200845991457086 1104 28-29 0.9813570715811437 1104 30-31 1.0750579431774483 1104 32-33 0.9907840297481201 1104 34-35 0.9514724224599149 1104 36-37 1.1863851883681278 1104 38-39 1.292890787413981 1104 40-41 1.286575310888061 1104 42-43 1.2524756431760338 1104 44-45 1.235329700766762 1104 46-47 0.9925284424586138 1104 48-49 0.8515312898487934 1104 50-51 0.8739823790239356 1104 52-53 0.9772004779185721 1104 54-55 1.0311925775946023 1104 56-57 1.0344258510341575 1104 58-59 1.1015868030894609 1104 60-61 0.9694523794617602 1104 62-63 1.0586531624760518 1104 64-65 0.946741631513472 1104 66-67 0.9548998405992073 1104 68-69 1.0445980736710894 1104 70-71 1.125888333493382 1104 72-73 1.3614370467634984 1104 74-75 1.1938090735082945 1104 76-77 1.372796618667909 1104 78-79 1.3324952138817885 1104 80-81 1.355826305394995 1104 82-83 1.2905100825868274 1104 84-85 1.4516658570685728 1104 86-87 1.5227045123198693 1104 88-89 1.83392966934899 1104 90-91 1.7716404915550612 1104 92-93 1.8565331693338187 1104 94-95 2.2298271510869156 1104 96-97 2.395552437905348 1104 98-99 2.5564521338813435 1104 100-101 2.1261866361987245 1105 1 0.4325525737133056 1105 2 0.3655410339538143 1105 3 0.24090554577783152 1105 4 0.30691394507646663 1105 5 0.38037142835267446 1105 6 0.35502842078589936 1105 7 0.4426434926123335 1105 8 0.39583437026755064 1105 9 0.5442085979413562 1105 10-11 0.3920365132031307 1105 12-13 0.6412725838189104 1105 14-15 0.6785549003037659 1105 16-17 0.49521508097625144 1105 18-19 0.6069046857347047 1105 20-21 0.7611133726460011 1105 22-23 0.9173746113396319 1105 24-25 0.735884990254533 1105 26-27 0.7818931511379361 1105 28-29 0.6423745642925169 1105 30-31 0.7300146973076735 1105 32-33 0.46553087134190463 1105 34-35 0.7434933165318327 1105 36-37 0.8467675984750187 1105 38-39 0.8391701848872515 1105 40-41 0.9501860980600725 1105 42-43 1.0051393943908096 1105 44-45 1.330600837793007 1105 46-47 1.1271406873565724 1105 48-49 0.9366872665251762 1105 50-51 1.1995883071094582 1105 52-53 1.3693316732538463 1105 54-55 1.0078694483429018 1105 56-57 0.9876916430652614 1105 58-59 1.0406237321467984 1105 60-61 1.2817798818912998 1105 62-63 1.1638698777335854 1105 64-65 1.3044340513385428 1105 66-67 1.2586908998800617 1105 68-69 1.247671445877117 1105 70-71 1.4460020361172887 1105 72-73 1.2638860263553369 1105 74-75 1.298055429193429 1105 76-77 1.4082203310099892 1105 78-79 1.5241040574192048 1105 80-81 1.5793598330917895 1105 82-83 1.6667919096033508 1105 84-85 1.6589010368547754 1105 86-87 1.7154212275774015 1105 88-89 1.6778767053062325 1105 90-91 1.4252658559865488 1105 92-93 1.2868562985855228 1105 94-95 2.0013650519615496 1105 96-97 2.1099445661852343 1105 98-99 2.3454181202759052 1105 100-101 1.6929305623406101 1106 1 0.15094924434619017 1106 2 0.14015272997237105 1106 3 0.09150696346565823 1106 4 0.27204506522278393 1106 5 0.2222274302173659 1106 6 0.34767963988088724 1106 7 0.3956522728030478 1106 8 0.27361941631139075 1106 9 0.09483238434456354 1106 10-11 0.22886519327975208 1106 12-13 0.2559005018554288 1106 14-15 0.5577177840259182 1106 16-17 0.19074208979027674 1106 18-19 0.19801678683377588 1106 20-21 0.2845497712452314 1106 22-23 0.28526901972470853 1106 24-25 0.17234515044460608 1106 26-27 0.3655836320277217 1106 28-29 0.42400890910531785 1106 30-31 0.2528848290575283 1106 32-33 0.47194166998023235 1106 34-35 0.1916726158835118 1106 36-37 0.18260472415148854 1106 38-39 0.30405036188206225 1106 40-41 0.29915074609115067 1106 42-43 -0.20024582684330738 1106 44-45 0.3787184821981029 1106 46-47 0.4359868563851066 1106 48-49 0.593719877856536 1106 50-51 0.28382860726378567 1106 52-53 0.07539970422418207 1106 54-55 0.47236569171452913 1106 56-57 -0.5400103191786059 1106 58-59 -0.2121714174134368 1106 60-61 0.2704291686300344 1106 62-63 0.24746167311434775 1106 64-65 0.3530888268713035 1106 66-67 -0.06836321549363333 1106 68-69 0.05235919552795565 1106 70-71 0.005832240650054388 1106 72-73 0.33727843941340296 1106 74-75 0.7680324777636152 1106 76-77 0.45214478114373335 1106 78-79 0.6606025639784967 1106 80-81 0.5724955510429659 1106 82-83 0.33076540173576774 1106 84-85 0.705311891884822 1106 86-87 0.5746416496506264 1106 88-89 0.5977971741845813 1106 90-91 0.7245931027736319 1106 92-93 0.9393813318096456 1106 94-95 0.8746288918992917 1106 96-97 0.5545340626635706 1106 98-99 0.9256165439393698 1106 100-101 0.4053036574753204 1107 1 0.340682312663688 1107 2 0.12506497758304747 1107 3 0.18778102352840165 1107 4 0.15674716263211508 1107 5 0.19946768296701833 1107 6 0.04082030883827059 1107 7 0.24997423145274666 1107 8 0.14478097217023844 1107 9 0.30501995363096057 1107 10-11 0.32088464596392896 1107 12-13 0.3582855553894504 1107 14-15 0.6474889320581454 1107 16-17 0.6010376702301201 1107 18-19 0.465262538009533 1107 20-21 0.5322316241258562 1107 22-23 0.3202013748483026 1107 24-25 0.35141189493150904 1107 26-27 0.41125571230118396 1107 28-29 0.4142558756289745 1107 30-31 0.3978284123586846 1107 32-33 0.34133002790819233 1107 34-35 0.43902394039919557 1107 36-37 0.4095687210268153 1107 38-39 0.4992978711306293 1107 40-41 0.3726530477785843 1107 42-43 0.558798550259759 1107 44-45 0.8862975020547097 1107 46-47 0.8352588932405851 1107 48-49 0.7364222742094242 1107 50-51 0.29591936154464094 1107 52-53 0.5135473040455238 1107 54-55 0.39595936692303013 1107 56-57 0.5824769089918362 1107 58-59 0.34841936978678945 1107 60-61 0.40473756805421246 1107 62-63 0.4577368791273848 1107 64-65 0.3949444833993958 1107 66-67 0.26934602275192177 1107 68-69 0.5265552953822237 1107 70-71 0.7286399434289841 1107 72-73 0.8291095398637758 1107 74-75 0.6996126613647817 1107 76-77 0.7898646959448179 1107 78-79 0.8876897860988997 1107 80-81 0.9382071701604069 1107 82-83 0.7804659243531589 1107 84-85 0.6832527935911124 1107 86-87 0.9550104000843582 1107 88-89 1.0227654559175292 1107 90-91 0.8054413195219432 1107 92-93 0.8090699678618805 1107 94-95 0.6090704813537045 1107 96-97 1.127225390987224 1107 98-99 1.1452635414250452 1107 100-101 0.742597399768183 1108 1 0.25644352005323157 1108 2 0.19828996364152118 1108 3 0.1950856734273465 1108 4 0.0747940700994505 1108 5 0.15630844408561018 1108 6 0.22062871495762693 1108 7 0.036056147249475146 1108 8 0.20575872611027535 1108 9 0.3033620662784955 1108 10-11 0.5667403715804582 1108 12-13 0.4003800878243666 1108 14-15 0.4010222656282778 1108 16-17 0.5663974149136877 1108 18-19 0.5911651853013211 1108 20-21 0.6451158153219154 1108 22-23 0.509907377341797 1108 24-25 0.6285681252846089 1108 26-27 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2316 72-73 -2.4486530433265905 2316 74-75 -2.6548350706358477 2316 76-77 -2.4933385164672295 2316 78-79 -2.7930498311632554 2316 80-81 -2.8464800009113134 2316 82-83 -2.6671836111068643 2316 84-85 -2.8708116204089045 2316 86-87 -2.889236929121573 2316 88-89 -3.3753631234438046 2316 90-91 -2.950436085021515 2316 92-93 -3.3709668306661804 2316 94-95 -3.7162314074716463 2316 96-97 -3.8854340485811356 2316 98-99 -3.5907190372898263 2316 100-101 -3.1910160665039786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6664.0 3 1246.0 4 1182.0 5 1079.0 6 1290.0 7 1503.0 8 1918.0 9 2392.0 10 3350.0 11 4567.0 12 6156.0 13 8229.0 14 10051.0 15 12255.0 16 15089.0 17 18090.0 18 15670.0 19 12036.0 20 12185.0 21 12814.0 22 13718.0 23 15467.0 24 17164.0 25 19115.0 26 21580.0 27 25001.0 28 28421.0 29 32598.0 30 38310.0 31 44760.0 32 52385.0 33 63510.0 34 77235.0 35 96723.0 36 119048.0 37 134201.0 38 68967.0 39 3147.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.48667876944213 21.260880231588246 15.533290810068202 21.71915018890143 2 15.26257630747924 22.00120815059646 41.06238673588158 21.673828806042717 3 18.000449915468938 26.50797814078818 31.806076609405594 23.685495334337293 4 11.885699241413592 19.278232833568776 39.09578700638344 29.740280918634195 5 12.186238677271401 38.72699951746037 35.41408485031935 13.672676954948882 6 28.515275479184982 38.79819473841877 18.80163239648966 13.884897385906584 7 25.402750105509043 35.055110072923924 22.331406361950005 17.21073345961703 8 24.250273350621 35.7287419589768 21.292786488136485 18.72819820226572 9 25.631545732655862 17.104905441122632 22.308416324054928 34.95513250216658 10-11 22.914782974069592 28.285773469950747 29.1668261976358 19.63261735834386 12-13 24.319716620793283 26.654620301414393 28.80342874702584 20.222234330766486 14-15 21.503603318439126 28.242557190564877 28.130470308508837 22.12336918248716 16-17 19.361107880219137 30.742714175010953 29.364685581862375 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19.34675204149189 28.80492421732791 31.898804624845962 19.94951911633424 44-45 19.862812394968806 28.8848600524512 31.52900884899305 19.723318703586944 46-47 19.816207733936945 28.84384477954104 30.928436524296682 20.411510962225332 48-49 19.72569818066021 28.808462218977287 31.071805507920082 20.39403409244242 50-51 19.9008260894312 29.046003112800754 30.41118998879313 20.641980808974917 52-53 19.846401595256623 29.349247954654516 30.420619398422605 20.383731051666256 54-55 20.17687790478583 29.012431698279112 30.196082857053135 20.614607539881924 56-57 20.13568576957954 29.19128297931641 29.87923122519559 20.793800025908464 58-59 19.94005455741901 28.987615887897704 30.10560624343365 20.966723311249634 60-61 20.150076628296084 28.916373329721523 30.00894610206604 20.92460393991635 62-63 19.99606475200332 28.74767356676256 30.252292576365647 21.00396910486847 64-65 20.051313982397804 28.596186239629134 30.091494724590916 21.261005053382146 66-67 19.958351917465357 28.854299914279924 29.786475069785563 21.40087309846916 68-69 20.185759546930807 28.36127969868661 29.96190893964753 21.491051814735055 70-71 20.032881256947395 28.29343822617888 30.114667249041165 21.55901326783256 72-73 20.101085002583808 27.979512104632153 30.166791726517907 21.75261116626613 74-75 20.101341735127548 28.19685325324146 29.749860133682756 21.951944877948236 76-77 19.933338960393996 28.137200825214993 30.005368608460614 21.9240916059304 78-79 19.99917571054487 28.06082471141283 29.783049959300705 22.156949618741596 80-81 20.108648720786917 27.962424342337677 29.563893926191092 22.365033010684314 82-83 20.250810061841428 28.030985478746057 29.5936541394111 22.12455032000141 84-85 20.379438285712602 27.574703721766845 29.65893044465531 22.386927547865238 86-87 20.259667613678182 27.614414202228666 29.590920465753022 22.53499771834013 88-89 20.803313060242097 27.39336013726545 29.555654150748563 22.24767265174389 90-91 20.864103433670813 27.222011457838967 29.401091601363788 22.51279350712643 92-93 20.950225822425004 27.008440551749725 29.48382534305231 22.55750828277296 94-95 20.985456455262526 26.912036832520997 29.445107076418648 22.657399635797834 96-97 21.332199877590707 26.62068383308948 29.154958680728193 22.89215760859162 98-99 21.4458349261904 26.39542866789384 28.986825129567368 23.171911276348393 100-101 22.137015921396575 26.004342199065572 29.26698141554511 22.591660463992742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2232.0 1 1319.0 2 435.5 3 434.5 4 344.5 5 362.0 6 545.5 7 890.0 8 1212.5 9 1293.5 10 1083.5 11 842.5 12 849.0 13 953.5 14 1061.5 15 1167.5 16 1373.0 17 1567.0 18 1782.0 19 2091.0 20 2405.0 21 2779.0 22 3364.5 23 4082.0 24 4830.5 25 6079.0 26 8140.0 27 10559.5 28 12298.0 29 14359.5 30 17412.0 31 20615.5 32 23829.5 33 27358.0 34 31639.0 35 35607.0 36 39340.0 37 42556.5 38 42449.5 39 40559.0 40 39230.5 41 38159.0 42 37562.5 43 37983.0 44 38622.5 45 38268.5 46 37338.5 47 36789.5 48 36373.5 49 35288.5 50 33326.0 51 30915.0 52 28528.5 53 25544.5 54 22398.0 55 19297.5 56 16295.0 57 13495.0 58 10758.0 59 8322.0 60 6330.5 61 4703.5 62 3312.0 63 2287.0 64 1584.5 65 1117.0 66 847.0 67 678.0 68 534.5 69 419.0 70 349.0 71 284.0 72 254.0 73 225.0 74 163.0 75 132.5 76 110.5 77 80.0 78 58.5 79 38.5 80 29.0 81 23.5 82 18.5 83 13.0 84 8.0 85 11.0 86 9.0 87 3.5 88 3.5 89 2.0 90 0.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006476200942777859 2 0.10116610866672684 3 0.11254852244494248 4 0.11480538034924385 5 0.15533069836995986 6 0.09625989583128908 7 0.024138567150353837 8 0.02727854336503401 9 0.022274206272887485 10-11 0.049552749637921494 12-13 0.05411552757487862 14-15 0.025855741642757057 16-17 0.029976960424524784 18-19 0.03635503711059389 20-21 0.056519571864243126 22-23 0.03184132130199114 24-25 0.04930743899614961 26-27 0.055243956527029306 28-29 0.052054918183994756 30-31 0.06628293540676429 32-33 0.04906212835437771 34-35 0.061572971084744035 36-37 0.05028868156323715 38-39 0.03027133319465105 40-41 0.034637862618190664 42-43 0.03086007873490358 44-45 0.007212132868093525 46-47 0.013688333810871383 48-49 0.009076493745559877 50-51 0.009616177157458033 52-53 0.009321804387331765 54-55 0.010597419724545587 56-57 0.014129892966060782 58-59 0.018594546646309155 60-61 0.02320638671162066 62-63 0.011823972933405029 64-65 0.009370866515686143 66-67 0.009959612055938675 68-69 0.010842730366317475 70-71 0.029290090627563495 72-73 0.026542611439718342 74-75 0.02806353741870405 76-77 0.022568579043013747 78-79 0.005544020504044682 80-81 0.005102461348855282 82-83 0.005691206889107815 84-85 0.005593082632399059 86-87 0.011873035061759407 88-89 0.03532473241515195 90-91 0.03311693663920496 92-93 0.027916351033640922 94-95 0.0442540397756487 96-97 0.03949501332527406 98-99 0.023991380765290702 100-101 0.0724157014510615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1019116.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.520614391973105 #Duplication Level Percentage of deduplicated Percentage of total 1 76.86865568685619 46.52138269653574 2 13.624603715118816 16.491387753723 3 4.581500081903192 8.318255993809688 4 1.980737105489079 4.7950170629271 5 0.9975276639263375 3.0185493546905806 6 0.5553223732375788 2.016507072836812 7 0.332143759424128 1.4071081070765552 8 0.2077015644565292 1.005618103286652 9 0.13627854387745222 0.7422895083516184 >10 0.6231393623984537 6.83755334374056 >50 0.05440440237163846 2.273483354647827 >100 0.036037754935879976 3.8501999021729842 >500 8.116608353123637E-4 0.294045563612004 >1k 8.116608353123637E-4 1.083537761803138 >5k 3.246643341249455E-4 1.3450644207858158 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7481 0.7340675644381993 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6210 0.6093516341613713 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4053 0.3976976124405857 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3250 0.31890383430345515 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1614 0.15837255032793127 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1103 0.10823105514975724 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.906212835437772E-5 0.0 18-19 0.0 0.0 0.0 9.812425670875543E-5 0.0 20-21 0.0 0.0 0.0 5.887455402525326E-4 0.0 22-23 0.0 0.0 0.0 0.0027474791878451523 0.0 24-25 0.0 0.0 0.0 0.005691206889107815 0.0 26-27 0.0 0.0 0.0 0.011480538034924385 0.0 28-29 0.0 0.0 0.0 0.02639542505465521 0.0 30-31 0.0 0.0 0.0 0.06652824604853619 0.0 32-33 0.0 0.0 0.0 0.13830613983099077 0.0 34-35 0.0 0.0 0.0 0.2554665023412448 0.0 36-37 0.0 0.0 0.0 0.44111759603421 0.0 38-39 0.0 0.0 0.0 0.6745552027443391 0.0 40-41 0.0 0.0 0.0 0.9066681319889002 0.0 42-43 0.0 0.0 0.0 1.1296064432311925 0.0 44-45 0.0 0.0 0.0 1.4153442787670882 0.0 46-47 0.0 0.0 0.0 1.7512726716095126 0.0 48-49 0.0 0.0 0.0 2.0797926830704254 0.0 50-51 0.0 0.0 0.0 2.406497395782227 0.0 52-53 0.0 0.0 0.0 2.7163737984684766 0.0 54-55 0.0 0.0 0.0 3.078991989135682 0.0 56-57 0.0 0.0 0.0 3.503870020684593 0.0 58-59 0.0 0.0 0.0 3.9662805804246037 0.0 60-61 0.0 0.0 0.0 4.413923439529945 0.0 62-63 0.0 0.0 0.0 4.865981890187181 0.0 64-65 0.0 0.0 0.0 5.35773160268311 0.0 66-67 0.0 0.0 0.0 5.9260672975402215 0.0 68-69 0.0 0.0 0.0 6.532916763155519 0.0 70-71 0.0 0.0 0.0 7.191968333339875 0.0 72-73 0.0 0.0 0.0 7.8248697891113475 0.0 74-75 0.0 0.0 0.0 8.48298917885697 0.0 76-77 0.0 0.0 0.0 9.200179371141264 0.0 78-79 0.0 0.0 0.0 9.969473543737905 0.0 80-81 0.0 0.0 0.0 10.784935179116019 0.0 82-83 0.0 0.0 0.0 11.600249628109069 0.0 84-85 0.0 4.906212835437772E-5 0.0 12.405261030147697 0.0 86-87 0.0 9.812425670875543E-5 0.0 13.251926179159193 0.0 88-89 0.0 9.812425670875543E-5 0.0 14.144856915208866 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3110 0.0 51.679104 1 GTATCAA 5635 0.0 41.348606 1 TATCAAC 7205 0.0 32.010155 2 ATCAACG 7310 0.0 31.355211 3 TCAACGC 7390 0.0 30.88708 4 CAACGCA 7555 0.0 30.275454 5 AACGCAG 7655 0.0 29.856474 6 GTGGTAT 1475 0.0 29.014107 1 CATTCCG 225 3.6379788E-12 27.452261 9 ACGCAGA 8510 0.0 26.745121 7 CGCAGAG 8500 0.0 26.720688 8 CCGTATC 135 1.8189894E-12 24.63362 94-95 GCAGAGT 8925 0.0 24.329056 9 GACGTGA 215 1.6552804E-9 24.310444 7 GTATAGT 360 0.0 23.775448 1 CGCCGTA 180 0.0 23.749765 92-93 GCCGTAT 175 0.0 23.075169 94-95 TGGTATC 1805 0.0 22.920393 2 TAGGACG 400 0.0 22.587784 4 GAGTACT 6055 0.0 22.478315 12-13 >>END_MODULE